Multi-component strategy for the preparation of covalent organic frameworks-based stationary phase for high resolution separation of isomers.

Functionalization of covalent organic frameworks (COFs) is a critical means to prepare COF-based gas chromatography stationary phases for high-resolution separation. Here, we report a simple and controllable multi-component strategy for the functionalization of COF stationary phases. As a proof of concept, a COF fabricated from 1,3,5-tris(4-aminophenyl) benzene (Tab) and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde (DHTA), Tab-DHTA, was chosen as a model.

Innovating cell culture process development with deep learning-powered robotic experimentation using the first Industrial Smart Lab Framework.

Traditional biologics process development, including antibody and recombinant protein production, typically relies on labor-intensive, iterative cell culture optimization to determine optimal process parameters. To address this inefficiency, we introduced the Industrial Smart Lab Framework for Cell Culture (ISLFCC), an autonomous laboratory that combines deep learning and robotic experimentation to enhance cell culture processes. In this system, robotic arms sample various bioreactors for analysis, and the IoT system transmits these analysis results to decoder-only transformer deep learning models.

Efficient hydrogenation of ketones and aldehydes catalysed by a well-defined PNNP-rhenium complex.

Well-defined rhenium complexes bearing a tetradentate PNNP ligand were developed for the first time. These new complexes exhibit much better performance in catalytic hydrogenation of carbonyl derivatives into alcohols compared to the corresponding aminophosphine-ligand-constituted complex (-PPhCHCHNH)Re(CO)Cl. Under the optimized conditions, the highest turnover frequency and turnover number can be up to 4041 h and 61008, respectively.

Intravitreal injection of cell membrane-coated nanoparticles for retinoblastoma treatment.

Chemotherapy is a widely adopted treatment for malignant intraocular tumors such as retinoblastoma. However, achieving high delivery efficiency remains challenging due to rapid clearance and lack of targeting specificity. Cancer cell membrane-coated nanoparticles have been extensively reported to exhibit specific affinity to homotypic cancer cells.

Automated Lightweight Model for Asthma Detection Using Respiratory and Cough Sound Signals.

Chronic respiratory diseases, such as asthma and COPD, pose significant challenges to human health and global healthcare systems. This pioneering study utilises AI analysis and modelling of cough and respiratory sound signals to classify and differentiate between asthma, COPD, and healthy subjects. The aim is to develop an AI-based diagnostic system capable of accurately distinguishing these conditions, thereby enhancing early detection and clinical management.

Boronate affinity molecularly imprinted covalent organic framework for highly selective recognition and extraction of zearalenone.

Selective and sensitive determination of zearalenone (ZEN) in food matrices is crucial for risk assessment and mycotoxin control due to its high toxicity. Herein, we introduce a boron affinity molecularly imprinted covalent organic framework (BA-MICOF) for selective extraction of ZEN from cereal. The Povarov reaction was employed for one-pot synthesis of boric acid incorporated COF (BA-COF) to avoid complex post-modification.

External stimuli-responsive drug delivery to the posterior segment of the eye.

Posterior segment eye diseases represent the leading causes of vision impairment and blindness globally. Current therapies still have notable drawbacks, including the need for frequent invasive injections and the associated risks of severe ocular complications. Recently, the utility of external stimuli, such as light, ultrasound, magnetic field, and electric field, has been noted as a promising strategy to enhance drug delivery to the posterior segment of the eye.

Unlocking Enhanced Detection of Perfluoroalkanesulfonic Acids via Fluorinated Nonpolar 3D Covalent Organic Frameworks-Based Ambient Probe Nanoelectrospray Ionization Mass Spectrometry.

The trace levels and severe matrix interferences greatly limited the determination of stable, persistent, and long-range-transported perfluoroalkanesulfonic acids (PFSAs) in complex environments. Here, we design and prepare the first fluorinated nonpolar 3D COF (TFPM-Pa-CF) as an adsorbent, consisting of tetrakis(4-formylphenyl)methane (TFPM) and 2,5-diaminobenzo-trifluoride (Pa-CF) for adsorption and extraction of PFSAs. The proposed TFPM-Pa-CF demonstrates excellent adsorption capacity (509.

Kinetic Method Coupled with Thermal-Assisted Paper Spray Ionization Mass Spectrometry for Direct Determination of Enantiomeric Excess of Multiple d/l-Amino Acids in Functional Foods.

