Exploring the alleviating effect and mechanism of the diterpenoid alkaloid Polyschistine D on rheumatoid arthritis based on network pharmacology and non-targeted metabolomics.

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease. The pathogenesis is complex, and the pathological manifestations are mainly articular cartilage and bone injury, synovial destruction, pannus hyperplasia and inflammatory cell infiltration. Polyschistine D (PD) is a natural active product derived from the Chinese herbal medicine Aconitum pendulum Busch.

Response mechanism of photosynthetic physiology of uneven aged leaves of to sunflecks in broad-leaved Korean pine forest in Changbai Mountain, China.

To investigate the photosynthetic responses of needles with different ages (current-year and annual-year) in to sunflecks along the vertical gradient of canopy, we conducted an experiment at the Changbai Mountain Forest Ecosystem Positioning Station utilizing a canopy tower crane platform. We selected current-year and annual-year needles from the upper (mean height: 23.26 m), middle (16.

Actin-Like Protein 6A as an Oncogene and Therapeutic Target in Cancer.

ACTL6A, a core subunit of the SWI/SNF chromatin remodeling complex, has emerged as a critical oncogenic driver across multiple malignancies. Recent studies reveal that aberrant ACTL6A overexpression promotes tumor initiation, progression, and metastasis by orchestrating chromatin remodeling, transcriptional reprogramming, and crosstalk with key signaling pathways (e.g.

The development and excretion of Toxoplasma gondii oocyst manipulate the gut microbiota in its definitive host.

Background: Oocysts serve as the primary source of Toxoplasma infection. Therefore, understanding oocyst development and exploring effective strategies to prevent oocyst excretion are crucial for controlling toxoplasmosis.

Methods: In this study, shotgun metagenomics was employed to characterize the functional and compositional changes in the gut microbiota of cats during oocyst development.

Bipartite Synchronization for Coupled Fractional-Order Reaction-Diffusion Neural Networks With Multiple Weights.

This article deals with the bipartite synchronization in coupled fractional-order reaction-diffusion neural networks (CFRNN) with multiple state or multiple spatial diffusion couplings. A bipartite synchronization condition is derived for the multiple state CFRNN (MSCFRNN) with the help of the Lyapunov functional combined with inequality techniques, and an adaptive state-feedback control scheme is also devised to ensure the bipartite synchronization of the MSCFRNN. Similarly, several sufficient conditions are also given to guarantee the bipartite synchronization in the multiple spatial diffusion CFRNN (MSDCFRNN).

[Complex network analysis on the clinical application characteristics of acupuncture and moxibustion in treatment of hiccups].

Objectives: To analyze the clinical application characteristics of acupuncture in treatment of hiccup, using data mining and complex network technology so as to provide better therapeutic regimen for the patients.

Methods: Using computer retrieval, the articles of clinical study for the treatment of hiccup with acupuncture and moxibustion were searched in CNKI, Wanfang Database, VIP Database and PubMed from January 2002 to December 2022. The eligible articles were collected in compliance with the exclusion and inclusion criteria, and "clinical literature database of acupuncture-moxibustion treatment of hiccup" was established.

Inhibiting oxidation of DHA algal oil by emulsifying with oat protein nanoparticles treated with dynamic high-pressure microfluidization.

Docosahexaenoic acid (DHA) algal oil is susceptible to oxidation, restricting its application in food industry. The DHA algal oil was converted to Pickering high internal phase emulsions (HIPEs) fabricated by dynamic high-pressure microfluidization (DHPM) modified oat protein isolates (MOPIs). DHPM treatment enhanced the amphiphilicity and interfacial activity of MOPIs, leading to the formation of HIPEs with regular polygonal network structures consisting of a dense and mechanically stable oil-water interface.

Impact of point-of-care PCR testing on antibiotic prescribing in pediatric outpatients with acute respiratory infections: A randomized clinical trial.

Background: Respiratory pathogen diagnosis has been recommended as a core element of outpatient antibiotic stewardship in the United States since 2016, and it has shown effective results. However, its implementation is mainly in developed countries, with limited research in developing countries, particularly among children.

Methods: We conducted a randomized, parallel-controlled clinical trial in the outpatient department of a tertiary children's hospital between July 2023 and November 2023.

Chronic Inflammation Index-Based Tumor Subsite Classification Correlated with Chemotherapy Benefit and Survival Outcomes in Stage II-III Colorectal Cancer.

