Anxiety, depression and fear of disease progression among Chinese parents of children with retinoblastoma.

Background: Recent studies indicate parents of childhood cancer survivors experience high psychological burden. However, there is limited knowledge regarding the mental health of parents of children with retinoblastoma (RB) in China. Thus, the purpose of this study was to assess anxiety, depression, fear of disease progression (FoP), and family function among parents of children with RB, and to analyze associated factors.

Retracing the path of evolution: polymorphisms of codon 363 shape the fitness of .

, the etiologic agent of plague, is a genetically monomorphic pathogen. By screening 1085 published genomes, we identified an unusual mutation hotspot within codon 363 of the aspartate ammonia-lyase gene with at least six alleles in the population. Our investigation delves into the significance of the polymorphism at the codon 363 and its impact on the fitness of strains.

Molecular mechanisms and applications of natural transformation in bacteria.

Natural transformation is a process in which bacteria uptake exogenous DNA from the environment during a transient physiological state called competence. The DNA can either autonomously replicate or integrate into the bacterial chromosome through homologous recombination. Natural transformation has been studied for nearly a century.

FAS mediates apoptosis, inflammation, and treatment of pathogen infection.

The FAS cell surface death receptor, a member of the tumor necrosis factor receptor family, activates both apoptotic and non-apoptotic signaling upon interaction with its ligand FASL. It is critical in cell migration, invasion, immune responses, and carcinogenesis. Pathogen infection can influence host cells' behavior by modulating the FAS/FASL pathway, thereby influencing disease progression.

Micellar casein were constructed to improve the encapsulation efficiency of algae oil docosahexaenoic acid by transglutaminase-coupled phosphoserine peptide chelating with Ca.

Micelle systems using safe food-grade biopolymers are of particular interest for the encapsulation and delivery of nutrition components. Micellar casein (MC) was assembled using transglutaminase (TGase) to couple with phosphoserine peptide, which enhance the stability of docosahexaenoic acid (DHA) from algae oil. The mechanism behind the construction of MC-phosphoserine peptide and the encapsulation of DHA was explored.

Activation of glycolysis alleviates mitochondrial impairments caused by social isolation in Drosophila.

Social isolation (SI) in humans can lead to various psychological and physical abnormalities. However, the molecular mechanisms and potential drug treatments for this illness are not well understood. Drosophila, a social organism, exhibits distinct behavioral defects under SI conditions, such as reduced sleep and loss of sugar intake preference.

The Characteristic of Biofilm Formation in ESBL-Producing Isolates.

is a pathogen that commonly causes hospital-acquired infections. Bacterial biofilms are structured bacterial communities that adhere to the surface of objects or biological tissues. In this study, we investigated the genome homology and biofilm formation capacity of ESBL-producing .

Simulated spaceflight-induced cardiac remodeling is modulated by gut microbial-derived trimethylamine N-oxide.

Spaceflight is physically demanding and can negatively affect astronauts' health. It has been shown that the human gut microbiota and cardiac function are affected by spaceflight and simulated spaceflight. This study investigated the effects of the gut microbiota on simulated spaceflight-induced cardiac remodeling using 10° of head-down bed rest (HDBR) in rhesus macaques and 30° of hindlimb unloading (HU) in mice.

Characterization of an aspartate aminotransferase encoded by YPO0623 with frequent nonsense mutations in .

, the causative agent of plague, is a genetically monomorphic bacterial pathogen that evolved from approximately 7,400 years ago. We observed unusually frequent mutations in YPO0623, mostly resulting in protein translation termination, which implies a strong natural selection. These mutations were found in all phylogenetic lineages of , and there was no apparent pattern in the spatial distribution of the mutant strains.

and Plague: some knowns and unknowns.

Since its first identification in 1894 during the third pandemic in Hong Kong, there has been significant progress of understanding the lifestyle of , the pathogen that is responsible for plague. Although we now have some understanding of the pathogen's physiology, genetics, genomics, evolution, gene regulation, pathogenesis and immunity, there are many unknown aspects of the pathogen and its disease development. Here, we focus on some of the knowns and unknowns relating to and plague.

Body weight, weight change and the risk of cardiovascular disease in patients with hypertension: a primary-care cohort study.

Background/objectives: Obesity and cardiovascular disease (CVD) often co-occur. However, the effects of excessive body weight and weight change on CVD in patients with hypertension are not clearly established. We examined the associations of BMI, weight change and the risk of CVD in patients with hypertension.

[Clinical feature and genetic analysis of a patient with Idiopathic hypogonadotropic hypogonadism due to a novel variant of CHD7 gene].

Objective: To explore the clinical feature and genetic etiology of a patient with normosmic idiopathic hypogonadotropic hypogonadism (nIHH) due to variant of CHD7 gene.

Methods: A patient who had presented at Anhui Provincial Children's Hospital in October 2022 was selected as the study subject. Clinical data of the patient was collected.

Preparation of polyclonal antibody against a universal bacterial antigen OmpA deduced by bioinformatic analysis and preliminary evaluation of concentration effects on foodborne pathogens.

