Application of a Rationally Designed Mucosal Nanovaccine Platform in Bacterial Disease Prophylactics Using Fish as a Model Organism.

The emergence of antimicrobial resistance necessitates innovative vaccine strategies for bacterial disease prevention in aquaculture. Here, we present a rationally designed mucosal nanovaccine platform targeting , a major pathogen in marine fish. Bioinformatics tools identified 15 T-cell and 16 B-cell epitopes from 17 antigenic proteins, which were consolidated into a multiepitope antigen (MAE).

Gastric tube-guided and robot-assisted laparoscopic resection of gastroesophageal junction stromal tumors: Two case reports.

Background: Gastroesophageal junction (GEJ) or gastrointestinal stromal tumor (GIST) are located in unfavorable parts of the stomach, due to the anatomical complexity of these regions, protecting the cardia while ensuring R0 resection is a major challenge for surgeons.

Case Summary: Two cases of GEJ stromal tumors were reported. Abdominal computed tomography scans revealed that both tumors were located at the GEJ, close to the posterior wall, with one tumor measuring greater than 5 cm.

Rationally designed self-assembled peptide nanofibers provoke robust humoral immunity against nervous necrosis virus.

Nervous necrosis virus (NNV) poses devastating mortality risks to almost all marine fish, causing significant economic losses to the marine fish aquaculture industry, particularly in juvenile groupers. However, no effective NNV vaccine is available. To counter this, we report a smart strategy of a rationally designed modular peptide vaccine platform (CP-Q11) that recapitulates the antigen peptide CP and a Q11 self-assembling domain.

Photodimerization of norbornenes and cyclohexenes catalyzed by Cu(I) complexes: mechanistic similarities and differences.

The Cu(I)-photocatalyzed cycloaddition reaction has become an important way to construct four-membered ring products. A recent experiment reported the photocycloaddition of norbornenes (nb) and cyclohexenes (ch) catalyzed by CuOTf. However, the atomic level details remain lacking.

Differential Infection Dynamics of in Dicotyledonous (Cotton) versus Monocotyledonous (Maize) Host Systems.

Verticillium dahliae, a soil-borne fungal pathogen, infects and causes wilt symptoms in dicot but not in monocot plants. The precise sequence of events when V. dahliae infects monocots remain unclear.

Developing and validating a machine learning-based model for predicting in-hospital mortality among ICU-admitted heart failure patients: A study utilizing the MIMIC-III database.

Background: Although the assessment of in-hospital mortality risk among heart failure patients in the intensive care unit (ICU) is crucial for clinical decision-making, there is currently a lack of comprehensive models accurately predicting their prognosis. Machine learning techniques offer a powerful means to identify potential risk factors and predict outcomes within multivariable clinical data.

Methods: This study, based on the MIMIC-III database, extracted demographic characteristics, vital signs, laboratory test values, and comorbidity information of heart failure patients using structured query language.

Prediction of perioperative complications in colorectal cancer artificial intelligence analysis of heart rate variability.

Background: Heart rate variability (HRV) represents efferent vagus nerve activity, which is suggested to be related to fundamental mechanisms of tumorigenesis and to be a predictor of prognosis in various cancers. Therefore, this study hypothesized that HRV monitoring could predict perioperative complication (PC) in colorectal cancer (CRC) patients.

Aim: To investigate the prognostic value of HRV in hospitalized CRC patients.

Asprosin-FABP5 Interaction Modulates Mitochondrial Fatty Acid Oxidation through PPARα Contributing to MASLD Development.

Alterations in liver metabolism play a pivotal role in the development and progression of metabolic dysfunction-associated steatotic liver disease (MASLD). Asprosin is reported to be released from white adipose tissue during fasting and targets the liver. However, the role of asprosin, especially from organs other than adipose tissue, in MASLD remains poorly understood.

Development and evaluation of recombinant multi-epitopes vaccine against nervous necrosis virus.

Capsid protein (CP) is the antigen of nervous necrosis virus (NNV), a fatal microorganism for almost marine fishes. Antigen epitope is a special chemical group in an antigen molecule that determines the specificity of the antigen, usually consisting of 5-15 amino acid residues. However, the antigen epitope of NNV antigen remains unclear.

Research Progress on Biosynthesis and Regulation of Conjugated Linoleic Acid in Lactic Acid Bacteria.

Conjugated linoleic acid (CLA) has gained considerable attention and has been extensively studied owing to its physiological functions, including weight loss, immune regulation, and anti-carcinogenesis. However, the CLA content is very low in natural sources and insufficient to exert a probiotic effect. Additionally, its chemical synthesis is limited by the lack of isomer selectivity, harsh reaction conditions, and the introduction of organic solvents.

Synthesis of NIR-II Fluorophores by a C(sp)-C(sp/sp) Coupling Reaction Driven by Charge-Transfer Interaction.

Here, we report a C(sp)-C(sp/sp) coupling reaction driven by charge transfer interaction. Due to the strong intermolecular noncovalent interaction, an essential electron donor-acceptor (EDA) complex is formed, confirmed solidly by absorption spectra, H NMR and single crystal X-ray diffraction. The EDA complex lowers the activation barrier (E=9.

Hydrophobic vehicles for hydrophilic drugs: Sustained intravitreal caffeine delivery with oleogels.

Caffeine is the most widely consumed bioactive ingredient in the world, which has been found to show great therapeutic potential in several posterior eye diseases. While intravitreal injection represents the ideal administration route for these disorders, it remains challenging to achieve sustained release of caffeine in the vitreous. Herein, we address this issue by loading crystalline caffeine within oleogels (Ca@oleogels), oily delivery vehicles which provide a hydrophobic environment that is opposite to the hydrophilic nature of their cargos.

