TRIM2 inhibits apoptosis by ubiquitinating BNIP3 to protect the intestine against ischemia-reperfusion injury in mice.

Intestinal damage following interrupted blood flow and its return (intestinal ischemia/reperfusion injury) is a serious medical problem occurring in various clinical situations. While the death of intestinal epithelial cells is a key factor, the precise reasons behind this cell death are not fully known. In this study, we identified significant downregulation of an E3 ubiquitin ligase TRIM2 in mouse models of this injury and in cells mimicking the condition.

Short-course-based TNT with or without PD-1 inhibitor for pMMR locally advanced rectal cancer: Phase 2 results of a randomized trial (STELLAR II).

Background: The therapeutic efficacy and mode of combining immunotherapy with neoadjuvant chemoradiotherapy in proficient mismatch repair (pMMR)/microsatellite stable (MSS) locally advanced rectal cancer (LARC) remain uncertain.

Methods: In this multicenter, randomized, seamless phase 2/3 trial (ClinicalTrials.gov: NCT05484024), eligible participants were randomly assigned (1:1) to receive short-course radiotherapy (SCRT) (5 Gy × 5), followed by 4 cycles of capecitabine and oxaliplatin or 6 cycles of leucovorin, oxaliplatin, and fluorouracil, with (iTNT group) or without (total neoadjuvant therapy [TNT] group) 4 cycles of sintilimab.

Engineered crRNA Drives RPA-T7-CRISPR/Cas14a Cascade for Ultrasensitive Detection of ctDNA PIK3CA H1047R.

The early detection of circulating tumor DNA (ctDNA) at mutant allele frequencies below 0.1% remains a critical challenge, significantly impeding therapeutic decision-making. To address this limitation, TIDE-Cas14a-an innovative CRISPR/Cas14a-based duplex detection system is developed that integrates recombinase polymerase amplification (RPA) with T7 exonuclease-mediated strand displacement.

Integration of single cell multiomics data by deep transfer hypergraph neural network.

Multi-omics characterization of individual cells offers remarkable potential for analyzing the dynamics and relationships of gene regulatory states across millions of cells. How to integrate multimodal data is an open problem, existing integration methods struggle with accuracy and modality-specific biological variation retention. In this paper, we present scHyper (scalable, interpretable machine learning for single cell integration), a low-code and data-efficient deep transfer model designed for integrating paired and unpaired single-cell multimodal data.

Awake Flexible Bronchoscopy Intubation Practice Among Otorhinolaryngology Surgery: An Observation Study Based on Practice Video Recordings and Clinical Data.

Background: Patients with airway pathologies are at higher risk for difficult intubation, making Awake flexible bronchoscopy intubation (AFBI) an essential technique. This study aimed to describe the baseline characteristics, procedural details, and outcomes of AFBI regarding otorhinolaryngology surgery.

Methods: This single-centre, observational study included 147 adult patients who underwent a standardised AFBI protocol for otorhinolaryngology surgery patients at Eye & ENT Hospital of Fudan University between January 1, 2022 and July 31, 2023.

New cardiac glycosides from Adonis amurensis with potent antitumor activity.

Twenty-four cardiac glycosides - including six previously undescribed compounds (1-6) and eighteen known analogues (7-24) - were isolated from the whole herb of Adonis amurensis. Their structures were elucidated through comprehensive spectroscopic analysis (1D/2D NMR, HRMS) and acid hydrolysis. All compounds exhibited potent in vitro cytotoxicity against diverse cancer cell lines, with IC₅₀ values ranging from 0.

The Relationship Between Cancer Cognition, Coping Ability and Behavioral Intention of Secondary Cancer Prevention Among Rural Residents in Shandong Province.

Background: Secondary prevention plays a crucial role in reducing cancer-related deaths. Previous studies have indicated that cancer cognition and coping ability significantly influence behavioral intention towards secondary prevention. However, limited research has explored the relationship between the three, particularly among rural residents.

T Cell Involvement in Neuroinflammation After Traumatic Brain Injury: Implications for Therapeutic Intervention.

