Application of punch excision in the treatment of severe burn scars.

Introduction: This study aims to identify the therapeutic outcomes of punch excision in severe burn patients with extensive, excessive and long-lasting growth scars.

Methods: A retrospective comparative clinical observational study was conducted on patients with burn hypertrophic scars who were treated at the Department of Burn and Skin Wound Repair, First Hospital of Jilin University, between October 2022 and October 2024.

Results: A total of 49 patients with severe burn scars were included.

Herpes simplex virus-thymidine kinase/ganciclovir suppressed the growth of lung adenocarcinoma cells accompanied by premature senescence.

Background: Extensive laboratory research and clinical trial results have shown that herpes simplex virus-thymidine kinase/ganciclovir (HSV-TK/GCV) system has a therapeutic effect on various tumours. This study focused on the role of HSV-TK/GCV system therapy for lung adenocarcinoma.

Methods: Cell proliferation, migration, invasion, and premature senescence were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay, wound-healing assay, invasion assay, and β-galactosidase staining, respectively.

The YTHDC1 reader protein recognizes and regulates the lncRNA MEG3 following its METTL3-mediated mA methylation: a novel mechanism early during radiation-induced liver injury.

While apoptotic cell death is known to be central to the pathogenesis of radiation-induced liver injury (RILI), the mechanistic basis for this apoptotic activity remains poorly understood. N-methyladenosine (mA) modifications are the most common form of reversible methylation observed on lncRNAs in eukaryotic cells, with their presence leading to pronounced changes in the activity of a range of biological processes. The degree to which mA modification plays a role in the induction of apoptotic cell death in response to ionizing radiation (IR) in the context of RILI remains to be established.

Substituent effect on the ligand-centred electrocatalytic hydrogen evolution of antimony(III) corroles.

A series of antimony(III) triaryl corroles bearing different numbers of -pentafluorophenyl substituents was synthesized and evaluated as catalysts for electrocatalytic hydrogen evolution. Introduction of the electron-withdrawing pentafluorophenyl group to the corrole macrocycle led to a significant boost in electro-catalytic hydrogen evolution reaction (HER) activity in organic and aqueous media, highlighting the tunability of these complexes for enhanced catalytic activity.

Combination therapy of pulsed dye laser and ablative fractional carbon dioxide laser for the treatment of pediatric postburn scar: a systematic review.

This study aims to determine the effectiveness of combining pulsed dye laser (PDL) and ablative fractional carbon dioxide laser (AFCL) in the treatment of scars in pediatric patients, as well as to identify appropriate treatment parameters, initial treatment timing, and treatment intervals through a systematic literature review. A literature search was conducted between April 6, 2014 and April 6, 2024 in four databases to select studies on the effects of the combination of carbon dioxide fractional laser and pulsed dye laser therapy in children hypertrophic burn scars. This systematic literature review included 497 participants across 8 studies, with individual study sample sizes ranging from 17 to 125 participants.

Evaluation of the lncRNA-miRNA-mRNA ceRNA network in lungs of miR-147 mice.

Previous studies have documented important roles for microRNA-147 (miR-147) in inflammation, radiation-induced injury, cancer, and a range of other diseases. Murine lungs exhibit high levels of miRNA, mRNA, and lncRNA expression. However, very little research to date has focused on the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) networks associated with miR-147, and the regulation of lncRNAs and miRNAs in this setting remains poorly understood.

Clinical phenotype and genetic function analysis of a rare family with hereditary leiomyomatosis and renal cell carcinoma complicated with Birt-Hogg-Dubé syndrome.

To date, over 200 families with hereditary leiomyomatosis and renal cell carcinoma (HLRCC) and over 600 families with Birt-Hogg-Dubé (BHD) syndrome have been reported, with low incidence. Here, we describe a patient with suspected rare HLRCC complicated by BHD syndrome. The proband (II1) had characteristic cutaneous leiomyoma-like protrusions on the neck and back, a left renal mass and multiple right renal, liver and bilateral lung cysts.