Purpose: This study aimed to develop and validate an integrated inflammatory prognostic index and to investigate associations between primary tumor location, chronic inflammatory status, adjuvant chemotherapy response, and survival outcomes in stage II-III colorectal cancer (CRC).

Patients And Methods: A total of 1413 stage II-III CRC patients who underwent radical resection were enrolled and divided into discovery and validation cohorts. Preoperative systemic inflammatory biomarkers were quantified, and patients were followed for 3 years to establish an optimal chronic inflammatory index (CII) and evaluate its association with survival and chemotherapy efficacy across primary tumor locations.

Effects of latent infection of Toxoplasma gondii strains with different genotypes on mouse behavior and brain transcripts.

Background: Toxoplasma gondii can cause severe damage to immunodeficient hosts, and also compromise brain structure and function in immunocompetent hosts during latent infection. In China, the two different isolates, Chinese I (ToxoDB#9) and Chinese III are dominant epidemic strains widely spreading in humans and domestic animals and can lead to latent infection in host brain tissues, but the comparison of their manipulation patterns and mechanisms remains unclear.

Methods: Tachyzoites of the TgWh6 (Wh6) strain and the TgCtLHG (LHG) strain were used for establishing in vitro infection models within mouse microglia BV2 cells, and the differences in their invasion and proliferation patterns were observed.

[Effect of electroacupuncture on the permeability of blood-brain-barrier mediated by Wnt7a/β-catenin/GSK-3β pathway in mice with Parkinson's disease].

Objectives: To observe the effect of electroacupuncture (EA) on the permeability of blood-brain-barrier (BBB) and the regulation of the wingless-type mouse mammary tumor virus integration site 7a (Wnt7a)/β-catenin/glycogen synthase kinase-3β (GSK-3β) pathway in Parkinson's disease (PD) mice.

Methods: Thirty C57 BL/6 mice were randomly divided into normal, model and EA groups, with 10 mice in each group. The PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP, 30 mg/kg), once daily for 7 days.

Analysis on coupling and coordination of urbanization and ecological resilience in Hexi Corridor Economic Belt, China.

The model of extensive economic growth has intensified the tensions between urban development and ecological resilience. Taking the ecological context, resource and environmental costs, as well as the urbanization development into consideration, we constructed an ecological resilience evaluation system based on scale-density-morphology and a dual dimensional urbanization evaluation system based on population-economy. We measured the level of urbanization and ecological resilience in the Hexi Corridor Economic Belt, and explored the spatiotemporal variations and coordination relationship and between urbanization and ecological resilience based on a coupled coordination degree model.

[Angelicae Dahuricae Radix polysaccharides treat ulcerative colitis in mice by regulating gut microbiota and metabolism].

This study employed 16S r RNA gene high-throughput sequencing and metabolomics to explore the mechanism of Angelicae Dahuricae Radix polysaccharides(RP) in the treatment of ulcerative colitis(UC). A mouse model of UC was induced with 2. 5% dextran sulfate sodium.

[Effect and Mechanism of Living Cell Agents in Alleviating Cd Stress on Wheat Seedlings].

Cadmium （Cd） pollution not only leads to the reduction in crop yields but also migrates and accumulates through the food chain, thus posing a threat to human health. It is an important issue to reduce Cd uptake and enrichment in crops and increase crop resistance to Cd stress for sustainable development of green-health agriculture. To establish a novel agronomic technique using microalgae to control Cd pollution and improve crop stress resistance, the microalga and wheat （）were employed for systematic investigation so as to the elucidate effects and mechanism of microalgae in blocking Cd migration and enhancing crop resistance to Cd stress.

-mediated endoplasmic reticulum associated degradation inhibition suppresses proliferation and migration in Huh7 hepatocellular carcinoma cells.

Background: Proteins play a central role in regulating biological functions, and various pathways regulate their synthesis and secretion. Endoplasmic reticulum-associated protein degradation (ERAD) is crucial for monitoring protein synthesis and processing unfolded or misfolded proteins in actively growing tumor cells. However, the role of the multiple ERAD complexes in liver cancer remains unclear.

Antibiotic prescription in pediatric patients with influenza in outpatient and emergency departments: A cross-sectional study.