Background: Traumatic brain injury (TBI) is a leading cause of death and disability across all age groups worldwide. After primary mechanical head injury, a cascade of molecular changes and immunological responses occur that are necessary for supporting tissue repair but also exacerbate the secondary loss of tissue caused by excessive neuroinflammation. To date, there are no targeted treatments that ameliorate the pathological neuroinflammation that is responsible for propagating secondary injury after TBI.

MnO/Pt Oxide-on-Metal Inverse Catalyst Facilitates Hydrogen Spillover for CO Hydrogenation Reaction.

Hydrogen spillover, the migration of metal-activated hydrogen species across support surfaces, is key for many H-related reactions. However, questions remain about how the metal/oxide interfaces affect hydrogen spillover and hydrogenation reaction. Here, we construct MnO-on-Pt(111) (MnO/Pt(111)) inverse catalyst and Pt clusters-on-MnO (Pt/MnO) catalyst, and image hydrogen spillover behavior using high-pressure scanning tunneling microscopy.

Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review.

Extrachromosomal circular DNA (eccDNA) is a class of chromosome-independent circular DNA molecules found in diverse organisms, including plants, animals, and microorganisms. Recent research has highlighted its roles in gene regulation, genome stability, and disease pathogenesis, with growing recognition of eccDNA as a valuable biomarker for cancer diagnosis, prognosis, and monitoring in precision medicine. Studies have also linked eccDNA to non-neoplastic diseases and normal tissue biology, broadening its biological significance beyond malignancies.

The Volatile Oil of Dried Ginger Promotes Intestinal Epithelial Renewal through T-UCR 481.

Dried ginger is widely used in condiments and in food materials. The volatile oil in ginger can regulate intestinal flora and affect the intestinal barrier function. First, we extracted the volatile oil of dried ginger (VOG) and analyzed it by gas chromatography-mass spectrometry (GC-MS).

Psychological health status and influencing factors among aesthetic plastic surgery patients: A cross-sectional analysis.

Objective: This study explored psychological well-being and body image-related distress among patients undergoing aesthetic plastic surgery, and identified key demographic and clinical factors influencing their mental health and quality of life.

Methods: A cross-sectional survey was conducted among 403 outpatients and inpatients at a tertiary plastic surgery hospital. Participants completed a validated five-dimensional psychological assessment scale, comprising appearance concern, emotional distress, cognitive distress, behavioural distress, and body image inferiority.

Research note: Application of convolutional neural networks for feather classification in chickens.

Feather color plays a crucial role in distinguishing various poultry breeds. However, the reliability of conventional manual classification methods is debated due to the intricate nature of feather color traits. To address this issue, we applied Convolutional Neural Networks (CNN) to extract the feather texture features of 300 images each of Golden Meihua (GM) and Silver Meihua (SM) feathers.

Two-dimensional nanomaterials for bone disease therapy: multifunctional platforms for regeneration, anti-infection and tumor ablation.

Bone diseases, encompassing defects, infections, necrosis, osteoarthritis and tumors, present persistent clinical challenges, often unmet by conventional therapies due to limited efficacy and biocompatibility. Two-dimensional (2D) nanomaterials have emerged as powerful platforms with tunable physicochemical properties that enable efficient drug loading, controlled release and dynamic cellular modulation. Recent advances in 2D structures such as graphene, black phosphorus, layered double hydroxides, transition metal disulfides and MXenes demonstrate broad therapeutic potential across orthopedic applications.

Application of a multiple transmitter spacing gradient array TDIP survey in the Huaniushan mining area, Gansu province, China.

Time domain induced polarization (TDIP) method is an important geophysical exploration technique for metal exploration. It is common to collect resistivity/IP data using a gradient array. However, the depth of investigation of a typical gradient array survey is limited by a single fixed transmitter electrode distance, which only provides information over a limited range of depths.

Structural characterization of two glucans from Saposhnikovia divaricata and evaluation of their anti-neuroinflammatory activity.

Saposhnikovia divaricata (Turcz.) Schischk. has been widely used as an important Chinese herbal medicine in the field of medicine, agriculture and other fields where it has been demonstrated to have significant economic value.

Neuromyelitis optica spectrum disorders with recurrent syncope due to area postrema syndrome: a case report and literature review.

