Decoding the Sleep Enigma: A Proteomic Mediation Analysis Linking Healthy Sleep Patterns to Lung Cancer Risk.

Study Objectives: The biological link between sleep patterns and lung cancer remains an enigma. This study aims to explore how healthy sleep patterns modulate lung cancer risk through proteomic mediation, and develop a sleep-related biomarker panel.

Methods: This study utilized data from the UK Biobank, comprising 34,881 participants.

RPA1 protects DNA damage-induced PANoptosis in limb development.

Extensive cell proliferation during embryogenesis often compromises genome integrity, increasing the risk of developmental defects. However, the mechanisms that safeguard genome integrity during this process remain poorly understood. Using early limb development as a model, we identify that DNA damage response factors are up-regulated in proliferating mesenchymal stem cells.

Coexistence of hepatitis B e antigen and antibody during antiviral treatment predicts better clinical outcomes in children.

Objectives: The coexistence of hepatitis B e antigen (HBeAg) and hepatitis B e antibody (HBeAb) during antiviral therapy is considered atypical in patients with chronic hepatitis B (CHB), and its clinical implications remain inadequately understood, particularly in pediatric patients. This study aimed to investigate the clinical characteristics of this coexistence pattern and its impact on the outcomes of combined antiviral therapy in children with CHB.

Methods: A total of 254 treatment-naïve children diagnosed with HBeAg-positive CHB were retrospectively enrolled.

Adaptive cationic peptide bundles hydrogel for the repair of infected mandibular defects.

Due to bacterial resistance and the limited self-healing capacity, the treatment of infected mandibular defects remains great challenges. Cationic peptide bundles can kill bacteria more efficiently than the linear ones. However, they are hard to release from hydrogels because of the nonflexible structures, thus limiting its application.

Chondroitin sulfate protects against synaptic impairment caused by fluorosis through the Erk1/2-MMP-9 signaling pathway.

Prolonged exposure to fluoride may induce neurotoxic effects. Chondroitin sulfate (CS) exhibits protective functions within the central nervous system (CNS); however, the mechanism by which CS protects synapses against fluoride remains incompletely understood. Our objective was to investigate the protective efficacy of CS on synapses and decipher its underlying mechanisms.

Assessment of potential disease-drug interactions when prescribed Fuzi for rheumatoid arthritis treatment, with focus on the pharmacokinetics of aconitine, mesaconitine, and hypaconitine.

Ethnopharmacological Relevance: Fuzi is a toxic traditional Chinese medicine that is commonly prescribed for rheumatoid arthritis (RA), while CYP3A4 and P-gp are primarily responsible for the metabolism and efflux of its toxic alkaloids: aconitine (AC), mesaconitine (MA), and hypaconitine (HA). However, the RA condition may induce disease-drug interactions due to the elevated IL-6 and TNF-α levels, which significantly inhibit the function of CYPs and P-gp.

Aim Of The Study: To investigate the existence of disease-drug interactions during Fuzi treatment for RA, focusing on the pharmacokinetics of AC, MA, and HA.

Laparoscopic salvage in a storm: A multidisciplinary case report of thyroid storm complicated by gastrointestinal perforation.

Rationale: Thyroid storm (TS) is a critical endocrine emergency characterized by multiorgan failure. Although rare, gastrointestinal perforation is a catastrophic complication that frequently leads to diagnostic delays and poor prognosis. This case provides valuable clinical insights based on a multidisciplinary strategy and well-considered surgical timing.

Whole exome sequencing study of adamantinomatous craniopharyngioma reveals the mutational characteristics of recurrent cases.

Purpose: Adamantinomatous craniopharyngioma (ACP) is a benign but surgically challenging intracranial tumor, and its high postoperative recurrence rate poses a threat to patient prognosis. In this study, whole exome sequencing (WES) was performed to comprehensively understand the genomic alterations in ACP.

Methods: First, the gene mutation profile of ACP was acquired through an overview of the WES data.

Analysis of risk factors of unfavorable treatment outcomes in patients with rifampicin-sensitive pulmonary tuberculosis.

This study analyzes the risk factors of unfavorable treatment outcomes in rifampicin-sensitive pulmonary tuberculosis (PTB) patients. Clinical data of 694 patients with rifampicin-sensitive PTB admitted to Quzhou Hospital Affiliated to Wenzhou Medical University from January 2020 to December 2021 were retrospectively analyzed. Univariate and multivariate logistic regression were used to analyze the related risk factors, and the predictive value was assessed using the receiver operating characteristic curve.

Complex Alkaloid-Type Polycycles via Aza-Michael/Epoxidation of Indole-7-Carbaldehydes with Vinyl Sulfonium Salts and Alkenyl Thianthrenium Salts.

An efficient synthesis of pyrroloquinolines has been developed via the aza-Michael/epoxidation cascade process in a diastereospecific manner. The domino reaction occurred properly to afford the desired tetracyclic indoles in 50->99% isolated yields. This synthetic protocol displayed especially broad substrate generality with respect to both indole-7-carbaldehydes and vinylsulfonium salts.

The impact of early use of statin in sepsis patients with acute kidney injury: a study based on MIMIC-IV.

Objective: Sepsis-associated acute kidney injury (SA-AKI) is a prevalent and serious condition in the ICU. The efficacy of statin in these patients remains unclear. The purpose of this study was to investigate the impact of the early use of statin on the prognosis of SA-AKI patients.

A randomized controlled trial comparing novel and traditional apical barrier techniques in endodontic procedures.

Objectives: This study aimed to compare the clinical efficacy of a novel apical barrier technique (N-ABT) and a traditional apical barrier technique (T-ABT).

