Identification of TMED10 as A Regulator for Neuronal Exocytosis of Amyloid Beta 42.

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by neurotoxic amyloid beta (Aβ) deposition in the brain. Neurons can internalize and exocytose Aβ; however, the molecular pathways governing Aβ release remain poorly understood. To identify key regulators of Aβ42 transport, we applied formaldehyde cross-linking of protein complexes combined with co-immunoprecipitation and mass spectrometry analysis to identify TMED10 as a novel Aβ42-interacting protein.

Clinical and genetic features of UNC13D deficiency with hypogammaglobulinemia.

Background: UNC13D deficiency is the most common form of familial hemophagocytic lymphohistiocytosis (FHL) in Asia. Hypogammaglobulinemia is a rare phenotype observed in both patients with FHL3 and sporadic hemophagocytic lymphohistiocytosis (HLH). Our observations suggest that UNC13D deficiency with hypogammaglobulinemia presents a distinct clinical phenotype compared to other HLH patients.

Peptide codes for organ-selective mRNA delivery.

Organ-selective delivery of messenger RNA (mRNA) is critical for fulfilling the therapeutic potential of mRNA-based gene and protein replacement technologies. Despite clinical advances in the hepatic delivery of mRNA using lipid nanoparticles (LNPs), current strategies for extrahepatic-organ-selective mRNA delivery still have limitations. Here we report a peptide-encoded organ-selective targeting (POST) method for the delivery of mRNA to extrahepatic organs after systemic administration, which is based on the modular tuning of LNPs through surface engineering with specific amino acid sequences (POST codes).

[A reporter gene assay for determining antibody-dependent cell-mediated phagocytosis activity of HER2-targeted antibody drug conjugate].

To develop a method for determining the antibody-dependent cell-mediated phagocytosis (ADCP) activity of human epidermal growth factor receptor 2 (HER2)-targeted antibody drug conjugate (ADC) based on the reporter gene assay, we established an ADCP activity assay with Jurkat/NFAT/FcγRIIa cells as the effector cells and BT474 as the target cells. Then, the target cell density, the ratio of effector to target cells, the target cell adhesion time, the incubation time for drug administration, and the induction time after adding effector cells were optimized by the method of design of experiment (DOE). The method showed a significant dose-response relationship, which was complied with the four-parameter equation: =(A-D)/[1+(/C)]+D.

MitoQ alleviates m.3243A>G-induced mitochondrial dysfunction by stabilizing PINK1 and enhancing mitophagy.

The mitochondrial 3243A>G mutation (m.3243A>G) is associated with diverse clinical phenotypes. To elucidate the underlying mechanisms and explore intervention strategies in m.

Leveraging diverse liquid-liquid phase separation patterns to predict the prognosis and immunotherapy of pediatric acute myeloid leukemia.

Background: Despite improvements in 5-year survival rates for pediatric acute myeloid leukemia (P-AML) over recent decades, the relapse rate remains high. This challenge is compounded by the absence of reliable prognostic biomarkers, which limits the effectiveness of predictive, preventive, and personalized approaches in P-AML management. Emerging evidence has indicated that the aberrant liquid-liquid phase separation (LLPS) can alter the spatiotemporal coordination of biomolecular condensates, thereby contributing to tumorigenesis and progression.

Sulfur form regulation and dual-interface effect: catalytic mechanism of nickel-based catalyst in ethanol electrooxidation.

Electrocatalytic organic oxidation emerge as energy-efficient alternatives to conventional oxygen evolution reactions (OER) for sustainable hydrogen coproduction. The design of efficient catalysts and the understanding of the underlying mechanisms of anodic nucleophilic reagent electrooxidation constitute the core of electrochemistry-driven technological advances. Herein, this paper reports a nickel sulfide heterostructure embedded in biomass carbon (NiS-NiS/CC), which exhibits great ethanol oxidation reaction (EOR) activity with a current density of 50 mA·cm at 1.

CD81 senescent-like fibroblasts exaggerate inflammation and activate neutrophils via C3/C3aR1 axis in periodontitis.

Periodontitis, a prevalent inflammatory disease worldwide, poses a significant economic burden on society and the country. Previous research has established a connection between cellular senescence and periodontitis. However, the role and mechanism of cell senescence in the progression of periodontitis have not been thoroughly investigated.

Effect of Clarithromycin Combined With Oxymetazoline Hydrochloride Spray on Olfactory Function and Inflammatory Factors in Patients With Acute Exacerbations of Chronic Rhinosinusitis.

