Construction and characterization of Pickering emulsion stabilized by agar-phenylalanine complex microgel particles.

Polysaccharide-based food-grade microgel particles show promise as Pickering stabilizers when modified with biological components to optimize wettability. This study developed agar-amino acid complex microgel particles (AAMs) by integrating non-polar amino acids (e.g.

Fabrication and Characterization of Structure and Rheological Properties of Pea Protein-Gellan Gum Composite Gel.

Pea protein is rich in essential amino acids and has low allergenicity. In this study, pea protein was modified with gellan gum to enhance its gelation performance and improve its poor gelation properties. The effect of different concentrations of gellan gum on the properties of pea protein gel was investigated by analyzing hydration properties, texture, rheological characteristics, microstructure, and molecular forces.

Pre-prescription review system improves prescription safety, efficiency, and cost-effectiveness in pediatric outpatients: a western China study.

Objective: The study's objective is to investigate the prescription rationality rate, time efficiency, and estimated economic benefits of a pre-prescription review system (PPRS) in pediatric outpatients in a 5-year retrospective study in western China.

Method: This retrospective before-after study compared data from two phases: pre-intervention phase (2019: PPRS not yet implemented), and post-intervention phase (2020-2023: PPRS fully operational). The study evaluated three key endpoints: primary endpoint was the prescription rationality rate; the secondary endpoints were time efficiency (interval from prescription to drug dispensing) and suggested economic benefits (estimated benefit-cost ratio).

Efficient genome engineering in Agrobacterium tumefaciens C58 using recombineering assisted by CRISPR/Cas9.

Recombineering, a technique derived from phage-encoded homologous recombination, has emerged as a vital approach for bacterial genome engineering. Agrobacterium tumefaciens is extensively utilized to transfer DNA into the host plant genomes. To facilitate the transformation of various plant species, particularly those of considerable economic value, genetic modifications of Agrobacterium strains are essential.

Identification and verification of feature biomarkers associated with CD14 monocytes in type 1 diabetes.

Aims/introduction: Monocytes contribute to the immune dysregulation of pancreatic islets in Type 1 diabetes mellitus (T1D). This study aims to identify the feature genes of CD14 monocytes in T1D, which might offer a new perspective on immune dysregulation and potential therapeutic targets in T1D.

Materials And Methods: Two CD14 monocyte-related datasets were integrated for DEGs and WGCNA analysis.

Heterologous Expression of the Nitrogen-Fixing Gene Cluster from in .

Microbially mediated biological nitrogen fixation is pivotal to sustainable agricultural development. However, optimizing nitrogenase activity in native biological nitrogen-fixing bacteria has been hindered by the complexities of genetic manipulation. Heterologous expression has served as a foundational strategy for engineering next-generation nitrogen-fixing microbial agents.

A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces.

Background: Heterologous expression in Streptomyces provides a platform for mining natural products (NPs) encoded by cryptic biosynthetic gene clusters (BGCs) of bacteria. The BGCs are first engineered in hosts with robust recombineering systems, such as Escherichia coli, followed by expression in optimized heterologous hosts, such as Streptomyces, with defined metabolic backgrounds.

Results: We developed a highly efficient heterologous expression platform, named Micro-HEP (microbial heterologous expression platform), that uses versatile E.

Cattle welfare assessment based on adaptive fuzzy logic and multimodal data fusion.

This study proposes a cattle welfare evaluation method based on multi-modal data fusion, which integrates various data dimensions, such as cattle behavior characteristics, feeding management conditions, and environmental parameters, to achieve a systematic assessment of cattle welfare levels. The method establishes a quantitative scoring system based on behavioral duration and individual group differences, and designs a multi-modal data processing framework that combines Backpropagation (BP) neural networks with adaptive fuzzy logic. This framework uses a Gaussian membership function to replace the traditional triangular membership function for feature mapping, significantly improving the robustness and accuracy of the evaluation system through a differentiated weight allocation strategy.

Genomics-Driven Discovery of Plantariitin A, a New Lipopeptide in DSM9509.

A significant number of silent biosynthetic gene clusters (BGCs) within the genome remain uncharacterized, representing a valuable opportunity for the discovery of new natural products. In this research, the recombineering system ETh1h2e_yi23, which facilitates recombination in and was developed in our previous study, was used for mining the BGCs of DSM9509. By using this recombineering system, the constitutive promoter was precisely inserted into the genome, resulting in the activation of the silent BGC, which led to the production of a new lipopeptide named plantariitin A.

Redox-neutral decarboxylative coupling of fluoroalkyl carboxylic acids dual metal photoelectrocatalysis.

Given the importance and beneficial characteristics of aliphatic CF chiral compounds in modern chemistry, efficient strategies for their synthesis are highly sought after. While α-CF carboxylic acid is an emerging and easily accessible CF-containing synthon, its use as a source of fluoroalkyl is highly challenging due to its high oxidation potential. Herein, we disclose a photoelectrocatalytic method for the direct and enantioselective decarboxylative cross-coupling of α-CF carboxylic acids.

Development Trends and Prospects of Technology-Based Solutions for Health Challenges in Aging Over the Past 25 Years: Bibliometric Analysis.

