Architecture of photosystem I-light-harvesting complex from the eukaryotic filamentous yellow-green alga Tribonema minus.

Eukaryotic photosystem I (PSI) is a multi-subunit pigment-protein supercomplex that consists of a core complex and multiple peripheral light-harvesting complexes I (LHCIs), which increases the light absorption capacity of the core complex. Throughout the evolution of oxygenic photoautotrophs, the core subunits of PSI have remained highly conserved, while LHCIs exhibit significant variability, presumably to adapt to diverse environments. This study presents a 2.

A multicenter study on the diagnostic value of ankle brachial index combined with pulse volume wave parameters for peripheral arterial disease.

Objective: To evaluate the significance of incorporating all pulse volume wave parameters, such as the inter-arm systolic blood pressure disparity, inter-leg systolic blood pressure difference, proportion of mean arterial pressure, and upstroke time, into the ankle-brachial index for the detection of peripheral arterial disease.

Methods: This multicenter cross-sectional investigation, conducted across five tertiary medical institutions, enrolled 1,156 participants. Hemodynamic parameters including blood pressure and pulse volume were systematically assessed utilizing an OMRON BP-203RPEIII arterial stiffness analyzer.

Deciphering the lipid composition and metabolic patterns of cashew apple: A comprehensive lipidomic analysis.

To thoroughly analysis the lipid composition and regularities of distribution in different cashew apple varieties, the fatty acid composition and lipid composition of cashew apples were separated and identified by ultra-high performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry, and the overall differences and metabolic pathways of lipids in eight cashew apples were analyzed by OPLS-DA and metabolomics. The results showed that cashew apples were composed of 17 fatty acids (oleic acid and palmitic acid, etc.) and 320 lipids in eight cashew apples, among which 190 lipid compounds with VIP value greater than 1 were screened out, and the lipid compounds in different cashew apples were quite different.

Application analysis of a new pre-localization series algorithm for differentiating wide QRS complex tachycardia.

Background: Accurate diagnosis of wide QRS complex tachycardia (WCT) holds significant clinical value for further diagnosis and treatment.

Objective: To evaluate a new WCT differentiation algorithm, defined as the pre-localization series algorithm, which includes the series process for differentiating ventricular tachycardia (VT) from supraventricular tachycardia (SVT) and differentiating VT from preexcited tachycardia (PXT).

Methods: A retrospective analysis of electrocardiograms (ECGs) from 185 patients with WCT was conducted using the pre-localization single-process algorithm (differentiating VT from SVT without differentiating VT from PXT), the pre-localization series algorithm, the limb lead algorithm, and the Basel algorithm.

PCSK9 Promotes LDLR Degradation by Preventing SNX17-Mediated LDLR Recycling.

Background: Low-density lipoprotein (LDL) is internalized into cells mainly through LDLR (LDL receptor)-mediated endocytosis. In an acidic endosome, LDLR is released from LDL and recycles back to the cell surface, whereas LDL is left in the endosome and degraded in the lysosome. Circulating PCSK9 (proprotein convertase subtilisin/kexin 9) binds with LDLR and is internalized into the endosome, similar to LDL.

Effective discrimination of wide QRS complex tachycardia with a new algorithm - the Prelocalization Series Algorithm.

Background: Electrocardiogram (ECG) plays a crucial role in the correct diagnosis of wide QRS complex tachycardia (WCT). Objective To evaluate the diagnostic value of a new WCT discrimination algorithm, herein referred to as the Prelocalization Series Algorithm.

Methods: A retrospective analysis of 181 ECGs from WCT patients was conducted using the Prelocalization Series Algorithm, Brugada Series Algorithm, and Vereckei Series Algorithm.

Integrated analyses of characterization and transcriptome reveal the adaptive response mechanism of Bacillus cereus FCHN 7-1 in cadmium adsorption.

Cadmium (Cd) pollution is a pressing environmental issue that seriously threatens human health. In recent years, microbial extracellular polymeric substances (EPS) have been developed as an eco-friendly and effective solution for heavy metal bioremediation. In this study, Bacillus cereus FCHN 7-1, which was isolated from highly Cd-polluted soils in Hunan Province, China, has strong resistance to Cd and excellent Cd adsorptive capacity.

Nicotine-Induced Transient Activation of Monocytes Facilitates Immunosuppressive Macrophage Polarization that Restrains T Helper 17 Cell Expansion.

Macrophages in smoking environment exhibit a distinct immunosuppressive phenotype, but the mechanisms that allow nicotine to "educate" macrophages are incompletely understood. Here, we identified that nicotine transiently activates and subsequently deactivates monocytes, leading to reduced anti-infective capability of macrophages. This deactivation results in a suppression of IL-17-producing cell expansion through decreased IL-1β production.

