Design and implementation of corrosion-resistant multitasking cell stainers.

Compared with machine staining, traditional manual staining faces various problems, such as a low preparation success rate, low efficiency, and harm to the human body due to corrosive gases. Therefore, a stainer that is of low cost, has strong corrosion resistance, and is suitable for small-batch preparation should be developed. In this study, by choosing a rotary scheme as the structural basis, a reusable container cover and a master-slave manipulator cooperation scheme are developed, which greatly improve the space utilization rate.

A lateral flow strip based on gold nanoparticles to detect 6-monoacetylmorphine in oral fluid.

We used lateral flow strips based on gold nanoparticles to detect 6-monoacetylmorphine (6-MAM; heroin's unique metabolite) in oral fluid samples. In this competitive lateral chromatographic immunoassay, the 6-MAM was chemically synthesized and conjugated to bovine serum albumin. The results were qualitatively detected via the colour change of the test line.

A novel multi-walled carbon nanotube-based antibody conjugate for quantitative and semi-quantitative lateral flow assays.

In this study, the multi-walled carbon nanotubes (MWCNTs) were applied in lateral flow strips (LFS) for semi-quantitative and quantitative assays. Firstly, the solubility of MWCNTs was improved using various surfactants to enhance their biocompatibility for practical application. The dispersed MWCNTs were conjugated with the methamphetamine (MET) antibody in a non-covalent manner and then manufactured into the LFS for the quantitative detection of MET.

[The Study on Combined Screening Method for TBS and Cervical Cancer Cell’s DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology].

The traditional screening method of cervical cancer mainly involves TBS classification method and quantitative analysis method based on DNA, TBS screening method which has high diagnostic rate, but it needs experienced doctors to participate in the process with low sensitivity. Therefore, it is difficult to achieve early cervical cancer screening; cell’s DNA quantitative analysis, only stained the nucleus, achieving a quantitative and automated analysis. Even with high sensitivity, the specificity is poor.

[Study of Cervical Exfoliated Cell's DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology].

The conventional cervical cancer screening methods mainly include TBS (the bethesda system) classification method and cellular DNA quantitative analysis, however, by using multiple staining method in one cell slide, which is staining the cytoplasm with Papanicolaou reagent and the nucleus with Feulgen reagent, the study of achieving both two methods in the cervical cancer screening at the same time is still blank. Because the difficulty of this multiple staining method is that the absorbance of the non-DNA material may interfere with the absorbance of DNA, so that this paper has set up a multi-spectral imaging system, and established an absorbance unmixing model by using multiple linear regression method based on absorbance's linear superposition character, and successfully stripped out the absorbance of DNA to run the DNA quantitative analysis, and achieved the perfect combination of those two kinds of conventional screening method. Through a series of experiment we have proved that between the absorbance of DNA which is calculated by the absorbance unmixxing model and the absorbance of DNA which is measured there is no significant difference in statistics when the test level is 1%, also the result of actual application has shown that there is no intersection between the confidence interval of the DNA index of the tetraploid cells which are screened by using this paper's analysis method when the confidence level is 99% and the DNA index's judging interval of cancer cells, so that the accuracy and feasibility of the quantitative DNA analysis with multiple staining method expounded by this paper have been verified, therefore this analytical method has a broad application prospect and considerable market potential in early diagnosis of cervical cancer and other cancers.

In vitro selection of DNA aptamers binding pesticide fluoroacetamide.

Fluoroacetamide (Mw = 77.06) is a lethal rodenticide to humans and animals which is still frequently abused in food storage somewhere in China. The production of antibodies for fluoroacetamide is difficult due to its high toxicity to animals, which limits the application of immunoassay method in poison detection.

[Biological Process Oriented Online Fourier Transform Infrared Spectrometer].

An online Fourier Transform Infrared Spectrometer and an ATR (Attenuated Total Reflection) probe, specifically at the application of real time measurement of the reaction substrate concentration in biological processes, were designed. (1) The spectrometer combined the theories of double cube-corner reflectors and flat mirror, which created a kind of high performance interferometer system. The light path folding way was utilized to makes the interferometer compact structure.

[Moving Mirror Scanning System Based on the Flexible Hinge Support].

