Development of a quantitative genomic instability scoring system and a related competing endogenous RNA network in head and neck squamous cell carcinoma.

Background: Genomic instability (GI) is a hallmark of cancer and plays a crucial role in the progression of head and neck squamous cell carcinoma (HNSCC). This study aimed to quantitatively characterize GI features and construct a GI-related competing endogenous RNA (ceRNA) network in HNSCC.

Methods: Weighted gene co-expression network analysis (WGCNA) and differential gene expression analysis were conducted to compare genomically stable and unstable HNSCC samples.

Arabidopsis CIRP1 E3 ligase modulates drought and oxidative stress tolerance and reactive oxygen species homeostasis by directly degrading catalases.

Reactive oxygen species (ROS) plays critical roles in modulating plant growth and stress response and its homeostasis is fine tuned using multiple peroxidases. HO, a major kind of ROS, is removed rapidly and directly using three catalases, CAT1, CAT2, and CAT3, in Arabidopsis. Although the activity regulations of catalases have been well studied, their degradation pathway is less clear.

The high-quality genome of uncovers the genomic mechanism of high levels of schaftoside, a promising drug candidate for treatment of COVID-19.

Recent study has evidenced that traditional Chinese medicinal (TCM) plant-derived schaftoside shows promise as a potential drug candidate for COVID-19 treatment. However, the biosynthetic pathway of schaftoside in TCM plants remains unknown. In this study, the genome of the TCM herb (Osbeck) H.

Selection and validation of reference genes for RT-qPCR analysis in Merr.

Unlabelled: Gene expression valuated by reverse transcription-quantitative PCR (RT-qPCR) are often applied to study the gene function. To obtain accurate and reliable results, the usage of stable reference genes is essential for RT-qPCR analysis. The traditional southern Chinese medicinal herb, Merr is well known for its remarkable effect on the treatment of urination disturbance, urolithiasis, edema and jaundice.

Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1.

The present study was designed to observe the expression of the centrosomal protein 63 in papillary thyroid cancer (PTC) tissues and cells and to explore the clinical significance of Cep63 expression in PTC. Primary PTC tissues and matched normal thyroid tissues were collected, and the Cep63 expression level was determined by reverse transcription‑quantitative PCR and western blotting. A stable Cep63‑knockout cell line was constructed to assess the proliferation, invasion, migration and apoptosis abilities .

SQSTM1/p62 Promotes Cell Growth and Triggers Autophagy in Papillary Thyroid Cancer by Regulating the AKT/AMPK/mTOR Signaling Pathway.

Background: Thyroid cancer is one of the most common endocrine malignancies worldwide, and papillary thyroid cancer (PTC) is the most common pathologic type of thyroid cancer. SQSTM1/p62 activity mediates different biological functions. This study aimed to investigate the effect of SQSTM1/p62, a multifunctional receptor, on biological function and autophagy characteristics in the human PTC cell line TPC-1.

The Antitumour Effect of Extract on Thyroid Cancer Cells In Vitro and In Vivo.

, a traditional Chinese medicine, has been used to treat various benign and malignant tumours for centuries in China. In our previous studies, extract (PVE) was shown to promote apoptosis in differentiated thyroid cancer (DTC) cells. However, whether other mechanisms are involved in the antitumour effect of PVE in thyroid cancer (TC) cells remains unclear.

Generation and identification of a thyroid cancer cell line with stable expression of CCDC67 and luciferase reporter genes.

Coiled-coil domain containing 67 (CCDC67) gene is a tumor suppressor gene that exhibits a significant inhibitory effect on a variety of tumors. Our previous study demonstrated that the upregulation of CCDC67 gene in TPC-1 cells inhibited cell proliferation, migration and invasion, and promoted apoptosis . However, due to the lack of a suitable cell tool, these results were not validated .

Subcellular distribution of fluoranthene in Chlorella vulgaris with the presence of cetyltrimethylammonium chloride.

This study explored the possible mechanism of the joint toxicity of binary mixtures of cetyltrimethylammonium chloride (CTAC) and fluoranthene (Flu) to the green alga Chlorella vulgaris by examining the subcellular distribution of Flu within the alga. The joint action of CTAC (100 μg L(-1)) and Flu (0-200 μg L(-1)) on the algae changed from a synergetic effect (0-50 μg L(-1)) to an antagonistic effect (50-200 μg L(-1)) with an increase of the Flu concentration. The Flu uptake was enhanced by the presence of CTAC through the intracellular detection of Flu.

Joint action of binary mixtures of cetyltrimethyl ammonium chloride and aromatic hydrocarbons on Chlorella vulgaris.

The joint action of binary mixtures of cetyltrimethyl ammonium chloride (CTAC), a cationic surfactant, and six aromatic hydrocarbons (AHs) on green algae Chlorella vulgaris was investigated. In single systems, inhibition efficiency of CTAC on the growth of algae was much higher than that of AHs (benzene, toluene, phenol, nitrobenzene, phenanthrene and fluoranthene). In combined systems, the toxicity of CTAC was enhanced by low concentrations of AHs.

[Anesthetic management for cesarean section involving placenta previa].

Background: There are few consistent anesthetic guidelines how to manage cesarean section in the presence of placenta previa. Main problem may be hemorrhage, as occasionary unexpected massive bleeding leads to life-threatening hemorrhage.

Methods: We investigated retrospectively, covering the period between April 1, 2001 and September 30, 2005, 30 women with placenta previa who had undergone cesarean section.