Amino acids are commonly used as nutritional fortification substances in functional foods, and their chiral configuration is an important determinant of food function. Rapid chiral screening methods are urgently needed in food analysis but are limited by the long-time chiral separation and matrix interference. In this study, we show a kinetic method coupled to thermal-assisted paper spray ionization mass spectrometry for direct determination of enantiomeric excess () of multiple d/l-amino acids in complex food matrixes without sample pretreatment.

Designing flexible aliphatic linker based molecular imprinted covalent organic framework for rapid and selective extraction and detection of trace zearalenone.

Food and environment contaminations by zearalenone (ZEN) has aroused serious issues in public health. Sensitive and accurate detection of ZEN remains a daunting challenge due to complex interference and low residue in real samples. Herein, we report a novel molecular imprinted covalent organic framework with flexible aliphatic linkers (ALCOF-MIP) for rapid and selective extraction of ZEN in real samples.

Evaluation of the Potential of a Ratiometric Fluorescence Sensor for Sensitively Recognizing 1-Hydroxypyrene in Real Urine Samples Based on a Stable Zn-MOF.

In the work, a 3D Zn-based MOF, [CH(NH)][Zn(obdb)(OH)]·DMF (compound ) has been formed successfully through the self-assembly of Zn(II) ion and organic linker Hobdb (4',4‴-oxybis[1,1'-biphenyl]-3,5-dicarboxylic acid, a high-symmetrical tetra-carboxylate ligand) under solvothermal conditions. In the structure of compound , Zn and the symmetry-generated ones constitute a tetra-nuclear cluster [Zn(μ-COO)(μ-OH)], and then by the coordination of tetra-nuclear clusters and the organic linkers, a 3D stair-like framework containing a 1D rectangular channel with the porosity of 47.3% is generated.

Opto-Epigenetic Regulation of Histone Arginine Asymmetric Dimethylation via Type I Protein Arginine Methyltransferase Inhibition.

Histone arginine asymmetric dimethylation, which is mainly catalyzed by type I protein arginine methyltransferases (PRMTs), is involved in broad biological and pathological processes. Recently, several type I PRMT inhibitors, such as MS023, have been developed to reverse the histone arginine dimethylation status in tumor cells, but extensive inhibition of type I PRMTs may cause side effects in normal tissues. Herein, we designed a photoactivatable MS023 prodrug (C-MS023) to achieve spatiotemporal inhibition of histone arginine asymmetric dimethylation.

Ambient-pressure selective hydrogenation of unsaturated aldehydes and ketones into unsaturated alcohols in the water phase.

A universal and green catalytic system for the hydrogenation of unsaturated aldehydes and ketones into the corresponding alcohols with the CC bonds retained under atmospheric hydrogen pressure in the water phase was realized by -functionalized amino ligand-stabilized ruthenium complexes (-PPhCHNHMe)[(CHNHR)]RuCl (R = H, Me, Et) and (-PPhCHNMe)[(CHNHEt)]RuCl with wide substrate compatibility and excellent functionality tolerance. The structural synergism between -PPhCHNHMe and (CHNHEt) achieves the enhanced performance, with a positive correlation with the electron density of the amino ligand.

Treatment for peripheral nerve injury: a protocol for a systematic review and Bayesian network meta-analysis.

Introduction: Available therapies for peripheral nerve injury (PNI) include surgical and non-surgical treatments. Surgical treatment includes neurorrhaphy, grafting (allografts and autografts) and tissue-engineered grafting (artificial nerve guide conduits), while non-surgical treatment methods include electrical stimulation, magnetic stimulation, laser phototherapy and administration of nerve growth factors. However, the treatments currently available to best manage the different PNI manifestations remain undetermined.

Effects of Visualized Health Education Based on Health Belief Model on Self-Management of Patients Undergoing Maintenance Hemodialysis.

Background: Patients with end-stage renal disease often require maintenance hemodialysis (MHD). Effective self-management is crucial for the prognosis of patients undergoing MHD; however, their self-management practices remain suboptimal.

Objective: This study aimed to explore the effects of visualized health education based on health belief model on self-management, self-efficacy, quality of life, and quality of dialysis in patients undergoing MHD.

Tumor Site-Specific In Vivo Theranostics Enabled by Microenvironment-Dependent Chemical Transformation and Self-Amplifying Effect.

Precise tumor diagnosis and treatment remain complex challenges. While numerous fluorescent probes have been developed for tumor-specific imaging and therapy, few exhibit effective function in vivo. Herein, a probe called TQ-H is designed that can realize robust theranostic effects both in vitro and in vivo.

Phosphorylated Covalent Organic Framework Membranes Toward Ultrafast Single Lithium-Ion Transport.

