Synthesis of nanoscale zero-valent iron by one-pot route and study of its potential in passivating coexistent heavy metal anions and cations in soil.

Nanoscale zero-valent iron (nZVI) was synthesized by a one-pot liquid-phase chemical method in the presence of FeSO as the iron source and NaBH as the reducing agent. The synthesized nZVI was characterized by scanning electron microscopy, X-ray diffraction, energy dispersive spectrometry, and Fourier transform infrared spectroscopy. Its ability to passivate Pb, Cd, and AsO in soils was evaluated by inductively coupled plasma-atomic emission spectroscopy, and the passivation mechanism was explored based on adsorption thermodynamics and kinetics simulations.

Identify doctors' online performance and prioritize service attributes: A framework with aspect-based sentiment analysis and empirical investigation.

Objective: This study aims to prioritize service attributes related to doctors' online performance based on patients' reviews on online healthcare platforms (OHPs).

Method: We propose a three-stage framework based on uncertainty reduction theory. First, perceived service attributes are extracted from review texts through aspect-based sentiment analysis using deep learning models.

Dwell time and group delays in complex media.

We analyze the dwell time and group delays of electromagnetic waves during transmission and tunneling through complex media, which are non-Hermitian and dispersive. We show that the dwell time, group delays of reflection and transmission, and Dicke times are no longer equal, even though the structure remains mirror symmetric. An additional contribution from the dissipative response of the complex media - termed the energy loss time - must be taken into account.

Metabolic Engineering of Methanotrophic Bacteria for Production of Taxadiene from Methane.

The growing demand for natural products in pharmaceutical applications has prompted a focus on more sustainable methods to produce high-value terpenoids using microbial cell factories. Due to the characteristics of high abundance, renewable, and low cost, methane has emerged as a promising feedstock for biomanufacturing. In this study, the methanotrophic bacterium 5GB1C, known for its industrial potential, was metabolically engineered to synthesize taxadiene, a crucial precursor in paclitaxel production.

Coupling Photocatalytic Reduction and Biosynthesis Towards Sustainable CO Upcycling.

Upcycling carbon dioxide (CO) into long-chain compounds has attracted considerable attention with respect to mitigating environmental problems and obtaining value-added feedstocks, but remains a great challenge. Herein, we report a tandem photocatalysis-biosynthesis strategy for efficient CO reduction to energy-rich sucrose or α-farnesene. Firstly, photocatalytic reduction of CO to CH was optimized over the transitional metal doped ZnO (M-ZnO).

Gain-of-function ANXA11 mutation cause late-onset ALS with aberrant protein aggregation, neuroinflammation and autophagy impairment.

Mutations in the ANXA11 gene, encoding an RNA-binding protein, have been implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS), but the underlying in vivo mechanisms remain unclear. This study examines the clinical features of ALS patients harboring the ANXA11 hotspot mutation p.P36R, characterized by late-onset motor neuron disease and occasional multi-system involvement.

Genotype-phenotype association and functional analysis of mutations in amyotrophic lateral sclerosis.

Background: Pathogenic variants in have been reported in amyotrophic lateral sclerosis (ALS) patients. However, studies on mutant spectrum and pathogenicity of variants were rare.

Methods: We performed whole exome sequencing of ALS-associated genes and subsequent verification of rare variants in in our ALS patients.

(Eu-MOF)-derived Smart luminescent sensing for Ultrasensitive on-site detection of MiR-892b.

MicroRNAs (miRNAs) are important biomacromolecules used as biomarkers for the diagnosis of several diseases. However, current detection strategies are limited by expensive equipment and complicated procedures. Here, we develop a portable, sensitive, and stable (Eu-MOF)-based sensing platform to detect miRNA via smartphone.

Non-Hermitian guided modes and exceptional points using loss-free negative-index materials.