Na-K-ATPase/GLT-1 interaction participates in EGCG protection against cerebral ischemia-reperfusion injury in rats.

Background: In China, stroke is the primary cause of adult death and disability. Because of the increased rate of blood vessel reperfusion, it is important to prevent cerebral ischemia-reperfusion injury, in which glutamate (Glu) excitotoxicity plays a critical role. The most important Glu transporter, GLT-1, is essential for the regulation of Glu, which is dependent on Na-K-ATPase (NKA)-induced ion concentration gradient differences.

Unveiling the therapeutic role of Dachaihu decoction in acute cholecystitis: a comprehensive systematic review and meta-analysis of its efficacy and safety.

Background: Dachaihu decoction (Dachaihu tang) plays a crucial role in treating acute illnesses. Recently, a significant number of clinical studies on Dachaihu decoction for acute cholecystitis (AC) have been published. This study was conducted to assess the efficacy and safety of Dachaihu decoction in patients with this condition.

Regulatory Effect of Transcriptional Regulator TetR on the Synthesis of Conjugated Linoleic Acid in AR195.

Conjugated linoleic acid (CLA) possesses anticancer, anti-inflammatory, and antiobesity properties, making it a significant research focus. In this study, we identified TetR, a TetR/AcrR family transcriptional regulator encoded by , as the transcriptional regulator of CLA synthesis in AR195. TetR binds to the promoter regions of the CLA synthesis genes, including the operon and , thereby enhancing CLA biosynthesis.

In Situ Gelling Eye Drops of Tacrolimus with Improved Ocular Delivery and Therapeutic Efficacy.

In situ gelling eye drops of tacrolimus (FK506 Gel) were developed to address the formulation challenge of tacrolimus for anterior ocular inflammatory diseases. Both in silico and in vitro investigations were conducted to screen a suitable cyclodextrin species to increase the drug solubility. Guanosine was employed as the gelator and combined with inclusion complexes of tacrolimus in the presence of borate anions to obtain FK506 Gel, which gelated when came into contact with cations in tear fluid and led to the formation of a nanofibrous hydrogel.

Synergistic impact of plasma albumin and cognitive function on all-cause mortality in Chinese older adults: a prospective cohort study.

Background: Hypoalbuminemia and cognitive impairment (CI) each independently increase the mortality risk in older adults. However, these two geriatric syndromes can occur simultaneously. In community-dwelling older adults, is the combination of hypoalbuminemia and CI linked to a higher mortality risk than either condition alone?

Objective: We aimed to investigate the association between plasma albumin, cognitive function, and their synergistic effect on mortality in Chinese community-dwelling older adults.

Sharp needle reconstructs peripheral outflow for patients with malfunctional arteriovenous fistula.

Objective: To investigate the feasibility and efficacy of combining ultrasound-guided sharp needle technique with percutaneous transluminal angioplasty (PTA) for treating outflow stenosis or dysfunction in arteriovenous fistula (AVF) among hemodialysis patients.

Methods: From October 2021 to March 2023, patients with occluded or malfunctional fistula veins not amenable to regularly angioplasty were retrospectively enrolled in the study. They underwent ultrasound-guided sharp needle intervention followed by PTA.

Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.

2,5-Dimethylpyrazine (2,5-DMP) is important pharmaceutical raw material and food flavoring agent. Recently, engineering microbes to produce 2,5-DMP has become an attractive alternative to chemical synthesis approach. In this study, metabolic engineering strategies were used to optimize the modified Escherichia coli BL21 (DE3) strain for efficient synthesis of 2,5-DMP using L-threonine dehydrogenase (EcTDH) from Escherichia coli BL21, NADH oxidase (EhNOX) from Enterococcus hirae, aminoacetone oxidase (ScAAO) from Streptococcus cristatus and L-threonine transporter protein (EcSstT) from Escherichia coli BL21, respectively.

Application of electroacupuncture in the prevention of low anterior resection syndrome after rectal cancer surgery.

Background: Low anterior resection syndrome (LARS) is one of the common postoperative complications in patients with rectal cancer, which seriously affects their postoperative recovery and quality of life (QoL). Electroacupuncture therapy is one of the characteristic therapies of traditional Chinese medicine. There are few reports on the prevention and treatment of LARS by electroacupuncture therapy.

The effect of China's birth policy changes on birth defects-A large hospital-based cross-sectional study.

A retrospective analysis of birth data hospital-based obtained from 14 monitoring areas in the Huaihe River Basin from 2009 to 2019 was conducted. Trend in the total prevalence of birth defects (BDs) and subgroups were analyzed using the Joinpoint Regression model. The incidence of BDs increased gradually from 118.

Transcutaneous Electrical Acupoint Stimulation Improves Postoperative Sleep Quality in Patients Undergoing Laparoscopic Gastrointestinal Tumor Surgery: A Prospective, Randomized Controlled Trial.

Introduction: This study was conducted to observe the effect of transcutaneous electrical acupoint stimulation (TEAS) on the postoperative sleep quality of patients undergoing gastrointestinal tumor surgery and to verify the possible mechanism.

Methods: Eighty-three patients were allocated to the TEAS or Sham group. Patients in the TEAS group received TEAS treatment (disperse-dense waves; frequency, 2/100 Hz) on bilateral Shenmen (HT7), Neiguan (PC6) and Zusanli (ST36) points for 30 min each time, total three times in the perioperative period.