Tooth Root Development and Homeostasis during Eruptive and Post-eruptive Movement.

Tooth eruption is the process whereby the developing tooth moves to its functional position in the occlusal plane and it occurs concomitantly with formation of the tooth root, which is a critical component of the tooth anchored to surrounding alveolar bone through the periodontal ligament. Post-eruptive tooth movement ensues that once occlusion is achieved, the teeth maintain their alignment within the alveolar bone to facilitate proper bite function through periodontium remodelling. Tooth overeruption presents a clinically significant issue, yet the precise mechanisms by which alterations in occlusal forces are translated into periodontal remodelling remain largely unexplored.

Potential therapeutic role of spermine via Rac1 in osteoporosis: Insights from zebrafish and mice.

Osteoporosis is a prevalent metabolic bone disease. While drug therapy is essential to prevent bone loss in osteoporotic patients, current treatments are limited by side effects and high costs, necessitating the development of more effective and safer targeted therapies. Utilizing a zebrafish ( ) larval model of osteoporosis, we explored the influence of the metabolite spermine on bone homeostasis.

Nicotinamide Mononucleotide Alleviates Osteoblast Senescence Induction and Promotes Bone Healing in Osteoporotic Mice.

Combating the accumulated senescent cells and the healing of osteoporotic bone fractures in the older remains a significant challenge. Nicotinamide mononucleotide (NMN), a precursor of NAD+, is an excellent candidate for mitigating aging-related disorders. However, it is unknown if NMN can alleviate senescent cell induction and enhance osteoporotic bone fracture healing.

Osteogenic Induction of Low-dose Ipriflavone on Bone Marrow Mesenchymal Stem Cells Extracted from Osteoporosis Rats.

Objective: To investigate the effects of ipriflavone (IPF), a synthetic isoflavone plant oestrogen with a structure similar to that of oestradiol, on the osteogenic differentiation of bone mesenchymal stem cells (BMSCs).

Methods: BMSCs were derived from ovariectomised rats (rBMSCs-OVX) and then induced with or without IPF. Cell cytotoxicity, mineralisation in vitro and osteoblast-specific gene expression of BMSCs were studied.

The Crucial Role of Wntless in Osteogenesis and Odontogenesis.

Wnt signalling pathways have been the focus of intense research activity for decades due to their fundamental role in skeletal and dental development. Wntless, an exclusive chaperone protein for the exocytotis of Wnt ligands, was identified in 2006. In the last decade, the molecular biological studies of Wntless and its genetic studies in human and mice have highlighted the importance of this protein in mineralised tissues, including bone, cartilage and teeth.

Validation of Digital Evaluation in Systematic Training on Tooth Preparation in Aesthetic Veneer Rehabilitation.

Objective: To evaluate the outcome of systematic video training on tooth preparation in veneer restoration and the practicability of the application of the digital evaluation system of scan design and assessment software.

Methods: Ten residents were selected from a group enrolled on the first-year programme for the National Standard Training of Dentistry in the Department of Prosthodontics, College of Stomatology, Ninth People's Hospital Affliated with Shanghai Jiao Tong University, School of Medicine. First, each student prepared five teeth based on their knowledge and clinical experience, and then received systematic video training on veneer preparation.

[Advances in biomimetic modification of materials for oromaxillofacial bone regeneration and dental implant].

Oromaxillofacial hard tissue defects is still a difficult problem in clinical treatment. Regeneration of oromaxillofacial hard tissue based on tissue engineering technology has a good clinical application prospect. The functional modification of scaffolds is one of key factors that influence the outcome of tissue regeneration.

Baghdadite Ceramics Prevent Senescence in Human Osteoblasts and Promote Bone Regeneration in Aged Rats.

Bone fractures and critical-sized bone defects present significant health threats for the elderly who have limited capacity for regeneration due to the presence of functionally compromised senescent cells. A wide range of synthetic materials has been developed to promote the regeneration of critical-sized bone defects, but it is largely unknown if a synthetic biomaterial (scaffold) can modulate cellular senescence and improve bone regeneration in aged scenarios. The current study investigates the interaction of Baghdadite (CaZrSiO) ceramic scaffolds with senescent human primary osteoblast-like cells (HOBs) and its bone regeneration capacity in aged rats.