Background: Area postrema syndrome is one of the core clinical features of neuromyelitis optica spectrum disorders (NMOSD), characterized by intractable hiccups, nausea, and vomiting. Brain MRI often reveals lesions in the dorsal medulla oblongata. However, recurrent syncope has rarely been described in NMOSD.

A breakthrough infection in an immune thrombocytopenia patient during caspofungin and isavuconazole combined therapy: a case report.

infection is a rare but highly lethal infectious disease. Clinically, is the strain most commonly isolated from patients with infections. is an opportunistic pathogen that can cause local or invasive infections in immunocompromised patients.

Non-Native Woody Plant Species Show Different Leaf Functional Traits and Herbivory Levels From Native Ones in the Urban Areas of Beijing, China.

A large number of non-native species have been introduced to urban ecosystems, and it is a distinctive feature of the urbanization process. However, it is unclear whether these non-native species have similar functional traits to native ones and are similarly integrated into the local food web. We evaluated the differences in leaf functional traits and herbivory between native and non-native species of common woody plants in 50 parks in Beijing, China.

USP3 stabilizes MIC19 by deubiquitination under hypoxic stress and promotes the progression of non-small cell lung cancer.

Hypoxia is a common phenomenon in the microenvironment of solid tumors; mitochondria, as the site of cellular oxidative respiration, are among the first organelles to be affected under hypoxic conditions. Mitochondrial cristae organizing protein 19 (MIC19), a core component of the mitochondrial contact site and cristae organizing system (MICOS), is essential for preserving mitochondrial activity. In this study, we investigated the effects of hypoxia on MIC19 and its regulatory mechanisms in non-small cell lung cancer (NSCLC).

Profiling of RUVBL2-Induced Transcriptome Alterations Highlights a Critical Role for Chromatin Remodeling in Ovarian Cancer.

Cancerous transcriptome alterations in carcinoma cells could be originated from either genetic copy number changes or epigenetic reprogramming. Ovarian cancer (OV) is the most malignant gynecologic tumor, known for high aneuploidy with robust copy number alterations. However, low aneuploidy ovarian tumors are also frequently found, indicating an essential contribution of epigenetic factors during tumorigenesis and cancer development.

Investigating the mechanism of anti-hepatic fibrosis action of Chiyin Heji based on network pharmacology, molecular docking and experimental validation.

To unravel the pharmacological mechanisms and validate the efficacy of Chiyin Heji (the CYP) in anti-hepatic fibrosis, we analysed its components using UHPLC-Q-Orbitrap HRMS, screened drug targets through TCMSP, and identified disease targets using databases such as Genecard, OMIM, Drugbank and TTD. The intersection of these targets was analysed using GO and KEGG for PPI network analysis and target prediction. Molecular docking was simulated using AutoDock Vina.

Annexin A2: regulating glioma's fate and a potential therapeutic target.

High recurrence and mortality in glioma are linked to aggressive proliferation. Recent strategies focus on genetic interventions, with Annexin A2 (ANXA2) emerging as a promising target. We wanted to evaluate ANXA2's prognostic value and its role in tumor progression, aiming to establish it as a viable therapeutic target.

Inner Ear Organoid as a Preclinical Model of Hearing Regeneration: Progress and Applications.

Hearing loss is one of the most common health problems and there is no suitable way to regenerate up to now. However, if the mechanism can be clearly clarified, physiological auditory regeneration may be a possibility. Organoids, artificially generated by pluripotent stem cells under three-dimensional (3D) culture system, are now considered attractive options for constructing different 3D models to study auditory regeneration and the potential mechanisms in vitro.

Activation of S1PR1 by Ponesimod for Multiple Sclerosis Therapy: Uncovering MAPK and PI3K Pathway Mechanisms and Repurposing Potential.

Multiple sclerosis (MS), affecting over 2 million people globally, primarily causes neurological disability in individuals under 40. This autoimmune disorder involves the immune system attacking the myelin sheath in the central nervous system. Sphingosine-1-phosphate receptor 1 (S1PR1) is a promising therapeutic target, with approved drugs like ponesimod acting as S1PR1 agonists.