Applications and challenges of DNA barcoding in rapid radiation groups: (Crassulaceae) as a case study.

Objective: et (Hongjingtian in Chinese, RCRR), the roots and rhizomes of and its application in the medicinal market is very chaotic. In this study, DNA barcoding database and identification engine of species were established, decoction pieces from the medicinal market were identified, and the application and challenges of DNA barcoding in the rapid radiation of species were analyzed. This study provides reference for the protection, rational development, and utilization of endangered resources within species.

Lutein and Astaxanthin Supplementation Induce Competitive Inhibition of Carotenoid Deposition in Egg Yolk.

Lutein- and astaxanthin-fortified eggs can greatly improve both nutritional and economic values of eggs. High doses of lutein and astaxanthin are often supplemented in hen diets to rapidly accumulate lutein and astaxanthin in eggs. In this study, an additional 100, 200, and 400 mg/kg lutein and astaxanthin were added in hen diets to explore the effects of high-dose lutein and astaxanthin on egg quality, egg production, and carotenoid deposition in eggs, which remains unclear.

Self-improving generative foundation model for synthetic medical image generation and clinical applications.

In many clinical and research settings, the scarcity of high-quality medical imaging datasets has hampered the potential of artificial intelligence (AI) clinical applications. This issue is particularly pronounced in less common conditions, underrepresented populations and emerging imaging modalities, where the availability of diverse and comprehensive datasets is often inadequate. To address this challenge, we introduce a unified medical image-text generative model called MINIM that is capable of synthesizing medical images of various organs across various imaging modalities based on textual instructions.

Endoscopic retrograde appendicitis therapy.

Endoscopic retrograde appendicitis therapy (ERAT) is a novel and minimally invasive technological alternative for the management of acute or chronic appendicitis. Through endoscopic appendiceal intubation, obstructions such as appendiceal feces and parasites within the appendiceal lumen can be effectively eliminated, leading to patient recovery. Additionally, in cases where the orifices are swollen or complicated appendicitis is present, a stent may be inserted following appendiceal flushing.

Complete mitochondrial DNA sequence of Oka, 1910 (Rhynchobdellida: Glossiphoniidae) and its phylogenetic analysis.

The complete mitochondrial genome of (basionym: ), sourced from a biodiversity hotspot of China, has been determined and reported in this study. It was 15,236 bp in length and consisted of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and three control regions. The mitogenome was deposited GenBank under the accession number PP165800.

Modified endoscopic retrograde appendicitis therapy vs. laparoscopic appendectomy for uncomplicated acute appendicitis in children.

Objectives: Modified endoscopic retrograde appendicitis therapy (mERAT) has been proposed as an alternative to laparoscopic appendectomy for the treatment of appendicitis. However, data from children in large samples are lacking. The aim of this article is to evaluate the efficacy between mERAT and laparoscopic appendectomy (LA) in children with uncomplicated appendicitis.

The application value of high-frequency ultrasound in the feasibility assessment of endoscopic retrograde appendicitis therapy in children with appendicitis.

Acute appendicitis is one of the common acute abdominal diseases in pediatrics. However, the implementation of radiological examination guided endoscopic retrograde appendicitis therapy (ERAT) in adults is limited in children. Our previous research explored the non-invasive guidance of high-frequency ultrasound (HFUS) for ERAT and achieved good therapeutic effects.

Application value of high-frequency ultrasonography in endoscopic retrograde appendicitis therapy for pediatric acute appendicitis.

Background: Endoscopic retrograde appendicitis therapy (ERAT) is a new method of treating acute appendicitis that has emerged in recent years in children, but the application of radiological examination in the diagnosis and treatment of pediatric diseases is greatly limited. Therefore, high-frequency ultrasonography imaging has attracted more and more attention because of its advantages such as non-invasiveness, no radiation, and a simpler procedure. This study aims to explore the application value of high-frequency ultrasonography in ERAT for pediatric acute appendicitis.

[Application of ultrasound-guided endoscopic retrograde appendicitis therapy in children with appendix-related chronic abdominal pain].

Objectives: To study the clinical efficacy of ultrasound-guided endoscopic retrograde appendicitis therapy in children with appendix-related chronic abdominal pain.

Methods: A retrospective analysis was performed on the medical data of 30 children with the chief complaint of chronic abdominal pain who were admitted from August 2019 to May 2021. All the children were found to have inflammation of the appendix or intracavitary stool and fecalith by ultrasound and underwent ultrasound-guided endoscopic retrograde appendicitis therapy.

Health-Related Quality of Life and Influencing Factors of Pediatric Medical Staff During the COVID-19 Outbreak.

To evaluate the health-related quality of life (HRQoL) status and explore its associated factors in pediatric medical staff during the COVID-19 epidemic so as to provide fundamental evidence for clinicians and administrators to formulate targeted intervention measures to improve the HRQoL and mental health status in pediatric medical staff during this, and future pandemics. A cross-sectional study was conducted to investigate the HRQoL of pediatric medical staff. Univariable and multivariable logistic regression were used to analyze the associated factors.

The modified endoscopic retrograde appendicitis therapy versus antibiotic therapy alone for acute uncomplicated appendicitis in children.

Background: Endoscopic retrograde appendicitis therapy (ERAT) is an emerging endoscopic treatment modality for acute uncomplicated appendicitis (AUA) supported by several case series. However, to date, systematic studies have not been conducted in children and the prospective comparative data are lacking. Moreover, due to a concern for future malignancy risk in children from ionizing radiation, we used contrast-enhanced ultrasound (CEUS) instead of endoscopic retrograde appendiceal radiography (ERAR).

[The extracellular segment of mouse soluble SIRPα enhances the phagocytosis of macrophages to L1210 leukemia cells].

Objective To study the role of mouse extracellular segment protein of signal-regulatory protein α gene (mSIRPα) of mice in tumor immune regulation by cloning mSIRPα, constructing its prokaryotic expression vector and achieving the soluble expression of SIRPα. Methods The mSIRPα gene was amplified from mouse lymph node and the prokaryotic expression vector of pET32a-SIRPα was further constructed. After the soluble expression of recombinant Trx-mSIRPα fusion protein containing thioredoxin (Trx) tag was achieved, mouse bone marrow-derived monocytes were cultured and induced to differentiate into macrophages.

[Effect of eicosapentaenoic acid on mRNA expression of tight junction protein ZO-1 in intestinal epithelial cells after Escherichia coli LF82 infection].

Objective: To investigate the change in the expression of tight junction protein ZO-1 in intestinal epithelial cells (Caco-2 cells) and the protective effect of eicosapentaenoic acid (EPA) after adherent-invasive Escherichia coli (E.coli) LF82 infection.

Methods: The Caco-2 cell line was used to establish an in vitro model of tight junction of intestinal epithelial cells.