A rare cause of diffuse alveolar hemorrhage in a pediatric patient: thigh localization of INI1-deficient epithelioid sarcoma.

Background: Diffuse alveolar hemorrhage (DAH) is a group of rare but life-threatening conditions characterized by bleeding into the alveolar spaces, often associated with various etiologies. Epithelioid sarcoma (ES) is a rare and aggressive soft tissue sarcoma that has never been reported to be associated with DAH.

Methods: We report a rare case of ES in a pediatric patient presenting with DAH and intracranial hemorrhagic lesions.

Impact of climate change on the distribution of Ligustrum leucanthum using MaxEnt modeling.

Anthropogenic climate change has hastened habitat loss and fragmentation for wild animals and vegetation. However, the absence of sufficient and trustworthy information on their geographical distribution has impeded effective conservation management strategies. Ligustrum leucanthum is a drought-resistant tree species that can thrive in heavy metal-contaminated soil.

Quinoxaline-based double responsive fluorescent probe: An effective tool for detection of aniline and p-nitroaniline in the environment.

Aniline and p-nitroaniline, as crucial intermediates in organic synthesis, find extensive applications in the fields of medicine, pesticides, and beyond. However, due to their significant toxicity and carcinogenic potential, these compounds pose severe risks to both ecological environments and human health. In this work, a novel fluorescent probe PAMD for the detection of aniline and p-nitroaniline was constructed and synthesized from camphorquinone.

Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury.

Background: Drug-induced liver injury (DILI) is a major clinical concern due to its unpredictable nature and lack of effective therapeutic options.

Methods: This study investigated the hepatoprotective effects of Atractylodes macrocephala polysaccharides (AMPs) in a mouse model of acetaminophen (APAP)-induced liver injury. Mice were pretreated with AMPs for 7 days prior to APAP challenge, and liver injury was evaluated through histopathology, serum biochemistry, molecular assays, and gut microbiota analysis.

Membrane Fusion Drives Facile Uptake of Cell Membrane-Coated Nanocarriers.

Endocytosis has been the bane of the intracellular delivery efficiency of nanoscale systems. A class of delivery systems, viz., cell membrane-coated nanoparticles (CMNPs), show efficacious and even cell-selective uptake.

Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential.

Although prior studies have identified cumulus cells (CCs)-expressed genes and miRNAs that regulate cumulus expansion and/or CC apoptosis and may serve as markers for selecting competent oocytes and embryos, there remains an urgent need to identify CCs-expressed genes and miRNAs whose expression levels are directly correlated with oocyte developmental potential (DP). In this study, we first established CC models from mouse cumulus-oocyte complexes (COCs) that exhibited significantly different DP following in vitro or in vivo maturation. Subsequently, we performed mRNA/miRNA sequencing and functional analyses using these in vitro and in vivo CC models.

Surface-Induced Conformational Changes of α-Synuclein on Silica Nanoparticles of Varying Sizes Corresponding to Protein Structural Domains: Insights from Enhanced Sampling MD Simulations.

Protein-nanoparticle interactions are crucial in a diverse array of biotechnology and biomedical applications. Variations in nanoparticle sizes can adjust surface interactions with proteins and biomolecules, thereby influencing their conformation and functionality. To achieve precise control over the nanoparticle sizes corresponding to the dimensions of protein structural domains (∼nm) and establish the relationship between nanoparticle curvature and protein conformational changes, we conduct well-tempered metadynamics simulations to explore the secondary structure changes and thermodynamic characteristics of α-synuclein (αS), an intrinsically disordered protein (IDP), adsorbed onto silicon dioxide (SiO) nanoparticles of varying sizes (diameter, = 0.

Myelin oligodendrocyte glycoprotein antibody-associated meningoencephalitis without cerebral parenchyma involvement on MRI: A single-centre paediatric cohort study.

Since meningoencephalitis with prominent meningeal involvement was first described as a rare phenotype of myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD), sporadic cases have been reported. However, most of the reports were case reports and small sample cohorts. We conducted a retrospective analysis of paediatric patients with MOG antibody-associated meningoencephalitis without cerebral parenchyma involvement on imaging at Beijing Children's Hospital between 2017 and 2024.

A novel easily available near-infrared fluorescent probe for rapid and specific detection of Au(Ⅲ) ion in environmental water samples and its biological imaging applications in living cells and zebrafish.

