Observation on the therapeutic effect of facial acupuncture combined with scalp Gua Sha in the treatment of facial paralysis.

Facial paralysis is a common neurological disorder that can result from various central or peripheral nervous system diseases, impairing facial expression and significantly affecting the quality of life. Traditional Chinese external therapies, including facial acupuncture and scalp Gua Sha, have shown promise in rehabilitation. However, clinical evaluations of their combined application remain limited.

Environmental fate of POPs under climate change: Regulation of POPs distribution by soil organic matter and microbial communities during glacial retreat.

Glacial retreat zones are highly sensitive to climate change. This makes them unique models of vegetation succession that reflect interactions among soil development, microbes, and the behavior of chemicals. In this study, the fate of persistent organic pollutants (POPs) during soil succession in glacial retreat areas was investigated, with a focus on the roles of soil organic matter (SOM) and microbial communities.

An attack detection method based on deep learning for internet of things.

With the rapid development of Internet of Things (IoT) technology, the number of network attack methods it faces is also increasing, and the malicious network traffic generated is growing exponentially. To identify attack traffic for the protection of IoT device security, attack detection has attracted widespread attention from researchers. However, current attack detection methods struggle to identify complex and variable attack methods, resulting in a high false positive rate.

Tumor microenvironment in osteosarcoma: From cellular mechanism to clinical therapy.

Osteosarcoma, a prevalent primary malignant bone tumor, predominantly affects both elderly and adolescent populations and usually has an unfavorable prognosis. The specific mechanisms underlying its invasive progression remain unclear. The tumor microenvironment includes not only cancer cells but also bone-related cells, immune cells, tumor-associated nerve cells, and cell-secreted factors.

Long noncoding RNA TUG1 promotes chondrosarcoma progression and M2 polarization.

The long non-coding RNA taurine up-regulated gene 1 (TUG1) has been reported to be involved in various cancers, but its role in chondrosarcoma (CHS) remains a mystery. This research aimed to examine the function of TUG1 in CHS. We found that TUG1 expression was elevated in CHS.

KPNA2 promotes osteosarcoma progression by regulating the alternative splicing of DDX3X mediated by YBX1.

Osteosarcoma (OS) is a rapidly progressive primary malignant bone tumor that occurs in children and adolescents aged between 15 and 19 years and adults aged over 60 years. As alternative splicing (AS) changes caused by abnormal splicing factors contribute to tumor progression, gene expression and AS analyses were performed on 44 osteosarcoma patients to create a genome-wide co-expression network of RNA-binding proteins (RBPs), AS events, and AS genes. A gain- or loss-of-function osteosarcoma cell model was established, and an interactive network analysis and enrichment analysis were performed.

Epidemiological trends and characteristics of osteoarthritis in China during 1990-2021.

Background: This study aimed to comprehensively analyze the incidence, prevalence, and disability-adjusted life years (DALYs) of osteoarthritis (OA) in China from 1990 to 2021 by age, sex, joint sites, high body mass index (BMI) and sociodemographic index (SDI).

Methods: Data and methodologies from the Global Burden of Diseases (GBD) Study 2021 were obtained to evaluate the burden of OA in China. This assessment was conducted by estimating the number of incident cases, prevalent cases, DALYs, and corresponding age-standardized rates (ASRs).

Nucleus pulposus cell-mimicking nanoparticles for cell-specific HIF1A editing to modulate SASP-mediated disc inflammation via autophagy activation.

Traditional methods of plasmid delivery, including viral vectors, lipofection, and electroporation, are widely used for gene editing but have limitations, such as cellular toxicity, limited transfection efficiency in primary cells, and nonspecific side effects. Here, we report the development of nucleus pulposus cell (NPC)-mimicking nanoparticles (HIF1A@NNP) with an NPC membrane as the shell and pcDNA3.1-rHIF1A encapsulated in the core via extrusion.

Fructose 1,6-bisphosphatase 1 is a potential biomarker affecting the malignant phenotype and aerobic glycolysis in glioblastoma.

Background: Fructose 1,6-bisphosphatase 1 () has been considered as a potential prognostic biomarker in glioblastoma (GBM), and this study explored the underlying mechanism.

Methods: The expression and effect of expression on the prognosis of GBM patients were examined applying bioinformatics analyses. After measuring the expression of in normal glial cell line HEB and GBM cells, cell counting kit-8 (CCK-8), 5-ethynyl-2-deoxyuridine (EdU), colony formation, transwell, and wound healing assay were carried out to examine the effects of silencing on the proliferation and invasion of GBM cells.

