Classification of mitral regurgitation in echocardiography based on deep learning methods.

Background: The classification of mitral regurgitation (MR) based on echocardiography is highly dependent on the expertise of specialized physicians and is often time-consuming. This study aims to develop an artificial intelligence (AI)-assisted decision-making system to improve the accuracy and efficiency of MR classification.

Methods: We utilized 754 echocardiography videos from 266 subjects to develop an MR classification model.

Multi-electrode catheter PFA: Computational modeling and in vivo validation of lesion dynamics under energy-dose variants.

Background And Objective: As a non-thermal ablation modality, pulsed field ablation (PFA) has been widely applied in solid tumor treatment and cardiac ablation. Multiple factors are known to influence the effects of PFA, including pulse parameters, catheter configurations, and tissue properties. However, limited attention has been paid to the impact of discharge modes on PFA.

Analysis of Echocardiography and Risk Factors Related to Prognosis in Adult Patients with Isolated Congenitally Corrected Transposition of the Great Arteries.

This study sought to echocardiographic manifestations and the related risk factors affecting the prognosis of isolated congenitally corrected transposition of the great arteries (CCTGA). A total of 143 patients (≥18 years of age) were diagnosed with isolated CCTGA at Anzhen Hospital. The patients were classified as the operation group and the non-operation group depending on whether they had undergone tricuspid valve surgery.

Pathogenicity of invasive and non-invasive CC4821 Neisseria meningitidis.

Neisseria meningitidis (N. meningitidis) is an opportunistic pathogen that typically colonizes the mucosal surfaces of the nasopharynx. Under certain conditions, N.

An Evaluation of the Safety, Immunogenicity, and Protective Efficacy of a Combined Diphtheria-Tetanus-Acellular Pertussis, Type b, and ACYW135 Meningococcal Conjugate Vaccine in Murine and Rat Models.

: The combined diphtheria-tetanus-acellular pertussis (three-component), type b (Hib, conjugate), and ACYW135 meningococcal (conjugate) vaccine (DTaP-Hib-MCV4) offers a promising alternative to single-component vaccines, potentially simplifying immunization schedules and improving vaccination coverage. : We evaluated the safety, immunogenicity, and protective efficacy of DTaP-Hib-MCV4 in animal models. Acute and long-term toxicity studies were conducted in Sprague-Dawley (SD) rats with equal numbers of male and female animals.

Predictive Factors of Fetal Cardiac Manifestations of Neonatal Lupus in Anti-SSA/Ro and/or SSB/La Antibody-Positive Pregnant Women.

The factors associated with the development of cardiac manifestations of neonatal lupus (cardiac-NL) in fetuses with positive anti-SSA/Ro and/or SSB/La (anti-SSA/SSB) antibodies are unclear. This study aimed to investigate the predictive factors of fetal cardiac-NL in anti-SSA/SSB antibody-positive pregnant women.A total of 669 pregnant women with positive anti-SSA/SSB antibodies were retrospectively included.

Liver-Targeted Tolerogenic Vaccines: A Nano-Membrane Coupled Approach for Autoimmune Disease Therapies.

The co-delivery of auto-antigens and immunomodulators for tolerance induction holds great potential in the treatment of autoimmune diseases. However, their efficacy in enriching antigens within target cells remains limited, and the use of immunomodulators poses a risk for systemic immunosuppression. Here, an apoptotic erythrocyte-derived membrane is developed for the first time, demonstrating its strong abilities to target hepatic antigen-presenting cells (APCs) and stimulate negative regulatory pathways.

Origin of clonal complex 4821.

() is the causative agent of human invasive meningococcal disease (IMD). Clonal complex (CC) 4821 is a unique genetic cluster of that emerged two decades ago in Anhui Province, China and became the predominant cluster. However, the evolutionary origin of CC4821 remains unclear.

Dietary microbiota-mediated shifts in gut microbial ecology and pathogen interactions in giant pandas (Ailuropoda melanoleuca).

The impact of dietary microorganisms on host microbiota is recognized, but the underlying mechanisms remain unclear. This study examined the effects of bamboo surface microbiota, including virulence factors, antibiotic resistance genes (ARGs), and mobile genetic elements from different bamboo parts (leaves, shoots, and culms), on giant panda gut microbiota using three pairs of twins. Results showed that bamboo and fecal samples shared 1670 microbial species, with shoot surface microbiota contributing the highest proportion (21%, Bayesian source tracking) of contemporaneous gut microbiota, primarily by increasing abundances of Escherichia coli and ARGs.

Four novel types of placental villous trees: Insights from SlowflowHD ultrasound imaging into placental microvascular architecture in congenital heart disease.

Objective: Placental vascular development is critical for maternal-fetal exchange, and altered fetal cardiovascular physiology in congenital heart disease (CHD) may impact placental circulation. This study aimed to assess whether SlowflowHD imaging technology could qualitatively evaluate alterations in placental vascular structure in CHD.

Methods: A cross-sectional study was conducted using SlowflowHD to image placental vasculature in 215 fetuses, normal (n = 106) and CHD (n = 109) including critical CHD (CCHD) (n = 66) and other fetal heart disease (FHD) (n = 43).

Rare damaging variants in the sex differences of congenital heart disease: an exome sequencing study.

Background: Congenital heart disease (CHD) exhibits a marked male predominance in birth prevalence, yet the genetic mechanisms underlying this sex disparity remain poorly understood. This study investigates the contribution of rare damaging variants on autosomes and the X chromosome to sex differences in foetal CHD.

