Molecular characterization of Spodoptera frugiperda nose resistant to fluoxetine protein 6 and its putative involvement in tolerance to cyantraniliprole.

Spodoptera frugiperda (FAW) is a notorious polyphagous pest that has developed resistance to various insecticides including diamide insecticides. Our previous study established a FAW cyantraniliprole-resistant (SfCYAN-R) strain by laboratory resistance selection of susceptible strain (SfCYAN-S), however, the potential resistance mechanisms of FAW to cyantraniliprole remain unclear. In this study, SfNrf6 encoding nose resistant to fluoxetine (Nrf) protein 6 was identified to be upregulated in SfCYAN-R strain compared with SfCYAN-S strain based on RNA-Seq data and RT-qPCR.

Effect of membrane rigidification on the expression and cold-tolerance in .

Introduction: The cold tolerance of winter rapeseed cultivars is critically important for winter survival and yield formation in northern area. BrAFP1, an antifreeze protein in , is hypothesized to stabilize membranes and inhibit ice crystal formation.

Methods: we cloned the promoter from the cold-tolerant cultivar Longyou 7 (L7) and constructed the expression vector to investigate the impact of membrane state changes on BrAFP1 expression and the cold tolerance in winter rapeseed.

Impact of robotic-assisted versus video-assisted thoracoscopic surgery on efficacy and quality of life in patients with pulmonary opacities: a propensity score-matched analysis.

Objective: With the increasing detection of pulmonary opacities through low-dose computed tomography (LDCT), minimally invasive surgical techniques have gained prominence. While debates persist regarding the comparative efficacy and postoperative quality of life (QOL) between robot-assisted thoracoscopic surgery (RATS) and video-assisted thoracoscopic surgery (VATS), this study aimed to compare the perioperative outcomes and postoperative QOL between RATS and VATS in patients with pulmonary opacities.

Methods: A retrospective analysis was performed on patients who underwent pulmonary opacities resection at the Department of Thoracic Surgery, Guangxi Zhuang Autonomous Region People's Hospital, between January 2021 and November 2024.

Characterizations and transcriptional response of peroxiredoxin genes, TcPrx6a and TcPrx6b, to paraquat and deltamethrin exposures in Tribolium castaneum.

Prx6 attracts particular attention because of its ability to reduce the widest range of peroxidative substrates among the peroxiredoxin (Prx) family. In this study, TcPrx6a and TcPrx6b were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcPrx6a and TcPrx6b were highly expressed in fat body and head, respectively, and both were upregulated after exposure of the beetles to paraquat and deltamethrin.

Genome-wide association studies and transcriptome analysis reveal novel genes associated with freezing tolerance in rapeseed (Brassica napus L.).

Freezing stress is the main obstacle affecting the geographical distribution, growth, development, quality, and productivity of rapeseed (Brassica napus) in northern China. However, there is a little knowledge of rapeseed freezing tolerance mechanism. Here, 289 core germplasms collected from 36 countries were used to identify SNPs associated with freezing tolerance.

The Elongator complex regulates larval-pupal metamorphosis by modulating ecdysteroid biosynthesis in the red flour beetle, Tribolium castaneum.

The highly conserved Elongator complex plays important roles in histone acetylation and tRNA modification. Currently, Elongator complex has been shown to be essential for a range of biological processes, but its function in insect hormone signaling is poorly understood. In this study, the cDNA encoding TcElp3, the catalytic subunit of the Elongator complex in Tribolium castaneum, was cloned and functionally characterized.

Elongator subunit Elp3 regulates reproduction in Tribolium castaneum by interacting with FOXO.

As the catalytic subunit of the Elongator complex, Elongator protein 3 (Elp3) plays a crucial role in multiple physiological processes, including growth, development and immune responses. Previous studies on Elp3 have focused on Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens (human) or Mus musculus (mouse), whereas there are few reports on Elp3 in agricultural pests. Here, the role of TcElp3 in reproduction in the red flour beetle, Tribolium castaneum, was investigated, and the underlying mechanisms were explored.

