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Docosahexaenoic acid (DHA) algal oil is susceptible to oxidation, restricting its application in food industry. The DHA algal oil was converted to Pickering high internal phase emulsions (HIPEs) fabricated by dynamic high-pressure microfluidization (DHPM) modified oat protein isolates (MOPIs). DHPM treatment enhanced the amphiphilicity and interfacial activity of MOPIs, leading to the formation of HIPEs with regular polygonal network structures consisting of a dense and mechanically stable oil-water interface. Under the optimal conditions of an MOPIs concentration of 4 % (w/v), and DHA oil phase volume proportion of 0.80 (v/v), the HIPEs demonstrated favorable stability against storage, heat treatment, and oxidation. Over an 18-d storage period, the oxidation rate of DHA algal oil was reduced by more than 50.53 %. This work provides a clean-label, plant-based strategy to improve the oxidative stability of DHA algal oil and expands the application potential of oat proteins in functional foods and sustainable lipid delivery systems.
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http://dx.doi.org/10.1016/j.foodchem.2025.145072 | DOI Listing |
Biotechnol J
September 2025
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Schizochytrium sp., a marine alga prized for docosahexaenoic acid (DHA), was subjected to UV mutagenesis to boost industrial yields. The stable mutant UV1-3 achieved 5.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Department of Food Process Engineering, School of Bioengineering, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu 603203, India. Electronic address:
Docosahexaenoic acid (DHA) is an omega-3 fatty acid beneficial for brain development and cardiovascular health. DHA has poor aqueous solubility and is highly susceptible to oxidation, limiting its bioavailability. To address these challenges, in the present investigation, nanoemulsion was prepared using DHA-enriched edible algal oil and tween 80 as an emulsifier (1 %) initially.
View Article and Find Full Text PDFDiabetes Metab Syndr Obes
August 2025
Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.
Objective: To answer the question what is the best source or composition of omega-3 polyunsaturated fatty acids (PUFA) that will provide the most favorable and safe outcome for peripheral neuropathy (PN) in an animal model of obesity? Traditionally encapsulated fish oil is the primary source of omega-3 PUFA as a nutritional supplement. However, other sources exist that could be a better environmental, safety, and/or economic choice.
Methods: Male Sprague Dawley rats 12 weeks of age were fed a 45% kcal diet to induce obesity and model pre-diabetes.
Food Chem
August 2025
State Key Lab of Food Science and Resources, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address:
Sn-2 DHA-enriched triacylglycerols (TAG) were prepared by both lipase-catalyzed esterification and solvent fractionation. The specific-structured triacylglycerol (SST) was synthesized at a molar ratio of algae oil to mixed oleic acid and linoleic acid (3:2, w/w) of 1:5 with 8 % of Lipozyme TL IM at 60 °C for 8 h. In the SSTs, the DHA content reached 46 % with sn-2 DHA/total DHA of 50 %.
View Article and Find Full Text PDFJ Oleo Sci
August 2025
Department of Food Science and Technology, Tokyo University of Marine Science and Technology.
The oxidative stability of polyunsaturated fatty acid (PUFA) generally decreases with increasing unsaturation, however, the opposite results have been reported for aqueous emulsions prepared from PUFA. One study showed that among PUFA (fish oil) emulsions with varying μm-sized particles, the smaller emulsions had better oxidation stability. Thus, particle size may be involved in the mechanism behind the improved oxidative stability of PUFA by emulsification, and PUFA emulsions with even smaller particle sizes (i.
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