Duloxetine inhibits voltage-dependent K channels in rabbit coronary arterial smooth muscle: Electrophysiological evidence and mechanistic insights.

Duloxetine, a serotonin-norepinephrine reuptake inhibitor, has been associated with increased blood pressure and other vascular side effects. However, the mechanisms underlying these effects remain poorly understood. Voltage-dependent K (Kv) channels are key regulators of membrane potential and vascular tone in arterial smooth muscle cells.

Osmotic stress-induced CsRCI2E endosomal trafficking modulates CsPIP2 aquaporins at the plasma membrane in Camelina sativa.

Rare Cold Inducible 2s (RCI2s) are membrane-associated proteolipids dynamically trafficking between the plasma membrane (PM) and the endomembrane system. Their expression is upregulated in response to abiotic stresses, including cold, heat, drought, and salinity, contributing to plant stress tolerance. CsRCI2E interacts with the water transport protein CsPIP2; 1, reducing its abundance at the PM under NaCl-induced stress.

Radiotherapy quality assurance program of ongoing clinical trial using stereotactic ablative radiation therapy for recurrent ovarian cancer (SABR-ROC): a dummy run study of a prospective, randomized, multicenter phase III trial (KGOG 3064/KROG 2204).

Background: Recurrent ovarian cancer is often treated with chemotherapy, but many patients experience multiple recurrences with progressively shorter intervals and poorer prognosis. Repeated chemotherapy reduces patients' quality of life. Stereotactic Ablative Radiation Therapy for Recurrent Ovarian Cancer (SABR-ROC) (KGOG3064/KROG 2204) is an ongoing trial investigating the clinical efficacy of stereotactic ablative radiation therapy (SABR) for recurrent ovarian cancer.

The Second-Generation Antipsychotic Perospirone Inhibits Voltage-Gated K Channels in Coronary Arterial Smooth Muscle Cells.

Perospirone is a second-generation antipsychotic classified as a serotonin-dopamine antagonist that is primarily used to treat schizophrenia and bipolar disorder. While its therapeutic effects have been attributed to D and 5-HT receptor antagonism and partial 5-HT agonism, its potential interactions with ion channels remain unexplored. In this study, we investigated the effects of perospirone on vascular voltage-gated K (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells.

Through-Layer Contact with Encapsulated Graphene Using One-Step Laser Scribing.

Encapsulated graphene plays a key role in enabling stable and functional operation of next-generation electronic and sensing devices. However, establishing electrical contact with encapsulated graphene remains a significant challenge because of the need for separate etching and metal deposition steps, which require complex multistep fabrication processes. This paper introduces a scalable one-step method for forming electrical contacts with encapsulated graphene using CO laser scribing.

Surgery plus post-operative radiotherapy versus definitive chemoradiotherapy in locally advanced endocervical adenocarcinoma.

Objective: This study evaluated whether treatment outcomes for endocervical adenocarcinoma differ according to treatment modality (surgery plus post-operative radiotherapy versus definitive chemoradiotherapy) and human papillomavirus (HPV) status.

Methods: We retrospectively analyzed 105 patients with clinical stage IIB to IIIC endocervical adenocarcinoma, classified according to the 2018 International Federation of Gynecology and Obstetrics staging system, who were treated with surgery plus post-operative radiotherapy or definitive chemoradiotherapy at a single institution between 2011 and 2022. HPV status was determined based on the 2020 World Health Organization classification.

Exploring the Therapeutic Potential of : A Network Pharmacology and Molecular Docking Analysis Across Multiple Diseases.

(RG), a fundamental herb in traditional Chinese medicine belonging to the Orobanchaceae family, has been widely used for centuries due to its diverse therapeutic properties, including promoting blood circulation, enhancing immunity, managing diabetes, reducing inflammation, and supporting kidney function. Despite its traditional significance, scientific studies on RG's therapeutic mechanisms remain limited, and its underlying pharmacological pathways are not extensively elucidated. This study employed network pharmacology and molecular docking to identify RG's active compounds and investigate their therapeutic potential in allergy, anemia, diabetes, and menopause.

Image processing with Optical matrix vector multipliers implemented for encoding and decoding tasks.

This study introduces an optical neural network (ONN)-based autoencoder for efficient image processing, utilizing specialized optical matrix-vector multipliers for both encoding and decoding tasks. To address the challenges in efficient decoding, we propose a method that optimizes output processing through scalar multiplications, enhancing performance in generating higher-dimensional outputs. By employing on-system iterative tuning, we mitigate hardware imperfections and noise, progressively improving image reconstruction accuracy to near-digital quality.