BnaHSFA2, a heat shock transcription factor interacting with HSP70 and MPK11, enhances freezing tolerance in transgenic rapeseed.

Heat shock transcription factors (Hsfs) play important roles in plant developmental regulations and various abiotic stress responses. However, their evolutionary mechanism of freezing tolerance remains poorly understood. In our previous transcriptomics study based on DNA methylation sequencing, the BnaHsfA2 was found to be significantly accumulated in winter rapeseed (Brassica rapa L.

Genome-Wide Identification of Freezing-Responsive Genes in a Rapeseed Line NTS57 Tolerant to Low-Temperature.

Winter rapeseed is a high-oil crop that exhibits significant sensitivity to low temperatures, leading to a substantial reduction in production. Hence, it is of great significance to elucidate the genomic genetic mechanism of strong freezing-resistant winter rapeseed to improve their freezing-resistant traits. In this study, global transcriptome expression profiles of the freezing-resistant cultivar NTS57 (NS) under freezing stress were obtained for the years 2015, 2016, and 2017 by RNA sequencing (RNA-seq).

Co-application of Validamycin A and dsRNAs targeting trehalase genes conferred enhanced insecticidal activity against Laodelphax striatellus.

The small brown planthopper (SBPH), Laodelphax striatellus, poses a significant threat to rice crops, necessitating innovative pest control strategies. This study evaluated the potential of validamycin A (Val A) and RNA interference (RNAi) targeting trehalase genes (LsTre1 and LsTre2) in controlling SBPH. Our results demonstrated that Val A treatment of rice seedlings led to a dose-dependent mortality of SBPH.

Risk assessment, fitness cost and transcriptome analysis of cyantraniliprole resistance in Spodoptera frugiperda.

Spodoptera frugiperda is a notorious invasive pest causing substantial yield losses of crops and has developed resistance to various types of insecticides. In this study, a cyantraniliprole-resistant strain, SfCYAN-R, was obtained from a susceptible strain, SfCYAN-S, after 13 generations of selection with cyantraniliprole. The fitness cost in SfCYAN-R strain was evaluated, and the putative resistance-related genes were explored by RNA-seq analysis.

The Truncated Peptide AtPEP1 Has the Same Function as AtPEP1 in Inhibiting Primary Root Growth and Triggering of ROS Burst.

Currently, the widely used active form of plant elicitor peptide 1 (PEP1) from is composed of 23 amino acids, hereafter AtPEP1, serving as an immune elicitor. The relatively less conserved N-terminal region in AtPEP family indicates that the amino acids in this region may be unrelated to the function and activity of AtPEP peptides. Consequently, we conducted an investigation to determine the necessity of the nonconserved amino acids in AtPEP1 peptide for its functional properties.

Functional Characterization and Putative Regulatory Mechanism of an RNAi Efficiency-Related Nuclease (REase) in the Fall Armyworm, .

The lepidopteran-specific RNAi efficiency-related nuclease (REase) has been shown to contribute to double-strand RNA (dsRNA) degradation in several lepidopteran insects. However, little is known about its regulatory mechanism. In this study, we identified and characterized in .

Involvement of Glutamate Cysteine Ligase Genes in Tolerance to Emamectin Benzoate in and Their Putative Regulatory Mechanisms.

As the rate-limiting enzyme in Glutathione (GSH) biosynthesis, the mammalian glutamate cysteine ligase (Gcl) catalytic (Gclc) and modifier (Gclm) subunits are regulated at multiple levels, whereas the function and regulatory mechanism of insect Gcl remain to be explored. In this study, we identified and characterized and in . and were highly expressed in the hindgut and relatively less expressed in other tissues.

The BrAFP1 promoter drives gene-specific expression in leaves and stems of winter rapeseed (Brassica rapa L.) under cold induction.

BrAFP1(antifreeze protein in winter turnip rape) effectively limits recrystallization and growth of ice crystals. The BrAFP1 expression level determines whether the freezing-induced damage to winter turnip rape plants is avoided. This study analyzed the activity of the BrAFP1 promoters of several varieties at various cold tolerance levels.