In order to improve moving mirror drive of Fourier transform infrared spectrometer, we design a dynamic scanning system based on flexible hinge support. Using the flexible hinge support way and the voice coil motor drive mode. Specifically, Using right Angle with high accuracy high stability type flexible hinge support mechanism support moving mirror, dynamic mirror can be moved forward and backward driven by voice coil motor reciprocating motion, DSP control system to control the moving mirror at a constant speed.

[Hadamard Transform NIR Spectrometer for the Biological Processes].

Monitoring the consumption of nutrients of biological process helps control the growth environment of the microbes. It ensures the microbes are always in the best growing conditions, so as to maximize the yield of the target product. To monitor the content of glycerol, methanol and glucose in yeast culture medium, a new NIR spectrometer was developed which is based on Hadamard Transform (HT)technique.

[AOTF-Based Imaging Spectrometer and Its Applications in the Identification of Metameric Targets].

For obtaining spectrum information and space distribution information of metameric targets at the same time, an AOTF-based imaging spectrometer was developed, which consists of a front imaging lens assembly, an AOTF filter imaging module, an AOTF driver, a plane array CCD, an image acquisition card and a PC. Under the control of the PC, a spectral image cube can be obtained by a fast wavelength scanning process, thereby the spectrum information of any point on the image is available. The developed imaging spectrometer, with a spectral range from 550-1,000 nm and a spectral resolution of 2.

Rapid analysis of diesel fuel properties by near infrared reflectance spectra.

In this study, based on near infrared reflectance spectra (NIRS) of 441 samples from four diesel groups (-10# diesel, -20# diesel, -35# diesel, and inferior diesel), three spectral analysis models were established by using partial least square (PLS) regression for the six diesel properties (i.e., boiling point, cetane number, density, freezing temperature, total aromatics, and viscosity) respectively.

Determination of co-administrated opioids and benzodiazepines in urine using column-switching solid-phase extraction and liquid chromatography-tandem mass spectrometry.

Co-administration of opioids with benzodiazepines is very common around the world. A semi-automated method was developed for the determination of four opioids and two benzodiazepines as well as their metabolites (including glucuronide metabolites) in human urine, based on on-line column-switching-solid-phase extraction (CS-SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The CS-SPE was performed by loading 200μL of urine sample to an Oasis HLB cartridge.

[DNA aptamer selection in vitro for determining ketamine by FluMag-SELEX].

Objective: To select specific DNA aptamer for determining ketamine by FluMag-SELEX.

Methods: Based on magnetic beads with tosyl surface modification as solid carrier and ketamine as target, a random ssDNA library with total length of 78 bp in vitro was compounded. After 13 rounds screening, DNA cloning and sequencing were done.

[Development of a portable mid-infrared rapid analyzer for oil concentration in water based on MEMS linear sensor array].

Aiming at the existing problems such as weak environmental adaptability, low analytic efficiency and poor measuring repeatability in the traditional spectral oil analyzers, the present paper designed a portable mid-infrared rapid analyzer for oil concentration in water. To reduce the volume of the instrument, the non-symmetrical folding M-type Czerny-Turner optical structure was adopted in the core optical path. With a periodically rotating chopper, controlled by digital PID algorithm, applied for infrared light modulation, the modulating accuracy reached ±0.

Screening of drugs of abuse and toxic compounds in human whole blood using online solid-phase extraction and high-performance liquid chromatography with time-of-flight mass spectrometry.

A novel method for the screening of 151 drugs of abuse and toxic compounds in human whole blood has been developed and validated by online solid-phase extraction with liquid chromatography coupled to time-of-flight mass spectrometry. Analytes were extracted and separated by using a fully automated online solid-phase extraction liquid chromatography system with total chromatographic run time of 26 min. Time-of-flight mass spectrometry screening of 151 drugs of abuse and toxic compounds was performed in a full-scan (m/z 50-800) mode using an MS(E) acquisition of molecular ions and fragment ions data at two collision energies (one was 6 eV and another one was in the range of 5-45 eV).

Determination of ecgonine and seven other cocaine metabolites in human urine and whole blood by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry.

