Epidemiology and genetic characterization of (X4)-positive and isolated from raw meat in Chengdu City, China.

The rapid spread of mobile tigecycline resistance presents a significant public health threat, particularly with the increasing prevalence of (X4)-positive Enterobacterales across various species. This study aimed to investigate the epidemic features and transmission dynamics of (X4)-positive (. ) through the analysis of 206 raw meats, including pork ( = 182), beef ( = 16), duck ( = 5), and chicken ( = 3).

The co-occurrence of tet(X4) and tmexCD2-toprJ2 mediated tigecycline resistance in Raoultella ornithinolytica.

Objectives: This study aimed to characterize the co-occurrence of the tigecycline resistance determinants tet(X4) and tmexCD2-toprJ2 in a Raoultella ornithinolytica isolate collected from a pig rectal swab at the slaughterhouse.

Methods: The R. ornithinolytica isolate WS60 was subjected to antimicrobial susceptibility testing.

Tracing the evolution: the rise of Thompson co-resistant to clinically important antibiotics in China, 1997-2020.

As clinical serovar Thompson (. Thompson) emerged among the top ten prevalent serovars in China, understanding the distribution and origin of its multidrug-resistant (MDR) strains becomes imperative. This study employed antimicrobial susceptibility testing, whole-genome sequencing, and bioinformatics analysis to investigate the prevalence and genomic profiles of clinically important .

Comparative efficacy of anaerobic digestion systems in removing antimicrobial resistance genes from swine wastewater.

Swine farm wastewater is a major reservoir of antimicrobial resistance genes (ARGs). Anaerobic digestion (AD), widely implemented in farms, has been extensively studied for ARG removal. However, a comparative study on ARG removal efficiency across the four principal AD systems - up-flow anaerobic sludge blanket (UASB), continuous stirred tank reactor (CSTR), buried biogas digester (BBD), and septic tank (SPT) - is lacking.

Limited transmission of carbapenem-resistant between animals and humans: a study in Qingdao.

Despite no carbapenem use in food animals, carbapenem-resistant (CRKP) perseveres within food animals, rising significant concerns regarding public health risks originating from these non-clinical reservoirs. To investigate the potential link between CRKP in food animals and its infections in humans, we conducted a cross-sectional study encompassing human clinical, meat products, and farm animals, in Qingdao city, Shandong province, China. We observed a relatively higher presence of CRKP among hospital inpatients (7.

Transmission of in Enterobacteriaceae among animals, food and human.

Despite carbapenems not being used in animals, carbapenem-resistant Enterobacterales (CRE), particularly New Delhi metallo-β-lactamase-producing CRE (NDM-CRE), are prevalent in livestock. Concurrently, the incidence of human infections caused by NDM-CRE is rising, particularly in children. Although a positive association between livestock production and human NDM-CRE infections at the national level was identified, the evidence of direct transmission of NDM originating from livestock to humans remains largely unknown.

MC-PRPA-HLFIA Cascade Detection System for Point-of-Care Testing Pan-Drug-Resistant Genes in Urinary Tract Infection Samples.

Recently, urinary tract infection (UTI) triggered by bacteria carrying pan-drug-resistant genes, including carbapenem resistance gene and , colistin resistance gene , and (X) for tigecycline resistance, have been reported, posing a serious challenge to the treatment of clinical UTI. Therefore, point-of-care (POC) detection of these genes in UTI samples without the need for pre-culturing is urgently needed. Based on PEG 200-enhanced recombinase polymerase amplification (RPA) and a refined Chelex-100 lysis method with HRP-catalyzed lateral flow immunoassay (LFIA), we developed an MCL-PRPA-HLFIA cascade assay system for detecting these genes in UTI samples.

Aeromonas spp. from hospital sewage act as a reservoir of genes resistant to last-line antibiotics.

Background: Aeromonas species are opportunistic pathogens distributed widely in the ecosystem. They are known to be capable of acquiring antibiotic resistance genes, including those encoding proteins against last-line antibiotics, such as the tmexCD-toprJ, mcr and carbapenemase genes. We investigated the genomic and phenotypic characteristics of tmexCD-toprJ-positive Aeromonas strains collected from human, animals, and water samples, particularly those from hospital wastewater in China.

Rapid On-Site Detection of Extensively Drug-Resistant Genes in via Enhanced Recombinase Polymerase Amplification and Lateral Flow Biosensor.