Associations between the red blood cell distribution width and primary angle-closure glaucoma: a potential for disease prediction.

The red blood cell distribution width (RDW) is a simple and inexpensive laboratory parameter that can be linked to oxidative stress, inflammation and microvascular flow resistance. For this research, we performed a large-sample case-control study to describe the relationships between the RDW and primary angle-closure glaucoma (PACG). A total of 1191 PACG patients (422 males and 769 females), who were divided into mild, moderate and severe PACG groups, and 982 healthy controls (344 males and 638 females) were recruited between January 2008 and June 2018.

Applications of Bioactive Ions in Bone Regeneration.

The repair of large bone defects remains a huge challenge for bone regenerative medicine. To meet this challenge, a number of bone substitutes have been developed over recent years to overcome the drawbacks of traditional autograft and allograft therapies. Thus, the improvement of the osteoinductive ability of these substitutes has become a major focus for research in the field of bone tissue engineering.

[Effect of overexpression of transcription factor Runx2 and Osterix on osteogenic differentiation of endothelial cells].

Purpose: To explore the effect of overexpression of Runx2 and Osterix (OSX) genes on osteogenic differentiation of human umbilical vein endothelial cells (HUVECs).

Methods: Overexpressed Runx2 and OSX lentiviral vectors were transfected into HUVECs respectively. The osteogenic potential of transfected cells was identified by alkaline phosphatase (ALP) staining and ALP activity.

Meta-analysis reveals gender difference in the association of liver cancer incidence and excess BMI.

Excess body weight has a positive association with risk of liver cancer, but the gender difference in the relationship between body mass index and liver cancer risk remains uncertainty. In this work, we performed meta-analysis for excess body weight and risk of liver cancer incidence to identify the gender difference. We searched the English-languages database and the Chinese literature databases to May 12, 2017.

Quantitative Proteomic Analysis of Rat Condylar Chondrocytes during Postnatal Development.

Objective: To investigate differentially expressed proteins in rat mandibular condylar cartilage (MCC) chondrocytes caused by initial mastication for short postnatal periods.

Methods: Four groups of protein samples were extracted from primary cultured rat MCC chondrocytes, harvested from eigthy postnatal SD rats aged 1,7,14 and 28 days, with twenty in each group. Total proteins were labelled with isobaric tags for relative and absolute quantification (iTRAQ) reagents.

Expansion of CD14(+)CD16(+) monocytes is related to acute leukemia.

Objective: Aim to investigate the proportion of CD14(+)CD16(+) monocytes and understand the pathogenesis of this monocyte subset in acute leukemia.

Methods: Flow cytometry was utilized to study the phenotype expression of CD14(+)CD16(+) monocytes and CD3(+) T lymphocytes in peripheral blood derived from patients with acute leukemia. All the data were analyzed by SPSS 13.

Non-coding regions of the Ebola virus genome contain indispensable phylogenetic and evolutionary information.

We compared the numbers of nucleotide substitutions occurring in the non-coding regions and coding regions of Ebola virus genomes and found that non-coding regions contain indispensable phylogenetic and evolutionary information. The omission of genetic data from non-coding regions can lead to unreliable phylogenies and inaccurate estimates of evolutionary parameters.

[Repair of calvarial defect using a tissue-engineered bone with simvastatin-loaded β-tricalcium phosphate scaffold and adipose derived stem cells in rabbits].

Purpose: The osteogenic-angiogenic differentiation effects of simvastatin (Sim) were explored on adipose tissue-derived stem cells (ASCs). A tissue-engineered bone with simvastatin loaded β-tricalcium phosphate (β-TCP) scaffold and ASCs was constructed to repair the calvarial defect in rabbits.

Methods: ASCs were obtained from the groin of rabbits.

Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats.

Type 1 diabetes mellitus (T1DM) is associated with a series of bone complications, which are still a great challenge in the clinic. Bone marrow stromal cells (BMSCs) are crucial to bone remodeling and are attractive candidates for tissue engineering. Hence, we aimed to investigate whether impaired functions of BMSCs play a role in the pathogenesis of bone complications associated with T1DM.