Materials And Methods: A randomized controlled clinical trial was conducted. Forty-seven patients with 50 teeth meeting the inclusion criteria of large root canals and incompletely developed apical foramina were enrolled.

Real-world respiratory effectiveness of mepolizumab in severe eosinophilic asthma and eosinophilic granulomatosis with polyangiitis.

Background: Severe eosinophilic asthma (SEA) and eosinophilic granulomatosis with polyangiitis (EGPA) share a common type 2 inflammatory pathway but differ in systemic involvement and corticosteroid dependence. Real-world data on mepolizumab's effectiveness in Chinese SEA and EGPA populations are limited, and no study has jointly evaluated both cohorts.

Methods: We conducted a single-center, retrospective cohort study of 38 consecutive SEA or EGPA patients treated with mepolizumab between July 2022 and February 2025 in China.

One-pot one-step detection platform for severe fever with thrombocytopenia syndrome virus via the CRISPR/Cas12a detection system.

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne virus that primarily causes SFTS. Although a common testing route is available, a timely, conventional and accurate method for SFTSV detection is urgently needed. In the present study, we established a platform that combines the recombinase polymerase amplification (RPA) assay with the clustered regularly interspaced short palindromic repeats-CRISPR associated proteins (CRISPR/Cas) 12a technique in one step in one pot.

Pharmacological mechanism and structure of ubiquitin proteases associated with depression: A review.

Depression is one of the most common neurologic diseases. It is associated with multiple factors such as inflammation, NMDAR, GABAR, and monoamine neurotransmitters. Ubiquitin and ubiquitination are responsible for protein degradation.

Effect of High Maintenance Dose Versus Low Dose of Remifentanil on Incidence of Postoperative Nausea and Vomiting (PONV) in Patients Under Gynecological Laparoscopic Procedure: A Pilot Study.

Purpose: The aim of this trial was to investigate postoperative nausea and vomiting (PONV) incidence between low- and high-dose remifentanil with propofol for total intravenous anaesthesia (TIVA) in adult female patients with gynecologic laparoscopy.

Patients And Methods: The randomized clinical trial consisted of two groups: 0.1ug/kg/min remifentanil (L group) (n=39) and 0.

Lineage Tracing Identified Cell Populations within Adult Stem Cell Niches for Oral Maxillofacial Hard Tissue Formation.

The regeneration of oral maxillofacial hard tissues is currently one of the issues of most concern in public health. This complex process involves a variety of cell types residing in a specialised microenvironment known as the adult stem cell niche in living organisms. Within this niche, adult stem cells are considered to play a central role in the regeneration of hard tissues, which undergo rapid proliferation and differentiation into progenitor cells to replace lost tissue, throughout postnatal life.

The role of endoplasmic reticulum aminopeptidase ERAP2 pathogenic mutation rs1363907 in amoxicillin clavulanate-induced liver injury: a special case report.

Idiosyncratic hepatotoxicity is a type of drug-induced liver injury (DILI) that is unpredictable and clinically severe, and amoxicillin clavulanate (AC) is the most implicated drug in DILI worldwide. The clinical manifestations of amoxicillin clavulanate-induced liver injury (AC-DILI) are fatigue and jaundice, which some allergic features may accompany, but autoimmune phenomena are uncommon. Here, we describe a special case report of a patient with AC-DILI accompanied by autoimmune phenomena for the first time.

Increased optic nerve stiffness can aid in localizing ipsilateral brain or eye lesions in patients with secondary headaches.

Background: Ultrasound (US) measurement of the optic nerve sheath diameter serves as a valuable adjunctive monitoring tool in acute care. However, the relationship between optic nerve stiffness assessed by shear wave elastography (SWE) and the presence of ipsilateral brain or eye lesions in patients with secondary headaches remains unclear. The aim of this study was to explore the association between optic nerve stiffness values and the presence of ipsilateral brain or eye lesions in patients with secondary headaches.

Sex-biased fertility degeneration induced by depletion of an auxiliary piRNA-pathway factor Qin in Bombyx mori.

The PIWI-interacting RNA (piRNA) pathway is the major defense system for transposable elements (TEs) silencing in animal gonads, maintaining genomic integrity of germ cells and ensuring proper gametogenesis. An the piRNA-pathway factor, Qin, has been reported to participate in piRNA biogenesis in the lepidopteran model insect, Bombyx mori. Nevertheless, the physiological functions of Qin remain to be characterized.

Isolation and Characterization of the Adamantinomatous Craniopharyngioma Primary Cells with Cancer-Associated Fibroblast Features.

: Adamantinomatous craniopharyngiomas (ACPs) are benign intracranial tumors that behave aggressively due to their location, infiltration of the surrounding nervous tissue and high capacity for recurrence. In this study, we aimed to construct ACP primary cell models for further investigation of tumorigenic and recurrent mechanisms. : Primary cells were isolated from primary (one case) and recurrent (one case) ACP.

Nuclear-Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance.

Homologous recombination repair (HRR) is crucial for maintaining genomic stability by repairing DNA damage. Despite its importance, HRR's role in cancer progression is not fully elucidated. Here, this work shows that nuclear-localized branched-chain α-ketoacid dehydrogenase kinase (BCKDK) acts as a modulator of HRR, promoting cell resistance against DNA damage-inducing therapy in breast cancer.

Acute pain service for postoperative pain in adults: a network meta-analysis.

Background: Postoperative pain significantly impacts patients' quality of life and recovery. Although acute pain services (APS) have been implemented in many hospitals worldwide, no study has directly compared the efficacy of different APS subtypes in managing acute postoperative pain.

Objective: This network meta-analysis aimed to evaluate the effectiveness of various APS models in alleviating postoperative pain in adults undergoing surgery.