A Versatile and Bioinspired Hierarchical Nanoflower Coating for Enhanced Photodriven Antimicrobial Efficacy against Foodborne Pathogens.

Contamination by pathogenic microbes frequently occurs on the surfaces of solid materials, posing serious threats to food safety and human health. To mitigate the spread of pathogens and reduce the risk of antimicrobial resistance, there is an urgent need for nanostructured surface modification materials that offer high bactericidal efficiency without relying on conventional antimicrobial agents or antibiotics. In this study, we report a green and facile approach for fabricating antimicrobial nanoflower (PDA-Cu NFs) coatings on solid substrates, including polystyrene, glass, and stainless steel via one-step biomimetic mineralization.

Mechanistic insights into volatile odor changes in surimi gels with typical cross-linking degrees during frozen storage based on lipidomics analysis.

This study investigated the potential mechanisms underlying odor changes in surimi gel during frozen storage, focusing on lipid alterations. Frozen storage significantly impacted the odor characteristics of surimi gel, with a noticeable decline in fish aroma and an intensification of fishy, oily, and earthy odors after 90 days of freezing. Using liquid nitrogen spray freezing at -80 °C and increasing the cross-linking degree to 62.

Investigation of the protective mechanisms of liquid nitrogen spray freezing and TGase cross-linking on the structural characteristics of surimi gels during frozen storage.

This study explored the mechanism of liquid nitrogen spray freezing and transglutaminase cross-linking in maintaining surimi gels' structure during storage. Results revealed that structure changes were, on the one hand, related to the growth and recrystallization of ice crystals during storage. Low cross-linking gels with air freezing exhibited minimum value after 20 days of storage, with hardness decreasing by 38.

Identification and Molecular Mechanism of Novel α-Glucosidase Inhibitory Peptides from the Hydrolysate of Hemp Seed Proteins: Peptidomic Analysis, Molecular Docking, and Dynamics Simulation.

There is a growing demand for natural and potent α-glucosidase inhibitors due to the rising prevalence of diabetes. In this study, newly identified α-glucosidase inhibitory peptides were identified from the tryptic hydrolysate of hemp seed proteins based on peptidomics and in silico analysis. A total of 424 peptides, primarily derived from four cupin-type-1 domain-containing proteins, were identified, and 13 ultimately were selected for validation based on their higher PeptideRanker scores, solubility, non-toxicity, and favorable ADMET properties.

Effects of Diet Xylooligosaccharide Supplementation on Growth Performance, Carcass Characteristics, and Meat Quality of Hu Lambs.

In this study, we examined the effect of xylooligosaccharide (XOS) supplementation on the growth performance, carcass characteristics, and meat quality of Hu lambs. In total, 60 Hu lambs (two months old and weighing 17.32 ± 0.

Evaluation of Meat Quality in Duhu Hybrid Lambs Reared in Different Conditions.

In the western Henan agricultural area, Duhu (Dupo♂ × Hu sheep♀) hybrid lambs are the primary breed of local meat sheep, predominantly raised in large-scale indoor feeding systems, although many farmers still rely on grazing. However, limited research exists on the meat quality of Duhu lambs under both grazing and indoor feeding systems. This study examined how grazing and indoor feeding affect the nutritional quality, flavor, amino acid profile, and fatty acid composition of 7-month-old Duhu lamb meat.

Prenylated flavonoid fractions from Glycyrrhiza glabra alleviate insulin resistance in HepG2 cells by regulating the ERK/IRS-1 and PI3K/Akt signaling pathways.

Insulin resistance (IR) is a key factor in the pathogenesis of disrupted glucose metabolism. Although the extract of Glycyrrhiza glabra has shown significant hypoglycemic activity, its bioactive components remain to be identified, and their mechanisms of action, especially on hepatocyte glucose metabolism, are yet to be explored. In the present study, the primary compounds from Glycyrrhiza glabra [named prenylated flavonoid fractions (PFFs)] have been identified and their chemical structures have been elucidated.

An Evaluation Model of Agricultural Entrepreneurial Ecological Environment Quality Oriented to Public Health Topic Using the Optimized Neural Network Algorithm.

As the primary industry, agriculture is a prerequisite for human survival and a basic industry of the national economy. As a result, the growth of agriculture is vital for the nation's socioeconomic development. The construction of ecological civilization and the reform and innovation of farmers' digital entrepreneurial behavior in the region are intimately correlated with the agricultural eco-environmental quality.

Screening and identification of a novel antidiabetic peptide from collagen hydrolysates of Chinese giant salamander skin: network pharmacology, inhibition kinetics and protection of IR-HepG2 cells.

In this study, a novel peptide GPPGPA was screened from the collagen hydrolysates of Chinese giant salamander () skin, and its anti-diabetes mechanism was predicted by network pharmacology, and an inhibitory effect on α-glycosidase and protective effect on IR (insulin resistance) and oxidative stress of IR-HepG2 cells were detected. Through network pharmacology screening, GPPGPA was found to have good drug-like properties, and 103 targets of GPPGPA overlap with T2DM targets. These targets were mainly enriched in the PI3K-Akt signaling pathway associated with T2DM, the AGE-RAGE signaling pathway in diabetic complications, the TNF signaling pathway, insulin resistance and so on.