P/P═O Redox Catalysis Mediated Thioesterification of Carboxylic Acids with Disulfides under Air Conditions.

An efficient organophosphorus-catalyzed thiocarbonylation reaction of disulfides with carboxylic acids under air conditions was described. Various functional groups on carboxylic acids and disulfides can be tolerated under the present reaction conditions, affording thioesters in good to excellent yields. This method exhibited excellent chemoselectivity and can be applied for the late-stage functionalization of drug molecules containing a carboxylic acid group.

Signaling pathways and targeted therapies in Ewing sarcoma.

Ewing sarcoma, the second most prevalent malignant bone tumor with potential occurrence in soft tissues, exhibits a high level of aggressiveness, primarily afflicting children and adolescents. It is characterized by fusion proteins arising from chromosomal translocations. The fusion proteins induce aberrations in multiple signaling pathways and molecules, constituting a key event in oncogenic transformation.

Tracking Microplastics Contamination in Drinking Water Supply Chain in Haikou, China: From Source to Household Taps.

The presence of microplastics (MPs) in aquatic environments has become a significant global concern due to their potential adverse effects on human health. This study aimed to investigate the contamination of MPs throughout the drinking water supply chain in Haikou City, China, and to conduct risk assessments regarding the relationship between MPs contamination and human health. The results revealed that the abundance of MPs in raw, treated, and tap water was 0.

2D Cerium-Organic Frameworks as an Efficient Heterogeneous Catalyst for the Synthesis of 1,4-Dihydropyridines via Hantzsch Reaction.

Herein, a new two-dimensional (2D) Ce-organic frameworks (referred to as SLX-4) was achieved by traditional solvothermal conditions. Initial studies of SLX-4 toward Hantzsch reaction showed that good catalytic activity can be obtained under mild conditions, giving the desired 1,4-dihydropyridines in moderate to high yields. The catalyst could be reused at least 4 times keeping good catalytic activity.

Pentachlorophenol impairs the antimicrobic capability of blood clam via undermining humoral immunity and disrupting humoral-cellular crosstalk.

Due to past massive usage and persistent nature, pentachlorophenol (PCP) residues are prevalent in environments, posing a potential threat to various organisms such as sessile filter-feeding bivalves. Although humoral immunity and its crosstalk with cellular one are crucial for the maintaining of robust antimicrobic capability, little is known about the impacts of PCP on these critical processes in bivalve mollusks. In this study, pathogenic bacterial challenge and plasma antimicrobic capability assays were carried out to assess the toxic effects of PCP on the immunity of a common bivalve species, blood clam (Tegillarca granosa).

Synthesis of C(3) SCF-Substituted Pyrrolidinoindoline by P/P Redox Catalysis Using CFSOCl as Electrophilic CFS Reagent.

This work reports a method for the catalytic synthesis of C(3) SCF-substituted pyrrolidinindoline using a small-ring organophosphorus-based catalyst and a hydrosilane reductant, with trifluoromethanesulfonyl chloride as the electrophilic SCF reagent. This method can drive the conversion of tryptamine to the C(3) SCF-substituted pyrrolidine indoline. The readily available, inexpensive trifluoromethanesulfonyl chloride could be activated as an electrophilic SCF source by P/P redox catalysis and could efficiently participate in the reaction of tryptamines, thus providing various substituted C(3) SCF-substituted pyrrolidinoindoline in moderate to excellent yields.

Infection Inhibits the Efficacy of RBD Protein of SARS-CoV-2 Vaccination via Regulating Humoral and Cellular Immunity.

Vaccines are the most effective and feasible way to control pathogen infection. Helminths have been reported to jeopardize the protective immunity mounted by several vaccines. However, there are no experimental data about the effect of helminth infection on the effectiveness of COVID-19 vaccines.

Micro/nanoplastics impair the feeding of goldfish by disrupting the complicated peripheral and central regulation of appetite.

The ubiquitous presence of plastic particles in water bodies poses a potential threat to aquatic species. Although numerous adverse effects of microplastics (MPs) and nanoplastics (NPs) have been documented, their effects on fish feeding, one of the most important behaviors of animals, are far from being fully understood. In this study, the effects of MPs and NPs (at environmentally realistic levels) on fish food consumption and feeding behavior were assessed using goldfish (Carassius auratus) and polystyrene (PS) particles as representatives.