Rapid and ultrasensitive microbial detection in actual samples have challenges because of target pathogen diversity and low abundance. In this study, we attempted to capture and concentrate multiple pathogens by combining magnetic beads with polyclonal antibodies against a universal antigen of , LAMOA-1, before further detection. A protein sequence consisting of 241 amino acids with spatial conformation similar to was identified and expressed as a recombinant protein in prokaryotes according to the results of sequence alignment among 432 sequences of belonging to intestinal bacteria from gram-negative bacteria.

Effects of 60 days of 6° head-down bed rest on the composition and function of the human gut microbiota.

Spaceflight is rigorous and dangerous environment which can negatively affect astronauts' health and the entire mission. The 60 days of 6° head-down bed rest (HDBR) experiment provided us with an opportunity to trace the change of gut microbiota under simulated microgravity. The gut microbiota of volunteers was analyzed and characterized by 16S rRNA gene sequencing and metagenomic sequencing.

Vimar/RAP1GDS1 promotes acceleration of brain aging after flies and mice reach middle age.

Brain aging may accelerate after rodents reach middle age. However, the endogenous mediator that promotes this acceleration is unknown. We predict that the mediator may be expressed after an organism reaches middle age and dysregulates mitochondrial function.

The impact of COVID lockdown on glycaemic control in paediatric patients with type 1 diabetes: A systematic review and meta-analysis of 22 observational studies.

Introduction: The COVID lockdown has posted a great challenge to paediatric patients with type 1 diabetes (T1D) and their caregivers on the disease management. This systematic review and meta-analysis sought to compare the glycaemic control among paediatric patients with T1D (aged under 18 years) pre- during, and post-lockdown period.

Methods And Materials: We did a systematic search of three databases (PubMed, Embase, and the WHO COVID-19 Global literature) for the literature published between 1 Jan 2019 to 10 Sep 2022.

Comparison of the Efficacy of Different Insulin Administration and Blood Glucose Monitoring Methods in the Treatment of Type 1 Diabetes Mellitus in Children.

Objective: To compare the clinical efficacy of different insulin administration methods and blood glucose monitoring methods in treating type 1 diabetes mellitus in children.

Methods: Patients were divided into four groups: multiple daily injection (MDI) + fingertip blood glucose detection, continuous subcutaneous insulin infusion (CSII) + fingertip blood glucose detection, MDI + continuous glucose monitoring system (CGMS), and CSII + CGMS. After six months of treatment, followed by telephone and at least once a month in an outpatient clinic, insulin doses were adjusted according to the children's blood glucose levels.

The Risks of Cardiovascular Disease Following Weight Change in Adults with Diabetes: A Cohort Study and Meta-analysis (jc.2022-01089).

Context: Weight management is recognized as critical in reducing cardio-metabolic risk factors for adults with diabetes, but the effects of weight change on cardiovascular disease in patients with diabetes are unknown.

Objective: To evaluate 18-month weight change and subsequent risk of macrovascular and microvascular complications in established individuals with type 2 diabetes.

Design And Setting: This study consisted of a cohort study and a meta-analysis.

Changes in Higher-Order Chromosomal Structure of Under Simulated Microgravity.

Our previous work have shown that certain subpopulations of exhibit significant phenotypic changes under simulated microgravity (SMG), including enhanced biofilm formation and cellulose synthesis, which may be evoked by changes in gene expression patterns. It is well known that prokaryotic cells genomic DNA can be hierarchically organized into different higher-order three-dimensional structures, which can highly influence gene expression. It is remain elusive whether phenotypic changes induced by SMG in the subpopulations of are driven by genome higher-order structural changes.

Attenuation of Mutants Caused by Iron Uptake Inhibition and Decreased Survivability in Macrophages.

is the etiological agent of plague, a deadly infectious disease that has caused millions of deaths throughout history. Obtaining iron from the host is very important for bacterial pathogenicity possesses many iron uptake systems. Yersiniabactin (Ybt) plays a major role in iron uptake and , and in virulence toward mice as well.

PgtE Enzyme of Shares the Similar Biological Roles to Plasminogen Activator (Pla) in Interacting With DEC-205 (CD205), and Enhancing Host Dissemination and Infectivity by .

, the cause of plague, is a newly evolved Gram-negative bacterium. Through the acquisition of the plasminogen activator (Pla), gained the means to rapidly disseminate throughout its mammalian hosts. It was suggested that utilizes Pla to interact with the DEC-205 (CD205) receptor on antigen-presenting cells (APCs) to initiate host dissemination and infection.

miR-126-5p Targets SP1 to Inhibit the Progression of Parkinson's Disease.

Background: At present, symptomatic treatment may improve the life quality of Parkinson's disease (PD) patients to a certain extent but cannot completely cure PD. Therefore, it is urgent medical problem to be solved for improving the efficacy and safety of PD treatment.

Methods: SH-SY5Y and SK-N-SH cells were treated with 1-methyl-4-phenylpyridinium (MPP+) to establish PD model cells.

[Clinical characteristics and genetic analysis of an ethnic Han Chinese child with Keppen-Lubinsky syndrome due to a de novo KCNJ6 mutation].

Objective: To investigate the clinical characteristics and genetic basis for a child with Keppen-Lubinsky syndrome (KPLBS).

Methods: Trio-whole exome sequencing (Trio-WES) was carried out for the proband and her parents. Candidate variant was verified by Sanger sequencing and bioinformatic analysis.