[Pharmaceutical process omics and its applications and prospects in drug quality control].

The quality of drugs is a crucial premise for ensuring the safety and effectiveness of clinical medication, while quality control during the pharmaceutical process directly affects the quality and consistency of the final product formulation. However, there is a lack of a comprehensive and scientific system for assessing and optimizing the quality control level during the manufacturing process in the field of drug quality control. Therefore, this study proposed the concept of "pharmaceutical process omics", clarified its advantages in guiding drug production, and explored in depth the research approaches, diverse analytical techniques, and broad range of applications in drug quality control.

Mitochondria-Targeted Ferroptosis Nanodrug for Triple-Negative Breast Cancer Therapy via Fatty Acid Metabolism Remodeling and Tumor Bacterial Symbiosis Inhibition.

Triple-negative breast cancer (TNBC) is considered one of the most aggressive subtypes of breast cancer, due to its pronounced propensity for metastasis. This challenge is amplified by the critical role of mitochondria in metastasis, regulating processes like fatty acid metabolism that drive tumor cell migration. Moreover, emerging evidence suggests that bacterial infiltration, particularly Staphylococcus xylosus (S.

Biocompatible carboxymethyl cellulose-potato starch film with biosynthesized tellurium nanoparticles and undecanoic acid for enhanced antimicrobial protection in post-harvest tomato preservation.

Preserving the post-harvest quality of fruits by minimizing microbial contamination is essential for ensuring food safety. In this study, a novel composite film was developed using carboxymethyl cellulose (CMC) and potato starch (PS), reinforced with tellurium nanoparticles (BP-Te NPs, 93.66 ± 24.

Determinants of carotid intima-media thickness in patients with asymptomatic unstable plaques: a population-based cross-sectional study in rural China.

Purpose: Stroke remains a leading cause of death and disability worldwide, with carotid intima-media thickness (IMT) and carotid plaque being significant predictors of cerebrovascular diseases. Despite the established correlation between carotid IMT and stroke, the specific factors influencing IMT in populations with unstable plaques are not well understood. This study aimed to identify the influential factors affecting carotid IMT in individuals with asymptomatic (without prior cardiocerebrovascular events) unstable plaques and to explore sex-specific differences.

Development of Bio-Based Active Film by the Functional Modification of Pig Collagen and Incorporated With Gallic Acid.

This study investigated the synergistic effects of enzymatic hydrolysis and glycosylation on pig collagen (PC) films and the further enhancement of these films by the incorporation of gallic acid (GA). The aim of the study was to develop bio-based active films with improved functional properties for potential food packaging applications, particularly in preserving pork. The glucose hydrolyze PC (HG-PC) complex film and the GA composites (GA-HG-PC) film were prepared, and their structural, mechanical, water sensitivity, and color properties were thoroughly evaluated.

Xiaoyaosan improves depression-like behaviours in mice with post-stroke depression by modulating gut microbiota and microbial metabolism and regulating P2X7R/NLRP3 inflammasome.

Background: Post-stroke depression (PSD) represents the most prevalent complication of stroke and has been reported to be associated with an imbalance in the gut microbiota. Clinically, Xiaoyaosan (XYS) alleviates depressive symptoms in patients with PSD. However, the existing literature does not provide sufficient evidence to ascertain whether XYS can alleviate these symptoms by modulating gut microbiota.

Genetic determinants of head and neck cancer: exploring causality among immune cells and plasma metabolites through two-sample Mendelian randomization and mediation analysis.

Background: Head and neck cancer represents a major health challenge worldwide, characterized by frequent late-stage diagnoses that adversely affect treatment success. The immune system's cellular components significantly influence the initiation, advancement, and outcome of head and neck malignancies.

Methods: Our investigation employed Mendelian randomization techniques to analyze genome-wide association data encompassing 731 immune cell phenotypes, 1400 plasma metabolites, and 2281 head and neck cancer patients.

Advanced research and exploration of CRISPR technology in the field of directed evolution.

Directed molecular evolution is the key technology for obtaining enzymes, proteins, metabolic pathways, and other components of living organisms that have specific functions or desirable properties, which are indispensable in a variety of industrial and medical applications. Despite the success of traditional methods, they are often limited by low efficiency and the high cost of obtaining desired mutants. The advent of CRISPR technology has significantly advanced the field by enabling precise and efficient gene targeting, offering new prospects for directed evolution.