The in-situ tracking of Au contaminant in the environment andorganisms has aroused considerable research interest. Herein, a simple colorimetric and near-infrared probe DPHB (2-3-(4-(dimethylamino)phenyl)allylidene)-6-hydroxybenzofuran-3(2H)-one) was strategically designed and readily synthesized for Au monitoring. DPHB presented a conspicuous fluorescence quenching response toward Au in nearly 100 % aqueous solution.

When Adversarial Training Meets Prompt Tuning: Adversarial Dual Prompt Tuning for Unsupervised Domain Adaptation.

Unsupervised domain adaptation (UDA) aims to adapt models learned from a well-annotated source domain to a target domain, where only unlabeled samples are available. To this end, adversarial training is widely used in conventional UDA methods to reduce the discrepancy between source and target domains. Recently, prompt tuning has emerged as an efficient way to adapt large pre-trained vision-language models like CLIP to a variety of downstream tasks.

Synergistic Effects of Polycationic and Polyfluorinated Functionalities for Efficient Intracellular Protein Delivery.

Intracellular protein therapy is a promising strategy in biologics, including vaccine development, gene editing, and cancer therapeutics. However, protein-based drug delivery remains a significant challenge, particularly in penetrating cell barriers to reach intracellular targets. Inspired by transport adjuvants, we designed a series of polymeric vectors to achieve efficient functional protein trafficking with low cytotoxicity.

A Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detecting Hydrogen Sulfide in Environmental Waters, Wine Samples, and Living Systems.

Hydrogen sulfide (HS) is closely related to physiological diseases, environmental pollution, food safety, and plant growth. Herein, a new nopinone-based near-infrared fluorescent probe has been developed to detect HS with superior performance, including a large Stokes shift, low detection limit, good selectivity, and fast response time. was used to detect HS in environmental water and wine samples quantitatively.

Construction of a flavonol-based colorimetric and fluorescent probe with large Stokes shift for detecting hypochlorous acid in foodstuffs and imaging in living cells, zebrafish and plants tissues.

The excessive addition of hypochlorous acid (HClO) during food preservation can reduce the nutritional value of foodstuffs, and its abnormal levels in organisms will affect normal physiological and pathological activities. Herein, an efficient fluorescent probe HTTPC for monitoring HClO in foodstuffs and biosystems has been developed from natural flavonol. HTTPC itself emitted intense orange fluorescence emission in nearly 100 % aqueous solution, and it underwent a remarkable fluorescence quenching effect after being treated with HClO.

Construction of a novel cuproptosis-related gene signature and integrative analyses in cholangiocarcinoma.

Background: Cuproptosis is a unique form of cell death that is dependent on copper, which is fundamentally different from other recognized forms of cell death. However, the molecular and immune characteristics in cuproptosis-defined subgroups of cholangiocarcinoma (CCA) remain to be further illustrated.

Methods: We conducted a comprehensive investigation into the genetic and transcriptional variation, prognostic significance, and expression profiles of 16 cuproptosis-related genes (CRGs).

Cumulus cells and the TNF-alpha signaling facilitate aging of ovine oocytes.

Post-maturation oocyte aging (PMOA) is known to significantly impair the developmental potential of oocytes; however, comprehensive studies on ovine PMOA remain limited. In mice, cumulus cells (CCs) accelerate oocyte aging by releasing cytokines, but the roles of CCs and cytokines in PMOA of domestic animals are poorly understood. This study aimed to elucidate the involvement of CCs and tumor necrosis factor (TNF)-α in the PMOA of ovine oocytes.

Safety and Efficacy of Anlotinib-based Regimen in Patients with Unresectable or Metastatic Bone and Soft-tissue Sarcomas: A Retrospective Institutional Study.

Background: Anlotinib has demonstrated durable clinical benefits in patients with unresectable or metastatic bone and soft-tissue sarcomas.

Methods: 92 patients treated with chemotherapy combined with or without anlotinib were collected and analyzed. The objective response rate (ORR) and disease control rate (DCR) were analyzed.

A novel carbazole-pyrimidine-based dual mode fluorescent probe for detection of acidic and basic pH in biological systems.

pH balance is an important factor in regulating the internal environment of body and maintaining the normal physiological activities, but pH cannot be detected in vivo without damaging the tissue. It is important to develop a pH probe with low toxicity, high sensitivity and targeting of organelles. In this research, a novel carbazole-pyrimidine-based probe PKZP was designed from 2-hydroxyl-3-pinanone which was derived from natural monoterpene α-pinene for detecting both acidic and basic pH in vivo.

