DL-CSPF: deep-learning-based cell segmentation with a physical framework for digital holographic microscopy.

Digital holographic microscopy (DHM) offers label-free, full-field imaging of live-cell samples by capturing optical path differences to produce quantitative phase images. Accurate cell segmentation from phase images is crucial for long-term quantitative analysis. However, complicated cellular states (e.

Thallium-Doping-Modulated Photon Emission and Scintillation Performance in CsCuI Nanocrystals.

Fast and low-dose X-ray imaging is crucial for medical and security applications. Tl-doped CsCuI stands out for dynamic X-ray imaging, outperforming many commercial scintillators. However, the mechanisms underlying the scintillation enhancements achieved through Tl doping remain unclear, hindering precise optimization and restricting utilization of its potential.

Differential phase contrast intensity diffraction tomography.

Intensity diffraction tomography (IDT) is a label-free computational microscopy technique that infers 3D refractive index (RI) and absorption distributions of objects from intensity-only measurements. Nevertheless, the inherent coherent image formation model requires sequential intensity measurements under various plane wave illuminations, resulting in time-consuming data acquisition and low imaging speed. In this Letter, we propose differential phase contrast intensity diffraction tomography (DPC-IDT), which leverages partially coherent illumination to extend the accessible spectrum range, thereby achieving high-speed, motion-free 3D tomographic microscopy.

Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography.

Lens-free on-chip microscopy is a powerful and promising high-throughput computational microscopy technique due to its unique advantage of creating high-resolution images across the full field-of-view (FOV) of the imaging sensor. Nevertheless, most current lens-free microscopy methods have been designed for imaging only two-dimensional thin samples. Lens-free on-chip tomography (LFOCT) with a uniform resolution across the entire FOV and at a subpixel level remains a critical challenge.

Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy.

We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of thick biological samples.

In Situ Fabrication of CsCuI: Tl Nanocrystal Films for High-Resolution and Ultrastable X-ray Imaging.

CsCuI nanocrystals (NCs) are considered to be promising materials due to their high photoluminescence efficiency and X-ray hardness. However, the present strategy depends on tedious fabrication with excessive chemical waste. The evasive iodide ion dissociation, inadaptable ligand system, low stability, and relatively low light yield severely impede their applications.

Accurate quantitative phase imaging by the transport of intensity equation: a mixed-transfer-function approach: erratum.

In this erratum, we correct Fig. 4 of our Letter [Opt. Lett.

Wavelength-scanning lensfree on-chip microscopy for wide-field pixel-super-resolved quantitative phase imaging.

We propose a lensfree on-chip microscopy approach for wide-field quantitative phase imaging (QPI) based on wavelength scanning. Unlike previous methods, we found that a relatively large-range wavelength diversity not only provides information to overcome spatial aliasing of the image sensor but also creates sufficient diffraction variations that can be used to achieve motion-free, pixel-super-resolved phase recovery. Based on an iterative phase retrieval and pixel-super-resolution technique, the proposed wavelength-scanning approach uses only eight undersampled holograms to achieve a half-pitch lateral resolution of 691 nm across a large field-of-view of 29.

Accurate quantitative phase imaging by the transport of intensity equation: a mixed-transfer-function approach.

As a well-established deterministic phase retrieval approach, the transport of intensity equation (TIE) is able to recover the quantitative phase of a sample under coherent or partially coherent illumination with its through-focus intensity measurements. Nevertheless, the inherent paraxial approximation limits its validity to low-numerical-aperture imaging and slowly varying objects, precluding its application to high-resolution quantitative phase imaging (QPI). Alternatively, QPI can be achieved by phase deconvolution approaches based on the coherent contrast transfer function or partially coherent weak object transfer function (WOTF) without invoking paraxial approximation.

[Spatial and Temporal Distribution Characteristics and the Retention Effects of Nutrients in Xiangjiaba Reservoir].

After the construction of the Xiangjiaba Dam, the hydrodynamic conditions, nutrient distributions, and transport conditions of the Jinsha River were changed. Here, the nutrient distribution characteristics and retention effects of Xiangjiaba Reservoir were investigated according to the results of water quality monitoring from 2015 to 2016. Spatial and temporal variations in TN, TP, SiOSi, and other nutrients, and retention flux and retention rate were analyzed.

Efficient quantitative phase microscopy using programmable annular LED illumination.

In this work, we present an efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination. As a new type of coded light source, the LED array provides flexible illumination control for noninterferometric QPI based on a traditional microscopic configurations. The proposed method modulates the transfer function of system by changing the LED illumination pattern, which provides noise-robust response of transfer function and achieves twice resolution limit of objective NA.

Extent of agreement between the body fluid model of Sysmex XN-20 and the manual microscopy method.

Background: Although the correlations concerning cellular component analysis between the Sysmex XN-20 body fluid (BF) model and manual microscopy have been investigated by several studies, the extent of agreement between these two methods has not been investigated.

Methods: A total of 90 BF samples were prospectively collected and analyzed using the Sysmex XN-20 BF model and microscopy. The extent of agreement between these two methods was evaluated using the Bland-Altman approach.