The Impact of Triglyceride-Glucose Index on Carotid Atherosclerosis: A Prospective Cohort Study in Middle Aged and Elderly Low-Income in Rural Chinese.

Background: Atherosclerotic cardiovascular disease (ASCVD) is a major global health burden. The triglyceride-glucose (TyG) index, as a surrogate marker of insulin resistance, is associated with atherosclerosis, but its role in predicting carotid plaque and intima-media thickness (IMT) changes remains unclear. This study aimed to assess the relationship between the TyG index and carotid plaque incidence and IMT changes over a six-year period in a cohort of middle-aged and elderly individuals with low income and low education in rural China.

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

This study aimed to investigate the therapeutic effects and underlying mechanisms of adipose-derived stem cell exosomes (ADSCs-exo) in ameliorating fibrosis in a rat model. ADSCs were isolated and cultured from rat adipose tissue, and ADSCs-exo were extracted via ultracentrifugation. Urethral fibrosis was induced by local injection of TGF-β1 (10 μg), followed by ADSCs-exo treatment.

Clinical efficacy and psychological influence of lateral rectus approach for treating pelvic fracture with lumbosacral plexus injury.

Background: The surgical treatment of pelvic fractures is challenging, particularly after lumbosacral plexus injuries. Such impairments affect a patient's physiological function and can cause significant mental health problems. In recent years, the new transrectus lateral approach has favorably treated pelvic fractures and possibly preserved patients' physiological and psychological conditions.

Identification of related substances in letrozole using LC-Q-TOF/MS.

Letrozole, a nonsteroidal aromatase inhibitor of the triazole class, is widely used in breast cancer treatment. Its quality and stability directly influence therapeutic efficacy and safety. However, systematic characterization of its related substances (RSs) remains insufficiently documented.

Inherited mitochondrial genetics as a predictor of immune checkpoint inhibition efficacy in melanoma.

Response to immune checkpoint inhibitors (ICIs) in metastatic melanoma (MM) varies among patients, and current baseline biomarkers predicting treatment outcomes are limited. As mitochondrial (MT) metabolism has emerged as an important regulator of host immune function, we explored the association of host MT genetics (MT haplogroups) with ICI efficacy in 1,225 ICI-treated patients with MM from the clinical trial CheckMate-067 and the International Germline Immuno-Oncology Melanoma Consortium. We discovered and validated significant associations of MT haplogroup T (HG-T) with resistance to anti-programmed cell death protein-1-based ICI (both single-agent and combination) and have shown that HG-T is independent from established tumor predictors.

Long-Term Benefits of N-Butylphthalide in Preventing Ischemic Stroke Recurrence: A 12-Month Prospective Study.

Purpose: This study evaluated the effects of 12 months of NBP treatment on stroke recurrence and examined the influence of age and gender on its efficacy.

Methods: A prospective cohort of 1109 patients with non-cardioembolic ischemic stroke (IS) within six months was divided into NBP (n = 538) and control (n = 571) groups. The NBP group received NBP plus standard treatment, while the control group received standard treatment alone.

Quantitative Evaluation of Acute Pancreatitis Based on Dual-Energy Computed Tomography.

Objective: To evaluate the value of dual-energy computed tomography (DECT) parameters for the quantitative diagnosis of acute pancreatitis (AP) and classification of its severity.

Methods: Patients with AP underwent a plain CT scan and three contrast-enhanced DECT scans. We analyzed the group differences in iodine concentration (IC) and slope of the spectral Hounsfield unit curve (λHU) of the 3-phase enhanced scans (arterial, venous, and delayed phases).

Crosstalk between kidney and bones: New perspective for modulating osteoporosis.

Growing evidence indicates an interesting interplay between kidney and bone. The pathophysiological condition of the skeletal system is intricately associated with the normal functioning of the kidneys. This relationship is modulated by various factors, including calcium and phosphate, 1-α-hydroxylase, erythropoietin (EPO), klotho, fibroblast growth factor 23 (FGF23), bone morphogenetic protein-7 (BMP-7), and extracellular vesicles (EVs).

Correlations Between TyG-Related Indices and Bone Health: A Cross-Sectional Study of Osteoporosis in a Rural Chinese Population.

Background: Osteoporosis (OP) is a major metabolic bone disease with significant health and socioeconomic impacts. The triglyceride-glucose (TyG) index and its derivatives, which reflect insulin resistance (IR), may play a role in bone metabolism. However, the relationship between TyG indices and OP is unclear.

