Sequential treatment with PARPi and WEE1i enhances antitumor immune responses in preclinical models of ovarian cancer.

The antitumor activity demonstrated by DNA damage response inhibitors (DDRis) can be partially attributed to their capacity to enhance immune responses. However, the toxicity of DDRis to lymphocytes, particularly when a DDRi is combined with other treatments targeting cell cycle checkpoint kinases, indicates a need for the development of different DDRi treatment schedules. Here, we systematically assessed changes to the tumor immune microenvironment (TIME) in response to DDRis across various treatment timelines in ovarian cancer.

Beyond Stress Granules: G3BP1 and G3BP2 Redundantly Suppress SARS-CoV-2 Infection.

The global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed unprecedented challenges to public health and economic stability. Central to SARS-CoV-2 pathogenesis is its ability to evade the host immune response by hijacking host pathways via the interaction between viral and host proteins. We identified Ras-GTPase-activating protein SH3 domain-binding protein 1/2 (G3BP1/G3BP2) as a critical host factor that interacts with the viral nucleocapsid (N) protein, emerging from a comparative analysis of proteomic data from multiple studies.

Local Coordination Environment-Driven Structural Dynamics of Single-Atom Copper and the CO Electroreduction Pathway.

Structural stability is a critical factor in realizing the potential of single-atom catalysts (SACs), yet remaining a major challenge hindering their large-scale application. Understanding the structural dynamics of SACs is essential for elucidating the structure-activity relationship and guiding the design of high-performance SACs. In this study, we selected five well-defined mononuclear copper (Cu) complexes with varying ligand structures to explore the coordination-driven structural dynamics of Cu single atoms and their interaction with the electrochemical CO reduction (COR) pathway.

ATR-mediated phosphorylation of RIPK1 inhibits DNA damage-induced necroptosis.

Necroptosis induced by DNA damage during chemotherapy is a significant and effective treatment strategy for epithelial ovarian cancer. Ataxia telangiectasia and rad3-related protein (ATR), a key kinase in DNA damage checkpoints, initiates repair by transmitting damage signals to effectors. However, persistent DNA damage may result in cell death.

Effect of subanaesthetic dose of esketamine induction on quality of recovery from general anaesthesia in abdominal surgery: a propensity-score-matched retrospective study.

Objectives: Subanaesthetic doses of esketamine may attenuate the opioid-induced cough reflex and prevent intraoperative haemodynamic fluctuations. This study aims to evaluate the effect of subanaesthetic doses of esketamine on the quality of recovery in patients who underwent abdominal surgery.

Design: Retrospective cohort study using propensity score matching (PSM) methodology.

Autonomous Self-Evolving Research on Biomedical Data: The DREAM Paradigm.

In contemporary biomedical research, the efficiency of data-driven methodologies is constrained by large data volumes, the complexity of tool selection, and limited human resources. To address these challenges, a Data-dRiven self-Evolving Autonomous systeM (DREAM) is developed as the first fully autonomous biomedical research system capable of independently conducting scientific investigations without human intervention. DREAM autonomously formulates and evolves scientific questions, configures computational environments, and performs result evaluation and validation.

Mitigating T cell DNA damage during PARP inhibitor treatment enhances antitumor efficacy.

Poly(ADP-ribose) polymerase inhibitors (PARPis) are a class of agents targeting DNA damage repair that have become standard therapy for epithelial ovarian cancer (EOC) and multiple other solid tumors. In addition to targeting DNA damage repair, PARPis actively modulate antitumor immune responses, with efficacy being partially dependent on T cell activity. Here, we found that patient T cells sustain DNA damage during PARPi treatment, which reduces treatment efficacy.

Related factors affecting misdiagnosis of aortic dissection: a single-center retrospective study.

Objective: Aortic dissection (AD) is a life-threatening cardiovascular emergency. Delayed diagnosis frequently leads to treatment delays, elevated mortality, and complications. This study investigates the factors contributing to the misdiagnosis of AD and proposes strategies for improving its early diagnosis.

Advancements in microwave ablation for tumor treatment and future directions.

Microwave ablation (MWA) is a minimally invasive treatment that uses thermal energy to target and destroy tumors. Compared to other ablation methods, such as radiofrequency ablation (RFA), MWA operates at higher frequencies, allowing for faster ablation and larger treatment areas. In addition to its direct tumor-destroying effects, MWA has been shown to activate immune responses, contributing to long-term antitumor effects.

Withdrawal notice to: ATR-mediated phosphorylation of RIPK1 inhibits DNA damage-induced necroptosis.

This article has been withdrawn at the request of the editor and publisher. The publisher regrets that an error occurred which led to the premature publication of this paper. This error bears no reflection on the article or its authors.

ERCC3 serves as a prognostic biomarker for hepatocellular carcinoma and positively regulates cell proliferation and migration.

Background: ERCC3, a crucial component of the nucleotide excision repair pathway, is implicated in the development and progression of various cancers and is a potential indicator of poor prognosis. However, the expression and function of ERCC3 in hepatocellular carcinoma (HCC) remain unclear. This study aimed to investigate the expression of ERCC3 in HCC tissues and its clinical significance, focusing on elucidating its potential mechanisms and therapeutic value in immunotherapy.

Organoid models: applications and research advances in colorectal cancer.

This review summarizes the applications and research progress of organoid models in colorectal cancer research. First, the high incidence and mortality rates of colorectal cancer are introduced, emphasizing the importance of organoids as a research model. Second, this review provides a detailed introduction to the concept, biological properties, and applications of organoids, including their strengths in mimicking the structural and functional aspects of organs.

