Chromosomal-level genome assembly of an allotetraploid oyster.

Tetraploid oysters are used to cross with diploids to produce triploid oysters that have become an important part of the oyster aquaculture industry worldwide. Although most tetraploid oysters are artificially induced autotetraploids, allotetraploids can be produced between closely related species, providing new opportunities for polyploid breeding and studying genome interactions. Using PacBio sequencing, Illumina sequencing, and high-throughput chromosome conformation capture scaffolding, we produced a high-quality genome assembly of an artificially induced allotetraploid between the Pacific oyster Crassostrea gigas and Portuguese oyster Crassostrea angulata.

Schistosoma japonicum leishmanolysin SjLLPi1 facilitates the invasion of cercariae into the host skin.

Background: Schistosomiasis is an important neglected tropical disease necessitating focus. Cercarial proteases are essential for schistosome invasion. Leishmanolysin has been identified as the most predominant protease in Schistosoma japonicum (S.

Design, synthesis, and biological evaluation of Pseudolaric acid B derivatives as Ferroptosis inducers in Colon Cancer cells.

The inhibition of glutathione peroxidase 4 (GPX4) is an effective strategy to suppress the proliferation of tumor cells by inducing ferroptosis. To obtain GPX4 inhibitors, we designed and synthesized 34 pseudolaric acid B (PAB) derivatives. The results of the cytotoxicity test showed that compound 30 significantly inhibited the proliferation of colon cancer cells (HCT-116 and HT-29).

Short-term atmospheric turbulence prediction in a satellite-to-ground laser link with limited observed data.

Satellite-to-ground laser communications can download massive amounts of data from satellites, thus playing an important role in space science. However, the performance of data downloading will decrease severely if there is stronger atmospheric turbulence (AT) in the link. Therefore, predicting AT strength in advance is crucial, as it will provide efficient strategies for data transmission and for following adaptive optical systems.

Photonic crystal-based structural color switches.

Photonic crystals (PCs), a class of advanced optical metamaterials, have garnered considerable attention in the scientific community due to their versatile structural configurations and precisely tunable photonic stop band (PSB) properties. Unlike traditional stimuli-responsive PCs, PC-based structural color switches exhibit exceptional capabilities in modulating between colored and non-colored states in response to external stimuli, offering significant potential for advanced optical applications. Despite growing interest in this field, systematic reviews comprehensively addressing the fundamental mechanisms governing structural color switching and their practical implementation remain scarce.

[Mechanisms and treatment of inflammation-cancer transformation in colon from perspective of cold and heat in complexity in integrative medicine].

Colorectal cancer(CRC) is one of the most common malignant tumors worldwide, primarily originating from recurrent inflammatory bowel disease(IBD). Therefore, blocking the inflammation-cancer transformation in the colon has become a focus in the early prevention and treatment of CRC. The inflammation-cancer transformation in the colon involves multiple types of cells and complex pathological processes, including inflammatory responses and tumorigenesis.

Defining Economic and Environmental Typologies across 77 Countries to Prioritize Opportunities for Nonsewered Sanitation.

Lack of access to sanitation is a challenge that persists globally, with low sewerage connection rates in many low- and lower-middle-income countries. Engineered nonsewered sanitation (NSS) technologies can meet treatment requirements without sewers, but their relative sustainability varies across potential deployment sites. Here, we characterize the costs and carbon intensity (CI) of three emerging NSS technologies, two community reinvented toilets (CRTs) and one Omni Processor (OP), across 77 countries, identify sustainability performance typologies, and map typology prevalence in countries across the globe.

Study on the willingness to enroll in the urban and rural residents basic medical insurance: from the perspectives of policy awareness and institutional trust.

Background: Achieving universal health coverage remains a global health priority. Understanding factors that influence individuals' willingness to enroll in basic medical insurance is essential for system sustainability.

Objectives: This study aims to examine the impact of policy awareness and institutional trust on the willingness of urban and rural residents to enroll in the Urban and Rural Residents Basic Medical Insurance (URRBMI), with implications for policy refinement.