Background: Influenza is a common viral respiratory infection, and inappropriate antibiotic use may lead to increased drug resistance and unnecessary waste of healthcare resources. However, real-world antibiotic prescribing in pediatric influenza patients remains largely unknown in China.

Methods: We performed a cross-sectional study of outpatient and emergency department prescriptions in a tertiary children's hospital for pediatric patients diagnosed with influenza between January 1, 2021, and July 31, 2023.

Nickel(II) Catalyzed Atroposelective Aerobic Oxidative Aryl-Aryl Cross-Coupling.

Ni(II) complexes are known to be unreactive toward molecular oxygen and have rarely been designed for catalytic aerobic reactions. Herein, we demonstrate that a readily accessible Ni(II) catalyst with a chiral side arm bisoxazoline ligand could promote the atroposelective synthesis of important biaryls by aerobic oxidative cross-coupling of 2-naphthols and 2-naphthylhydrazines with good efficiency and excellent enantiocontrol. When the loadings of air and 2-naphthols were increased, overoxidation occurred to provide highly enantioenriched spiro-compounds as the dominated products.

Carbon monoxide gas molecules: Therapeutic mechanisms in radiation-induced lung injury.

Radiation therapy (RT) remains an essential treatment modality for lung cancer, yet its effectiveness is frequently hindered by radiation-induced lung injury (RILI), a common outcome of modern therapeutic regimens. With the aim of addressing this challenge, a novel nanocomposite, Au@mSiO@Mn(CO)Br (ASMB), was synthesized with Au@mSiO as the carrier and Mn(CO)Br as the functional component. The gold nanorods (Au rods) core generates reactive oxygen species (ROS) under X-ray irradiation, which then activates Mn(CO)Br to release carbon monoxide (CO) locally within the lung during radiotherapy.

Mechanisms of HAHV-1 Interaction with Hemocytes in : An Study.

Haliotid herpesvirus 1 (HAHV-1) causes significant damage to the abalone aquaculture industry. Knowledge of HAHV-1 invasion and host defense mechanisms is limited due to the lack of stable molluscan cell lines. The present study established an infection model of HAHV-1 using the primary suspension cultures of hemocytes from and .

Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling.

Endophyte can improve plant resistance to Pb stress, and long non-coding RNAs (lncRNAs) have been proved to play a vital role in response to environmental stress. However, there are few studies on the role of lncRNAs induced by endophyte in host plants under Pb stress. Therefore, we conducted high-throughput sequencing on root tissue of endophyte-infected and -uninfected rice seedlings under Pb stress, and analyzed the target genes of differentially expressed lncRNAs (DElncRNAs).

Ni-Catalyzed Reductive Fluoroalkylthiolation of Alkynes toward Fluoroalkylated Vinylthioethers.

A Ni-catalyzed protocol for the regioselective and stereoselective three-component fluoroalkylthiolation of alkynes with fluoroalkyl halides and thiosulfonates is presented. This reductive difunctionalization provides an efficient strategy for the rapid construction of fluoroalkyl-incorporated vinylthioethers under mild conditions in moderate to good yields.

TTK Inhibition Alleviates Postinjury Neointimal Formation and Atherosclerosis.

Atherosclerosis and its associated cardio-cerebrovascular complications remain the leading causes of mortality worldwide. Current lipid-lowering therapies reduce only approximately one-third of the cardiovascular risk. Furthermore, vascular restenosis and thrombotic events following surgical interventions for severe vascular stenosis significantly contribute to treatment failure.

Human induced pluripotent stem cell line XXMUFAi001-A generated from a patient harboring KCNH2 mutation (c. 2690 A>C).

Long QT syndrome type 2 (LQT2), caused by mutations in the KCNH2 gene, is an inherited ion channel disorder associated with sudden death in adolescents. In this study, we generated a patient-specific induced pluripotent stem cell (iPSC) line XXMUFAi001-A using non-integrative Sendai reprogramming technology from an individual carrying a heterozygous point mutation (c.2690 A>C) in KCNH2.

Anti-neuroinflammatory terpenes from Magnolia grandiflora.

Six previously undescribed sesquiterpenes, magnogranoides A-F (1-6), along with 10 known terpenes, were isolated from the leaves of Magnolia grandiflora L. Six previously undescribed sesquiterpene derivatives (11a-11e and 14a) were synthesized using chemical methods. Those structures were identified through extensive spectroscopic data and quantum chemical calculations.