Full-Length Transcriptome Analysis of Alternative Splicing and Polyadenylation in the Molecular Regulation of Labor Division in .

Honeybees are vital pollinators with functional differentiation as a key survival strategy. The Chinese honeybee () exhibits exceptional nectar foraging in complex terrains, yet how alternative splicing (AS) and polyadenylation (APA) regulate its labor division remains unclear. Here, we applied PacBio full-length transcriptome sequencing to annotate worker bee transcriptomes across three developmental stages (3d, 10d, 21d), calibrating the third-generation sequencing data with second-generation sequencing to enhance transcriptome annotation accuracy.

Exposure assessment for air-to-skin uptake of traditional and emerging organophosphate flame retardants.

Human exposure to organophosphate flame retardants (OPFRs) via direct air-to-skin uptake that may be comparable to or even exceed inhalation. Previous studies have mainly focused on traditional OPFRs (tOPFRs) without sufficient attentions to emerging OPFRs (eOPFRs), such as isopropylated or tert-butylated triaryl phosphate ester isomers (ITPs or TBPPs). This study aims to further explore the transport of both OPFRs through the skin, specifically the stratum corneum (SC).

Factors influencing healthcare workers' vaccine recommendations: A cross-sectional study in Zhejiang Province, China.

To investigate factors influencing vaccine recommendations by healthcare workers (HCWs) in Zhejiang Province, China. A cross-sectional survey was conducted among 569 HCWs to assess their beliefs and attitudes toward vaccines, vaccine recommendations and barriers to recommending vaccinations. Logistic regression was utilized to analyze influencing factors.

Combination of low-intensity pulsed ultrasound irradiating immune organs with immune checkpoint blockade augments systemic anti-tumor immunity on low tumor burden 4T-1 breast cancer.

Background: Despite achieving complete remission (CR) through surgery, chemoradiotherapy, targeted therapy, and other treatment modalities, breast cancer, particularly triple-negative breast cancer (TNBC) remains at a high risk of recurrence and metastasis. Enhancing anti-tumor immunity to eliminate residual tumor cells may reduce TNBC relapse. Our previous research indicated low-intensity pulsed ultrasound (LIPUS) can activate the anti‑tumor immunity.

High-resolution multispectral three-dimensional profiling using a metasurface-enhanced SPAD quanta image sensor.

This paper presents a high-resolution, single-photon LiDAR imaging camera capable of performing simultaneous depth and color estimation. It features a time-gated CMOS single-photon avalanche diode detector array with a large fill-factor, integrated with a metasurface-based plasmonic color filter array for three-dimensional color imaging. A sliding detector gate enables millimeter-scale depth resolution using as few as two binary planes per step, and high-fidelity color reconstruction is demonstrated from as few as two photons per pixel.

Correction: Severity, uncertainty, social support and coping style of parents who have children with epilepsy: a structural equation model.

[This corrects the article DOI: 10.3389/fneur.2025.

Determining the differences in O-NO-VOCs sensitivity between sauna and roast days.

Temperature is a key meteorological factor that affects tropospheric ozone (O), with both humid-heat (sauna days) and dry-heat (roast days) conditions leading to O exceedances. However, the mechanisms influencing O formation and degradation under these two weather conditions remain unclear. Therefore, experiments were conducted in Beijing from 2019 to 2021 to observe O, its precursors, and related meteorological elements.

Human neutrophil α-defensin HNP1 interacts with bacterial OmpA to promote Acinetobacter baumannii biofilm formation.

Acinetobacter baumannii is the causative agent of a wide range of nosocomial and community-acquired infections that remain extremely difficult to treat due largely to its antibiotic resistance contributed, in part, by biofilm formation. We find that the prototypic human neutrophil α-defensin HNP1, present in the bronchoalveolar lavage fluids from Acinetobacter baumannii-infected patients, promotes Acinetobacter baumannii biofilm formation through interactions with the bacterial outer membrane protein OmpA. As a result of HNP1-enhanced biofilm formation, Acinetobacter baumannii becomes more tolerant to antibiotics and more readily colonizes host cells and tissues.

Effects of Deformation Parameters on Phase Transformation of B1500HS High-Strength Steel During the Non-Isothermal Deformation Process.

To investigate the effects of deformation parameters on the phase transformation of B1500HS high-strength steel, non-isothermal deformation tests were conducted on a Thermomaster-Z thermal mechanical simulator under different conditions in this study. Qualitative and quantitative investigations were carried out by analyzing the dilatation curves, color metallograph, and hardness data of deformed specimens. The results indicate that deformation can promote the formation of non-martensite.

Severity, uncertainty, social support and coping style of parents who have children with epilepsy: a structural equation model.

Aim: To examine four variables in the model of perceived uncertainty in illness in northwestern China; to explore the relationship between severity, social support, illness uncertainty, and active coping in parents of children with epilepsy.

Design: A cross-sectional study design.

Reporting Method: The STROBE checklist was used to ensure the rigor in this study.

Response of formaldehyde to meteorology in Beijing: Primary or secondary contributions.

During high-temperature periods in summer, formaldehyde (HCHO) levels increase due to secondary production. However, recent studies have also shown a rise in the HCHO concentration in winter, but the underlying cause remains unclear. Here, HCHO observations in urban Beijing were conducted, the impact of meteorological differences between warm and cold seasons to HCHO concentrations was investigated.

