Transcriptomic and Metabolomic Signatures of Acute Circadian Misalignment in Mice.

Acute circadian misalignment, such as that induced by a single episode of jet lag, can leave molecular traces even after behavioral rhythms appear to recover. Here, we applied an integrated multi-omics approach-combining liver transcriptomics and plasma metabolomics-to characterize residual signatures on the 7th day after a single 6-h phase advance in male mice. Our data revealed significant alterations, particularly in the core clock genes Bmal1 and Cry1, and the metabolites l-arginine and SM(d18:1/18:1(11Z)), with notable differences at Zeitgeber Time 0 (ZT0).

Association of early hyperglycemia with morbidity and mortality in very low birth weight infants.

Objective: To evaluate the association between hyperglycemia and morbidity/mortality in very-low-birth-weight infants.

Methods: This retrospective study analyzed 255 very-low-birth-weight infants admitted to a Neonatal Intensive Care Unit (2020-2024). Infants were stratified by glucose levels: hyperglycemia (> 150 mg/dL) vs.

Screening of salt-tolerant soybean germplasm and study of salt-tolerance mechanism.

Screening of salt-tolerant soybean varieties, identification of key genes and verification of gene function through salt-tolerance assessment, transcriptome profiling and soybean hairy root transformation experiments. Screening and breeding of salt-tolerant soybean varieties is essential to increase the area and yield of soybean on saline-alkaline soils. In this study, 81 soybean varieties were systematically assessed for salt tolerance during both germination and early seedling development stages.

Qualitative and quantitative pesticide residue analysis in Allium tuberosum using an electronic nose with multivariate analysis.

As the pesticide residue issue in Allium tuberosum becomes more severe, developing a rapid and robust detection method to assess its pesticide residue concentration is crucial. This study proposes a combined method based on the electronic nose (E-nose), Particle Swarm Optimization (PSA) algorithm, and Support Vector Machine (SVM) for both qualitative and quantitative detection of pesticide residues in Allium tuberosum. The results show that the SVM classifier effectively distinguishes between normal and contaminated samples, with a classification accuracy of 100 %.

Enteric Delivery of Probiotics: Challenges, Techniques, and Activity Assays.

Probiotics, as live microbial agents, play a pivotal role in modulating host microbiota balance, enhancing immunity, and improving gastrointestinal health. However, their application is hindered by critical challenges, such as inactivation during processing, storage, and gastrointestinal delivery, as well as low colonization efficiency. This article comprehensively reviews recent advances in probiotic delivery systems, focusing on innovative technologies, including hydrogels, nanocoatings, emulsions, and core-shell microgels.

Development and validation of a BMI stratified mortality prediction model for patients with COPD complicated by HF using the MIMIC-IV database.

Unlabelled: Given the high mortality rate of chronic obstructive pulmonary disease (COPD) complicated by heart failure (HF), early identification of high-risk patients and timely intervention are crucial. There is currently no in-hospital mortality risk prediction model for COPD complicated by HF patients with different Body Mass Index (BMI). This study aims to explore the risk factors of COPD complicated by HF and construct an in-hospital mortality risk prediction model.

Multi-task detection of pesticide residues in Allium tuberosum using a colorimetric sensor array based on anthocyanin dyes combined with a pattern recognition algorithm.

Rapid and reliable detection of pesticide residues in Allium tuberosum is essential to ensure food safety and sustainable agriculture. In this study, a novel colorimetric sensor array (CSA) was developed using nine environmentally friendly anthocyanins as dyes and silica gel plates as carriers. And the color change of the CSA in this study was verified to relate to the potential-of-Hydrogen of volatile organic compounds in the sample by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry.

A Review on Improving the Oxidative Stability of Pine Nut Oil in Extraction, Storage, and Encapsulation.

Pine nut oil (PNO) is highly valued by consumers for its rich content of unsaturated fatty acids, which confer unique nutritional benefits. However, PNO is highly susceptible to lipid oxidation during storage and extraction. This chemical degradation compromises product quality and poses potential risks to food safety.