Ecgonine is suggested to be a promising marker of cocaine (COC) ingestion. A combined mass spectrometry (MS) and tandem MS (MS/MS) method was developed to simultaneously determine ecgonine and seven other metabolites of cocaine in human urine and whole blood with ultra-high-pressure liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The compounds were extracted from as little as 100 μL of sample by solid-phase extraction with a 96-well μElution solid-phase extraction plate.

[Design of a compact structure interferometer].

A novel interferometer system based on the combinations of cube-corner reflectors and fixed plane mirrors was designed, the moving mirror drive system was designed and analysed, and its governor PID algorithm was used to ensure that the movement of the moving mirror is collimated, uniform and smooth. The parameters of the optical system of the interferometer and the optical devices were described. Finally, after validation of the experiment, it was indicated that the wave number accuracy, resolution, signal to noise ratio and other key indicators can meet the needs of practical application.

[Design of a high-throughput and wide-bandwidth near-infrared acousto-optic tunable filter].

The acousto-optic tunable filter (AOTF) is the most outstanding progress of near-infrared spectrometer since the 1990s. Restriction in working range and performance of the device exists due to the traditional single-transducer structure. The design of a near-infrared acousto-optic tunable filter with high-throughput and wide-bandwidth adopting a dual-transducer structure is reported in the present paper.

[Testing method research for key performance indicator of imaging acousto-optic tunable filter (AOTF)].

Imaging AOTF is an important optical filter component for new spectral imaging instruments developed in recent years. The principle of imaging AOTF component was demonstrated, and a set of testing methods for some key performances were studied, such as diffraction efficiency, wavelength shift with temperature, homogeneity in space for diffraction efficiency, imaging shift, etc.

[A two-dimensional double dispersed hadamard transform spectrometer].

A kind of two-dimensional hadamard transform spectrometer was developed. A grating was used for chromatic dispersion of orders and a prism was used for spectral dispersion. Quite different from traditional CCD detection method, a digital micromirror device (DMD) was applied for optical modulation, and a simple point detector was used as the sensor.

[An interferometer design for Fourier transform infrared spectrometer].

A novel interferometer system based on the combinations of cube-corner reflectors and fixed plane mirrors was designed to improve the quality of spectra. It consists of a pair of cube corner reflectors, two fixed perpendicular plane mirrors and one beam splitter. By analyzing the modulation depth and phase error, the tilt and shearing of the cube corner reflectors, the deviation angle of cube corner reflector, the tilt of fixed plane mirrors and the deviation angle of two fixed plane mirrors were analyzed.

[A digital micromirror device-based Hadamard transform near infrared spectrometer].

A Hadamard transform near infrared spectrometer based on a digital micromirror device was constructed. The optical signal was collected by optical fiber, a grating was used for light diffraction, a digital micromirror device (DMD) was applied instead of traditional mechanical Hadamard masks for optical modulation, and an InGaAs near infrared detector was used as the optic sensor. The original spectrum was recovered by fast Hadamard transform algrithms.

[Observation of and research on intravital microcirculation use of orthogonal polarization multi-spectral technique].

A small, simple intravital microcirculation multi-spectral imaging system was constructed. Orthogonal polarization spectral (OPS) imaging and liquid crystal tunable filter (LCTF) were used for intravital microcirculation multi-spectral imaging. LCTF has excellent imaging quality and low-power loss, without moving parts and image shift.

Quantum-dot-based immunofluorescent imaging of HER2 and ER provides new insights into breast cancer heterogeneity.

Breast cancer (BC) is a heterogeneous tumor, and better understanding of its heterogeneity is essential to improving treatment effect. Quantum dot (QD)-based immunofluorescent nanotechnology (QD-IHC) for molecular pathology has potential advantages in delineating tumor heterogeneity. This potential is explored in this paper by QD-IHC imaging of HER2 and ER.

[Development of a new FTIR car-borne oil content analyzer].

Turlun structure was introduced into the Fourier transform interference system in the present paper in order to decrease the size and cost of the whole system and to increase the ability of vibration resistance to meet the requirement of car-borne outdoor monitoring application. The speed deviation of the voice coil motor (VCM) driving system, which is based on feedback of interference wavelength of reference laser and DSP control, is within 5%. The standard oil sample was used to test the performance of the spectrometer.