Patient-reported outcomes and their associated factors at 1- and 2-year follow-up after lumbar spine surgery: a surgery registry study.

Purpose: Degenerative lumbar conditions are a leading cause of disability worldwide, often requiring surgery when conservative treatments fail. Data on surgical outcomes from patients' perspectives and influencing factors remain limited. This study aimed to assess 1-year and 2-year Patient-Reported Outcomes (PROs) following lumbar spine surgery and identify factors associated with these outcomes.

Developing an EQ-5D-5L Value Set for Singapore.

Objectives: The EQ-5D-5L is a multi-attribute utility instrument recommended by many health technology assessment agencies. This study aimed to develop an EQ-5D-5L value set for Singapore.

Methods: A 'lite' version of the EuroQol Research Foundation's EQ-5D-5L valuation protocol, which was designed to value a total of 91 health states using a composite time trade-off (cTTO) method, was followed.

Validation of the consumer health activation index (CHAI) among community-dwelling adults in primary care clinics in Singapore.

Background: Health activation is an individual's knowledge, skills, and confidence in managing personal health and healthcare. The Consumer Health Activation Index (CHAI) is a freely available, 10-item measure originally developed in the United States. This study aimed to validate CHAI among community-dwelling adults in Singapore, examining its content validity, construct validity and test-retest reliability.

Integrative analysis of gut microbiome and serum metabolomics explores the therapeutic mechanism of tongfeng qingxiao prescription in treating gouty arthritis.

Gouty arthritis (GA), a metabolic inflammatory disorder, shows increasing global prevalence, especially in China. Conventional GA treatments often cause adverse reactions, necessitating alternative therapies. Tongfeng Qingxiao Prescription (TFQXP) demonstrates clinical efficacy in GA, though its mechanisms remain unclear.

Efficacy and safety of upadacitinib as monotherapy or combined with elsubrutinib for the treatment of systemic lupus erythematosus: results through 104 weeks in a long-term extension study.

Objectives: This 1-year long-term extension (LTE) study (NCT04451772) followed the 48-week phase 2 SLEek study (NCT03978520) that evaluated upadacitinib (a Janus kinase inhibitor) alone or combined with elsubrutinib (a Bruton's tyrosine kinase inhibitor) in adults with moderately to severely active systemic lupus erythematosus (SLE). The objective was to evaluate the efficacy and safety of an additional 56 weeks of treatment.

Methods: Patients randomised to upadacitinib 30 mg one time per day (QD) or upadacitinib 30 mg/elsubrutinib 60 mg QD (upadacitinib/elsubrutinib) in the SLEek study continued their assigned treatment during the LTE.

Enamine-Tautomerization-Driven Mannich Reaction of Alkylazaarenes with Aminals.

We have developed a readily feasible Mannich-type reaction of alkylazaarenes with aminals, demonstrating excellent functional group tolerance and scalability. Mechanistic studies reveal that the reactivity and selectivity are governed by the electronic effects in conjunction with the stability and steric hindrance of the enamine intermediates. The resulting aminomethylated products hold promising potential for applications in organic synthesis, medicinal chemistry, and ligand design.

Achievement of pure transverse spin angular momentum at the focal plane by tightly focused generalized hybrid polarized vector beams.

The transverse spin angular momentum (SAM) observed in evanescent waves, interference fields, and tightly focused vector beams is directly related to both academic research and technical applications. In this work, we theoretically propose and experimentally generate the generalized hybrid polarized vector beam (GHPVB), which superimposes the orthogonal electric field vector components of the two cylindrical vector beams with topological charges and , with an initial phase difference of between them. First, we investigate the longitudinal SAM separation in weakly focused GHPVBs.

Enhancement of -carrying plasmid transfer in by hospital wastewater: a transcriptomic study.

Introduction: is a critical ESKAPE pathogen that presents a significant challenge to public health because of its multidrug-resistant strains. This study investigates the impact and mechanisms of hospital wastewater on the horizontal gene transfer of carbapenem resistance genes, particularly , in .

Methods: LB broth was prepared using sterile filtered wastewater as the substrate to investigate the impact of wastewater on the transfer of carbapenem-resistant gene in .

eCBT-I dialogue system: a comparative evaluation of large language models and adaptation strategies for insomnia treatment.

