3D-printed vancomycin-eluting PGCL/MXene bifunctional scaffold for management of infected bone defects.

The clinical management of infected bone defects remains a significant challenge in orthopedic surgery. In this study, we developed a multifunctional composite scaffold by integrating MXene (TiC) nanosheets and vancomycin (Van) into a degradable poly(glycolide-co-caprolactone) (PGCL) polymer through precision 3D printing technology, with the aim of regenerating acutely infected bone defects. Systematic optimization established the optimal composition as 5 wt% TiC and 5 wt% Van within the degradable PGCL polymer.

SKI-606, a Src inhibitor, ameliorates benzene-induced hematotoxicity via blocking ROS/Src kinase-mediated p38 and Akt signaling pathways.

Exposure to benzene causes acute myelosuppression and other hematologic disorders. However, the detailed mechanism by which benzene exerts its severe hematotoxicity and potential treatments still require further deciphering and exploration. Herein, we found that hydroquinone (HQ), a main benzene metabolite, significantly increased intracellular reactive oxygen species (ROS) formation and subsequently caused damage to DNA, leading to impaired colony formation capacity and induction of apoptosis in human hematopoietic stem/progenitor cells (HSPCs) in vitro.

Parametric Linear Blend Skinning Model for Multiple-Shape 3D Garments.

We present a novel data-driven Parametric Linear Blend Skinning (PLBS) model meticulously crafted for generalized 3D garment dressing and animation. Previous data-driven methods are impeded by certain challenges including overreliance on human body modeling and limited adaptability across different garment shapes. Our method resolves these challenges via two goals: 1) Develop a model based on garment modeling rather than human body modeling.

Correction: Polydiacetylene-based colorimetric and fluorometric sensors for lead ion recognition.

[This corrects the article DOI: 10.1039/D2RA03435B.].

Incidence and risk factors of subsequent vertebral fracture following percutaneous vertebral augmentation in postmenopausal women.

Purpose: Subsequent vertebral fracture (SVF) is a severe advent event of percutaneous vertebral augmentation (PVA). However, the incidence and risk factors of SVF following PVA for OVCF in postmenopausal women remain unclear. This research aims to investigative the incidence and risk factors of SVF after PVA for OVCF in postmenopausal women.

Directed Evolution of Escherichia coli Nissle 1917 to Utilize Allulose as Sole Carbon Source.

Sugar substitutes are popular due to their akin taste and low calories. However, excessive use of aspartame and erythritol can have varying effects. While D-allulose is presently deemed a secure alternative to sugar, its excessive consumption is not devoid of cellular stress implications.

Research on brain functions related to visual information processing and body coordination function of pilots based on the low-frequency amplitude method.

Objective: Research on the differences in physiological and psychological mechanisms of practitioners in different occupations is a current hot spot, such as pilots. This study explores the frequency-dependent changes of pilots' low-frequency amplitudes in the classical frequency band and sub-frequency band between pilots and general occupations. The goal of the current work is to provide objective brain images for the selection and evaluation of outstanding pilots.

Analysis of CT imaging changes of psoas major muscles in patients with lumbar disc herniation mainly based on low back pain and lower limb pain.

Background: The study aimed to compare the area changes of CT (computed tomograghy) imaging of psoas major muscle (PM) in patients with lumbar disc herniation (LDH) mainly based on low back pain (LBP) and lower limb pain (LLP), and to analyze the correlation among them.

Methods: We retrospectively analyzed the lumbar CT imaging data of 120 patients with LDH and 60 healthy control people in our hospital from July 2017 to August 2019. They were divided into LBP group (60 cases), LLP group (60 cases) and healthy controls group (60 cases).

Intradural lumbar disc herniation of L2-L3: A case report and literature review.

Background: Intradural lumbar disc herniation (ILDH), especially upper lumbar intradural disc herniation, is a rare type of lumbar disc herniation (LDH). However, it may have severe and complex symptoms, causing serious impact on the patients. Additionally, it is difficult to be diagnosed with limited experience.

Percutaneous endoscopic interlaminar discectomy for L5-S1 calcified lumbar disc herniation: A retrospective study.

Background: Calcified lumbar disc herniation (CLDH) is considered to be a special type of lumbar disc herniation (LDH). Percutaneous endoscopic interlaminar discectomy (PEID), with safety and efficacy, has been proved to be a minimally invasive surgery for LDH. However, there are few studies on PEID in the treatment of CLDH at the L5-S1 level.

