Sensitive and specific colorimetric detection of glyphosate utilizing laccase-mimicking activity of copper-based metal-organic framework.

Glyphosate (Gly), a widely used potent herbicide worldwide, possess potential harm to human health and environment due to accumulation in water and soil. Hence, there is a pressing need to realize the simple and precise detection of Gly to ensure human health and environmental safety. Although various nanozyme-based detection methods have been developed for Gly, they frequently require additional enzymes and hydrogen peroxide (HO).

Novel mutations related to simultaneous congenital nephropathy and ovarian insufficiency.

Nucleoporins, as major components of nuclear pore complex, have been recently discovered to participate in organ development. Here, we report a young female patient with nephrotic proteinuria resistant to immune suppressant treatment and congenital ovarian insufficiency. Renal pathology confirmed focal segmental glomerulosclerosis and whole-exome sequencing revealed compound heterozygous mutations in Nucleoporin 160 (), NM_015231.

UDA-seq: universal droplet microfluidics-based combinatorial indexing for massive-scale multimodal single-cell sequencing.

The use of single-cell combinatorial indexing sequencing via droplet microfluidics presents an attractive approach for balancing cost, scalability, robustness and accessibility. However, existing methods often require tailored protocols for individual modalities, limiting their automation potential and clinical applicability. To address this, we introduce UDA-seq, a universal workflow that integrates a post-indexing step to enhance throughput and systematically adapt existing droplet-based single-cell multimodal methods.

Preparing the developing world for the next pandemic: Evidence from China's R&D blueprint for emerging infectious diseases.

Background: With double pressures of endemic and imported emerging infectious diseases (EIDs), China's ability to detect, prevent and control the unknown virus is of regional and global interest. This study aimed to establish an R&D Blueprint for EIDs in China by identifying the list of prioritized diseases and medical countermeasures (MCMs) that need proactive actions for the next pandemic.

Methods: The process mainly referred to the World Health Organization's prioritization methodology, supplemented by pipeline landscape, rapid risk assessment and multi-dimensional analysis.

Diversity-oriented synthesis of indole-fused scaffolds and bis(indolyl)methane from tosyl-protected tryptamine.

An efficient, diversity-oriented synthesis of indole-1,2-fused 1,4-benzodiazepines, tetrahydro-β-carbolines, and 2,2'-bis(indolyl)methanes was established starting from tosyl-protected tryptamine. These diverse privileged skeletons were controllably constructed by adjusting different hydride donors and Brønsted acids. A variety of indole-1,2-fused 1,4-benzodiazepines were facilely accessed using benzaldehydes bearing cyclic amines as hydride donors a cascade -alkylation/dehydration/[1,5]-hydride transfer/Friedel-Crafts alkylation sequence.

Genetic and clinical characterization of familial renal glucosuria.

Background: Familial renal glucosuria (FRG) is a hereditary disorder caused by variants in encoding sodium-glucose cotransporter 2 (SGLT2). In this study, we aimed to characterize proximal tubule solute transport, glucagon secretion and the genotype-phenotype relationship in FRG patients.

Methods: We sequenced and in 21 FRG patients and measured the renal threshold of glucose (RT) in 15 patients.

Construction of bacterial laccase displayed on the microbial surface for ultrasensitive biosensing of phenolic pollutants with nanohybrids-enhanced performance.

Although bacterial laccase (BLac) has many advantages including short fermentation period and adaptable activity to wide temperature and pH ranges, it is of challenge and significance to apply BLac to the biosensors, due to the intracellular secretion and poor electron transfer efficiency of BLac. Here, cell surface-displayed BLac (CSDBLac) was successfully constructed as whole-cell biocatalyst through microbial surface display technology, eliminating the mass transfer restriction and laborious purification steps. Meanwhile, MXenes/polyetherimide-multiwalled carbon nanotubes (MXenes/PEI-MWCNTs) nanohybrids were designed to immobilize CSDBLac and improve their electrochemical activity.

Frequent SLC12A3 mutations in Chinese Gitelman syndrome patients: structure and function disorder.

