ODC-Driven Polyamines Synthesis Sustains the Self-Renewal of Glioblastoma Stem Cells and Drives Tumor Aggressiveness.

Glioblastoma stem cells (GSCs) are crucial drivers of tumor progression and therapeutic resistance in glioblastoma multiforme (GBM), yet the molecular mechanisms maintaining their self-renewal remain incompletely understood. Here, we identify a critical role for polyamine metabolism in GSCs maintenance. We show that GSCs exhibit elevated polyamine levels compared to non-stem tumor cells, attributed to enhanced ornithine decarboxylase (ODC) expression.

Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants.

Polyphenol oxidase (PPO) is a metalloproteinase widely present in plant organelles that plays crucial roles in photosynthesis, pest and disease resistance, growth and development, and flower color formation. Due to the high cost and reuse difficulties of plant PPO in applications, immobilization has emerged as a key technology to improve its stability, recyclability, and reusability. Immobilized plant PPO has been widely used in environmental and detection fields.

Construction and validation of a urinary stone composition prediction model based on machine learning.

The composition of urinary calculi serves as a critical determinant for personalized surgical strategies; however, such compositional data are often unavailable preoperatively. This study aims to develop a machine learning-based preoperative prediction model for stone composition and evaluate its clinical utility. A retrospective cohort study design was employed to include patients with urinary calculi admitted to the Department of Urology at the Second Affiliated Hospital of Zhengzhou University from 2019 to 2024.

Refinement of Crystalline Domains: A Strategy To Toughen Conductive Hydrogels.

The characteristic of possessing both high strength and flexibility is a requisite for the extensive application of conductive hydrogels in the domains of flexible wearable electronic devices and implantable biomedical fields. Nevertheless, the existing strategies for optimizing mechanical properties invariably enhance the strength at the expense of stretchability, thereby causing the hydrogels to still possess relatively low toughness. Herein, we propose a crystalline refinement-driven toughening strategy that overcomes this paradigm, achieving strength of 13.

Mitochondrial Calcium Uniporter Links Acetyl-CoA Metabolism and H3K27 Acetylation to Maintain Glioblastoma Stem Cells.

Glioblastoma stem cells (GSCs) exhibit remarkable metabolic and epigenetic adaptability, contributing to therapeutic resistance and tumor recurrence. The mechanisms underlying this plasticity represent potential targetable vulnerabilities to improve glioblastoma treatment. Here, we identified a critical metabolic-epigenetic axis centered on the mitochondrial calcium uniporter (MCU) that governs GSC survival and tumor initiation.

NSD2 promotes cell durotaxis and drives the transition from polycystic kidney disease to tubulocystic renal cell carcinoma through integrin/FAK/AKT signaling.

Renal cell carcinoma (RCC) is one of the most common malignancies in the urinary system. NSD2 is an H3K36-specific di-methyltransferase that has been reported to participate in diverse biological processes and human tumors. However, its role in RCC remains unclear.

Extract of camellia seed cake ameliorates glycolipid metabolism disorder in mice through inhibiting ACOX1 activity.

Acyl-CoA oxidase 1 (ACOX1) plays a key role in glycolipid metabolism disorders. Camellia seed cake, a byproduct of oil production, was identified as a source of ACOX1 inhibitors. Under optimized conditions (58 % ethanol, 70 °C, 62 min), the extract exhibited an 83.

Evaluation of cosmetic shoulder balance in Lenke type 1 and 2 adolescent idiopathic scoliosis across different observational perspectives.

Background: Shoulder imbalance in patients with adolescent idiopathic scoliosis (AIS) contributes to a negative self-image and psychological distress, particularly in those with Lenke type 1 or 2 curves. Although previous studies have emphasized the significance of cosmetic shoulder balance (Sh.B) in informing clinical decisions and evaluating postoperative patient-reported outcomes (PROs), the observational perspective-a crucial factor-has often been overlooked in cosmetic assessment, potentially leading to inaccuracies and inconsistencies in measurements.