PARP-1: a critical regulator in radioprotection and radiotherapy-mechanisms, challenges, and therapeutic opportunities.

Since its discovery, poly (ADP-ribose) polymerase 1 (PARP-1) has been extensively studied due to its regulatory role in numerous biologically crucial pathways. PARP inhibitors have opened new therapeutic avenues for cancer patients and have gained approval as standalone treatments for certain types of cancer. With continued advancements in the research of PARP inhibitors, we can fully realize their potential as therapeutic targets for various diseases.

Clinical and imaging features of six Han patients with SAPHO syndrome.

Background: Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome is a rare autoimmune disease characterized by skin or osteoarticular damage. SAPHO syndrome is often misdiagnosed or missed diagnosis due to lack of overall understanding of the disease by clinicians.

Purpose: To analyze the clinical symptoms and imaging features of six Han patients with SAPHO syndrome in order to provide reference for doctors to diagnose SAPHO syndrome.

Case report and analysis: Behçet's disease with lower extremity vein thrombosis and pseudoaneurysm.

Background: Behçet's disease (BD) is a unique autoimmune chronic systemic vasculitis that affects veins and arteries of all sizes. BD can lead to recurrent vascular events, especially venous thrombosis, with an incidence rate of 40%, or pseudoaneurysms formed under long-term inflammatory reaction or iatrogenic stimulation. BD-related risk factors promote endothelial dysfunction, platelet activation and overactivation of tissue factors leading to mural inflammatory thrombi.

Association of menopausal status with COVID-19 outcomes: a propensity score matching analysis.

Background: Despite the growing number of studies on the coronavirus disease-19 (COVID-19), little is known about the association of menopausal status with COVID-19 outcomes.

Materials And Methods: In this retrospective study, we included 336 COVID-19 inpatients between February 15, 2020 and April 30, 2020 at the Taikang Tongji Hospital (Wuhan), China. Electronic medical records including patient demographics, laboratory results, and chest computed tomography (CT) images were reviewed.

Genetic diagnosis history and osteoarticular phenotype of a non-transfusion secondary hemochromatosis.

Background: It is not easy to identify the cause of various iron overload diseases because the phenotypes overlap. Therefore, it is important to perform genetic testing to determine the genetic background of patients.

Aim: To investigate the genetic background of a patient with hemochromatosis complicated by psoriasis on both lower extremities.

Complicated Posterior Cervical Skin and Soft Tissue Infections at a Single Referral Center.

Background: Although the incidence and mortality of complicated skin and soft tissue infections have decreased, this infection is still relatively frequent and can be associated with lethal complications. In this study, the authors present our clinical experience of patients with complicated posterior cervical skin and soft tissue infections (CPCSSTIs) diagnosed and treated in a reconstructive unit in northeastern China.

Methods: A retrospective chart review of patients diagnosed with CPCSSTIs from January 2009 to December 2018 was performed.

Lower energy radial shock wave therapy improves characteristics of hypertrophic scar in a rabbit ear model.

The aim of the present study was to investigate the effects of radial extracorporeal shock wave therapy (rESWT) on scar characteristics and transforming growth factor (TGF)-β1/Smad signaling in order to explore a potential modality for the treatment of hypertrophic scars (HS). The HS model was generated in rabbit ears, then rabbits were randomly divided into 3 groups: Lower (L)-ESWT [treated with rESWT with lower energy flux density (EFD) of 0.1 mJ/mm], higher (H)-ESWT (treated with a higher EFD of 0.

Necrotizing soft tissue infection: clinical characteristics and outcomes at a reconstructive center in Jilin Province.

Background: To investigate the clinical characteristics and treatment outcomes in necrotizing fasciitis (NF) patients in a reconstructive unit in northeastern China.