We analyze the guided modes in coupled waveguides made of negative-index materials without gain or loss. We show that it supports non-Hermitian phenomenon on the existence of guided mode versus geometric parameters of the structure. The non-Hermitian effect is different from parity-time ( ) symmetry, and can be explained by a simple coupled-mode theory with an anti- symmetry.

Revisit the Poynting vector in -symmetric coupled waveguides.

We show that the time-averaged Poynting vector of →=→×→/2 in parity-time ( ) symmetric coupled waveguides is always positive and cannot explain the stopped light at exceptional points (EPs). In order to solve this paradox, we must accept the fact that the fields → and → and the Poynting vector in non-Hermitian systems are in general complex. Based on the original definition of the instantaneous Poynting vector →=→×→, a formula on the group velocity is proposed, which agrees perfectly well with that calculated directly from the dispersion curves.

Cloning of Nitrate Reductase and Nitrite Reductase Genes and Their Functional Analysis in Regulating Cr(VI) Reduction in Ectomycorrhizal Fungus sp.1.

Assimilatory-type nitrate reductase (NR) and nitrite reductase (NiR) are the key enzymes that involve in nitrate assimilation and nitrogen cycling in microorganisms. NR and NiR with NADH or NADPH and FMN or FAD domains could be coupled to the reduction process of hexavalent chromium [Cr(VI)] in microorganisms. A new assimilatory-type NR gene (named ) and a new assimilatory-type NiR gene (named ) are cloned, identified, and functionally characterized by 5' and 3' RACE, alignment, annotation, phylogenetic tree, and yeast mutant complementation analyses from sp.

Diversity and community structure of ectomycorrhizal fungi in Pinus thunbergii coastal forests bordering the Yellow Sea of China.

Ectomycorrhizas play a fundamental role in the function of forest ecosystems, being essential for plant nutrition absorption and soil quality. Many afforestation and reforestation programmes have begun to recover and maintain coastal forests in China, using pine species including Pinus thunbergii. We investigated the ectomycorrhizal colonization status of P.

Coupling analysis of contra-rotating fan interstage pressure pulsation and blade vibration based on wavelet reconstruction.

In recent years, the flow characteristics research of the interstage region in counter-rotating axial fans in terms of fluid dynamics has attracted much attention. Especially, the dynamic relationship between interstage pressure pulsation and blade vibration in counter-rotating axial fans has not yet been clarified. This paper performs the signal processing method of wavelet decomposition and reconstruction in time-frequency analysis process.

Dynamical analysis on a predator-prey model with stage structure and mutual interference.

In this paper, we formulate a stage-structured predator-prey model with mutual interference, in which includes two discrete delays. By theoretical analysis, we establish the stability of the unique positive equilibrium and the existence of Hopf bifurcation when the maturation delay for predators is used as the bifurcation parameter. Our results exhibit that the maturation delay for preys does not affect the stability of the positive equilibrium.

The great potential for phytoremediation of abandoned tailings pond using ectomycorrhizal Pinus sylvestris.

To explore the potential of ectomycorrhizal (ECM) Pinus sylvestris (P. sylvestris) utilizing in the phytoremediation of a combined heavy metal contaminated tailings pond, Pisolithus sp.1(P1)-.

Earthworm activities weaken the immobilizing effect of biochar as amendment for metal polluted soils.

The effect of earthworms on the immobilization of heavy metals by biochar was investigated using 4-week soil pot experiments. Our results showed that both sludge and rice husk biochars applied to Cd, Pb and Zn contaminated soils significantly reduced the bioavailability of those heavy metals in soils, and their concentrations in soil pore water. The Cd and Pb concentrations in pakchoi shoots were decreased by 10.

Nuclear magnetic resonance structure of the P395S mutant of the N-SH2 domain of the p85 subunit of PI3 kinase: an SH2 domain with altered specificity.

Understanding the specificity of Src homology 2 (SH2) domains is important because of their critical role in cell signaling. Previous genetic analysis has characterized mutants of the N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K). The P395S mutant exhibits a specificity for phosphopeptide binding different from that of the wild-type SH2.