Hypoxia induces ferroptotic cell death mediated by activation of the inner mitochondrial membrane fission protein MTP18/Drp1 in invertebrates.

Hypoxia and ischemia damage sensitive organelles such as mitochondria, and mitochondrial dysfunction contributes to metabolic disorders in crustaceans under hypoxia. The mechanisms associated with ferroptosis in hypoxic disorders have not been determined in crustaceans. In particular, the early molecular events of mitochondrial dynamics in crustaceans require clarification.

An electrochemiluminescence biosensor based on metal porphyrin luminophore and covalent organic framework for the sensitive detection of ctDNA in non-small cell lung cancer.

The development of electrochemiluminescence (ECL) sensors for the sensitive detection of circulating tumor DNA (ctDNA) associated with non-small cell lung cancer (NSCLC) offers a promising approach for early diagnosis; however, the lack of robust and efficient luminophore remains a key limitation to the analytical performance of ECL sensors. Herein, a ECL biosensor is developed using a novel His@ZIF-8/Fe-TCPP (HZTCP) luminophore for the sensitive detection of NSCLC-related ctDNA. The HZTCP luminophore, synthesized using a histidine imidazole framework (His@ZIF-8) as the precursor and tetra-(4-carboxyphenyl) porphyrin ferric chloride (Fe-TCPP) as the luminescent ligand, integrates the photoelectrochemical activity of porphyrin with the porous structure of the MOF, achieving excellent ECL performance.

Machine learning-based bulk RNA analysis reveals a prognostic signature of 13 cell death patterns and potential therapeutic target of SMAD3 in acute myeloid leukemia.

Background: Dysregulation or abnormality of the programmed cell death (PCD) pathway is closely related to the occurrence and development of many tumors, including acute myeloid leukemia (AML). Studying the abnormal characteristics of PCD pathway-related molecular markers can provide a basis for prognosis prediction and targeted drug design in AML patients.

Methods: A total of 1394 genes representing 13 different PCD pathways were examined in AML patients and healthy donors.

In Situ Self-Assembly of Artificial Topological Nanostructures Enhances In Vivo Efficacy of PCSK9 Inhibitory Peptides.

Optimizing the stability and affinity of peptides in vivo is critical for their development as alternatives to approved monoclonal antibodies. In recent years, efforts in academia and industry have focused on modifying Pep2-8, a classical antagonistic peptide targeting proprotein convertase subtilisin/kexin type 9 (PCSK9), to enhance its specificity and affinity. However, developing effective PCSK9 inhibitory peptides remains challenging, especially given the limited examples of their successful in vivo applications.

Berberine attenuates obesity-induced skeletal muscle atrophy via regulation of FUNDC1 in skeletal muscle of mice.

Skeletal muscle atrophy is a complication of obesity, partially induced by impaired mitophagy. This study investigates whether Berberine(BBR) protects mice from obese skeletal muscle atrophy and the underlying molecular mechanism. Twenty C57BL/6 mice were fed a high-fat diet until they weighed more than 20% of the average body weight of the control group.

MicroRNAs in pancreatic cancer drug resistance: mechanisms and therapeutic potential.

Pancreatic cancer (PC) remains one of the most lethal malignancies, primarily due to its intrinsic resistance to conventional therapies. MicroRNAs (miRNAs), key regulators of gene expression, have been identified as crucial modulators of drug resistance mechanisms in this cancer type. This review synthesizes recent advancements in our understanding of how miRNAs influence treatment efficacy in PC.

Correlation of volatile organic chemical exposure with uric acid metabolism among US population: A cross-sectional study from NHANES program.

Volatile organic chemicals (VOC) have been identified as hazardous pollutants impairing human health. While whether the VOC exposure was associated with hyperuricemia is yet clarified. All the data was acquired from 6 survey cycles of the National Health and Nutrition Examination Survey (NHANES) program (2005-2006, 2011-2012, 2013-2014, 2015-2016, 2017-2018, 2017-2020).

Viral proteins resolve the virus-vector conundrum during hemipteran-mediated transmission by subverting salicylic acid signaling pathway.

Hemipteran insects transmit viruses when infesting plants, during which vectors activate salicylic acid (SA)-regulated antiviral defenses. How vector-borne plant viruses circumvent these antiviral defenses is largely unexplored. During co-infections of begomoviruses and betasatellites in plants, betasatellite-encoded βC1 proteins interfere with SA signaling and reduce the activation of antiviral resistance.

RT-DETR-SoilCuc: detection method for cucumber germinationinsoil based environment.

Existing seed germination detection technologies based on deep learning are typically optimized for hydroponic breeding environments, leading to a decrease in recognition accuracy in complex soil cultivation environments. On the other hand, traditional manual germination detection methods are associated with high labor costs, long processing times, and high error rates, with these issues becoming more pronounced in complex soil-based environments. To address these issues in the germination process of new cucumber varieties, this paper utilized a Seed Germination Phenotyping System to construct a cucumber germination soil-based experimental environment that is more closely aligned with actual production.

YOLOv8-segANDcal: segmentation, extraction, and calculation of soybean radicle features.

The high-throughput and full-time acquisition of images of crop growth processes, and the analysis of the morphological parameters of their features, is the foundation for achieving fast breeding technology, thereby accelerating the exploration of germplasm resources and variety selection by crop breeders. The evolution of embryonic soybean radicle characteristics during germination is an important indicator of soybean seed vitality, which directly affects the subsequent growth process and yield of soybeans. In order to address the time-consuming and labor-intensive manual measurement of embryonic radicle characteristics, as well as the issue of large errors, this paper utilizes continuous time-series crop growth vitality monitoring system to collect full-time sequence images of soybean germination.