Porcine respiratory disease complex induces pulmonary fibrosis related to the aberrant sphingolipid metabolism.

Porcine respiratory disease complex (PRDC) is a common syndrome in the modern swine industry worldwide, and its pathogenesis remains unclear to date. Our study aimed to investigate PRDC-induced pulmonary fibrosis and sphingolipid metabolism, and their relationship. Mouse and cell line (A549 and 3D4/21) models exposed to bleomycin and/or transforming growth factor-β1 (TGF-β1) were developed.

Replication of the GWAS-Identified GALNT13 rs10196189 Polymorphism in Relation to Speed-Power Elite Active Athlete Status and Multidimensional Phenotypic Differences in Chinese Han Males: A Pilot Study.

: Previous multi-ethnic genome-wide association studies (GWAS) have identified the GALNT13 rs10196189 polymorphism as a potential genetic marker linked to sprint-power performance. However, its relevance in East Asian populations, particularly the Han Chinese, remains untested. This study aimed to replicate the association of rs10196189 with elite sprint-power athlete status in Han Chinese males and examine its potential influence on physical performance traits and tissue-specific gene regulation.

GGTLC1 Is a Prognostic Biomarker in Renal Clear Cell Carcinoma.

Kidney renal clear cell carcinoma (KIRC), the most common type of renal cell carcinoma, is an immunobiologically and metabolically distinct tumor. Its clinical management is closely related to the tumor microenvironment (TME). Identifying its potential markers is important for improving prognosis.

Venlafaxine-induced thrombocytopenia: A case report and literature review.

Rationale: Drug-induced thrombocytopenia (DITP) is an adverse drug effect mediated by drug-dependent antibodies. Although several cases of thrombocytopenia induced by antidepressants and antianxiety drugs have been reported at home and abroad, reports of venlafaxine hydrochloride-induced thrombocytopenia are rare.

Patient Concerns: In this study, we report a case of an anxiety patient who developed severe thrombocytopenia and subcutaneous bleeding after 1 week of treatment with a venlafaxine-containing regimen.

Molecular Network Analysis and Effector Gene Prioritization of Endurance-Training-Influenced Modulation of Cardiac Aging.

Background/objectives: Cardiac aging involves the progressive structural and functional decline of the myocardium. Endurance training is a well-recognized non-pharmacological intervention that counteracts this decline, yet the molecular mechanisms driving exercise-induced cardiac rejuvenation remain inadequately elucidated. This study aimed to identify key effector genes and regulatory pathways by integrating human cardiac aging transcriptomic data with multi-omic exercise response datasets.

Application value of indocyanine green lymphography in the management of stage I/IIA microcystic lymphatic malformation of the tongue.

Background: This study aims to investigate whether indocyanine green lymphography (ICGL) facilitates the effectiveness and accuracy of current management of lingual microcystic lymphatic malformation (mLM).

Methods: This retrospective included patients under 18 years old with mLM of the tongue staged I or IIA, who underwent ICGL-aided surgical resection and sclerotherapy (ICGL group) or only surgical resection and sclerotherapy (non-ICGL group) between January 2019 and June 2024. The peri- and postoperative parameters of safety and feasibility were compared between two groups.

Integrating transcriptomic and metabolomic profiles of primiparous Holstein cows across multiple lactation periods reveals the regulatory mechanism underlying milk component traits.

To investigate the regulatory mechanisms underlying milk production traits, transcriptional sequencing (RNA-seq) and metabolomic analysis with liquid chromatography-MS were implemented in blood and serum samples from 25 lactating Holstein cows across multiple lactation stages. We identified 16,147, 15,859, and 16,010 expressed genes (fragments per kilobase million >1) and 160, 332, and 142 differentially expressed genes (DEG) during early, peak, and late lactation, respectively; the DEG were significantly enriched in adipocytokine, PPAR, and cAMP signaling and influenced cholesterol, vitamin B, and fat metabolism; 9 candidate genes were identified by comparing QTLs related to milk production, reproduction, and disease resistance traits. Additionally, 303 metabolites in 22 categories were detected, in which 19, 22, and 32 metabolites displayed differential abundance in the early versus peak, early versus late, and peak versus late comparison groups, respectively.

Dynamic Metabolic Changes Driven by Exercise-Intensity in Acute Swimming.

Purpose: Utilizing wearable devices and high-throughput omics technologies, the study aimed to establish an intensity-specific human acute swimming exercise model and draw a swimming metabolome atlas to clarify the commonalities and specificities in a panoramic dynamic physiological response under different intensities.

Methods: 42 healthy non-athlete young adults were randomly divided into moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) acute swimming groups. Blood samples were collected at baseline, 0, 15 and 30 minutes after swimming for metabolomics and targeted lipidomics testing.

Genetic and Functional Dissection of the NFKB2 Gene: Implications for Milk Fatty Acid Biosynthesis in Dairy Cattle.

Milk fat, an essential component of bovine milk, significantly impacts human health and dairy industry profitability. Our previous research identified that the NFKB2 gene was a key candidate for milk fatty acids (FAs) in dairy cattle. This study aimed to investigate the role of NFKB2 in regulating milk FAs in Holstein cattle through post-GWAS analysis.

[Retracted] Paeoniflorin inhibits nucleus pulposus cell apoptosis by regulating the expression of Bcl-2 family proteins and caspase-9 in a rabbit model of intervertebral disc degeneration.

[This retracts the article DOI: 10.3892/etm.2015.

Lipid profiling of Toxoplasma tachyzoites and the mouse host.

Our understanding of lipids in Toxoplasma gondii and its host is incomplete. This study aimed to analyze phospholipids and sphingolipids in tachyzoites and mice. RH strain tachyzoites were cultured and purified.