Replacing traditional coffee appraisers with inductively coupled plasma - mass spectrometry (ICP-MS): From manual sensory evaluation to scientific analysis.

Scientific development provides opportunities to replace many traditional manual methods to achieve more accurate results and higher efficiency. To scientifically ascertain the geographical origin of coffee, this study develops a method for the rapid determination of 16 inorganic elements in coffee using microwave digestion combined with the ICP-MS internal standard method. Principal component analysis (PCA), Fisher discriminant analysis (FDA), and Partial least squares discriminant analysis (PLS-DA) are employed to analyze 40 coffee samples from three production areas of Pu'er, Baoshan, and Wanning.

Rare-Earth Metalloligands for LowValent Cobalt Complexes: Fine Electronic Tuning Co→RE Dative Interactions.

Rare-earth metalloligand supported low-valent cobalt complexes were synthesized by utilizing a small-sized heptadentate phosphinomethylamine H and a large-sized arene-anchored hexadentate phosphinomethylamine H ligand precursors. The RE(III)-Co(-I)-N (RE = Sc, Lu, Y, Gd, La) complexes containing rare-earth metals including the smallest Sc and largest La were characterized by multinuclear NMR spectroscopy, X-ray diffraction analysis, electrochemistry, and computational studies. The Co(-I)→RE(III) dative interactions were all polarized with major contributions from the 3d orbital of the cobalt center, which was slightly affected by the identity of rare-earth metalloligands.

A scandium metalloligand supported Ni(0) complex with a heterobimetallocycle: versatile reactivity with unsaturated bonds.

A N-bridged tetranuclear Sc(III)-Ni(0) complex featuring a Ni → Sc interaction and a 4-membered [Sc-N-C-Ni] ring was synthesized and characterized. Bimetallic reactivity was demonstrated reactions with a series of unsaturated compounds containing NC, CN, CC, CO and NN bonds.

ZIF-67-Derived Co/N-Doped Carbon-Functionalized MXene for Enhanced Electrochemical Sensing of Carbendazim.

Herein, ZIF-67-derived Co and N-doped carbon (Co/NC) particle-modified multilayer MXene (MXene@Co/NC) was developed as remarkable electrode material for carbendazim (CBZ) detection. MXene as a substrate provides an excellent conductive framework and plentiful accessibility sites. Co/NC particles embedding in MXene can not only prevent the interlayer stacking of MXene but also contribute a great deal of metal catalytic active sites and finally improve the adsorption and catalytic properties of the composite.

Structural insights into the assembly and energy transfer of the Lhcb9-dependent photosystem I from moss Physcomitrium patens.

In plants and green algae, light-harvesting complexes I and II (LHCI and LHCII) constitute the antennae of photosystem I (PSI), thus effectively increasing the cross-section of the PSI core. The moss Physcomitrium patens (P. patens) represents a well-studied primary land-dwelling photosynthetic autotroph branching from the common ancestor of green algae and land plants at the early stage of evolution.

Structural, Spectroscopic, and Bonding Analyses of La(III)/Ce(III)-Tetrel Ate-Complexes.

In comparison with the research of transition-metal-tetrel complexes, the chemistry of lanthanide tetrel complexes, especially for these bearing heavier tetrel element ligands, is still relatively underexplored. In this research, K[CpLn(III)CHPh], [(DME)Li][CpLn(III)GePh], and [(DME)Li][CpLn(III)SnPh] [Ln(III) = La(III), Ce(III)] have been synthesized by reacting [(DME)Na][CpLa(-Cl)LaCp] or CpCe(THF) with alkali metal alkyl, germyl, and stannyl reagents. Additionally, [(DME)Li][CpCe(III)SnPh] is the first example of Ce(III)-Sn bond containing complex.

Dynamic Regulation of the Light-Harvesting System through State Transitions in Land Plants and Green Algae.

Photosynthesis constitutes the only known natural process that captures the solar energy to convert carbon dioxide and water into biomass. The primary reactions of photosynthesis are catalyzed by the photosystem II (PSII) and photosystem I (PSI) complexes. Both photosystems associate with antennae complexes whose main function is to increase the light-harvesting capability of the core.

Dinitrogen Complexes of Cobalt(-I) Supported by Rare-Earth Metal-Based Metalloligands.