Background: Traditional face-to-face mental health treatments are often limited by time and space. Thanks to the development of advanced large language models (LLMs), digital mental health treatments can provide personalized advice to patients and improve compliance. However, in the field of CBT-I, specialized, real-time interactive dialogue platforms have not been fully developed.

A highly specific and ultrasensitive approach to detect Hylurgus ligniperda based on RPA-CRISPR-LbaCas12a-LFD system.

Background: Hylurgus ligniperda is an invasive bark beetle that poses a serious threat to global coniferous forests and the timber trade. Its broad host range, high reproductive potential, and strong environmental adaptability enable it to establish and spread rapidly in newly invaded regions. In October 2020, H.

Measurement properties of the Edmonton Frail Scale in older adults: A systematic review and meta-analysis.

Background: Frailty is a clinical condition characterised by heightened vulnerabilities to stressors and negative health consequences. The Edmonton Frail Scale is a prominent multidimensional tool for assessing frailty across various settings.

Objectives: This review aimed to synthesise and evaluate the certainty of evidence and the quality of Edmonton Frail Scale in older adults aged 60 and above with respect to its reliability (test-retest, inter-rater) and construct validity (convergent, known-group).

Identification and validation of DNA methylation-driven gene OSR1 as a novel tumor suppressor for the diagnosis and prognosis of breast cancer.

Introduction: Aberrant DNA methylation plays a critical role in the initiation and progression of cancer, yet its association with breast cancer remains inadequately defined. This study aims to clarify the link between methylation-driven genes and breast cancer pathogenesis.

Methods: RNA sequencing and DNA methylation data for breast cancer were retrieved from The Cancer Genome Atlas (TCGA).

Metabolic and survival responses to high temperature stress in the red-haired pine bark beetle Hylurgusligniperda.

Hylurgus ligniperda, a pine-infesting bark beetle, has successfully invaded Shandong, China, through imported forest timber, posing a significant threat to the country's forestry security. Heat tolerance is a critical survival strategy for insects to endure high summer temperatures, and understanding its mechanisms in H. ligniperda could improve pest forecasting and habitat suitability predictions.

Sperm metabolomic signatures of asthenozoospermia and teratozoospermia in Chinese reproductive-age men.

Asthenozoospermia and teratozoospermia are common causes of male infertility. Despite their prevalence, the underlying metabolic mechanisms remain poorly understood. In this study, we conducted targeted metabolomic profiling of sperm samples from 131 Chinese reproductive-age men (48 normozoospermic controls, 40 asthenozoospermic patients, and 43 teratozoospermic patients) to identify distinct metabolic signatures associated with these conditions.

Total Saponins of Leaves Alleviate Vascular Dementia via the Gut-Brain Axis.

Vascular dementia (VD) is the second most common type of dementia after Alzheimer's disease (AD), and chronic cerebral hypoperfusion (CCH) is the main causative factor of VD. Previous reports have confirmed that there is a relationship between VD and gut microbiota, and that some plant-based foods can improve VD through the gut-brain axis (GBA). leaves are a specialty food in Yunnan, China, and saponin is its main bioactive component.

Neurexin-3a IgG-mediated autoimmune encephalitis: a case report and literature review.

We summarized the clinical manifestations, auxiliary examinations, treatment, and prognostic characteristics of a patient with neurexin-3a IgG-mediated autoimmune encephalitis. On March 2, 2024, a 43-year-old male patient was admitted to the Second Hospital of Shandong University and had prodromal symptoms of infection before the onset of encephalitis. The main manifestations were episodic loss of consciousness, eyes turned upward to the right, clenched teeth, bleeding from tongue bite, and limb twitching.

Paralytic Shellfish Toxins in Coastal Waters of Changdao Island (China): Toxin Profiles, Potential Producers, and Environmental Conditions.

In recent decades, there have been frequent occurrences of paralytic shellfish toxin (PST) contamination in the Yellow and Bohai Seas, China. The waters around Changdao Island, situated at the convergence of these two seas, have suffered harmful algal blooms of spp., indicating a potential risk of PST contamination in shellfish.

Acupuncture effects of post-stroke thalamic pain: a systematic review and meta-analysis of randomized controlled trials.

Background: Post-stroke thalamic pain (PS-TP), a common form of central pain, is characterized by hyperalgesia and abnormal sensations in the contralateral affected area. Acupuncture treatment has shown increasing promise in treating PS-TP in recent years. This systematic review and meta-analysis aimed to evaluate the efficacy and safety of acupuncture treatment for PS-TP.