Methods: Medical records of patients diagnosed with and treated for NF in the extremities from November 2013 to December 2016 were retrospectively reviewed. Demographic data, clinical presentation, duration of signs and symptoms, location of infection, predisposing factors, causative microbiological organisms, laboratory risk indicator for necrotizing fasciitis (LRINEC) score, number of surgical debridements, length of hospital stay, treatments, and outcomes were recorded.

Couple-kissing flaps for successful repair of severe sacral pressure ulcers in frail elderly patients.

Background: Surgical repair of severe pressure ulcers (PUs) in elderly patients remains a challenge for clinicians due to the complicated comorbidities and the special physical characteristics of elderly patients. The objective of this study was to evaluate the application of couple-kissing flaps (CKF) in the reconstruction of sacral PUs in these patients.

Methods: Elderly patients (over 70 years) with stage 3 or stage 4 PUs who underwent CKF immediately after radical debridement between July 2012 and December 2015 were enrolled in this retrospective study.

Transcriptome analysis of the mammary gland from GH transgenic goats during involution.

Mammary glands are organs for milk production in female mammals. Growth hormone (GH) is known to affect the growth and development of the mammary gland, as well as to increase milk production in dairy goats. This study performed a comprehensive expression profiling of genes expressed in the mammary gland of early involution GH transgenic (n=4) and non-transgenic goats (n=4) by RNA sequencing.

Enhancement of transfection efficiency with NLS and SPB-NLS.

Low positive cell screening efficiency severely hinders the development of transgenic animals. The major rate-limiting step of positive cell screening is DNA entering the nucleus, particularly for large DNA molecules. To enhance the transport of large DNA molecules into the nucleus, particularly for the production of transgenic animals, nuclear localization sequence (NLS) peptides and the peptide derivative succinimidyl-[4-(psoralen-8-yloxy)]-butyrate (SPB)-NLS were synthesized to mediate transfection in vitro.

Enhancement of gene transfer efficiency in the Bcap-37 cell line by dimethyl sulphoxide and menthol.

Simple and efficient gene transfer into the nucleus would facilitate non-viral gene delivery. One promising method of non-viral gene delivery is to apply penetration enhancers. Chemicals, such as dimethyl sulfoxide (DMSO) and menthol, may have promise as non-toxic vehicles in improving gene transfer efficiency.

[Design and experiment of micro biochemical detector based on micro spectrometer].

According to the requirements of rapid detection of important life parameters for the sick and wounded, a new micro bio-chemical detection configuration was proposed utilizing continuous spectroscopy analysis, which was founded on MOEMS and embedded technology. The configuration was developed as so much research work was carried out on the detecting objects and methods. Important parameters such as stray light, absorbance linearity, absorbance ratability, stability and temperature accuracy of the instrument were tested, which are all in good agreement with the design requirements.

Diversity of tight junctions (TJs) between gastrointestinal epithelial cells and their function in maintaining the mucosal barrier.

The gastrointestinal epithelium, which is covered by a single layer of epithelial cells, including enterocytes, intraepithelial lymphocytes, goblet cells, microfold cells, and dendritic cells, serves as a protective barrier separating luminal contents from the underlying tissue compartments. The epithelium plays an important role in the first line of host defense against a variety of pathogens, as well as maintaining the homeostasis in gastrointestinal tract. All these epithelial cells express junction complex proteins and form cell junctions such as adherens and TJs, although the TJs have small differences among different epithelial cells.

Use of green fluorescent protein to monitor Lactobacillus in the gastro-intestinal tract of chicken.

Lactobacilli, like other gut commensal bacteria, are well known for their use in industrial food fermentations and for their probiotic properties. However, little is known about the interaction of these microorganisms with the gastro-intestinal epithelia when administered in vivo. To specifically monitor the passage of lactobacilli after oral administration, the gfp gene was cloned downstream from the constitutive l-lactate dehydrogenase promoter (pldhL) in the experiment.