Effect of Adding Alkaline Metal Ions Complexes Rumen Microbiota and Metabolome of Hu Lambs.

This study aimed to evaluate the effects of studying the effects of the alkali metal ion complexes (AMIC) on the rumen of lambs. Eighteen 3-month-old male Hu lambs (30 ± 2.5 kg) were randomly assigned to three groups ( = 6).

Enhanced Quality in Bean Products Through Mixed Fermentation: A Comparative Analysis of Physicochemical, Structural, and Functional Properties of Soybean Products.

This study investigated the quality evolution of soybean products (soymilk, tofu, dried bean curd) through mixed-strain fermentation with and under optimized conditions (7% inoculum, pH of 5.2, 85 °C/50 min thermal treatment). Physicochemical, structural, and microbial dynamics were systematically analyzed.

Oligogalacturonides Operate as Endogenous Elicitors to Regulate Aluminum Tolerance in Pea (Pisum sativum).

Aluminum (Al) toxicity is a major limiting factor leading to crop yield reduction in acidic soils. The pectic polysaccharides, key components of plant cell walls, are considered the primary binding site for Al ions. Oligogalacturonide (OGA), the oligomers of alpha-1,4-linked galacturonosyl residues originating from the degradation of cell wall pectin (homogalacturonan), are able to elicit defense responses and protect plants against biotic stress, such as pathogen infections.

Efficacy of a postbiotic and its components in promoting colonic transit and alleviating chronic constipation in humans and mice.

This study evaluates the efficacy of the postbiotic Probio-Eco in alleviating constipation in humans and mice. A randomized, double-blind, placebo-controlled crossover trial involving 110 adults with chronic constipation (Rome IV criteria) demonstrates that a 3-week Probio-Eco intervention significantly improves constipation symptoms, stool straining, and worry scores. Gut microbiota and metabolomic analyses reveal modulations in specific gut microbiota, succinate, tryptophan derivatives, deoxycholate, propionate, butyrate, and cortisol, correlating with symptom relief.

A novel liver-targeted and highly selective fluorescent probe for hepatic hydrogen sulfide detection in the diagnosis of drug-induced liver injury.

Drug-induced liver injury (DILI) has emerged as a significant health risk, yet reliable methods of detection at an early stage are in urgent need. Recent researches have highlighted the crucial role of hydrogen sulfide (HS) in various pathological and physiological processes, with a correlation between its levels and the severity of DILI. We developed a novel fluorescent probe HS-YL for the precise detection of liver HS fluctuations in DILI, and the liver in situ accumulation capacity of HS-YL has been significantly enhanced by incorporating a liver-targeting cholic acid moiety into its skeleton.

Identification of low-toxicity DNA topoisomerase I inhibitors with potential blood-brain barrier penetrability for glioblastoma therapy: structure-based virtual screening reveals promising novel Scaffolds.

Due to the blood-brain barrier (BBB), DNA topoisomerase I (Topo I) inhibitors often cause dose-limiting toxicity in glioblastoma (GBM) treatment. Therefore, developing low-toxicity Topo I inhibitors with enhanced BBB permeability holds a significant promise for improving GBM treatment outcomes. In this study, structure-based virtual screening methods combined with biological evaluations successfully identified three potent Topo I inhibitors, which exhibited IC values of approximately 25 µM against A172 cells.

Sanshen San Formula Hinders Cognitive Function and Pathology in Alzheimer's Disease Through Potentiating the Function of Synapse.

Background: Alzheimer's disease (AD) constitutes a devastating neurodegenerative disorder, manifested by amyloid-β aggregation, phosphorylated tau accumulation, and progressive cognitive deterioration. Current therapeutic interventions remain predominantly symptomatic, underscoring the urgency for more efficacious treatment strategies.

Purpose: This study elucidated the therapeutic potential of Sanshen San (SSS), a traditional Chinese herbal formula encompassing Polygala Radix, Pini Radix in Poria, and Acori Tatarinowii Rhizoma, on cognitive function and AD pathology.