The pleiotropic AMPK-CncC signaling pathway regulates the trade-off between detoxification and reproduction.

The association of decreased fecundity with insecticide resistance and the negative sublethal effects of insecticides on insect reproduction indicates the typical trade-off between two highly energy-demanding processes, detoxification and reproduction. However, the underlying mechanisms are poorly understood. The energy sensor adenosine monophosphate-activated protein kinase (AMPK) and the transcription factor Cap "n" collar isoform C (CncC) are important regulators of energy metabolism and xenobiotic response, respectively.

Comparative phosphoproteomics analysis provides insights into the responses of Chilo suppressalis to sublethal chlorantraniliprole exposure.

Background: Sublethal exposure to insecticides causes changes in insect behaviors and physiologies including feeding, mobility, communication, hormone homeostasis, development and fecundity, however, the underlying molecular mechanisms were largely unclear. Our previous studies revealed that sublethal chlorantraniliprole exposure disturbed the hormone homeostasis, reduced the weight and longevity and prolonged the developmental duration of Chilo suppressalis. In the present study, the potential phosphorylation modification regulation mechanisms in C.

Rice lipid transfer protein, OsLTPL23, controls seed germination by regulating starch-sugar conversion and ABA homeostasis.

Seed germination is vital for ensuring the continuity of life in spermatophyte. High-quality seed germination usually represents good seedling establishment and plant production. Here, we identified OsLTPL23, a putative rice non-specific lipid transport protein, as an important regulator responsible for seed germination.

Molecular Characterization of Heme Oxygenase and Its Involvement in Susceptibility to Chlorantraniliprole.

The mammalian heme oxygenase (HO) plays an important role in cytoprotection against oxidative-stress-induced cell damage; however, functional characterization of insect HO is still limited. In this study, cDNA encoding a HO, named , was cloned from . Analysis of the transcription level and enzymatic activity showed that exposure of the LC concentration of chlorantraniliprole to the third instar larvae significantly upregulated both the mRNA level and enzymatic activity of at 24 h after treatment.

DNA methylation affects freezing tolerance in winter rapeseed by mediating the expression of genes related to JA and CK pathways.

Winter rapeseed is the largest source of edible oil in China and is especially sensitive to low temperature, which causes tremendous agricultural yield reduction and economic losses. It is still unclear how DNA methylation regulates the formation of freezing tolerance in winter rapeseed under freezing stress. Therefore, in this study, the whole-genome DNA methylation map and transcriptome expression profiles of freezing-resistant cultivar NTS57 (NS) under freezing stress were obtained.

Integrated methylome and transcriptome analysis unravel the cold tolerance mechanism in winter rapeseed(Brassica napus L.).

Background: Cytosine methylation, the main type of DNA methylation, regulates gene expression in plant response to environmental stress. The winter rapeseed has high economic and ecological value in China's Northwest, but the DNA methylation pattern of winter rapeseed during freezing stress remains unclear.

Result: This study integrated the methylome and transcriptome to explore the genome-scale DNA methylation pattern and its regulated pathway of winter rapeseed, using freezing-sensitive (NF) and freezing-resistant (NS) cultivars.

Genome-Wide Characterization and Expression Analysis of GeBP Family Genes in Soybean.

The glabrous-enhancer-binding protein (GeBP) family is a family of plant-specific transcription factors, whose members share a central DNA-binding domain. Previous studies have already proven that genes are involved in the control of cell expansion but not cell proliferation in . However, there has not yet been a versatile analysis of the genes' function in soybean ( L.

Integrate QTL Mapping and Transcription Profiles Reveal Candidate Genes Regulating Flowering Time in .

Flowering at the proper time is an important part of acclimation to the ambient environment and season and maximizes the plant yield. To reveal the genetic architecture and molecular regulation of flowering time in oilseed rape (), we performed an RNA-seq analysis of the two parents after vernalization at low temperature and combined this with quantitative trait loci (QTL) mapping in an F population. A genetic linkage map that included 1,017 markers merged into 268 bins and covered 793.