A hyaluronic acid Schiff base @ hollow calcium peroxide @ copper carbon dots nanoconjugate enhances chemodynamic tumor therapy based on multiple approaches strategy.

Chemodynamic therapy (CDT) is a highly promising cancer treatment strategy. However, its clinical application is severely limited due to insufficient HO levels. To overcome this critical issues, based on self-supply HO strategy this study designed a multifunctional nano-reactor hyaluronic acid (HA)-cinnamaldehyde(CA)Schiff base(HA-CA)@hollow CaO(HMCaO)/Copper doped luteolin carbon dots (CuLCDs) (abbreviation:HACOCLC).

Magnetic graphene oxide nanocomposite materials for magnetic dispersive solid-phase extraction and determination of pyrrolizidine alkaloids in tea beverages.

Pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) are plant-derived toxins, and tea beverages represent one of the most important exposure pathways of PAs/PANOs for human being. Therefore, establishing a method for the sensitive determination of PAs/PANOs is of great significance. In this study, magnetic graphene oxide (MGO) nanocomposite materials were used as an adsorbent in magnetic dispersive solid-phase extraction (d-MSPE) for quantifying PAs/PANOs in tea beverages.

Glucose oxidase/copper‑carbon dots/hyaluronic acid self-assembly for self-supply hydrogen peroxide in a double-enzyme cascade to enhance anti-tumor therapy.

Although chemodynamic therapy (CDT) has proven to be a promising anti-tumor strategy, its efficacy is limited by the insufficient supply of HO in tumor tissues. To solve the problem of insufficient HO, in this paper, a novel double-enzyme cascade nanoreactor hyaluronic-cinnamaldehyde Schiff base@glucose oxidase (GOx)/copper doped carbon dot (abbreviation HCFCTG), which constructed by co-assembly of copper doped carbon dot (CuFACDs-TPP), glucose oxidase (GOx) and hyaluronic-cinnamaldehyde Schiff base (HA-CA) was designed for the first time. The HCFCTG released GOx and CuFACDs-TPP under pH stimulation.

Pirfenidone Ameliorates Hypertrophic Scar Through Inhibiting Proliferation and Migration of Fibroblasts by Regulating the Wnt/GSK-3β/β-Catenin Signaling Pathway.

Hypertrophic scar (HS) is a prevalent complication that arises from burn injuries. While Pirfenidone (PFD) is known to be an effective antifibrotic agent, its precise effects on HS caused by burn injuries are still unclear. This study was to assess the influence of PFD on HS fibroblasts and investigate the underlying mechanism.

Identification of related substances in minocycline hydrochloride via 2D-LC-Q-TOF /MS.

Minocycline hydrochloride is a semisynthetic tetracycline derivative with a wide range of applications. The related substances significantly influence its efficacy and safety. However, the investigation of potential related substances in minocycline hydrochloride or its products remains incomplete.

Decoding the pharmacodynamics of Fufang Tongye Shaoshang You: A promising ethnomedicine for diabetic ulcer healing.

Ethnopharmacological Relevance: Fufang Tongye Shaoshang You (TYY) is an ethnomedicine derived from the traditional folk formula of the Tujia people in Hunan Province, which consists of Paulownia leaf and sesame oil, has shown promising potential in promoting diabetic ulcer (DU) healing. However, its pharmacological substance and mechanism of action require further elucidation.

Aims Of The Study: This study was designed to assess the healing effect of TYY on DU wounds in mice, and to explore systematically its potential mechanisms and pharmacodynamic material basis.

Quantitative H NMR optimization for high-throughput metabolite analysis in industrial bioprocess monitoring.

Quantitative H NMR (H qNMR) is an ideal tool for bioprocess monitoring because it can comprehensively detect and quantify diverse metabolites that significantly influence bioprocess performance. However, the long experiment time associated with the H qNMR, due to the long longitudinal relaxation time (T1) of some metabolites, does not meet the requirements for high-throughput analysis. We developed a high-throughput H qNMR method for bioprocess analysis using a short relaxation delay (D1) to reduce analytical time and a correction factor (k) to compensate for incomplete relaxation.

Impacts of the COVID-19 Pandemic on Wildlife in Huangshan Scenic Area, Anhui Province, China.

Human activities impact ecosystems globally, and understanding human-wildlife coexistence is crucial for species conservation. This study analyzed trends in local wildlife populations before and during the COVID-19 pandemic to assess their response to human disturbance. From 2017 to 2022, 60 camera sites were monitored, and seven species with the largest population size-excluding rodents-were selected for analysis.