The microtensile bond strengths of four resin core materials and fiber posts and their micromorphologic characteristics.

Objective: To evaluate the morphologies of D.T. Light-Post (LP), JFP-II quartz (JQ), and JFP-II glass (JG) fiber posts and their microtensile bond strengths to light- or dual-cured resin core materials.

[Cloning and expression of human interleukin-32 and studies on its bioactivity].

Aim: To clone human interleukin-32(hIL-32) gene and express it in E.coli efficiently.

Methods: The gene of hIL-32 was amplified by RT-PCR from human peripheral blood mononuclear cells (PBMC) which stimulated with Con A for 60 h.

[Effect of HPV 16 E5 on E6 and E7 gene induced human immortalized oral epithelial cell].

Purpose: To investigate the influence of HPV16 E5 on E6 and E7 gene in human oral immortalized epithelial cells (HIOEC).

Methods: The pLEGFP-E5 was transferred into HIOEC cell; Deletion mutation expression vectors were constructed and transferred into HIOEC; RT-PCR was used to detect the gene expression in HIOEC; The expression of E6 and E7 mRNA was detected by real time-PCR;Cell proliferation of HIOEC was evaluated by MTT. All statistical analysis was performed by using SPSS 13.

[Construction and detection of gene expression vector of the Von Hippel-Lindau in bone marrow stromal cells].

Purpose: To construct siRNA-VHL expression vector and detect the effect of VHL gene interference on BMSCs.

Methods: According to the dog's VHL gene sequences, four pairs of siRNA oligo were designed and synthesized. Using vector cloning kit reorganization, four pairs of double-stranded siRNA were inserted into the expression vector (pcDNA™ 6.

[The experimental study on porous calcium phosphate cement with bone marrow stromal cells for bone tissue engineering].

Objective: To observe the biocompatibility of new biomaterials porous calcium phosphate (CPC) and ectopic bone formation of CPC with bone marrow stromal cells (BMSCs).

Methods: The BMSCs were cultured from Beagle dog and combined with the porous CPC with the best concentration after transfect green fluorescent protein (GFP). The adhesion and growth of BMSCs on CPC were observed under inversion, fluorescence and scanning electron microscopy.

[Fabrication of titanium dioxide nanotube array and effects of its osteoblast proliferation and alkaline phosphatase activity].

Objective: To investigate preparation parameters of TiO2 nanotube layer with anodization, and to evaluate the osteoblast activity on TiO2 nanotube layer in vitro.

Methods: Titanium dioxide nanotube layer was grown using anodization method. TiO2 nanotube layers with different structure were obtained by controlling the voltage and time of anodization and rinsing process after anodization.

Evidence for calcifying nanoparticles in gingival crevicular fluid and dental calculus in periodontitis.

Background: Calcifying nanoparticles (CNPs), also known as nanobacteria, can produce carbonate apatite on their cell walls and initiate pathologic calcification. The objective of this study was to determine whether CNPs are present in the gingival crevicular fluid (GCF) from subjects with periodontal disease and whether they can induce the pathologic calcification of primary cultured human gingival epithelial cells.

Methods: GCF and dental calculus samples were collected from 10 subjects with gingivitis and 10 subjects with chronic periodontitis.

Sonic hedgehog alleviates the inhibitory effects of high glucose on the osteoblastic differentiation of bone marrow stromal cells.

To assess the influence of high extracellular glucose levels on the osteogenic differentiation of bone marrow stromal cells (BMSCs) and to determine if Sonic hedgehog (Shh) protein can alleviate those effects. BMSCs were incubated with NG (normal glucose), NG+Shh (200 ng/ml Shh in normal glucose), NG+Shh+Gan (200 ng/ml Shh and 5 micromol/L GANT61 in normal glucose), HG (high glucose), HG+Shh (200 ng/ml Shh in high glucose), and HG+Shh+Gan (200 ng/ml Shh and 5 micromol/L GANT61 in high glucose). The expression levels of Shh signaling pathway genes Patched 1 (PTCH1) and osteogenesis-related genes were tested, which included bone morphogenetic protein 4 (BMP4), runt-related transcription factor 2 (Runx2), and osteopontin (OPN).