Postoperative conventional versus hypofractionated intensity-modulated radiation therapy with concurrent chemotherapy in cervical cancer: a prospective multicenter randomized phase III trial (POHIM_P3 trial).

Background: For patients with high-risk factors such as pelvic lymph node metastasis, positive surgical margins, or parametrial involvement, concurrent chemoradiotherapy (CCRT) with whole-pelvic radiotherapy significantly improves survival outcomes. Hypofractionated radiation therapy, which delivers higher radiation doses over fewer sessions, enhances tumor control but raises concerns about increased normal tissue toxicity. A recent Korean phase II study (POHIM-CCRT) evaluated the safety of hypofractionated intensity-modulated radiation therapy (IMRT), delivering 40 Gy in 16 fractions with weekly cisplatin following radical surgery.

In-Situ Vertical-Contact Engineering of Laser-Induced Graphene Nanotips for Ultra-Sensitive Humidity Sensors.

Advancements in laser-induced graphene (LIG) technology enables the streamlined fabrication of 3D porous graphene-based humidity sensors. However, conventional LIG-based humidity sensors employing lateral-contact configurations often exhibit limited responsivity, owing to their partially exposed structures and dominant subsurface current pathways. This study presents a novel vertical-contact architecture that utilizes vertically aligned LIG nanotips for ultra-sensitive humidity detection.

Gut microbiota dysbiosis induced by brain tumors modulates the efficacy of immunotherapy.

Although the influence of gut microbiota on various tumors has gained recognition, the intricate mechanisms underlying its effects on brain tumors remain largely unexplored. Our study aims to discover crucial gut microbiota that significantly bolster the immune response against brain tumors. Employing a murine model, we utilize 16S rRNA sequencing to analyze the evolution of gut microbiota alongside tumor progression, and we intervene with dietary adjustments using tryptophan to restore microbiota alterations.

Placental inflammation-driven T cell memory formation promotes allergic responses in offspring via endogenous glucocorticoids.

Maternal exposure to environmental change during pregnancy is a critical determinant of offspring health and disease. Previous epidemiological studies have reported that maternal inflammation is linked to an increased incidence of postnatal allergy in offspring, although the underlying mechanisms remain largely unexplored. In this study, we employed a lipopolysaccharide-induced maternal inflammation murine model and found that offspring from dams with maternal immune activation (MIA) exhibited heightened allergic responses to house dust mite allergen.

Oncogenic function of growth arrest-specific transcript 5 by competing with miR-423-3p to regulate SMARCA4 in hepatocellular carcinoma.

Long noncoding RNA growth arrest-specific transcript 5 (GAS5) has been identified as a tumor suppressor due to its downregulation in several cancers. However, our comprehensive analyses revealed aberrant overexpression of GAS5 in various cancers, with a direct association with SMARCA4 in hepatocellular carcinoma (HCC). Differential expression analyses were conducted using publicly available transcriptome datasets.

Postoperative hypofractionated intensity-modulated radiation therapy in cervical cancer: prospective phase 2 trial (POHIM_RT study).

Objective: This study evaluates the safety and efficacy of hypofractionated intensity-modulated radiation therapy (IMRT) following radical hysterectomy.

Methods: This phase II, non-randomized multicenter trial included 61 patients with cervical cancer who were eligible for adjuvant RT after radical hysterectomy. Participants received 40 Gy in 16 fractions using IMRT to whole pelvis.

Gut dysbiosis from high-salt diet promotes glioma via propionate-mediated TGF-β activation.

The purpose of this study is to investigate the impact of a high-salt diet (HSD), which is commonly found in Western countries, on the progression of glioma. Our research shows that the alterations in gut microbiota caused by an HSD facilitated the development of glioma. Mice fed an HSD have elevated levels of intestinal propionate, which accelerated the growth of glioma cells.

Investigating Natural Product Inhibitors of IKKα: Insights from Integrative In Silico and Experimental Validation.

Nuclear factor-κB (NF-κB) signaling plays a pivotal role in regulating immune responses and is strongly implicated in cancer progression and inflammation-related diseases. The inhibitory κB kinases (IKKs), particularly IKKα, are central to modulating NF-κB activity, with distinct roles in the canonical and non-canonical signaling pathways. This study investigates the potential of selectively targeting IKKα to develop novel therapeutic strategies.

Identification of single nucleotide polymorphisms and candidate genes associated with fiber content in sweetpotato (Ipomoea batatas (L.) Lam.) through a genome-wide association study.