Polydiacetylene-based colorimetric and fluorometric sensors for lead ion recognition.

Development of novel sensors for the detection of lead ions (Pb) has attracted increasing interest due to their inherent toxic effects on human health and the environment. In this study, we describe two new polydiacetylene (PDA)-based liposome sensors for the colorimetric and fluorometric recognition of Pb in aqueous solution. In the sensor system, a thymine-1-acetic acid (TAA) or orotic acid (OA) group was reasonably introduced into the diacetylene monomer to work as a strong binding site for Pb.

A versatile Cas12k-based genetic engineering toolkit (C12KGET) for metabolic engineering in genetic manipulation-deprived strains.

The genetic modification of microorganisms is conducive to the selection of high-yield producers of high-value-added chemicals, but a lack of genetic tools hinders the industrialization of most wild species. Therefore, it is crucial to develop host-independent gene editing tools that can be used for genetic manipulation-deprived strains. The Tn7-like transposon from Scytonema hofmanni has been shown to mediate homologous recombination-independent genomic integration after heterologous expression in Escherichia coli, but the integration efficiency of heterologous sequences larger than 5 kb remains suboptimal.

The Neural Underpinnings of Emotional Conflict Control in Pilots.

Piloting an aircraft is a complex cognitive task. Human error represents a major contributing factor in aviation accidents. Emotion plays an important role in aviation safety.

Increased functional dynamics in civil aviation pilots: Evidence from a neuroimaging study.

Civil aviation is a distinctive career. Pilots need to monitor the entire system in real time. However, the psychophysiological mechanism of flying is largely unknown.

Altered Default Mode Network Dynamics in Civil Aviation Pilots.

Background: Airlines occupy an increasingly important place in the economy of many countries. Because air disasters may cause substantial losses, comprehensive surveys of the psychophysiological mechanism of flying are needed; however, relatively few studies have focused on pilots. The default mode network (DMN) is an important intrinsic connectivity network involved in a range of functions related to flying.

Cascade reaction-based trinal-site probe for sensing and imaging of cysteine and glutathione.

Biothiols, such as glutathione (GSH) and cysteine (Cys), play important roles in many physiological processes, and variations in their levels are related to different diseases. Many fluorescence probes have been developed to understand the function of biothiols, but only few of such probes can detect both Cys and GSH. Herein, a new strategy for specific colorimetric and fluorescent detection of Cys and GSH by different cascade reactions was developed.

MiR-27a targets DKK2 and SFRP1 to promote reosseointegration in the regenerative treatment of peri-implantitis.

In the inflamed microenvironment of peri-implantitis, limited osteogenesis on the implant surface impedes well-established reosseointegration using current clinical therapies. MicroRNAs (miRNAs) function as potent molecular managers that may simultaneously regulate multiple endogenous processes such as inflammation and osteogenesis. The delivery of miRNAs may provide a way to effectively treat some diseases.

Regioselectivity inversion tuned by iron(iii) salts in palladium-catalyzed carbonylations.

Impactful regioselectivity control is crucial for cost-effective chemical synthesis. By using cheap and abundant iron(iii) salts, the hydroxycarbonylations of both aromatic and aliphatic alkenes were significantly enhanced in both reactivity and selectivity (iso/n or n/iso up to >99 : 1). Moreover, Pd-catalyzed carbonylation selectivity can be switched from branched to linear by using different Fe(iii) salts.

Elevated FKBP52 expression indicates a poor outcome in patients with breast cancer.

The 52-kDa FK506-binding protein (FKBP52), a regulator of steroid hormone receptor signaling, is potentially involved in a variety of hormone-dependent cancer types. The present study investigated the expression and clinical implications of FKBP52 in breast cancer. Immunohistochemistry was performed on samples from 145 breast cancer patients and on 66 unmatched breast non-cancerous tissues (as controls) to determine the expression level of FKBP52.

MicroRNA sequence analysis identifies microRNAs associated with peri-implantitis in dogs.

Peri-implantitis, which is characterized by dense inflammatory infiltrates and increased osteoclast activity, can lead to alveolar bone destruction and implantation failure. miRNAs participate in the regulation of various inflammatory diseases, such as periodontitis and osteoporosis. Therefore, the present study aimed to investigate the differential expression of miRNAs in canine peri-implantitis and to explore the functions of their target genes.

miR-33a-5p modulates TNF-α-inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs.

miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p.