A novel BODIPY-derived colorimetric and ratiometric dual-mode fluorescent probe for highly sensitive and visual detection of sulfite in food and living organisms.

Sulfite, widely used as a food additive, performs indispensable functions in the field of food sterilization, bleaching, and antisepsis. However, the overuse of sulfite may destroy food nutrition and pose health risks to people. In this work, an innovative BODIPY-based fluorescent probe (BODIPY-DBC) was constructed for highly sensitive recognition of sulfite.

Inhibition of α-Synuclein Misfolding into β-Sheet Domains on Medium-Sized Gold Nanoclusters: Evidence from Enhanced Sampling MD Simulations.

Targeting Parkinson's disease (PD) related protein, α-synuclein (αS), via gold nanoclusters (AuNCs) has received considerable attention in PD treatments, but its molecular basis on the initial interactions between αS and AuNCs remains elusive due to the absence of a unique secondary structure of αS chains. Here, at the single-cluster level, we incorporate well-tempered metadynamics simulations to explore the structural and thermodynamic characteristics of the full length αS adsorbed on different-sized AuNCs (Au, = 25, 36, 44, 68, 102) with modeled thiolated ligands (Au@Lig). The conformational landscapes of αS indicate that uncharged Au@SCHOH chaperones the native intrinsically disordered conformations of αS, while negatively and positively charged AuNCs greatly increase the likelihood of forming intramolecular β-sheet domains, which are necessary for αS fibrillation and are a hallmark of PD.

Synthesis of a new camphor-derived carboxylesterase-activated fluorescent probe for sensitive detection of dimethoate residues in agricultural products and its applications in biological systems.

In this paper, a camphor-derived enzyme activatable probe CPA was synthesized for detecting dimethoate pesticide. The ester bond of probe CPA could be selectively hydrolyzed and fragmented in the presence of carboxylesterase (CE), which caused a remarkably enhanced green fluorescence signal at 501 nm. Probe CPA could function as an effective fluorescent platform for the sequential detection of dimethoate due to the obvious inhibition effect of dimethoate on the activity of CE.

A novel fluorescent probe for fast detection of sulfur dioxide derivatives in water, soil, food samples and its applications in biological imaging.

Sulfur dioxide (SO) has a wide range of applications in food additives and industrial production, and it is one of the main substances that form acid rain, causing serious harm to ecosystems and human health. Hence, it is necessary to construct an effective tool to quickly and accurately detect SO derivatives in environmental, food, and biological samples. In this study, fluorescent probe NPMQ was built to detect SO derivatives from nopinone with the merits of superior water solubility, high sensitivity (12 nM), excellent specificity, large Stokes shift (180 nm), and rapid response time (within 5 s).

Seasonal dynamics of seed dormancy and germination in the weed .

Background: Understanding the reproductive biology of weeds is crucial for managing them effectively. (Poaceae) is a widely distributed weed species that poses significant challenges to agricultural productivity. Nevertheless, it remains unclear how the soil seed bank of responds to environmental shifts, and whether a dormancy cycle is present in this species.

Antibody-Directing Antibody Conjugates (ADACs) Enabled by Orthogonal Click Chemistry for Targeted Intracellular Delivery.

Using orthogonal click chemistries for efficient nanoscale self-assembly, a new antibody-directing antibody conjugate (ADAC) nanogel is generated. In this system, one of the antibodies is displayed on the nanogel surface to specifically recognize cell-surface epitopes while the other antibody is encapsulated inside the nanogel core. The system is programmed to release the latter antibody in its functional form in the cytosolic environment of a specific cell to engage intracellular targets.

A chromosome-level genome assembly provides insights into the local adaptation of Tamarix austromongolica in the Yellow River Basin, China.

Tamarix austromongolica is endemic to the Yellow River Basin and has adapted to diverse ecological settings in the region, including the arid areas of northwestern China and the saline soil regions of the Yellow River Delta. However, the genetic basis of its local adaptation remains unclear. We report a chromosome-level assembly of the T.

Seasonal variations of organophosphate esters (OPEs) in atmospheric deposition, and their contribution to soil loading.

Organophosphate esters (OPEs) are ubiquitous in surface soil, and atmospheric deposition is considered to be the major pollution source. However, the research on the environmental transport behaviors of OPEs between atmospheric deposition and soil is very limited. In this study, we investigated the contamination levels and seasonal variations of OPEs in atmospheric deposition samples (n = 33) collected from an area of South China every month between February 2021 and January 2022, and evaluated the contribution of OPEs in atmospheric deposition to soil.