Purposes: This study was conducted to identify the frequent mutations from reported Chinese Gitelman syndrome (GS) patients, to predict the three-dimensional structure change of human Na-Cl co-transporter (hNCC), and to test the activity of these mutations and some novel mutations in vitro and in vivo.

Methods: SLC12A3 gene mutations in Chinese GS patients previously reported in the PubMed, China National Knowledge Infrastructure, and Wanfang database were summarized. Predicted configurations of wild type (WT) and mutant proteins were achieved using the I-TASSER workplace.

Decreased urinary uromodulin is potentially associated with acute kidney injury: a systematic review and meta-analysis.

Background: Urinary uromodulin (uUMOD) is one of the novel biomarkers for predicting AKI. However, currently available publications showed inconsistent results. We designed this meta-analysis to evaluate the potential association between uUMOD and AKI.

Adenosine A1 Receptor Deficiency Aggravates Extracellular Matrix Accumulation in Diabetic Nephropathy through Disturbance of Peritubular Microenvironment.

Background: We previously observed that adenosine A1 receptor (A1AR) had a protective role in proximal tubular megalin loss associated with albuminuria in diabetic nephropathy (DN). In this study, we aimed to explore the role of A1AR in the fibrosis progression of DN.

Methods: We collected DN patients' samples and established a streptozotocin-induced diabetes model in wild-type (WT) and A1AR-deficient (A1AR) mice.

Redox-Triggered Switchable Synthesis of 3,4-Dihydroquinolin-2(1)-one Derivatives via Hydride Transfer/-Dealkylation/-Acylation.

The switchable synthesis of 3-non, 3-mono, 3,3'-disubstituted 3,4-dihydroquinolin-2(1)-ones was developed through a redox-neutral hydride-transfer/-dealkylation/-acylation strategy from -aminobenzaldehyde with 4-hydroxycoumarin, and Meldrum's acid, respectively. The unprecedented strategy for the synthesis of 3,3'-highly functionalized 3,4-dihydroquinolin-2(1)-one has been realized with the in situ utilization of the released HCHO via the -QM involved Michael addition. In addition, the synthetic utility of this protocol has been well illustrated via concise synthesis of CYP11B2 inhibitor.

Hydrogen-bonding-assisted redox-neutral construction of tetrahydroquinolines via hydride transfer.

The hydrogen-bonding-assisted construction of tetrahydroquinolines decorated with structurally diverse 3,3'-difunctional groups has been realized via a hydride transfer-involved three-step cascade reaction in the presence of morpholine. This protocol solves the limitation of acyclic 1,3-dicarbonyl compounds by one-pot synthesis of tetrahydroquinolines, featuring operational simplicity, broadly applicable substrates, and metal- and acid-free conditions with EtOH as a hydrogen-bonding donor.

Fluorinated alcohol mediated N,N'-dialkylation of amino acid derivatives via cascade [1,5]-hydride transfer/cyclization for concise synthesis of tetrahydroquinazoline.

Structurally diverse amino acids and their ester derivatives were conveniently N,N'-dialkylated via a TFE-promoted cascade condensation/[1,5]-hydride transfer/cyclization for straightforward construction of pharmeutically significant tetrahydroquinazolines incorporating various amino acids, which featured broad substrate scope, the use of TFE as a sole solvent, additive-free and mild conditions.

The effect of A1 adenosine receptor in diabetic megalin loss with caspase-1/IL18 signaling.

Purpose: In our previous study, exacerbation of albuminuria was observed in A1 adenosine receptor knockout (A1AR) mice with diabetic nephropathy (DN), but the mechanism was unclear. Here, we investigated the relationship of megalin loss and albuminuria, to identify the protective effect of A1AR in megalin loss associated albuminuria by inhibiting pyroptosis-related caspase-1/IL-18 signaling of DN.

Methods: We successfully collected DN patients' samples and built diabetes mice models induced by streptozotocin.

Controllable Syntheses of Spiroindolenines and Benzazepinoindoles via Hexafluoroisopropanol-Mediated Redox-Neutral Cascade Process.

The pharmaceutically intriguing spiroindolenines incorporating tetrahydroquinoline were constructed via a hexafluoroisopropanol-promoted redox-neutral cascade cyclization from readily available starting materials. The benzazepinoindole skeletons could also be facilely accessed via one-pot sequential operation. Distinctive features of these transformations include their controllable access of the two privileged skeletons, high efficiency, simple operation, and mild reaction conditions.