Developing all-solid-state electrolytes, the chips for lithium-metal batteries, with superior electrochemical and mechanical properties awaits the disruptive materials. Herein, ionic covalent organic framework membranes are explored as solid-state electrolytes for single Li conduction. In the membrane, the anion groups act as Li transporter, determining Li binding capacity and releasing ability, whereas the oxygen-containing groups act as Li co-transporter, creating relay sites between adjacent Li transporters for rapid hopping.

Heterogeneous Covalent Organic Framework Membranes Mediated by Polycations for Efficient Ions Separation.

Precise ions sieving at angstrom-scale is gaining tremendous attention thanks to its significant impact at the water-energy nexus. Herein, a novel polycation-modulated interfacial polymerization (IP) strategy is developed to prepare a heterogeneously charged covalent organic frameworks (COFs) membrane. Cationic poly(diallyldimethylammonium chloride) (PDDA) regulates the growth and assembly of anionic COFs nanosheets, which thus provides a negative, smooth top surface and positive, rough bottom surface, indicating the presence of heterogeneously charged angstrom-scale channels through the membrane.

Reactive Oxygen Species Responsive Supramolecular Prodrug Eyedrops for the Treatment of Choroidal Neovascularization.

Choroidal neovascularization (CNV) represents a hallmark of neovascular fundus diseases, including age-related macular degeneration and diabetic retinopathy. Traditional eyedrops have encountered formidable challenges in treating CNV, primarily due to their extremely poor intraocular bioavailability and potential adverse off-target effects. Herein, an ocular-permeable supramolecular prodrug eyedrop (Di-DAS/P-PCD) has been developed for the on-demand delivery of antiangiogenic agents in the oxidative microenvironment of CNV.

Calixarene incorporated molecular imprinting on covalent organic framework for supramolecular recognition and specific extraction of citrinin.

Specific recognition and selective extraction of mycotoxin in environmental and food matrixes is significant to guarantee public health. Covalent organic frameworks (COFs) are promising adsorbents with tailorable functionality, but their low binding affinity and poor selectivity hamper their wide application for selective extraction of trace mycotoxin from complex matrix. Herein, we report calixarene incorporated molecular imprinting on COF to prepare molecularly imprinted calix[4]arene-containing COF (MICOF-CX4) for supramolecular recognition and specific adsorption of citrinin.

Aptamer-Conjugated Covalent-Organic Framework Nanochannels for Selective and Sensitive Detection of Aflatoxin B1.

Sensitive and selective detection of trace aflatoxin B1 (AFB1) in foods is of great importance to guarantee food safety and quality but still challenging because of its trace amount and the interference from the complex food matrix. Here, we report the integration of aptamer (Apt) and an ordered 2D covalent organic framework (COF) to solid-state anodic aluminum oxide (AAO) nanochannels (Apt/COF/AAO) for selective and sensitive detection of trace AFB1. The high specificity of Apt for AFB1 led to a selective change in the surface charge of Apt/COF/AAO and in turn the current change of the nanochannel, permitting the selective and sensitive determination of trace AFB1 in complex food samples.

Rational fusion design inspired by cell-penetrating peptide: SS31/S-14 G Humanin hybrid peptide with amplified multimodal efficacy and bio-permeability for the treatment of Alzheimer's disease.

Alzheimer's disease is a neurodegenerative disease induced by multiple interconnected mechanisms. Peptide drug candidates with multi-modal efficacy generated from fusion strategy are suitable for addressing multi-facet pathology. However, clinical translation of peptide drugs is greatly hampered by their low permeability into brain.

Light-Activated Anti-Vascular Combination Therapy against Choroidal Neovascularization.

Choroidal neovascularization (CNV) underlies the crux of many angiogenic eye disorders. Although medications that target vascular endothelial growth factor (VEGF) are approved for treating CNV, their effectiveness in destroying new blood vessels is limited, and invasive intravitreal administration is required. Additionally, other drugs that destroy established neovessels, such as combretastatin A-4, may have systemic side effects that limit their therapeutic benefits.

Multivariate analysis of factors associated with the successful prediction of initial blind placement of a nasointestinal tube in the stomach based on X-ray imaging: a retrospective, single-center study.

Background: Patients in the intensive care unit (ICU) are highly susceptible to malnutrition, and while enteral nutrition via nasogastric tube is the preferred method, there is a risk of inadvertent reflux and aspiration. Therefore, clinicians have turned to nasointestinal tubes (NET) for enteral nutrition as an alternative option. But the precise localization of NET presents an ongoing challenge.