Novel microRNA pathways of impaired angiogenesis in human peripheral artery disease adipose tissue.

Impaired angiogenic response in peripheral artery disease (PAD) contributes to the progression of tissue ischemia, but methods to evaluate angiogenesis at the tissue level are limited. We describe a novel approach to measure angiogenesis and identify microRNA (miR)-gene pathways utilizing adipose tissue from patients with PAD. Patients with PAD undergoing infrainguinal bypass surgery or non-PAD control patients undergoing knee replacement surgery were recruited.

Dimeric PKM2 induces ferroptosis from intestinal ischemia/reperfusion in mice by histone H4 lysine 12 lactylation-mediated HMGB1 transcription activation through the lactic acid/p300 axis.

Background: The role of Pyruvate kinase M2 (PKM2) in intestinal ischemia-reperfusion (I/R) was investigated in this study, with a focus on the mechanism of dimeric PKM2 in ferroptosis.

Methods: The in vivo and in vitro models of intestinal ischemia-reperfusion were constructed and in vitro and in vivo evaluations were performed using a variety of techniques.

Results: HMGB1 and dimeric PKM2 were highly expressed in intestinal I/R mice.

In vitro assessment of the osteogenic and antibacterial capabilities of Mg-MOF particles with encapsulated Levofloxacin within polydopamine.

Infectious bone defect is a condition where infection and bone defect occur simultaneously. The simultaneous achievement of effective antimicrobial management and enhanced bone regeneration continues to present a major hurdle in musculoskeletal therapeutics. To address these limitations, we have developed a novel osteoconductive material, this material (PDA@Mg-MOF-LEV) consists of Magnesium-based metal-organic frameworks (Mg-MOF) particles loaded with the antibiotic levofloxacin (LEV) and coated with polydopamine (PDA), which integrates photothermal therapy with antibiotic delivery to combat bacterial drug resistance and facilitate bone tissue regeneration.

Regulatory T cells in neurological disorders and tissue regeneration: Mechanisms of action and therapeutic potentials.

Regulatory T cells, a subset of CD4 + T cells, play a critical role in maintaining immune tolerance and tissue homeostasis due to their potent immunosuppressive properties. Recent advances in research have highlighted the important therapeutic potential of Tregs in neurological diseases and tissue repair, emphasizing their multifaceted roles in immune regulation. This review aims to summarize and analyze the mechanisms of action and therapeutic potential of Tregs in relation to neurological diseases and neural regeneration.

Combining a novel ensemble model and multiplex methylation SNaPshot assays for saliva age prediction and cross-platform data analysis.

Background: DNA methylation is a pivotal biomarker for age prediction. However, most studies focus on blood-derived data, with limited research on saliva, and the inability to directly analyze methylation data across diverse platforms constrains predictive accuracy.

Results: We identified 10 age-related CpG sites in saliva (cg00481951, cg07547549, cg10501210, cg13654588, cg14361627, cg15480367, cg17110586, cg17885226, cg19671120, cg21296230) through six Illumina HumanMethylation450 BeadChip datasets and developed two multiplex SNaPshot assays.

Direct Electrosynthesis of an Amino Acid from a Biomass Derivative.

The electrochemical synthesis of nitrogen-containing molecules from biomass-derived compounds under ambient conditions is demonstrated, relying only on green sources of feedstock, renewable energy, and water. In this study, we report a two-step method of electrochemically synthesizing 5-(aminomethyl)furan-2-carboxylic acid (AFCA) from 5-hydroxymethylfurfural (HMF) using hydroxylamine (NHOH) as the nitrogen source in an acidic electrolyte. In the first step, HMF was reductively aminated into (5-(aminomethyl)furan-2-yl)methanol (HMFA) using NHOH as the source of nitrogen.

Trauma-induced recurrent of scalp dermatofibrosarcoma protuberans: a case report with a 6-year follow-up and literature review.

Background: Dermatofibrosarcoma protuberans (DFSP) is a rare mesenchymal cell-derived soft tissue tumor, especially on the scalp. As a result, there is a lack of insight into the etiology of its occurrence and the triggers of its recurrence, which makes diagnosis difficult. This paper reports a case with a long follow-up period in an attempt to provide some valuable evidence-based medicine to assist in the clinical work-up of diagnostic.

White matter characterization in regions of edema surrounding meningioma brain tumor using diffusion MRI: A comparative study of DTI and NODDI.

White matter (WM) tract detection is critical in presurgical planning of tumor resection; however, standard-of-care diffusion tensor imaging (DTI) often fails to characterize white matter tracts through regions of edema. This is because the presence of edema has the effect of increasing the isotropic volume fraction within a voxel and thus marginalizing the anisotropic volume fraction associated with white matter presence and directionality. More recent biophysical models of diffusion, such as neurite orientation dispersion and density imaging (NODDI), account for isotropic and anisotropic volume fractions within voxels by compartmentalizing the diffusion signal based on an assumed tissue microenvironment, e.

Intensity-Based Assessment of Hippocampal Segmentation Algorithms Using Paired Precontrast and Postcontrast MRI.

