A High-Affinity and Potently Neutralized Nanobody against Zika Virus.

ZIKA virus (ZIKV), an emerging pathogen for which no licensed drugs or vaccines exist, has attracted global attention. ZIKV envelope protein domainIII (EDIII) can induce neutralizing antibodies with minor cross-reactive epitopes among flaviviruses. Currently, no nanobodies against the ZIKV EDIII protein are available.

Oxygen-doped carbon-supported palladium nanoparticles boost the tandem hydrogenation-acetalization-hydrogenolysis of phenols and diphenyl ethers to cyclohexyl ethers.

The one-pot hydrotreatment of phenols to cyclohexyl ethers is crucial but difficult to achieve for fine chemical synthesis owing to the easy overhydrogenation to cyclohexanols over traditional metal-acid bifunctional catalysts. Herein, surface oxygen-doped carbon-supported Pd nanoparticles (Pd/C-O) were prepared via nitric acid oxidation and subsequent incipient wetness impregnation, demonstrating the tandem hydrogenation-acetalization-hydrogenolysis route of phenol to cyclohexyl methyl ether, achieving an significant yield of 97.9% in a methanol solvent at a low temperature of 110 °C.

Suppressing MASH fibrotic progression by blocking succinate-GPR91 signaling in HSCs.

Background And Aims: The succinate receptor GPR91 is highly expressed in HSCs, with its expression further elevated during metabolic dysfunction-associated steatohepatitis (MASH)-induced fibrotic progression. However, convincing in vivo data on whether blocking GPR91 signaling leads to fibrotic regression in MASH are lacking.

Approach And Results: MASH models were induced by choline-deficient amino acid-defined diet feeding along with lipopolysaccharide injection (CDAA-LPS) plus i.

An Easy-to-Use Risk Stratification System for NSTE-ACS Patients Combining Autonomic Nervous System and Coronary Physiology.

The evaluation of autonomic nervous system (ANS) function and coronary physiology through quantitative flow ratio (QFR) analysis provides a precise method for assessing the severity and prognosis of acute coronary syndrome (ACS). This study aimed to develop and validate a risk score model for predicting the long-term prognosis of non-ST-elevation ACS (NSTE-ACS) patients who underwent complete and successful percutaneous coronary intervention (PCI). NSTE-ACS patients who underwent complete and successful PCI with preoperative and postoperative QFR measurements between January 2018 and December 2020 in our medical center were included.

Piezo2 expressed in ganglionated plexi: Potential therapeutic target of atrial fibrillation.

Background: Atrial fibrillation (AF) is closely linked to autonomic nervous system activity, with activation of the cardiac ganglionated plexi (GP) often present in AF. Piezo, a mechanosensitive ion channel, plays a pivotal role in neuronal modulation. However, its involvement in the GP and AF remains poorly understood.

Analysis of single-cell and spatial transcriptomics in TNBC cell-cell interactions.

Triple-negative breast cancer (TNBC) is a highly malignant tumor in women, characterized by high morbidity, mortality, and recurrence rates. Although surgical treatment, radiotherapy, and chemotherapy are the mainstays of current treatment methods, the high heterogeneity of TNBC results in unsatisfactory outcomes with low 5-year survival rates. Rapid advancements in omics technology have propelled the understanding of TNBC molecular biology.

Selective Hydrogenolysis of Biomass-Derived Aromatic Alcohols over 2D-MoCO MXene by a Reversible Redox-Relay Mechanism.

Selective CH-OH hydrogenolysis of biomass-derived aromatic alcohols to produce methyl aromatics is crucial for synthesizing sustainable fuels and chemicals; yet traditional metal-acid bifunctional catalysis possesses significant challenges owing to its complex reaction network. Herein, a 2D-MoCO MXene was fabricated, demonstrating an efficient hydrogenolysis of 5-hydroxymethyl furfural into 5-methyl furfural, achieving an impressive yield (99.5 %) at the mild reaction temperature of 90 °C.

Homeobox protein MSX-1 restricts hepatitis B virus by promoting ubiquitin-independent proteasomal degradation of HBx protein.