P/P-Catalyzed Beckmann Reaction and Sequential [2,3]-Sigmatropic Rearrangement to Construct 2-Amidopyridines.

An organophosphorus catalytic method for the synthesis of substituted 2-amidopyridines is reported. The method employs a small-ring organophosphorus-based catalyst and a hydrosilane reductant to drive the conversion of ketoximes and pyridine--oxides into 2-amidopyridines through sequential Beckmann rearrangement followed by [2,3]-sigmatropic rearrangement. The readily available ketoximes could be activated to nitrilium ions in P/P redox catalysis and could efficiently participate in the domino reaction of pyridine--oxides, thus providing various substituted 2-amidopyridines in moderate to excellent yields.

Enrofloxacin exposure undermines gut health and disrupts neurotransmitters along the microbiota-gut-brain axis in zebrafish.

The environmental prevalence of antibiotic residues poses a potential threat to gut health and may thereby disrupt brain function through the microbiota-gut-brain axis. However, little is currently known about the impacts of antibiotics on gut health and neurotransmitters along the microbiota-gut-brain axis in fish species. Taking enrofloxacin (ENR) as a representative, the impacts of antibiotic exposure on the gut structural integrity, intestinal microenvironment, and neurotransmitters along the microbiota-gut-brain axis were evaluated in zebrafish in this study.

Microplastics weaken the exoskeletal mechanical properties of Pacific whiteleg shrimp Litopenaeus vannamei.

The ubiquitous presence of microplastics (MPs) in aquatic environments poses a significant threat to crustaceans. Although exoskeleton quality is critical for crustacean survival, the impact of MPs on crustacean exoskeletons remains elusive. Our study represents a pioneering effort to characterize the effects of MPs exposure on crustacean exoskeletons.

Microplastics aggravate the bioaccumulation and corresponding food safety risk of antibiotics in edible bivalves by constraining detoxification-related processes.

Characterized by a sessile filter-feeding lifestyle, commercial marine bivalves inhabiting pollution-prone coastal areas may accumulate significant amounts of pollutants, such as antibiotic residues, in their soft tissues and thus pose a potential threat to the health of seafood consumers. Microplastics are another type of emerging pollutant that are prevalent in coastal areas and have been reported to interact with common antibiotics such as enrofloxacin (ENR) and trimethoprim (TMP). Nevertheless, little is known about the impacts of MPs on the accumulation and corresponding food safety risk of antibiotics in edible bivalve species.

A C-type lectin with a single carbohydrate-recognition domain (CRD) containing unique QPN/WDD motifs from Tegillarca granosa is involved in the innate immune defense.

C-type lectins (CTLs), a superfamily of Ca-dependent carbohydrate-recognition proteins, serve as pattern recognition receptors (PRRs) in the immune response of many species. However, little is currently known about the CTLs of the commercially and ecologically important bivalve species, blood clam (Tegillarca granosa). In this study, a CTL (designated as TgCTL-1) with a single carbohydrate-recognition domain (CRD) containing unique QPN/WDD motifs was identified in the blood clam through transcriptome and whole-genome searching.

Exposure to Polystyrene Nanoplastics Led to Learning and Memory Deficits in Zebrafish by Inducing Oxidative Damage and Aggravating Brain Aging.

Nanoplastics (NPs) may pass through the blood-brain barrier, giving rise to serious concerns about their potential toxicity to the brain. In this study, the effects of NPs exposure on learning and memory, the primary cognitive functions of the brain, are assessed in zebrafish with classic T-maze exploration tasks. Additionally, to reveal potential affecting mechanisms, the impacts of NPs exposure on brain aging, oxidative damage, energy provision, and the cell cycle are evaluated.

Toxic effects of four emerging pollutants on cardiac performance and associated physiological parameters of the thick-shell mussel (Mytilus coruscus).

Robust cardiac performance is critical for the health and even survival of an animal; however, it is sensitive to environmental stressors. At present, little is known about the cardiotoxicity of emerging pollutants to bivalve mollusks. Thus, in this study, the cardiotoxic effects of four emergent pollutants, carbamazepine (CBZ), bisphenol A (BPA), tetrabromobisphenol A (TBBPA), and tris(2-chloroethyl) phosphate (TCEP), on the thick-shell mussel, Mytilus coruscus, were evaluated by heartbeat monitoring and histological examinations.