The Evaluation Model of College Students' Mental Health in the Environment of Independent Entrepreneurship Using Neural Network Technology.

In recent years, the employment of college students is becoming more and more prominent; no matter for the society, universities, college students themselves, and their families have formed a huge pressure, in the current situation, the success rate of college students to start their own business is not high; one of the important reasons is that college students generally have defects in entrepreneurial psychology. Therefore, effective evaluation of college students' mental health under the environment of independent entrepreneurship is conducive to comprehensively improving the quality of talent training in colleges and universities. In this paper, we propose a novel three-channel multifeature fusion network based on neural network technology to identify and predict college students' mental health problems in the self-entrepreneurship environment.

Effects of aspirin on hepatocellular carcinoma and its potential molecular mechanism.

Purpose: To explore the effects of aspirin (ASP) on the proliferation and apoptosis of HepG2 hepatocellular carcinoma (HCC) cells via the Wnt/β-catenin signaling pathway.

Methods: Human HCC cells were cultured and treated with ASP at different concentrations. Cell proliferation was determined with cell counting kit-8 (CCK-8) and colony formation, and the rate of apoptosis was measured by flow cytometry.

Effects of konjac glucomannan on the water distribution of frozen dough and corresponding steamed bread quality.

The influences of konjac glucomannan (KGM) on the water distribution of frozen dough and steamed bread quaility made from frozen dough were addressed in this study. Low Field Nuclear Magnetic Resonance (LF-NMR) analysis demonstrated that the less tightly bound water of the dough was transferred to tightly bound water with the increase of KGM. Differential Scanning Calorimetry (DSC) showed that the freezable water of the frozen dough was transferred to non-freezable water with increasing the amount of KGM.

Interaction mechanism of aloe-emodin with trypsin: molecular structure-affinity relationship and effect on biological activities.

The molecular mechanism of interaction between aloe-emodin (AE) and trypsin was investigated, exhibiting remarkable outcomes. To detect the interaction mechanism, the binding of AE with trypsin was examined by a multi-spectroscopy and molecular docking method. Results showed that the binding of AE and trypsin would lead to static quenching and their binding forces were van der Waals forces and hydrogen bonding.

In Vitro Antioxidant Activity of Peptides from Simulated Gastro-Intestinal Digestion Products of Scale Gelatin.

A two-stage simulated gastro-intestinal (GI) digestion model (2 h pepsin treatment and subsequent 2 h pancreatin treatment at 37 °C) was used to explore the antioxidant activity of the digested products of scale gelatin with different molecular weights (MW). From the gastric phase to the intestinal phase, the hydrolysis degree of the products increased from 2.6 ± 0.

Molecular mechanism of the interaction between resveratrol and trypsin via spectroscopy and molecular docking.

The molecular mechanism of the interaction between resveratrol and trypsin was studied using fluorescence spectroscopy (intrinsic fluorescence, synchronous fluorescence, three-dimensional fluorescence), ultraviolet-visible (UV-vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and the molecular docking method, as well as through enzyme activity and antioxidant assay. The fluorescence experiments (the Stern-Volmer quenching constants (KSV)) indicated that resveratrol quenched the intrinsic fluorescence of trypsin through the static quenching mechanism. The number of binding sites was about one.

[Response of Nostoc flageliforme cell to Cu2+, Cr2+ and Pb2+ stress].

Objective: This study aimed to investigate the effects of Cu2+, Cr2+ and Pb2+ stress on Nostoc flagelliforme cell.

Methods: The response of Nostoc flagelliforme cell was analyzed under the stress. The modified BG11 culture medium containing different heavy metal ions of 0, 0.

Molecular switch for the assembly of lipophilic drug incorporated plasma protein nanoparticles and in vivo image.

A strategy to manipulate the disulfide bond breaking triggered unfolding, and subsequently assembly of human serum albumin (HSA) in a lipophilic drug-dependent manner is present. In this study, the hydrophobic region, a molecular switch of the HSA, was regulated to form HSA-paclitaxel (HSA-PTX) nanoparticles by a facile route. High-resolution transmission electron microscopy and fluorescence quenching indicate that HSA coassembled with PTX, which acts as a bridge to form core-shell nanoparticles about 50-240 nm in size, and that PTX might bind to the subdomain IIA sites of HSA.

Sustained delivery of endostatin improves the efficacy of therapy in Lewis lung cancer model.

The purpose of this work was to develop an effective delivery system for antiangiogenic therapy. Endostatin was microencapsulated into poly(lactic-co-glycolic acid) (PLGA) microspheres by using a w/o/o multiple emulsification-evaporation technique. Endostatin microspheres showed the encapsulation efficiency 100% with mean particle size about 25 microm.

A simple method for obtaining transferrins from human plasma and porcine serum: preparations and properties.

A simple method was described for the purification of serum transferrin (Tf) from human plasma and porcine serum with relative high yield and purity. The properties including purity, integrity, immunoreactivity and the receptor-binding ability of the proteins were studied by several assays, comprising spectrometry, SDS-PAGE, HPLC, Western blotting, urea electrophoresis, mass spectrometry and cytometry. Analysis from all the different aspects manifested that the proteins were of high purity.