Online 4D Ultrasound-Guided Robotic Tracking Enables 3D Ultrasound Localisation Microscopy with Large Tissue Displacements.

Super-Resolution Ultrasound (SRUS) imaging through localising and tracking microbubbles, also known as Ultrasound Localisation Microscopy (ULM), has demonstrated significant potential for reconstructing microvasculature and flows with sub-diffraction resolution in clinical diagnostics. However, imaging organs with large tissue movements, such as those caused by respiration, presents substantial challenges. Existing methods often require breath holding to maintain accumulation accuracy, which limits data acquisition time and ULM image saturation.

Optic Atrophy 1: The Conductor of Cellular Harmony and Age-Related Pathologies.

As the population aging, the prevalence of age-related diseases is also rising. Mitochondrial malfunction is one of the hallmarks of aging, and optic atrophy type 1 (OPA1), a protein found in the inner membrane (IM) of mitochondrial, is essential to this process. OPA1 regulates the fusion of IM and cristae structure, hence maintaining cellular energy metabolism and function.

Disease burden and attributable risk factors of major depressive disorder in China, Japan, and South Korea from 1990 to 2021 and its prediction to 2035.

Objective: To analyze the disease burden of major depressive disorder (MDD) and risk factors associated with MDD in China, Japan, and South Korea(CJK) from 1990 to 2021, to explore the effects of age, period, and cohort on the disease burden of MDD, and to predict the burden of MDD in CJK from 2021 to 2035.

Methods: The Global Burden of Disease 2021 (GBD) database was searched to collect the incidence, prevalence, disability-adjusted life years (DALYs), and risk factors of MDD in CJK. The respondents were selected from the Global, China, Japan, and South Korea.

The Importin-β Protein LbSAD2 Enhances Salt Gland Development and Salt Resistance in the Recretohalophyte Limonium bicolor.

Limonium bicolor is a typical recretohalophyte with specialised salt glands that secrete excessive Na out of the plant. The detailed mechanisms of salt gland development and salt resistance are largely unclear. Here, we investigated the function of the importin-β protein LbSAD2 from L.

Colony pattern multistability emerges from a bistable switch.

Microbial colony development hinges upon a myriad of factors, including mechanical, biochemical, and environmental niches, which collectively shape spatial patterns governed by intricate gene regulatory networks. The inherent complexity of this phenomenon necessitates innovative approaches to comprehend and compare the mechanisms driving pattern formation. Here, we unveil the multistability of bacterial colony patterns, where bacterial colony patterns can stabilize into multiple distinct types including ring-like patterns and sector-like patterns on hard agar, orchestrated by a simple synthetic bistable switch.

LbMYB368 from the recretohalophyte Limonium bicolor promotes salt gland development and salinity tolerance.

Halophytes can grow and reproduce normally in an environment containing more than 200 mM NaCl, offering untapped gene resources for improving crop salinity tolerance. As a recretohalophyte, Limonium bicolor can secrete excess Na through salt glands, specialized structures on the leaf and stem epidermis. Here, we identified a MYB transcription factor gene, LbMYB368, that is highly expressed during salt gland development.

Circadian control of hepatic ischemia/reperfusion injury via HSD17B13-mediated autophagy in hepatocytes.

Background & Aims: Studies have illustrated the role of circadian rhythms in hepatic ischemia/reperfusion injury (HIRI), but the mechanisms are poorly understood. Bmal1 plays a significant role in the circadian control of liver physiology and disease; however, its role in HIRI has not been investigated. Here, we aimed to explore the potential contribution of BMAL1 to HIRI.

NAC transcription factor PpNAP4 positively regulates the synthesis of carotenoid and abscisic acid (ABA) during peach ripening.

