Pyomelanin-powered whole-cell biosensor for ultrasensitive and selective detection of bioavailable Hg(ii).

Rapid, low-cost trace inorganic Hg(ii) detection in environmental waters remains a critical public-health challenge. Here, we engineered into a naked-eye whole-cell biosensor by coupling a redesigned MerR-P element to the pyomelanin biosynthetic pathway. Three 4-hydroxyphenylpyruvate dioxygenase (HppD) homologs from WS, 4AK4, and PAO1 were codon-optimized and functionally screened.

Soft Sensing for Time Series With Irregular Sampling Internals Based on a Denoising Interval Attention LSTM Network.

The prediction of key quality variables plays an important role in industrial status identification and monitoring. Due to process disturbance and hard device limitation, data collection in modern industries often exhibits high noise and irregular data sampling. To solve the above problems, this article proposes a stacked supervised and reconstructed input denoising autoencoder integrated with internal attention long short-term memory (SSRDAE-IALSTM) network for soft sensing modeling.

Signal mining and risk analysis of tisotumab vedotin adverse events based on the FAERS database.

Tisotumab vedotin (TV), the first antibody-drug conjugate (ADC) targeting tissue factor, was granted approval by the Food and Drug Administration (FDA) for the treatment of recurrent or metastatic cervical cancer. However, its adverse events (AE) are mainly recorded in clinical trials, lacking of real-world data. The FDA Adverse Event Reporting System database was searched retrospectively, and the TV-related AE reports from July 2021 to September 2024 were extracted.

NXF1 suppresses progression of endometrial cancer by interacting with the SRSF3 to regulate SP4 splicing.

Conventional treatments for advanced endometriosis often have limited efficacy due to chemotherapy resistance, recurrence, and metastasis. This study analyzed clinical specimens to investigate the role of NXF1 in endometrial cancer (ECa) progression. Mouse models and molecular biology assays were used to elucidate NXF1's function and mechanisms and .

Optimizing chromosome dispersion quality: the key role of cell density.

Objective: This study aims to optimize metaphase dispersion in automated detection by quantitatively determining the optimal cell suspension density to enhance the accuracy and efficiency of chromosomal aberrations analysis.

Methods: Lymphocyte metaphase suspensions were prepared using an automated harvesting system and subjected to a concentration gradient of 10-10 cells/mL. Metaphase images were captured using an automated chromosome scanning and analysis system, and cell density, suspension turbidity, metaphase counts, and dispersion area were measured to quantitatively assess the impact of cell density on metaphase dispersion quality.

Exploration of current situation of psychotropic drugs research and development in China based on drug clinical trials.

Objective: To understand the current status of research and development (R&D) of psychotropic drugs.

Methods: Retrieved psychotropic drugs clinical trials (PDCTs) registered in China from 2019 to 2024 using the platform of , and systematically analyzed the data.

Results: Included four perspectives: 1) for general information, we screened 1377 PDCTs, with phase bioequivalency (BE) accounting for the majority (78.

Polymorphisms in MTNR1A (rs2119882) and CLOCK (rs1801260) genes are associated with facial acne susceptibility in gas station workers.

This study aimed to explore the relationship between circadian rhythm gene polymorphisms, specifically MTNR1A rs2119882 and CLOCK rs1801260, and the risk of acne in an occupational population. MTNR1A encodes a melatonin receptor involved in circadian rhythm regulation, while CLOCK is a core transcription factor in the molecular circadian clock. Both genes are essential in maintaining hormonal balance, sleep-wake cycles, and inflammatory responses-factors closely associated with acne pathogenesis.

Advances in Understanding Recurrent Pulmonary Infections Following Foreign Body Aspiration: A Narrative Review.

Recurrent pulmonary infections (RPIs) represent a common yet clinically complex entity, primarily triggered by aspiration or the presence of foreign bodies. They are notoriously insidious and challenging to detect clinically. These infections typically involve the invasion of bacteria, viruses, or fungi, leading to inflammation and damage of lung tissue.

[Glycyrrhetinic acid combined with doxorubicin induces apoptosis of human hepatocellular carcinoma HepG2 cells by regulating ERMMDs].

This study investigates the effect of glycyrrhetinic acid(GA) combined with doxorubicin(DOX) on apoptosis in HepG2 cells and its possible mechanisms. HepG2 cells were cultured in vitro, and cell viability was assessed using the cell counting kit-8(CCK-8) method. Flow cytometry was used to measure apoptosis levels in HepG2 cells.

Optimized genetic circuitry and reporters for sensitive whole-cell arsenic biosensors: advancing environmental monitoring.

The ubiquitous presence of arsenic pollution poses a significant threat to both ecosystem integrity and human health, necessitating the development of sensitive methods for arsenic detection. This study presents the development of innovative whole-cell biosensors that leverage the ArsR regulatory system within both naturally coupled and non-coupled genetic circuits, specifically optimized for detecting the highly toxic arsenic species (As(III)). These biosensors incorporate an indigoidine pigment as a reporting system and utilize the glycerol facilitator protein (GlpF) to enhance arsenic transport, offering a significant advantage over conventional detection methods by streamlining the detection process and eliminating the requirement for specialized instrumentation.

Pervasive but biome-dependent relationship between fragmentation and resilience in forests.

The relationship between landscape fragmentation and vegetation resilience is uncertain. Here we use multiple satellite-based tree cover data and vegetation indices to quantify the apparent effects of fragmentation on global forest resilience and potential mechanisms thereof. We measure fragmentation as edge density, patch density and mean patch area of tree cover patches, and measure resilience as one-lag temporal autocorrelation of vegetation indices.