The widespread emergence of transferable extensively drug-resistant (XDR) genes, including and for carbapenem resistance, for colistin resistance, and (X4) and (X6) for tigecycline resistance, in poses a major threat to public health. Thus, rapid on-site detection of these XDR genes is urgently needed. We developed a cascade system with a unitary polyethylene glycol (PEG) 200-enhanced recombinase polymerase amplification (RPA) as the core, combined with a modified Chelex-100 lysis method and a horseradish peroxidase (HRP)-catalyzed lateral flow immunoassay (LFIA) biosensor, to accurately detect these genes in .

Spreading of -Carrying Plasmids among Staphylococci from Humans and Animals.

The multidrug resistance gene mediates resistance to multiple antimicrobial agents, including linezolid. Plasmids are the preferred vector for the dissemination of . However, the presence and transmission of -carrying plasmids among staphylococci from humans and animals have rarely been studied.

Fecal Carriage of Harboring the (X4)-IncX1 Plasmid from a Tertiary Class-A Hospital in Beijing, China.

The emergence of the mobile tigecycline-resistance gene, (X4), poses a significant threat to public health. To investigate the prevalence and genetic characteristics of the (X4)-positive in humans, 1101 human stool samples were collected from a tertiary class-A hospital in Beijing, China, in 2019. Eight isolates that were positive for (X4) were identified from clinical departments of oncology ( = 3), hepatology ( = 2), nephrology ( = 1), urology ( = 1), and general surgery ( = 1).

Clonal relationship of tet(X4)-positive Escherichia coli ST761 isolates between animals and humans.

Objectives: To characterize the relationship of tet(X4)-positive isolates from different hosts and environments.

Methods: PCR and MALDI-TOF MS were used to identify the tet(X4)-positive isolates. The MICs of 13 antimicrobial agents were determined by broth microdilution.

Structural diversity of the ISCR2-mediated rolling-cycle transferable unit carrying tet(X4).

Background: Mobile tigecycline-resistance gene tet(X) variants have emerged as diverse pathogens from animal, human as well as their associated environments, which could potentially threaten public health. The insertion sequence, ISCR2, carries tet(X4) for horizontal transfer by rolling-cycle (RC) transposition. However, the diversity of ISCR2 and tet(X4) isolated from different sources is largely unknown.

Presence of Mobile Tigecycline Resistance Gene (X4) in Clinical Klebsiella pneumoniae.

The recently emerged plasmid-mediated tigecycline resistance gene (X4) has mainly been detected in Escherichia coli but never in Klebsiella pneumoniae. Herein, we identified a clinical K. pneumoniae isolate that harbored the (X4) gene located on a non-self-transferable IncFII-type plasmid, which could be cotransferred with a conjugative plasmid to E.

Abundance of tigecycline resistance genes and association with antibiotic residues in Chinese livestock farms.

The discovery of plasmid-mediated tet(X) variants and efflux pump gene tmexCD1-toprJ1 conferring bacteria resistance to tigecycline has compromised glycylcycline as the last line of defense against infection, which poses serious threat to public health. Herein, real-time quantitative PCR was used to detect the abundance of seven tigecycline resistance genes (TRGs), including six tet(X) variants and tmexCD1-toprJ1, and insertion sequences ISCR2 and IS26. Then, the concentrations of nine antibiotics were quantified in fecal samples collected from 157 livestock farms in four Chinese provinces.

A Novel Transposon, Tn, Mediated Transfer of Variant in ESBL-Producing .

Purpose: The aim of this study was to determine the prevalence and transmission mechanism of in spp. isolated from chicken cloaca.

Materials And Methods: w55 was isolated from chicken in 2008.

Fitness Cost of -Carrying p3R-IncX3 Plasmids in Wild-Type NDM-Free .

The wide dissemination of New Delhi metallo-β-lactamase genes () has resulted in the treatment failure of most available β-lactam antibiotics, with IncX3-type -carrying plasmids recognised as having spread worldwide. In China, bacteria carrying these plasmids are increasingly being detected from diverse samples, including hospitals, communities, livestock and poultry, and the environment, suggesting that IncX3 plasmids are becoming a vital vehicle for dissemination. To elucidate the fitness cost of these plasmids on the bacterial host, we collected -negative strains from different sources and tested their ability to acquire the -harboring p3R-IncX3 plasmid.

Identification of the novel tigecycline resistance gene tet(X6) and its variants in Myroides, Acinetobacter and Proteus of food animal origin.

Objectives: To report a novel tigecycline resistance gene, tet(X6), and its variants in four bacterial species isolated from chickens and pigs in China.

Methods: WGS was conducted to identify the suspected resistance genes in the tigecycline-resistant Myroides phaeus 18QD1AZ29W. Functional cloning, homology modelling and molecular docking were performed to compare the function with other Tet(X) variants.