CCTα and GVI iPLA2-induced aberrant phosphatidylcholine metabolism contributes to pulmonary inflammation and fibrosis.

To date, no comprehensive profiling of phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) with pulmonary inflammation and fibrosis has been published. Our study aimed to analyze PC and LPC metabolism with the development and persistence of pulmonary inflammation and the progression to fibrosis; and their relationship. Mice and cell models exposed to bleomycin and/or transforming growth factor-β1 (TGF-β1) were developed; and porcine surrogates for pulmonary fibrosis were included.

Usefulness of intraoperative choledochoscopy in laparoscopic subtotal cholecystectomy for severe cholecystitis.

Laparoscopic subtotal cholecystectomy (LSC) has been a safe and viable alternative to conversion to laparotomy in cases of severe cholecystitis. The objective of this study is to determine the utility of intraoperative choledochoscopy in LSC for the exploration of the gallbladder, cyst duct, and subsequent stone clearance of the cystic duct in cases of severe cholecystitis. A total of 72 patients diagnosed with severe cholecystitis received choledochoscopy-assisted laparoscopic subtotal cholecystectomy (CALSC).

Structural and functional characterization of the brain-specific dynamin superfamily member RNF112.

Most members of the dynamin superfamily of large guanosine triphophatases (GTPases) have an ability to remodel membranes in response to guanosine triphosphate (GTP) hydrolysis. Ring Finger Protein 112 (RNF112) (ZNF179/neurolastin) is a recently identified brain-specific dynamin-like protein possessing a really interesting new gene (RING) finger domain. Despite its essential role as an E3 ligase in neuron development, the architecture of RNF112 and the exact role of its GTPase activity remain unknown.

Mitogenome of Uncaria rhynchophylla: genome structure, characterization, and phylogenetic relationships.

Background: Uncaria rhynchophylla is listed in the Chinese pharmacopoeia as one of the five botanical sources of the traditional medicine Gou-Teng, which has been utilized for the treatment of mental and cardiovascular disorders. This particular species is well-known in China for its hook-like structures originating from the leaf axils. Despite available reports on its chloroplast genome, there persists a notable lack of understanding concerning the structural variations and evolution of its mitochondrial genome.

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin.

The rigid-body rotation of the three-helical middle domain (3HB) relative to the GTPase domain of dynamin-like protein atlastin (ATL) is a crucial driver of homotypic membrane fusion within the endoplasmic reticulum (ER). Disruptions in this process have been associated with hereditary spastic paraplegia (HSP), a neurodegenerative disorder. Structural and biochemical studies suggest that the conformational changes in ATL are linked to GTP hydrolysis, but real-time visualization of these conformational dynamics during the GTP hydrolysis cycle remains challenging.

Circular RNA HIPK3 mediates epithelial-mesenchymal transition of retinal pigment epithelial cells by sponging multiple microRNAs.

Epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells plays key roles in the pathogenesis of multiple vitreoretinal diseases, leading to profound and permanent vision loss. Circular RNAs (circRNAs) are widespread and functional endogenous RNAs that could regulate gene expression in eukaryotes. The functions of circRNAs in mediating EMT has been reported in several diseases.

Pathological mechanisms and related markers of steroid-induced osteonecrosis of the femoral head.

Background: Osteonecrosis of the femoral head (ONFH) is a refractory orthopedic disease with a high disability rate. Long-term administration of steroids is the most common pathogenic factor for non-traumatic ONFH. Early diagnosis of steroid-induced osteonecrosis of the femoral head (SONFH) is difficult and mainly depends on imaging.

Synthesis, characterization and antifungal activity of imidazole chitosan derivatives.

Five novel imidazole-functionalized chitosan derivatives 3a-3e were synthesized via addition reactions of chitosan with imidazole derivatives. The partial incorporation of imidazole moiety in chitosan were confirmed by FTIR, UV, H NMR, XRD, SEM and GPC. Meanwhile, the antifungal activity against three common plant pathogenic fungi: Phytophthora nicotianae (P.

The effect of exercise training on heart rate variability in patients with hypertension: A systematic review and meta-analysis.

We aimed to assess the effect of exercise training on heart rate variability (HRV) in hypertensive patients and to provide practical recommendations. We systematically searched seven databases for randomized controlled trials (RCTs) comparing the efficacy of exercise interventions vs. non-exercise control for HRV in adults with hypertension.

Synthesis and Antifungal Activities of Glucosamine Aromatic Derivatives Against Four Phytopathogenic Fungi of Crops.

A series of diversified glucosamine derivatives (3a-3y) was synthesized and their antifungal activity was examined against four kinds of phytopathogens, Fusarium graminearum (F. graminearum), Fusarium moniliforme (F. moniliforme), Curvularia.

Strengthening and Toughening of ZG25SiMn2CrB Steel without Tempering Brittleness via Electropulsing Treatment.

High-strength low-alloy steels are widely used, but their traditional heat-treatment process is complex, energy-intensive, and makes it difficult to fully exploit the material's potential. In this paper, the electropulsing processing technology was applied to the quenching and tempering process of ZG25SiMn2CrB steel. Through microstructural characterization and mechanical property testing, the influence of electropulsing on the solid-state phase transition process of annealing steel was systematically studied.

Swimming Exercise Protocol and Care Methods for Pregnant Rats.

The developmental origins of health and disease concept highlights the impact of early environments on chronic non-communicable diseases like diabetes, cardiovascular disease, and cancer. Studies using animal models have investigated how maternal factors such as undernutrition, overnutrition, obesity, and exposure to chemicals or hypoxia affect fetal development and offspring health, leading to issues like low birth weight, high blood pressure, dyslipidemia, and insulin resistance. Given the increasing prevalence of overweight and obesity among reproductive-age women, effective interventions are critical.