Methods: Parents of foetuses with CHD were recruited for the study.

Acetylcholinesterase-assisted photoelectrochemical solution gated graphene field-effect transistor for organophosphates pesticide detection.

This study develops a photoelectrochemical solution-gated graphene field-effect transistor (PEC-SGGT) for ultrasensitive organophosphates (OPs) detection, merging optoelectronic modulation with enzymatic signal amplification. The sensor employs a hybrid system of cadmium sulfide quantum dots (CdS QDs) and acetylcholinesterase (AChE), using acetylthiocholine (ATCh) as the substrate. Under light, CdS QDs generate electron-hole pairs, while AChE hydrolyzes ATCh into thiocholine (TCh), which enhances charge separation and amplifies photocurrent.

Long-Term Prognosis of Postinfarction Ventricular Septal Rupture: A Single-Center Experience.

Objective: This seven-year study investigates risk factors for long-term mortality in AMI patients with ventricular septal rupture (VSR) to enhance clinical management and outcomes.

Methods: We conducted a retrospective cohort study of 180 AMI patients with VSR from January 2016 to October 2023. We collected data on demographics, clinical features, treatments, and outcomes.

Tough and waterproof microneedles overcome mucosal immunotolerance by modulating antigen release patterns.

Mucosal vaccines are considered an ideal choice for combating mucosal pathogens due to their ability to neutralize pathogens at the first line of defense. However, the development of mucosal subunit vaccines is constricted by rigorous challenges, such as low immunogenicity, poor antigen delivery efficiency, and mucosal tolerance. Here, a buccal microneedle patch incorporated with engineered nanoparticles loaded with urease B subunit (rUreB) was developed to overcome the above challenges.

Normal fetal echocardiography ratios - a multicenter cross-sectional retrospective study.

Objectives: Normal fetal echocardiography ratios reflect blood flow balance and developmental patterns, providing a basis for more accurate diagnosis and prediction of congenital heart disease in fetuses. Despite its significance, standardized studies with ample samples are lacking. We aim to establish reference ranges for fetal cardiac structural parameters and hemodynamics using extensive multicenter data, including 11 ratios, and to clarify how these ratios change with gestational age.

Evolution of transarterial chemoembolization-related liver abscess over time: a systematic review and meta-analysis.

Background: Transarterial chemoembolization (TACE) is a primary method for treating malignant liver tumors; however, the occurrence of liver abscesses after TACE has always been a concern. With the evolution of time, TACE techniques and practical experience continue to advance, leading to a deeper understanding of post-TACE liver abscesses. This meta-analysis aimed to comprehensively examine the occurrence of liver abscesses after TACE and focus on its changing trends.

RdxA-independent mechanism of metronidazole metabolism.

Introduction: Metronidazole (MNZ) is widely used to treat infection worldwide. However, due to excessive and repeated use, resistance rates have exceeded 90% in some regions. The mechanisms of MNZ resistance have been extensively studied, and RdxA has been identified as the primary enzyme responsible for MNZ activation.

Intestinal overexpression of alters the composition, diversity and function of gut microbiota in mice.

The intestinal microbiota is important for the health of the host and recent studies have shown that some genes of the host regulated the composition of the intestinal microbiota. Group 10 phospholipase A2 (PLA2G10) is a member of the lipolytic enzyme family PLA2, which hydrolyze the ester bond at the sn-2 position of phospholipids to produce free fatty acids and lysophospholipids. PLA2G10 is secreted into the intestinal lumen, but its impact on the gut microbiota remains unclear.

Assessment of Fetal Cardiac Morphology and Function in Pregnant Women With Systemic Lupus Erythematosus Using Speckle Tracking Echocardiography.

Purpose: Study aimed to assess fetal heart morphology and function in pregnancies with systemic lupus erythematosus (SLE) utilizing speckle tracking echocardiography (STE) and to evaluate indicators for predicting fetal subclinical cardiac damage in this population.

Methods: The study involved 99 SLE-affected fetuses and 99 gestational age-matched controls. The fetal cardiac morphology and function parameters were calculated using STE (FetalHQ).

Decoupled Classifier Knowledge Distillation.

Mainstream knowledge distillation methods primarily include self-distillation, offline distillation, online distillation, output-based distillation, and feature-based distillation. While each approach has its respective advantages, they are typically employed independently. Simply combining two distillation methods often leads to redundant information.

The reference ranges for fetal ductus venosus flow velocities and calculated waveform indices and their predictive values for right heart diseases.

Objectives: This study endeavors to establish comprehensive normal reference ranges and Z score formulas for ductus venosus (DV) flow velocity parameters and calculated waveform indices across different gestational ages in low-risk fetuses. Furthermore, we aim to validate the predictive capabilities of these Z score calculation formulas in fetuses with diverse right heart diseases.

Methods: A total of 8,953 singleton low-risk fetuses and 70 fetuses diagnosed with different types of right heart diseases were retrospectively enrolled.

Microneedle-delivered adeno-associated virus vaccine amplified anti-viral immunity by improving antigen-presenting cells infection.

Adeno-associated viruses (AAV) have significant potential as vaccine carriers due to their excellent biosafety and efficient antigen gene delivery. However, most AAV vaccines show limited capacity to transduce antigen-presenting cells (APCs) following intramuscular injection which may cause inadequate cellular immune responses and undesired side effects due to transducing other tissues or cells. Herein, we developed a soluble microneedle patch for targeting the AAV vaccines to the epidermal and dermal APCs.