Epigallocatechin gallate prevents and alleviates type 2 diabetes mellitus (T2DM) through gut microbiota and multi-organ interactions in Wistar healthy rats and GK T2DM rats.

Introduction: As the main active ingredient of the first FDA-approved phytochemical drug, epigallocatechin gallate (EGCG) can effectively alleviate glucolipid metabolic disorders. However, existing studies mainly focuses on the treatment of EGCG in disease models, with limited focus on its preventive effect on diseases in healthy models.

Objectives: This study investigated how EGCG prevents and alleviates T2DM through gut microbiota and multi-organ interactions in Wistar healthy rats and GK T2DM rats.

Assembling a Metastable Electron Fence within Gold-Zeolite Interfaces for Boosted Propylene Epoxidation.

Selective oxidation of hydrocarbons represents a cornerstone reaction in the chemical industry, yet achieving both high activity and selectivity remains challenging. Gold catalysts, renowned for their resistance to overoxidation, are hindered by poor oxygen activation. Here, we develop an "electron fence" strategy to overcome these limitations and enhance the oxidation performances of a conventional gold/zeolite catalyst, which achieves a record-breaking propylene epoxidation rate of 502.

Advancements in Gene-Based Therapeutic Angiogenesis for Chronic Limb-Threatening Ischemia.

The objective of this article is to summarize the research progress and discuss the current difficulties of gene-based therapeutic angiogenesis in lower limb ischemic diseases, so as to provide new research directions for the non-invasive treatment of lower limb ischemia. The basic and clinical trials of gene-based therapeutic angiogenesis in lower limb ischemia in recent years were read and reviewed. Growth factors such as vascular endothelial growth factor, hepatocyte growth factor, and fibroblast growth factor have been extensively studied for their application in lower limb ischemic diseases.

The Extract of Camellia Seed Cake Alleviates Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in Mice by Promoting Coenzyme Q Synthesis.

: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent metabolic disorder. Camellia seed cake, a byproduct of oil extraction, contains a variety of bioactive compounds. This study investigated the regulatory effects and underlying mechanisms of camellia seed cake extract (CSCE) using a high-fat diet (HFD)-induced MASLD mouse model.

Seltoplasmid promotes ulcer healing versus placebo for treating patients with chronic limb-threatening ischemia: HOPE CLTI-2 trial.

Intramuscular injection of donaperminogene seltoplasmid (recombinant human hepatocyte growth factor plasmids) represents a gene therapy that treats patients with chronic limb-threatening ischemia (CLTI). The HOPE CLTI-2 trial was a phase 3, multicenter, double-blind, placebo-controlled study aimed to evaluate the efficacy and safety of seltoplasmid in patients with Rutherford class 5 CLTI. This study did not require participants to be ineligible for revascularization, allowing enrollment of patients with CLTI caused by either atherosclerosis (ASO) or Buerger disease (or thromboangiitis obliterans [TAO]).

Research Progress on the Protective Effect of Green Tea Polyphenol (-)-Epigallocatechin-3-Gallate (EGCG) on the Liver.

The liver, as the primary metabolic organ, is susceptible to an array of factors that can harm liver cells and give rise to different liver diseases. Epigallocatechin gallate (EGCG), a natural compound found in green tea, exerts numerous beneficial effects on the human body. Notably, EGCG displays antioxidative, antibacterial, antiviral, anti-inflammatory, and anti-tumor properties.

Optimal injection sites for therapeutic angiogenesis: HGF-mediated regulation of HIF-1α via MAPK/PI3K pathways in hypoxic endothelial cells.

Therapeutic angiogenesis offers a promising strategy for patients with critical limb-threatening ischemia (CLTI) who are unsuitable candidates for revascularization. However, the optimal administration sites for gene therapy agents, such as pCK-HGF-X7, remains undefined. Clinical trials commonly employ multiple intramuscular injections at sites of arterial occlusion; yet the necessity and efficacy of such extensive and repetitive protocols remains unclear.