GPX4-dependent ferroptosis sensitivity is a fitness trade-off for cell enlargement.

Despite wide variation, each cell type has an optimal size. Maintaining optimal size is essential for cellular fitness and function but the biological basis for this remains elusive. Here, we performed fitness analysis involving genome-wide CRISPR-Cas9 knockout data from tens of human cell lines and identified that cell size influences the essentiality of genes related to mitochondria and membrane repair.

Healthcare professionals' perceptions of system preparedness during public health emergencies: a path analysis of mental health impacts.

Background: The easing of COVID-19 policies in China appears to have been inadequately prepared, leading to a profound shift in the mental wellbeing of healthcare professionals following the lifting of these measures. Our study aims to investigate the pathways underlying negative emotions experienced by healthcare professionals due to perceived inadequate system preparedness, aiming to enhance their mental health protection and facilitate more effective responses during future large-scale public health crises.

Methods: A total of 826 healthcare professionals were enrolled.

Head-to-Head Comparisons of the Distributional Characteristics and Measurement Properties of the 3-Level and 5-Level Versions of the EQ-5D-Y: A Systematic Review.

Objectives: This systematic review compared the distributional characteristics and measurement properties of EuroQol's EQ-5D-Y-3L and EQ-5D-Y-5L instruments, using results from published head-to-head comparative studies.

Methods: The review was reported following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses-COnsensus-based Standards for the selection of health Measurement INstruments (PRISMA-COSMIN) guideline. We searched 8 databases up to February 14, 2024 for studies on the measurement properties and distributional characteristics (missing data, ceiling/floor effects, consistency, and informativity) of both EQ-5D-Y versions.

The impact of behavioral activation on depressive symptoms in colorectal cancer patients within a medical environment: the mediating role of physical activity.

Colorectal cancer (CRC) treatment often affects patients' quality of life, leading to depressive symptoms. Behavioral activation (BA) therapy, which increases engagement by enhancing positive reinforcement and reducing avoidance, has shown potential in managing these symptoms. Physical activity (PA) is also known to alleviate depression, though its role as a mediator in BA's effectiveness remains unclear.

The role of glymphatic system function in the managing Cancer and Living Meaningfully (CALM) intervention to improve chemotherapy-related cognitive impairment (CRCI) in breast cancer survivors.

Objective: This study employed the diffusion tensor image analysis along the perivascular space (DTI-ALPS) index to investigate the glymphatic system's involvement in Managing Cancer and Living Meaningfully (CALM) intervention to improve chemotherapy-related cognitive impairment (CRCI) in breast cancer survivors (BCs).

Methods: 68 BCs were randomly assigned to the CALM (34 patients) or care-as-usual (CAU) (34 patients) groups. The Mini-Mental State Examination (MMSE) and Prospective and Retrospective Memory Questionnaire (PRMQ) were calculated for all patients before and after the intervention.

Injectable dual-cross-linked microalgae-silk gel ameliorates diabetic wound healing by promoting oxygenation and ROS clearance and lessening inflammation.

Hypoxia, excessive reactive oxygen species (ROS), and an impaired inflammatory microenvironment are key barriers to diabetic wound healing, collectively hindering cell migration, proliferation, and neovascularization, ultimately leading to failure in the healing process. Therefore, developing an effective therapeutic strategy capable of simultaneously addressing these challenges remains a critical clinical need. In this study, we developed CeS-Gel, an advanced hydrogel dressing integrating live microalgae and CeO₂ nanoparticles within a dual-crosslinked silk hydrogel network.

Targeting UAF1 Alleviate Neurotoxicity by Inhibiting APP/NLRP3 Axis-Mediated Pyroptosis and Apoptosis.

The accumulation of amyloid β (Aβ) protein, derived from the amyloid precursor protein (APP), plays a pivotal role in the pathogenesis of Alzheimer's disease (AD) by inducing neuronal cell injury. This study investigated the specific functions of ubiquitin-specific protease 1-associated factor 1 (UAF1) in mediating the neurotoxic effects triggered on Aβ. To model AD-related neuronal injury in vitro and in vitro, SH-SY5Y cells exposed to Aβ and APPswe/PS1dE9 (APP/PS1) transgenic mice were utilized.