Discovery of Novel Imidazothiazole-Based Hydroxamic Acid Derivatives as Potent Indoleamine 2,3-Dioxygenase 1 and Histone Deacetylase 6 Dual Inhibitors.

In order to take advantage of both immunotherapeutic and epigenetic antitumor agents, a series of imidazothiazole-based hydroxamic acid derivatives were designed based on the pharmacophore fusion strategy and evaluated as potent IDO1 and HDAC6 dual inhibitors. Among these inhibitors, the most potent compound () showed considerable IDO1 inhibitory activity and a good selectivity profile for HDAC6 over the other HDAC isoforms. The intracellular inhibition of HDAC6 by was validated by Western blot analysis.

Effects of Chinese herbal medicine on the secondary prevention of chemotherapy-induced thrombocytopenia in malignant solid tumors, a randomized clinical trial.

Objective: Thrombocytopenia is the main limiting toxicity of chemotherapy, which has not been adequately addressed until now. However, no exact studies have been conducted on the secondary prevention of chemotherapy-induced thrombocytopenia.The aim of this study is to explore the effect of Chinese herbal medicine on the secondary prevention of chemotherapy-induced thrombocytopenia (CIT) in malignant solid tumors.

The landscape of the microbiome at different stages of root caries.

Objective: To investigate the relationship between microorganisms and root caries, identify core species, and explore their interactions.

Materials And Methods: Thirty patients with different levels of root caries were included. Plaques from superficial (n = 30) and deep root caries (n = 30) and sound root surfaces (n = 30) were collected.

Magnetic Biochar Prepared with Residue as Adsorbents for Congo Red Removal.

In this work, magnetic biochars (MBCs) were produced with the chemical coprecipitation method. The resulting materials were dried at 50 °C for 12 h and characterized via SEM-EDS, XRD, FT-IR, BET, TGA, and VSM techniques to evaluate their efficacy in removing Congo red (CR). The effects of solution pH, CR concentration, MBC1:1 mass, and a variety of ions on the adsorption performance were systematically examined.

Fine-tuning of Wnt signaling by RNA surveillance factor Smg5 in the mouse craniofacial development.

The specific roles of nonsense-mediated mRNA decay (NMD), a translation-dependent RNA quality control mechanism that degrades mRNAs containing premature termination codons (PTCs), in mammalian craniofacial development have remained unclear. Here, we show that knockout of the essential NMD factor in mouse craniofacial neural crest cells leads to hypoplastic mandibles, subsequently inducing tongue mispositioning and cleft palate formation. Furthermore, loss triggers massive cell apoptosis and disrupts cell differentiation, accompanied by widespread alterations in alternative splicing and a surge in PTC-containing mRNA levels.

Development, Validation, and Visualization of a Web-Based Nomogram to Predict the Risk of Psychological Distress in Patients Undergoing Chemotherapy After Breast Cancer Surgery.

Background: Psychological distress of postoperative chemotherapy patients with breast cancer is significant and has a serious impact on their quality of survival. Risk prediction models can efficiently assess patients' psychological distress and risk factors.

Objectives: To investigate the factors influencing psychological distress in postoperative chemotherapy patients with breast cancer and construct a nomogram model to predict the occurrence of psychological distress in patients.

Optimal Method to Realize Quantitative Detection of 1D and 2D Nanoassemblies Based on AIE-Active Bolaamphiphilic Molecules.

Controllable transformation between the bolaamphiphilic molecule assemblies with different morphological nanostructures represents an exciting new direction for materials. However, there are still significant challenges for the quantitative detection and real-time monitoring of a controllable nanoself-assembly process due to insufficient measuring methods. Herein, we propose a new and effective fluorescence technology for realizing quantitative detection of a controllable conversion process of one-dimensional (1D)/two-dimensional (2D) nanoassemblies by introducing AIEgens as the fluorescence signal part.

Active Interfacial Perimeter in Pt/CeO Catalysts with Embedding Structure for Water-Tolerant Toluene Combustion.

Supported Pt catalysts are often subjected to severe deactivation under the conditions of high temperature and water vapor in catalytic oxidation; thus, the superior stability and water-resistant ability of catalysts have great significance for the effective degradation of volatile organic compounds (VOCs). Herein, we constructed a Pt/CeO-N catalyst with an active interfacial perimeter, in which Pt species were partially embedded in the defective CeO-N support to prevent the sintering. A significant charge transfer between Pt species and ceria in the embedding structure occurred via the Pt-CeO interface, which induced the formation of a Pt-O-Ce interfacial structure.

Comprehensive Analysis of Genes Across the Genome Uncovers Potential Functions in Sexual Reproduction and Abiotic Stress Tolerance.

The Domain of Unknown Function 506 () belongs to the PD-(D/E) XK nuclease superfamily and has been reported to play critical roles in growth and development as well as responses to abiotic stresses. However, the function of genes in () remains unclear. In this study, a total of 18 genes were identified and randomly distributed across eight chromosomes, categorized into four subfamilies.

A leaf age-dependent light use efficiency model for remote sensing the gross primary productivity seasonality over pantropical evergreen broadleaved forests.

Tropical and subtropical evergreen broadleaved forests (TEFs) contribute more than one-third of terrestrial gross primary productivity (GPP). However, the continental-scale leaf phenology-photosynthesis nexus over TEFs is still poorly understood to date. This knowledge gap hinders most light use efficiency (LUE) models from accurately simulating the GPP seasonality in TEFs.