Hepatitis B virus (HBV) X protein (HBx) is a key factor for regulating viral transcription and replication. We recently characterized homeobox protein MSX-1 (MSX1) as a host restriction factor that inhibits HBV gene expression and genome replication by directly binding to HBV enhancer II/core promoter (EnII/Cp) and suppressing its promoter and enhancer activities. Notably, HBx expression was observed to be repressed more drastically by MSX1 compared to other viral antigens.

Integrating the GRACE Score with the Ceramide Risk Score Enhances the Predictive Accuracy of Major Adverse Cardiac Events in Patients with Acute Coronary Syndrome Undergoing Percutaneous Coronary Intervention.

Background: Ceramide, a key molecule in sphingolipid metabolism, is recognized as a standalone predictor of long-term major adverse cardiac events (MACE). We explore if integrating the global registry of acute coronary events (GRACE) score with the ceramide risk score (ceramide test 1, CERT1) improves MACE prediction in patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI).

Methods: This cohort study included 210 participants with ACS undergoing PCI.

Identification of biomarkers for knee osteoarthritis through clinical data and machine learning models.

Knee osteoarthritis (KOA) represents a progressive degenerative disorder characterized by the gradual erosion of articular cartilage. This study aimed to develop and validate biomarker-based predictive models for KOA diagnosis using machine learning techniques. Clinical data from 2594 samples were obtained and stratified into training and validation datasets in a 7:3 ratio.

Manganese is a potent inducer of lysosomal activity that inhibits de novo HBV infection.

Sodium taurocholate co-transporting polypeptide (NTCP) has been identified as an entry receptor for hepatitis B virus (HBV), but the molecular events of the viral post-endocytosis steps remain obscure. In this study, we discovered that manganese (Mn) could strongly inhibit HBV infection in NTCP-reconstituted HepG2 cells without affecting viral replication. We therefore profiled the antiviral effects of Mn2+ in an attempt to elucidate the regulatory mechanisms involved in early HBV infection.

Functional Analysis of Gene Mutation Leading to Maple Leaf Type in Cucumber ( L.).

The leaf morphology is an important agronomic trait in crop production. Our study identified a maple leaf type () cucumber mutant and located the regulatory gene for leaf shape changes through BSA results. Hybrid F1 and F2 populations were generated by F1 self-crossing, and the candidate genes were identified within the 2.

Augmented epigenetic repression of hepatitis B virus covalently closed circular DNA by interferon-α and small-interfering RNA synergy.

Unlabelled: The persistence of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is a key obstacle for HBV cure. This study aims to comprehensively assess the effect of interferon (IFN) and small-interfering RNA (siRNA) combination on the cccDNA minichromosome. Utilizing both cell and mouse cccDNA models, we compared the inhibitory effects of IFNα, siRNA, and their combination on cccDNA activity and assessed its epigenetic state.

Flotation separation of pyrite and carbonate minerals from a carlin-type gold deposit in Guizhou by a new combined collector and its adsorption mechanism.

Flotation is the most common method for effective separation of pyrite and carbonate minerals from Carlin-type gold deposits. The effect of conventional xanthate collector on flotation of a Carlin-type gold mine in Guizhou province is poor, and it can not effectively remove carbonate minerals. In this paper, butyl xanthoxanthate and benzohydroxamic acid (BHA) were used as a new combined collector for the flotation separation of a Carlintype gold mine in Guizhou Province.

A Sustainable and Eco-Friendly Membrane for PEM Fuel Cells Using Bacterial Cellulose.

Bacterial cellulose (BC) is an advantageous polymer due to its renewable nature, low cost, environmental compatibility, biocompatibility, biodegradability, chemical stability, and ease of modification. With these advantages, BC is an interesting candidate for the development of novel eco-friendly materials for proton-exchange membrane (PEM) applications. However, its practical applications have been limited by its relatively high dispersion in water, which usually occurs during the operation of proton-exchange membrane fuel cells (PEMFCs).

Palladium Single Atom-supported Covalent Organic Frameworks for Aqueous-phase Hydrogenative Hydrogenolysis of Aromatic Aldehydes via Hydrogen Heterolysis.

Developing a method for the tandem hydrogenative hydrogenolysis of bio-based furfuryl aldehydes to methylfurans is crucial for synthesizing sustainable biofuels and chemicals; however, it poses a challenge due to the easy hydrogenation of the C=C bond and difficult cleavage of the C-O bond. Herein, a palladium (Pd) single-atom-supported covalent organic framework was fabricated and showed a unique 2,5-dimethylfuran yield of up to 98.2 % when reacted with 5-methyl furfuryl aldehyde in an unprecedented water solvent at 30 °C.