New biological strategies for preventing and controlling food contaminants in the supply chain: Smart use of common plant-derived substances.

Traditional means of contaminant management that rely on chemical additives and high-temperature processing have raised concerns about long-term safety and environmental issues in the modern food supply chain. Therefore, sustainable, safe, and innovative strategies are urgently needed. Plant-derived substances are known for their biological activity and antifouling potential as natural alternatives for contamination control.

Comparative study of the anti-obesity effects of white tea and dark tea: Insights from microbiome and metabolomics.

The anti-obesity effects of tea and its functional components have been extensively studied. However, the protective effects of different types of tea against obesity induced by a high-fat diet (HFD) and the underlying mechanisms remain unclear. This study systematically compared the effects of white tea and dark tea on obese rats and explored their mechanisms.

Relationship Between Depression and Epigallocatechin Gallate from the Perspective of Gut Microbiota: A Systematic Review.

Depression, a serious mental illness, is characterized by high risk, high incidence, persistence, and tendency to relapse, posing a significant burden on global health. The connection between depression and gut microbiota is an emerging field of study in psychiatry and neuroscience. Understanding the gut-brain axis is pivotal for understanding the pathogenesis and treatment of depression.

Eurotium-Cristatum fermented black tea alleviates ulcerative colitis through the PPARγ-NF-κB signaling axis.

Ulcerative colitis (UC) is a chronic inflammatory bowel condition that significantly impairs patient quality of life and remains incurable. Effective dietary management is crucial for both prevention and treatment. This study investigates the effects and mechanisms of Eurotium cristatum-fermented black tea (FBT) in a dextran sulfate sodium (DSS)-induced UC mouse model using transcriptome sequencing, immunofluorescence, and flow cytometry.

Platelet Membrane-Coated HGF-PLGA Nanoparticles Promote Therapeutic Angiogenesis and Tissue Perfusion Recovery in Ischemic Hindlimbs.

Therapeutic angiogenesis has garnered significant attention as a potential treatment strategy for lower limb ischemic diseases. Although hepatocyte growth factor (HGF) has been identified as a key promoter of therapeutic angiogenesis, its clinical application is limited due to its short half-life. In this study, we successfully developed and characterized platelet membrane-coated HGF-poly(lactic--glycolic acid) (PLGA) nanoparticles (NPs).

Key Metabolites Influencing Astringency and Bitterness in Yinghong 9 Large-Leaf Dark Tea Before and After Pile-Fermentation.

Understanding the impacts of pile-fermentation on the taste quality of dark tea (DT) is crucial. Although the large-leaf DT, Yinghong 9 DT, was successfully developed, its taste quality was not systematically studied. This research aims to analyze how pile-fermentation affects taste.

Preliminary exploration of the C-3 galloyl group and the B-5' hydroxyl group enhance the biological activity of catechins in alleviating obesity induced by high-fat diet in mice.

Catechins, among the most active components in tea, effectively alleviate obesity. Catechins are primarily classified into four types based on the presence or absence of the C-3 galloyl group and the B-5' hydroxyl group. However, the impact of conformation on the anti-obesity properties of catechins remains unclear.

Graphene Microflower by Photothermal Marangoni-Induced Fluid Instability for Omnidirectional Broadband Photothermal Conversion.

2-D carbon-based materials are well-known for their broadband absorption properties for efficient solar energy conversion. However, their high reflectivity poses a challenge for achieving efficient omnidirectional light absorption. Inspired by the multilevel structures of the flower, a Graphene Microflower (GM) material with gradient refractive index surface was fabricated on polymer substrates using the UV-intense laser-induced phase explosion technique under the synergistic design of the photothermal Marangoni effect and the fluid instability principle.