Yellow-fleshed peaches (Prunus persica (L.) Batsch) are recognized as an excellent dietary source of carotenoids. The metabolic process of carotenoids in plants has been extensively characterized; however, the molecular mechanisms controlling carotenoid accumulation in peaches, particularly the transcriptional regulators upstream this process, remain poorly understood.

Reprogramming peritoneal macrophages with outer membrane vesicle-coated PLGA nanoparticles for endometriosis prevention.

Endometriosis is a chronic inflammatory disease that primarily affects women of reproductive age. The current hormonal treatments are unsuitable for women who wish to conceive, highlighting the need for non-hormonal therapeutic alternatives. In this study, we engineered outer membrane vesicle (OMV)-coated poly (lactic-co-glycolic acid) (PLGA) nanoparticles (OMV-NPs) as a potential therapy for endometriosis.

Evaluation and categorization of various pea cultivars utilizing near-infrared spectroscopy in conjunction with multivariate statistical techniques.

The swift detection of allergenic protein and other nutritional indicators in pea protein is crucial for food and breeding efforts, facilitating the targeted selection of specific pea varieties and the advancement and processing of healthful foods. Using near-infrared (NIR) spectroscopy, spectral data for different pea varieties in the range of 908-1676 nm were collected, which were subsequently integrated with chemical values obtained by conventional methods. Multivariate statistical analysis was employed to optimize, develop, and validate the model for the spectral data.

Mild liver injury following withdrawal of long-term prednisone therapy: A case report.

Background: Liver injury manifesting as hepatic enzyme abnormalities, has been occasionally identified to be a feature of primary or secondary Addison's disease, an uncommon endocrine disease characterized by adrenal insufficiency. There have been no more than 30 reported cases of liver injury explicitly attributed to Addison's disease. Liver injury resulting from adrenal insufficiency due to glucocorticoid withdrawal is exceptionally rarer.

The trends of lung cancer burden in BRICS from 1990 to 2021 and its projection to 2035.

Background: Lung cancer has become the malignant tumor with the highest morbidity and mortality in the world. This study aims to analyze the burden of lung cancer and risk factors associated with lung cancer in the BRICS from 1990-2021 and to project the burden of lung cancer in the BRICS from 2021-2035.

Methods: The Global Burden of Disease (GBD) 2021 database was searched to collect the incidence, prevalence, mortality, disability-adjusted life years (DALYs), and risk factors of lung cancer in the BRICS.

High-throughput near-infrared spectroscopy for detection of major components and quality grading of peas.

Pea ( L.) is a nutrient-dense legume whose nutritional indicators influence its functional qualities. Traditional methods to identify these components and examine the relationships between their contents could be more laborious, hindering the quality assessment of the varieties of peas.

PpNAP4 and ethylene act in a regulatory loop to modulate peach fruit ripening and softening.

Ripening significantly influences fruit quality and commercial value. Peaches (Prunus persica), a climacteric fruit, exhibit increased ethylene biosynthesis and decreased fruit firmness during ripening. NAC-like proteins activated by AP3/P1 (NAP) proteins are a subfamily of NAC transcription factors, and certain NAPs have been shown to intervene in fruit ripening.

PpMYB10.1 regulates peach fruit starch degradation by activating PpBAM2.

Starch degradation, a crucial source for soluble sugar, significantly influences fruit flavor development during ripening. Key enzymes in this process include α-amylases (AMYs) and β-amylases (BAMs). In this study, we identified 5 PpAMYs and 9 PpBAMs in peach and categorized them into three and four groups, respectively, based on the gene structures and the phylogenetic analysis.

The role of ATP-binding Cassette subfamily B member 6 in the inner ear.

ABCB6 has been implicated in dyschromatosis universalis hereditaria, a condition characterized by hyperpigmented and hypopigmented skin macules. Dyschromatosis universalis hereditaria can also present with hearing loss. Dyschromatosis universalis hereditaria-associated mutations in ABCB6 have been reported, but the role of this protein in the inner ear has not been studied.