This study is intended to expound the effect of clarithromycin combined with oxymetazoline hydrochloride (OZH) spray in acute exacerbations of chronic rhinosinusitis (AECRS). Patients with AECRS were retrospectively collected and divided into a control group and an observation group. The control group received clarithromycin, and the observation group received OZH spray in addition to clarithromycin.

The Giant Panda Transferrin Receptor Facilitates Feline Parvovirus Infection to Drive Cross-Species Transmission.

Feline parvovirus (FPV) causes feline panleukopenia, a highly contagious disease in cats, marked by severe leukopenia, biphasic fever, diarrhea, vomiting, and hemorrhagic enteritis. Recently, FPV infection in giant pandas has increased, causing diarrhea and ultimately fatal outcomes, thereby threatening their survival and reproduction. Here, we investigated the transmission of FPV in giant pandas and its interaction with cellular receptors using an FPV strain (pFPV-sc) isolated from giant panda feces.

Glycolic Acid Ethoxylate Oleyl Ether Hydrogel Reinforced by 2D Layered Metal Carbide: A Potential Lubricant.

This study investigates the rheological and tribological properties of HO/glycolic acid ethoxylate oleyl ether (HO/Oleth10) and 2D layered metal carbide aqueous/Oleth10 (MXene/Oleth10) hydrogels for lubrication applications. Notably, this work reports the first MXene-reinforced Oleth10 hydrogel, demonstrating how nanomaterial-surfactant synergy enables adaptive lubrication with 200% rheological enhancement and 35% friction reduction. Both hydrogels were characterized as non-Newtonian fluids with grease-like semisolid behavior, governed by hydrogen bonding and weak physical interactions.

Enhanced lignocellulose biomass hydrolysis in a confined space with cellulase-containing liposomes for high-levels succinic acid production.

The production of high-value succinic acid (SA) from waste jatropha fruit shell (JFS) lignocellulose biomass is considered to be an economical and environmentally friendly strategy. Firstly, the cellulase encapsulated in liposomes was used to construct an efficient enzyme-catalyzed microenvironment. Furthermore, the hydrolysis efficiency of JFS was enhanced by simultaneously activating lignocellulose and in-situ saccharification in ionic liquid (IL).

Financial toxicity and quality of life among older adults with cancer: a moderated mediation model of loneliness and social support.

Purpose: Financial toxicity (FT) is prevalent among older adults with cancer due to the expensive costs of cancer treatment and causes a decline in quality of life (QoL). This study aimed to get insights into the potential mechanisms of how FT impacts QoL through a moderated mediation model and explore the mediating role of loneliness and the moderating role of social support.

Methods: A cross-sectional survey from May to October 2021 was conducted to recruit older adults with cancer aged 60 years and above in Shanghai, China.

Serological and molecular survey of hepatitis E virus in pets in shaanxi, China.

Background: Hepatitis E virus (HEV) is a zoonotic pathogen, and its infection is widespread in China. However, few studies were carried out on pets, especially in western China. Therefore, this study aimed to characterize the prevalence of HEV infection in pet dogs, cats, and rabbits.

The effects of single and multiple BOPPPS-integrated model sessions on public health students' competences: a two-stage study combining quasi-experimental and cross-sectional analyses.

Background: Effective instructional approaches for fostering students' competences in public health education remain to be explored. This study aimed to integrate the bridge-in, objective, pre-assessment, participatory learning, post-assessment, and summary (BOPPPS) framework with problem-based learning (PBL), team-based learning (TBL), and case-based learning (CBL) to develop a novel instructional model, termed the BOPPPS-integrated model (BIM), and to assess the influences of single and multiple BIM interventions on the competences of undergraduate public health students.

Methods: A two-stage study was conducted by a quasi-experimental pilot study and a cross-sectional survey.

Evidence-based weight-bearing protocols after meniscal repair: balancing functional recovery and healing safety across injury types.

Weight-bearing activity was the primary rehabilitation problem after knee meniscus repair, a scientific weight-bearing protocol was an important guarantee for injury healing and functional improvement. With the popularization of the concept of enhanced recovery after surgery, accelerated weight-bearing activities after knee meniscus repair surgery had gradually gained clinical promotion. However, the effectiveness and safety of accelerated weight-bearing activities were still controversial, and the appropriate weight-bearing protocol after knee meniscus repair with different injury types was still unclear.