Sequential reactions of heptadentate phosphinoamine LH with rare-earth metal tris-alkyl precursor (MeSiCH)Ln(THF) (Ln = Sc, Lu, Yb, Y, Gd) and a low-valent cobalt complex (PhP)CoI afforded rare-earth metal-supported cobalt iodide complexes. Reduction of these iodide complexes under N allowed the isolation of the first series of dinitrogen complexes of Co(-I) featuring dative Co(-I) → Ln (Ln = Sc, Lu, Yb, Y, Gd) bonding interactions. These compounds were characterized by multinuclear NMR spectroscopy, X-ray diffraction analysis, electrochemistry, and computational studies.

Structural insights into photosynthetic cyclic electron transport.

During photosynthesis, light energy is utilized to drive sophisticated biochemical chains of electron transfers, converting solar energy into chemical energy that feeds most life on earth. Cyclic electron transfer/flow (CET/CEF) plays an essential role in efficient photosynthesis, as it balances the ATP/NADPH ratio required in various regulatory and metabolic pathways. Photosystem I, cytochrome bf, and NADH dehydrogenase (NDH) are large multisubunit protein complexes embedded in the thylakoid membrane of the chloroplast and key players in NDH-dependent CEF pathway.

Responses of soil microbial communities to concentration gradients of antibiotic residues in typical greenhouse vegetable soils.

To explore the responses of soil microbial communities to concentration gradients of antibiotic residues in soil, 32 soil samples were collected from a typical greenhouse vegetable production base in Northern China in 2019. The total concentrations of 26 antibiotic residues in these soil samples was 83.24-4237.

Early Lanthanide(III) Ate Complexes Featuring Ln-Si Bonds (Ln = La, Ce): Synthesis, Structural Characterization, and Bonding Analysis.

While research on lanthanide (Ln) complexes with silyl ligands is receiving growing attention, significantly unbalanced efforts have been devoted to different Ln elements. In comparison with the intense investigations on Ln elements such as Sm and Yb, the chemistry of silyl lanthanum and cerium complexes is much slower to develop, and no solid-state structure of a silyl lanthanum complex has been reported so far. In this research, four types of ate complexes, including [(DME)Li][CpLnSi(H)Mes], [(18-crown-6)K][CpLnSi(CH)Ph], [(DME)Li][CpLnSiPh], and [(12-crown-4)Na] [CpLnSi(Ph)Si(H)Ph] (Ln = La, Ce), were synthesized by reacting [(DME)Na][CpLa(μ-Cl)LaCp] or CpCe(THF) with alkali metal silanides.

Structural and Biochemical Analysis Reveals Catalytic Mechanism of Fucoidan Lyase from sp. SA-0082.

Fucoidans represent a type of polyanionic fucose-containing sulfated polysaccharides (FCSPs) that are cleaved by fucoidan-degrading enzymes, producing low-molecular-weight fucoidans with multiple biological activities suitable for pharmacological use. Most of the reported fucoidan-degrading enzymes are glycoside hydrolases, which have been well studied for their structures and catalytic mechanisms. Little is known, however, about the rarer fucoidan lyases, primarily due to the lack of structural information.

Structure of Arabidopsis SOQ1 lumenal region unveils C-terminal domain essential for negative regulation of photoprotective qH.

Non-photochemical quenching (NPQ) plays an important role for phototrophs in decreasing photo-oxidative damage. qH is a sustained form of NPQ and depends on the plastid lipocalin (LCNP). A thylakoid membrane-anchored protein SUPPRESSOR OF QUENCHING1 (SOQ1) prevents qH formation by inhibiting LCNP.

Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana.

Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like (NDH) complex that contains at least 29 protein subunits and associates with photosystem I (PSI) to form the NDH-PSI supercomplex. Here, we report the 3.

Structural basis of LhcbM5-mediated state transitions in green algae.

In green algae and plants, state transitions serve as a short-term light-acclimation process in the regulation of the light-harvesting capacity of photosystems I and II (PSI and PSII, respectively). During the process, a portion of light-harvesting complex II (LHCII) is phosphorylated, dissociated from PSII and binds with PSI to form the supercomplex PSI-LHCI-LHCII. Here, we report high-resolution structures of PSI-LHCI-LHCII from Chlamydomonas reinhardtii, revealing the mechanism of assembly between the PSI-LHCI complex and two phosphorylated LHCII trimers containing all four types of LhcbM protein.

Assembly of eukaryotic photosystem II with diverse light-harvesting antennas.

Photosystem II (PSII) catalyzes the light-driven oxygen-evolving reaction via its catalytic core and peripheral light-harvesting antennas. Oxyphototrophs have evolved diverse antenna systems, enabling them to adapt to different habitats. Recently, high-resolution structures of PSII-antenna supercomplexes from the green lineage (higher plants and green algae) and the red lineage (diatoms) were solved.