Hippocampal segmentation is essential in neuroimaging for evaluating conditions like Alzheimer's dementia and mesial temporal sclerosis, where small volume changes can significantly impact normative percentiles. However, inaccurate segmentation is common due to the inclusion of non-hippocampal structures such as choroid plexus and cerebrospinal fluid (CSF), leading to volumetric overestimation and confounding of functional analyses. Current methods of assessment largely rely on virtual or manual ground truth labels, which can fail to capture these inaccuracies.

HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.

Objectives: HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.

Materials And Methods: Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis.

Real-time frame-registered resonant fluorescence scanning laser ophthalmoscopy for quantifying static and dynamic cellular properties in the mouse retina: publisher's note.

This publisher's note contains a correction to Opt. Lett.50, 1329 (2025)10.

Multi-omics analysis to uncover the molecular basis of tumor budding in head and neck squamous cell carcinoma.

Tumor budding (TB) is a prognostic biomarker in HPV-negative and HPV-positive head and neck squamous cell carcinoma (HNSCC). Analyzing TCGA and CPTAC mutation, RNA, and RPPA data and performing proteomics and IHC in two independent in-house cohorts, we uncovered molecular correlates of TB in an unprecedentedly comprehensive manner. NSD1 mutations were associated with lower TB in HPV-negative HNSCC.

Real-time frame-registered resonant fluorescence scanning laser ophthalmoscopy for quantifying static and dynamic cellular properties in the mouse retina.

Fluorescence labeling offers excellent contrast for cell imaging within living mouse eyes. High-speed, high-resolution imaging with a large field of view (FOV) is always desirable. A high-speed scanning laser ophthalmoscopy (SLO) system has been developed, equipped with real-time desinusoiding correction and frame registration for fluorescence imaging of mouse retinas.

Global characterization of extrachromosomal circular DNAs in four body fluids.

Determination of the type of body fluids is highly desired in forensic investigations. Extrachromosomal circular DNA (eccDNA) is a double-stranded, circular type of nucleic acid molecule that exists outside chromosomes and is ubiquitous in eukaryotic cells, which is widely explored in clinical research. The use of eccDNA for body fluid identification has remained relatively unexplored.

Clinical efficacy of different methods for treatment of granulomatous lobular mastitis: A systematic review and network meta-analysis.

Objective: This network meta-analysis aims to evaluate the recurrence rates of various treatment options for granulomatous lobular mastitis.

Methods: We systematically searched and identified eligible studies in PubMed, EMBASE, Cochrane Library, and Web of Science databases until September 30, 2023. Original studies reporting the recurrence rates of various treatments were included.

Expansion Characteristics and Shear Behavior of Reinforced Concrete Beams Under Non-Uniform Expansion Induced by Alkali-Silica Reaction.

Alkali-silica reaction (ASR) is an important factor that seriously affects the durability of reinforced concrete (RC) structures. The current research on alkali-aggregate mainly focuses on the deterioration mechanism of materials and the mechanical properties of standard specimens. However, there is a gap in the field of research on the effect of alkali-aggregate damage on the level of RC structures.

How to Implement Clinical 7T MRI-Practical Considerations and Experience with Ultra-High-Field MRI.

The implementation of clinical 7T MRI presents both opportunities and challenges for advanced medical imaging. This tutorial provides practical considerations and experiences with 7T MRI in clinical settings. We first explore the history and evolution of MRI technology, highlighting the benefits of increased signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and susceptibility at 7T.

Multifunctional all-dielectric quarter-wave plate metasurfaces for generating focused vector beams of Bell-like states.

The generation of vector beams using metasurfaces is crucial for the manipulation of light fields and has significant application potential, ranging from classical physics to quantum science. This paper introduces a novel dielectric metasurface composed of quarter-wave plate (QWP) meta-atoms, known as a QWP metasurface, designed to generate focused vector beams (VBs) of Bell-like states under right circularly polarized illumination. The propagation phase imparted on both the co- and cross-polarized components of the output field constructs hyperbolic and helical phase profiles with topological charge , whereas the Pancharatnam-Berry (PB) phase acts only on the cross-polarized component to construct another helical phase profile with topological charge .

The QIBA Profile for Dynamic Susceptibility Contrast MRI Quantitative Imaging Biomarkers for Assessing Gliomas.

The dynamic susceptibility contrast (DSC) MRI measures of relative cerebral blood volume (rCBV) play a central role in monitoring therapeutic response and disease progression in patients with gliomas. Previous investigations have demonstrated promise of using rCBV in classifying tumor grade, elucidating tumor viability after therapy, and differentiating pseudoprogression and pseudoresponse. However, the quantification and reproducibility of rCBV measurements across patients, devices, and software remain a critical barrier to routine or clinical trial use of longitudinal DSC MRI in patients with gliomas.

Temperature field analysis and compensation improvement of load cell.

During the operation of load cell, heat is generated by the strain gauge and the electronics on the PCB board, which leads to temperature gradients within the sensor itself. These temperature gradients are unstable at different ambient temperatures. Compensation inaccuracies can also occur when compensating for sensor measurements at different temperatures This paper proposes a method to change the position of temperature compensation resistors to address errors caused by the temperature field effect of the strain gauge sensor itself.