Prediction of Breast Cancer Remission.

Background And Objectives: This study aims to use electronic health records (EHR) and social determinants of health (SDOH) data to predict breast cancer remission. The emphasis is placed on utilizing easily accessible information to improve predictive models, facilitate the early detection of high-risk patients, and facilitate targeted interventions and personalized care strategies.

Methods: This study identifies individuals who are unlikely to respond to standard treatment of breast cancer.

High-salt diet drives depression-like behavior in mice by inducing IL-17A production.

Major depression disorder is a common illness that severely limits psychosocial functioning, affects outcomes of other diseases, and diminishes the quality of life. High-salt diet (HSD) has long been closely associated with the occurrence and development of depression, but whether or how HSD causes depression remains unclear. In this study, HSD induces depression-like behavior in mice accompanied by an increase in IL-17A production.

Spinal KCC2 Mediates the Modulation Effect of HDAC2 on Bone Cancer Pain in Rats.

Background: Bone cancer pain is a global medical concern with limited treatment options that significantly reduce the quality of life for cancer patients. Therefore, identifying a promising therapeutic target for bone cancer pain is urgently needed.

Objective: Our previous research indicated that KCC2 may be associated with the modulation of HDAC2 in a rat model of bone cancer pain.

Research on Factors Affecting Asphalt Mixtures' Resistance to High-Frequency Freeze-Thaw in Plateau Areas.

Aiming at the problem that asphalt pavement materials in plateau areas are vulnerable to freeze-thaw damage, research was carried out on asphalt pavements of representative road sections, and the temperature within the pavement structure was monitored using buried sensors. Based on this, an indoor test method for high-frequency freeze-thaw was established, and UV, thermo-oxygen-aging and high-frequency freeze-thaw tests were combined. The effects of aging and maximum aggregate particle size on the resistance of asphalt mixtures to high-frequency freeze-thaw were investigated using the splitting strength ratio, mass-loss rate and void-ratio changes by employing the newly made RS-type modified asphalt in the laboratory.

L-histidine makes Ni 'visible' for plant signalling systems: Shading the light on Ni-induced Ca and redox signalling in plants.

Nickel is both an important nutrient and an ecotoxicant for plants. Organic ligands, such as L-histidine (His), play a key role in Ni detoxification. Here, we show that His (added together with 0.

Boron controls apical dominance in Pea (Pisum sativum) via promoting polar auxin transport.

Plant architecture and subsequent productivity are determined by the shoot apical dominance, which is disturbed by the deficiency of boron, one of the essential trace elements for plant growth and reproduction. However, the mechanism by which B controls shoot apical dominance or axillary bud outgrows under B deficiency is still unclear. This work aimed to investigate the mechanistic basis of this process, with focus on the interaction between B and polar auxin transport.

Deep learning-based prediction of atmospheric turbulence toward satellite-to-ground laser communication.

Atmospheric turbulence is one of the key factors that affect the stability and performance of satellite-to-ground laser communication (SGLC). Predicting turbulence could provide a decisive strategy for the SGLC system to ensure communication performance and is thus of great significance. In this Letter, we proposed a hybrid multi-step prediction method for atmospheric turbulence.

[Construction of prognostic prediction model for patients with sepsis-induced acute kidney injury treated with continuous renal replacement therapy].

Objective: To explore the influencing factors of prognosis in patients with sepsis-induced acute kidney injury undergoing continuous renal replacement therapy (CRRT), and to construct a mortality risk prediction model.

Methods: A retrospective research method was adopted, patients with sepsis-induced acute kidney injury who received CRRT at Fuyang People's Hospital from February 2021 to September 2023 were included in this study. Collect general information, comorbidities, vital signs, laboratory indicators, disease severity scores, treatment status, length of stay in the intensive care unit (ICU), and 28-day prognosis were collected within 24 hours of patient enrollment.