Background: Sweetpotato (Ipomoea batatas (L.) Lam.) is an essential root crop with several nutritional benefits, including high dietary fiber content.

Understanding Immune Cell Adaptation to Tumor Hypoxia for Maximized Therapeutic Efficacy of Immunotherapy: Biology and Non-Invasive Imaging Application.

It is extensively documented that tumor hypoxia contributes to the failure of chemotherapy and radiotherapy. Recent evidence suggest hypoxia is also closely involved in the resistance to immunotherapy. In this review, we highlight how immune cells that are essential for the maximized immunotherapy efficacy, including cytotoxic T cells, dendritic cells, and natural killer cells can adapt to tumor hypoxia.

The sodium-glucose cotransporter 2 inhibitor tofogliflozin induces vasodilation of rabbit femoral artery by activating Kv channels, the SERCA pump, and the sGC/cGMP pathway.

Tofogliflozin is a sodium-glucose cotransporter 2 inhibitor widely used to treat type 2 diabetes mellitus, but it also exhibits cardio-protective effects. This study investigated the vasodilatory action of tofogliflozin using rabbit femoral artery rings pre-contracted with phenylephrine (1 μM). The results showed the concentration-dependent induction of vasodilation by tofogliflozin, a response that remained unchanged following endothelial removal, pretreatment with the nitric oxide synthase inhibitor L-NAME (100 μM), or the inhibition of low- and intermediate-conductance Ca-activated K channels using apamin (1 μM) in combination with TRAM-34 (1 μM).

Significance of HPV status on tumor response and treatment outcomes in endocervical adenocarcinoma treated with definitive chemoradiotherapy: a retrospective study.

Objective: We aimed to compare tumor response and treatment outcomes between human papillomavirus (HPV)-associated (HPVA) and HPV-independent (HPVI) endocervical adenocarcinomas (ADCs) treated with definitive concurrent chemoradiotherapy (CCRT) and to identify prognostic factors.

Methods: We conducted a retrospective review of 40 patients with endocervical ADCs treated with definitive CCRT (stages I-IVA) between 2011 and 2022. Based on pathological review the cases were categorized as HPVA or HPVI ADCs.

Clinical implications of deep learning based image analysis of whole radical prostatectomy specimens.

Prostate cancer (PCa) diagnosis faces significant challenges due to its complex pathological characteristics and insufficient pathologist resources. While deep learning-based image analysis (DLIA) shows promise in enhancing diagnostic accuracy, its application to radical prostatectomy (RP) specimens remains underexplored. In this study, we evaluated the clinical feasibility and prognostic value of a DLIA algorithm for Gleason grading and tumor quantification on whole RP specimens.

A perspective on reoperations after surgical treatments of degenerative lumbar diseases.

Clinical data including reoperation rates are comparable for lumbar patients after decompression-alone or decompression-with-fusion surgeries. However, there could exist different mechanisms causing complications after the two surgeries. We discussed research and technology development perspectives for improvement of the surgeries using published clinical outcome data.

Predicting 30-day mortality with routine blood tests in patients undergoing palliative radiation therapy: A comparison of logistic regression and gradient boosting models.

Purpose: This study aimed to estimate the 30-day mortality (30D_M) and compare models for 30D_M prediction in patients undergoing palliative radiation therapy (RT).

Materials And Methods: Data from 3,756 patients who underwent palliative RT between 2018 and 2020 at two institutions were retrospectively reviewed. From one institution, 3,315 patients were randomly assigned to the training (N = 2,652) and internal validation (N = 663) cohorts.

Prevalence of asymptomatic malaria in high- and low-transmission areas of Tanzania: The role of asymptomatic carriers in malaria persistence and the need for targeted surveillance and control efforts.

As many countries implement different programs aimed at eliminating malaria, attention should be given to asymptomatic carriers that may interrupt the progress. This was a community-based cross-sectional study conducted in Tanzania from December 2022 to July 2023 within 4 villages from each of the 3 regions, Geita and Kigoma, which are high malaria transmission, and Arusha, which is low transmission. Malaria was diagnosed in asymptomatic individuals aged 1 year and older using the malaria rapid diagnostic test and light microscope.

Monotropein resists atherosclerosis by reducing inflammation, oxidative stress, and abnormal proliferation and migration of vascular smooth muscle cells.

Monotropein is a compound classified into iridoid which is found in herbaceous plants Morindae officinalis. It possesses anti-inflammatory, antioxidant, and anti-osteoarthritic activities. Previous study indicates that monotropein may have the potential to combat cardiovascular disease, although the related mechanism remains unclear.