Catalytic Formal Benzylic C-H Bond Functionalization of 2,5-Dialkylfuran Derivatives with Ferrocenyl Alcohols as Alkylation Reagents.

The inert benzylic C-H bond of π-electron-rich heteroaromatic 2,5-dialkylfuran derivatives was conveniently functionalized with ferrocenyl alcohols as alkylation reagents under catalytic acidic conditions at room temperature, which features chemo- and regiospecificity, mild and metallic catalyst-free conditions, and environmental benignity.

S1-Type Alkylation of N-Heteroaromatics with Alcohols.

The organocatalytic alkylation of 2-methyl-N-heteroaromatics with alcohols has been achieved via S1-type C(sp)-H functionalization, providing a green and efficient synthesis of indole and ferrocene-functionalized N-heteroaromatics in high yields.

Magnetic mesoporous thiourea-formaldehyde resin as selective adsorbent: A simple and highly-sensitive electroanalysis strategy for lead ions in drinking water and milk by solid state-based anodic stripping.

A simple and sensitive electroanalysis method has been developed for the direct determination of lead ions of nanomolar levels in real samples of drinking water and milk by employing magnetic mesoporous thiourea-formaldehyde resin (TUF@FeO) nanocomposites as the capturing absorbents. Here, the prepared TUF@FeO with the large-surface-area mesoporous structure and strong Pb-binding ligands could facilitate the selective and large-scale adsorption of Pb ions from the complex sample matrices to be further magnetically separated onto the magnetic electrodes. Moreover, the Pb ions magnetically accumulated were electrochemically measured alternatively by the solid state-based anodic stripping of PbCl.

Effects of sodium-glucose co-transporter 2 (SGLT2) inhibitors on serum uric acid level: A meta-analysis of randomized controlled trials.

The aim of this study was to describe the effects of sodium-glucose co-transporter 2 (SGLT2) inhibitors on serum uric acid (SUA) in patients with type 2 diabetes mellitus (T2DM). PubMed, CENTRAL, EMBASE and ClinicalTrials.gov were searched for randomized controlled trials of SGLT2 inhibitors in patients with T2DM up to May 20, 2017.

t-BuOK-Mediated Oxidative Dehydrogenative C(sp)-H Arylation of 2-Alkylazaarenes with Nitroarenes.

The first transition-metal free and regioselective C(sp)-H arylation of 2-alkylazaarenes with nitroarenes has been achieved via t-BuOK-mediated dehydrogenative C(sp)-C(sp) coupling. This reaction provides an efficient access to the biologically important and synthetically useful 2-benzyl-substituted azaarenes under mild conditions without the need of prefunctionalization of 2-alkylazaarenes or using the specialized arylating agents.

Effects of sodium-glucose co-transporter 2 (SGLT2) inhibition on renal function and albuminuria in patients with type 2 diabetes: a systematic review and meta-analysis.

Aim: To evaluate the effects of sodium-glucose co-transporter 2 (SGLT2) inhibition on renal function and albuminuria in patients with type 2 diabetes.

Methods: We conducted systematic searches of PubMed, Embase and Cochrane Central Register of Controlled Trials up to June 2016 and included randomized controlled trials of SGLT2 inhibitors in adult type 2 diabetic patients reporting estimated glomerular filtration rate (eGFR) and/or urine albumin/creatinine ratio (ACR) changes. Data were synthesized using the random-effects model.

Fluorinated alcohol-mediated [4 + 3] cycloaddition reaction of indolyl alcohols with cyclopentadiene.

This paper describes an efficient [4 + 3] cycloaddition reaction of 3-indolylmethanols with cyclopentadiene in hexafluoroisopropanol (HFIP), which is catalyst-free and inexpensive, and offers mild reaction conditions, wide substrate scope and convenient workup. This methodology provides the first catalyst- and additive-free [4 + 3] cycloaddition reactions of indolyl alcohols, offering a green and efficient method for the synthesis of cyclohepta[b]indole derivatives.