Binding of Chinese quince oligomeric proanthocyanidins with different structures to sarcoplasmic and myofibrillar protein in chicken: Role of conformational changes and heterocyclic amine inhibition.

Proanthocyanidins (PAs) bound to proteins affected the inhibitory effect of heterocyclic amines (HCAs). This study evaluated how different structural PAs (catechin, CAT, and epicatechin gallate, ECG) interacted with sarcoplasmic (SP) and myofibrillar protein (MP), and how PA-protein complexes inhibited HCAs. The binding rate of ECG-protein complexes was higher than CAT-protein complexes.

Hyperoside inhibits PRRSV proliferation via the TLR4/NF-κB and p62-Nrf2-Keap1 signaling pathways, mediating inflammation and autophagy.

Porcine reproductive and respiratory syndrome virus (PRRSV) causes abortion and respiratory disease in swine, hindering the development of the pig farming industry worldwide. However, at present, there are no effective vaccines or drugs for PRRSV control. In this study, we evaluated the inhibitory effect of hyperoside on PRRSV replication and and explored the underlying mechanisms.

Formation of phenolic compounds from roasted sesame hull lignin: Impact of lignin structure.

The phenolic compounds generated from sesame hull lignin roasting contribute to sesame oil quality, yet their formation mechanism remains unclear. In this study, a thermal desorption unit with gas chromatography coupled to mass spectrometry (TDU-GC-MS) was used to simulate the roasting of five lignins isolated from sesame hull: two types of milled wood lignin (MWL-1 and MWL-2), acetic acid lignin (AAL), alkali lignin (AL), and ethanol lignin (EL). The effect of lignin structure on phenolic product formation during roasting was investigated.

Multigenerational toxicity and transcriptomic changes induced by environmentally relevant concentrations of microcystin-LR and -RR in Caenorhabditis elegans.

Microcystin (MC) contamination in surface water is currently receiving considerable attention as a public health concern. Seasonal occurrence MCs in aquatic environment suggests their long-term impacts on ecosystem and human health, whereas multi-generational toxic effects of MCs were poorly investigated. The reproductive system is the second most important target organ of MCs after the liver.

Enzyme treatment combined with Maillard reaction modification of sunflower meal hydrolysates to prepare sunflower seed oil flavor enhancer.

Promoting the Maillard reaction (MR) is an effective way to enhance the flavor of oils. To produce fragrant sunflower oil with low oxidation, a mixture of cold-pressed sunflower oil and enzymatic hydrolysates from sunflower meal was heated to 120 °C. The aroma profiles were compared using SAFE-GC-MS/O-based flavoromics, while free amino acids, reducing sugars, and browning intensity were measured.

Salidroside can protect against ferroptosis in cardiomyocytes and may be related to the regulation of GGT1.

Indroduction: Ferroptosis, an iron-dependent cell death mechanism driven by lipid peroxidation, represents a novel therapeutic target for myocardial injury. Salidroside (SAL), a natural bioactive compound derived from Rhodiola rosea, exhibits cardioprotective effects through multi-target mechanisms with minimal adverse effects, yet its precise role in ferroptosis regulation remains unclear.

Methods: This study systematically investigated SAL's anti-ferroptotic effects using (RSL3-induced H9C2 cardiomyocytes) and (DOX-induced myocardial injury mouse model) approaches.

Evaluating the Immunogenic Potential of ApxI and ApxII from : An Immunoinformatics-Driven Study on mRNA Candidates.

Porcine infectious pleuropneumonia (PCP) caused by (APP) leads to severe economic losses in swine production. Commercial vaccines offer limited cross-protection for the 19 serotypes, while APP mRNA vaccines remain unexplored. This study evaluated eight candidate APP proteins (ApxI-IV, OlmA, TbpB, GalT, and GalU) using immunobioinformatics tools, and their immunogenicity and cross-protection were assessed in a mouse model.

Sleep quality among solid organ transplant recipients during the COVID-19 reopening period in China: the interplay of SARS-CoV-2 infection, mental distress, and physical activity.

Objectives: Given immunosuppression and comorbidities, solid organ transplant (SOT) recipients are more prone to infections during the COVID-19 pandemic. However, little is known about the relationship between SARS-CoV-2 infection and sleep quality in this population. This study aimed to investigate the association between SARS-CoV-2 infection and sleep quality among SOT recipients, examine the moderating roles of anxiety and depression, and explore whether the moderating effects vary with physical activity levels.