Dual Modification for Low-Strain Ni-Rich Cathodes Toward Superior Cyclability in Pouch Full Cells.

Rapid capacity fading, interfacial instability, and thermal runaway due to oxygen loss are critical obstacles hindering the practical application and commercialization of Ni-rich cathodes (LiNiCoMnO, NCM811). Herein, a Sn/F codoping and LiF-coated Ni-rich cathode, denoted as NCM811-SF, is structurally fabricated that demonstrates very high cyclic and thermal stabilities. The introduction of Sn regulates the local electronic structure and facilitates the conversion of the layered structure into a spinel phase; F captures lithium impurities to form LiF coatings and forms TM-F bonds to reduce Ni/Li disordering.

The Cre/loxP-Based Recombinant HBV cccDNA System In Vitro and In Vivo.

Covalently closed circular DNA (cccDNA) exists as a stable episomal minichromosome in the nucleus of hepatocytes and is responsible for hepatitis B virus (HBV) persistence. We recently reported a technique involving recombinant cccDNA (rcccDNA) of HBV by site-specific DNA recombination. A floxed monomeric HBV genome was engineered into a precursor plasmid (prcccDNA) which was excised via Cre/loxP-mediated DNA recombination to form a 3.

Role of S100β in Unstable Angina Pectoris: Insights from Quantitative Flow Ratio.

Background And Objective: Disturbed autonomic nervous system (ANS) may promote inflammatory, immune, and oxidative stress responses, which may increase the risk of acute coronary events. S100β has been proposed as a biomarker of neuronal injury that would provide an insightful understanding of the crosstalk between the ANS, immune-inflammatory cells, and plaques that drive atherosclerosis. This study investigates the correlation between S100β, and functional coronary stenosis as determined by quantitative flow ratio (QFR).

The significance of CD4+ and CD8+ T lymphocyte infiltration in esophageal squamous cell carcinoma.

Purpose: To investigate the relationship between the abundance of CD4+ and CD8+ T cells in the tumor microenvironment and the prognosis of patients with esophageal squamous cell carcinoma, and to analyze their correlation and explore its clinical value.

Materials And Methods: In total, we enrolled 120 cases of esophageal squamous cell carcinoma diagnosed. The abundance of CD4+ and CD8+ T lymphocytes in the tissue specimens of esophageal cancer was examined by immunohistochemistry.

Treatment of lumbar disc herniation with robot combined with unilateral biportal endoscopic technology: A case report.

Background: This reported procedure combines the orthopedic surgical robot with the unilateral biportal endoscopy-lumbar interbody fusion (UBE-LIF), utilizing the UBE's wide viewing field and operating space to perform minimally invasive decompressive fusion of the lesioned segment, and the orthopedic surgical robot's intelligence and precision to perform percutaneous pedicle screw placement. The advancement of this procedure lies in the superposition of advantages and offsetting disadvantages of the two new technologies, and the maximum effect of treatment is achieved with maximum minimization of invasiveness and precision under the monitoring of imaging instruments to maximize the benefit of patients, and this review reports a case of multiple-segment lumbar decompression and fusion surgery for lumbar disc herniation robot-assisted UBE for reference.

Case Summary: A 44-year-old patient presented to our hospital.

Distinct serum exosomal miRNA profiles detected in acute and asymptomatic dengue infections: A community-based study in Baiyun District, Guangzhou.

Background: In recent years, research on exosomal miRNAs has provided new insights into exploring the mechanism of viral infection and disease prevention. This study aimed to investigate the serum exosomal miRNA expression profile of dengue-infected individuals through a community survey of dengue virus (DENV) infection.

Methods: A seroprevalence study of 1253 healthy persons was first conducted to ascertain the DENV infection status in Baiyun District, Guangzhou.

Development of a Cell Culture Model for Inducible SARS-CoV-2 Replication.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces direct cytopathic effects, complicating the establishment of low-cytotoxicity cell culture models for studying its replication. We initially developed a DNA vector-based replicon system utilizing the CMV promoter to generate a recombinant viral genome bearing reporter genes. However, this system frequently resulted in drug resistance and cytotoxicity, impeding model establishment.