Bioprocess Biosyst Eng
December 2024
ε-Poly-L-lysine (ε-PL) is a natural and wide-spectrum antimicrobial additive. In this study, the production of ε-PL by Streptomyces albulus FQF-24 using cassava starch (CS) as carbon source and the effects of different feeding methods were investigated in a fermenter. The initial shake flask experiments demonstrated the efficient production of ε-PL with CS, achieving the ε-PL production of 1.
View Article and Find Full Text PDFFood Sci Biotechnol
June 2024
Enzymatic preparation of rare sugars as an alternative to traditional sweeteners is an effective strategy to achieve a low-calorie healthy diet. Ribose-5-phosphate isomerase B (RpiB) is a key enzyme in the non-oxidative branch of the catalytic pentose phosphate pathway. Here, we investigated the potential of ZXL1 ( ZXL1) derived RpiB (CfRpiB) in D-allose preparation.
View Article and Find Full Text PDFGarlic ( L.), the underground bulb of the plant in the family Liliaceae, is a common and popular spice that has historically been used to prevent and treat many different diseases such as pain, deafness, diarrhea, tumors, and other healthy problems. Garlic essential oil contains a variety of organosulfur compounds, such as the most representative diallyl disulfides (DADS) and diallyl trisulfides (DATS), which have attracted great interest in medicine, food, and agriculture because of their rich biological activities.
View Article and Find Full Text PDFBioprocess Biosyst Eng
August 2022
The production of ε-poly-L-lysine (ε-PL) from cassava bagasse hydrolysate (CBH) by Streptomyces albulus US3-18 was investigated in this study. With 30 g/L glucose from CBH, 1.30 g/L ε-PL and 10.
View Article and Find Full Text PDFPrep Biochem Biotechnol
May 2022
ε-Poly-l-lysine (ε-PL) is used as a natural food preservative which consists of l-lysine units connected. However, due to the expensive culture medium, the production cost of ε-PL remains high. In this study, cheap raw materials cassava starch (CS) and fish meal (FM) were employed by FQC-24 for ε-PL production.
View Article and Find Full Text PDFA novel immobilized cellulase based on hydrogel microsphere was established to accommodate ionic liquid (IL)-involved in situ biomass saccharification. For immobilization, poly(N-isopropylacrylamide) (PNIPAM) was employed to immobilize cellulase, with immobilization yielded up to 95.6%.
View Article and Find Full Text PDFJ Microbiol Biotechnol
July 2018
In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened sp. CSL1, which could catalyze the isomerization reaction of rare sugars.
View Article and Find Full Text PDFJ Microbiol Biotechnol
April 2016
The mitogen-activated protein kinase HOG1 (high-osmolarity glycerol response pathway) plays a crucial role in the response of yeast to hyperosmotic shock. Trichosporonoides oedocephalis produces large amounts of polyols (,e.g.
View Article and Find Full Text PDFA cellulase producing strain was newly isolated from soil samples and identified as Paenibacillus sp. LLZ1. A novel aqueous-dimethyl sulfoxide (DMSO)/1-ethyl-3-methylimidazolium diethyl phosphate ([Emin]DEP)-cellulase system was designed and optimized.
View Article and Find Full Text PDFNat Prod Res
April 2007
Fibrarecisin (1), a novel triterpenoid, was isolated from chloroform extract of the stem bark of Fibraurea recisa Pierre. Its structure was elucidated by spectroscopic methods. Compound 1 exhibited significant antitumor activity in vitro against A-549 cancer cell at 10 microM by SRB and MTT methods.
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
July 2003
On the base of element and metablism balancing, the mathematical model of the cultivation process with Streptomyces aureofaciens was developed, and the unknown parameters in the model were estimated with the method of nonlinear optimization. Firstly the energetic coefficient of CTC biosynthesis was gained, which was 1.8 - 2.
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