Background: As the global population ages, we witness a broad scientific and technological revolution tailored to meet the health challenges of older adults. Over the past 25 years, technological innovations, ranging from advanced medical devices to user-friendly mobile apps, are transforming the way we address these challenges, offering new avenues to enhance the quality of life and well-being of the aging demographic.

Objective: This study aimed to systematically review the development trends in technology for managing and caring for the health of older adults over the past 25 years and to project future development prospects.

Association between Vitamin E, Vitamin B6, and Vitamin B12 with coronary heart disease.

Conflicting evidence still exists regarding Vitamin B12's involvement in coronary heart disease (CHD). There is no precedent for previous studies to include both Vitamin B12, Vitamin B6, as well as Vitamin E in the consideration of CHD associating factors. Our data derived from the National Health and Nutrition Examination Survey (NHANES), which covers the period 2003-2020.

Bis(phosphinophenyl)amido-Ligated Binuclear Rare-Earth Metal Complexes for Highly cis-1,4-Selective Polymerization of 1,3-Conjugated Dienes.

A series of binuclear rare-earth metal complexes based on the ligands containing bis(phosphinophenyl)amido-PNP unit are successfully synthesized. All the ligands and the corresponding binuclear complexes are fully characterized by NMR spectra (H, C, and P). In conjunction with [PhC][B(CF)], all the binuclear complexes exhibited high catalytic activity and high cis-1,4-selectivity (>99%) toward the polymerization of 1,3-conjugated dienes (isoprene, β-myrcene and β-farnesene) with excellent livingness at room temperature or even 80 °C.

Learning implementation of a guideline based decision support system to improve hypertension treatment in primary care in China: pragmatic cluster randomised controlled trial.

Objective: To evaluate the effectiveness of a clinical decision support system (CDSS) in improving the use of guideline accordant antihypertensive treatment in primary care settings in China.

Design: Pragmatic, open label, cluster randomised trial.

Setting: 94 primary care practices in four urban regions of China between August 2019 and July 2022: Luoyang (central China), Jining (east China), and Shenzhen (south China, including two regions).

Binuclear Enamino-Oxazolinate Rare-Earth Metal Complexes: Synthesis and Their Catalytic Performance in Isoprene Polymerization.

We have synthesized a series of binuclear rare-earth metal complexes bearing the newly designed enamino-oxazolinate ligands that feature bridging -phenyl, -phenyl, 1,5-naphthalenyl, and 1,5-anthracenyl moieties. NMR and X-ray diffraction analyses confirmed the binuclear structures of the obtained complexes with two enamino-oxazolinate-metal units located at a position against the bridged aryl plane. After activation by [PhC][B(CF)], all the rare-earth metal complexes served as efficient catalysts for isoprene polymerization, producing polymers with high 1,4 regularity (up to 96.

The U-shaped association between remnant cholesterol and risk of all-cause and cardiovascular deaths in diabetic adults: Findings from NHANES 1999-2018.

Background And Aims: We aimed to explore the association between remnant cholesterol (RC) level and risks of all-cause and cardiovascular deaths among American diabetic adults.

Methods And Results: The data of 4,095 diabetic participants from the National Health and Nutrition Examination Survey (1999-2018) were included for analysis. Deaths were ascertained till December 31, 2019.

Characteristics analysis of Internet pharmacy consultation services for children in southwest China during the post-epidemic era: A cross-sectional study.

Aim: This study aims to examine the features of online pediatric pharmacy consultations in Southwest China, an area with limited medical resources during the post-epidemic era and to explore the factors affecting the dialogue volume in online consultations.

Methods: A cross-sectional study was conducted in southwest China from April 2022 to March 2023. The study encompassed a cohort of 2,526 children, ranging from 0 to 18 years old.

Ultrapure single-band red upconversion luminescence in Er doped sensitizer-rich ytterbium oxide transparent ceramics for solid-state lighting and temperature sensing.

Achieving single-band upconversion (UC) is a challenging but rewarding approach to attain optimal performance in diverse applications. In this paper, we successfully achieved single-band red UC luminescence in YbO: Er transparent ceramics (TCs) through the utilization of a sensitizer-rich design. The YbO host, which has a maximum host lattice occupancy by Yb sensitizers, facilitates the utilization of excitation light and enhances energy transfer to activators, resulting in improved UC luminescence.

A recombineering system for based on the native phage recombinase pair YqaJ/YqaK.

plays an important role in fundamental and applied research, and it has been widely used as a cell factory for the production of enzymes, antimicrobial materials, and chemicals for agriculture, medicine, and industry. However, genetic manipulation tools for have low efficiency. In this work, our goal was to develop a simple recombineering system for .

QX-type infectious bronchitis virus infection in roosters can seriously injure the reproductive system and cause sex hormone secretion disorder.

Since its discovery, QX-type avian infectious bronchitis virus (IBV) has rapidly spread worldwide and become the most prevalent dominant genotype in Asia and Europe. Currently, although the pathogenicity of QX-type IBV in the reproductive system of hens is widely and deeply understood, its pathogenicity in the reproductive system of roosters remains largely unknown. In this study, 30-week-old specific pathogen-free (SPF) roosters were used to investigate the pathogenicity of QX-type IBV in the reproductive system after infection.