Differences in mature human milk metabolic profiles based on delivery mode and parity.

Human milk (HM) is regarded as the gold standard for infant nutrition. The metabolite profiles of mature human milk (MHM) have been reported to be affected by maternal physiological conditions, lactation stage, and maternal diets. We collected MHM (3-6 months postpartum) from 32 healthy mothers with different parities and delivery modes.

Analysis of clinical audiological characteristics in children with Williams syndrome in China.

Background: Williams Syndrome (WS) is a neurodevelopmental disorder caused by microdeletion on chromosome 7. Hearing loss (HL) is common in this population but is rarely taken seriously. Previous studies had small sample sizes and mixed conclusions, and few studies have investigated HL in children with WS.

Characteristics of Early Language Development in Children With Williams Syndrome.

Williams Syndrome (WS) is a rare neurodevelopmental disorder characterized by dissonance in cognition and language. The purpose of this study was to investigate the characteristics of early language development in WS children. Seventeen children, aged 2-5 years, diagnosed with WS in the outpatient department of child healthcare in our hospital from December 2020 to June 2023, were included in this study.

Oxidation-Triggered Formation of Diradical Cations from Paramagnetic Molecules and Their Spin Density Evolution.

Controllable intramolecular spin-polarized flow refers to the manipulation of spin-polarized electron transport within molecules through externally applied stimuli, thereby modulating their intramolecular spin characteristics and magnetic properties. In this work, we designed and synthesized four paramagnetic molecules, PDTN-NN, PDTN-IN, PO-NN, and PO-IN, by introducing nitronyl nitroxide (NN) and iminonitroxide (IN) radicals into phenothiazine and phenoxazine frameworks. Remarkably, we successfully generated the corresponding radical-substituted radical cations (diradical cations) and controlled their spin density distributions (SDDs) through redox stimuli.

[Status and dietary factor correlations of metabolic syndrome in elderly aged 65 years and above in rural Beijing in 2023].

Objective: To explore the prevalence of metabolic syndrome(MS) and its association with dietary factors in elderly people aged 65 years and above in rural Beijing.

Methods: From May to June 2023, 600 elderly aged 65 and above were selected in Daxing District of Beijing using multi-stage cluster random sampling. After informed consent, basic information, physical status, dietary status, and nutritional and biochemical indicators were obtained.

MORTALITY OF SEPTIC SHOCK SECONDARY TO PEDIATRIC PRIMARY PERITONITIS PREDICTED BY RESPIRATORY QUOTIENT COMBINED WITH LACTATE: A SURVEY FROM TWO CHILDREN'S HOSPITALS IN NORTHWEST CHINA.

Background: Pediatric sepsis is a life-threatening condition, with extremely high incidence and mortality among critically ill children worldwide. Patients with septic shock are susceptible to intestinal complications due to altered blood flow distribution, and these complications often correlate directly with a poor prognosis. Early detection of low perfusion and appropriate resuscitation are critical components in the management of patients experiencing shock.

Danggui Buxue Decoction Alleviates Inflammation and Oxidative Stress in Mice with -Induced Mastitis.

(1) Background: Bovine mastitis is a lactational disease caused by infection and milk stagnation in the mammary glands. Danggui buxue decoction (DBD), a traditional remedy for blood tonification, anti-inflammation, and antioxidation, has not been used previously to treat mastitis. (2) Methods: In this study, an mastitis model was established by infecting lactating Kunming mice with clinically isolated bovine mastitis-derived .

Vaccine Hesitancy and Associated Factors Among Caregivers of Children With Special Health Care Needs in the COVID-19 Era in China: Cross-Sectional Study.

Background: Immunization is a cost-effective way to prevent infectious diseases in children, but parental hesitancy leads to low vaccination rates, leaving children at risk. Caregivers of children with special health care needs are more hesitant about vaccines than those of healthy children.

Objective: The aim of the study is to investigate the changes in caregivers' vaccination hesitation of children with special health care needs before, during, and after the COVID-19 pandemic in China and to identify associated factors for caregivers' attitudes toward National Immunization Program (NIP) and non-NIP vaccines.

Exploring the Causal Relationship Between Gut Microbiota and Pulmonary Artery Hypertension: Insights From Mendelian Randomization.

Background: Research into the "gut-lung" axis links gut microbiota to pulmonary artery hypertension (PAH). However, the mechanisms by which gut microbiota influence PAH remain unclear. We aimed to investigate the causal relationship between the gut microbiota and PAH using Mendelian randomization analysis, identify key microbiota and metabolites, and explore the regulatory role of associated genes in PAH pathogenesis.

Research advances in children's sleep and vitamin D levels.

In recent years, studies have revealed that vitamin D, a steroid hormone essential for calcium and phosphorus metabolism, also plays a role in sleep. Adequate levels of vitamin D have been linked to improved sleep quality in children and effective prevention of sleep problems. This report is a review and summary of research on the significance of sleep, the stages of children's sleep, and the impact of vitamin D levels on sleep problems.

MiR-214 inhibits NF-κB pathway activation to alleviate lipopolysaccharide-induced mastitis by targeting TRAF1.

Mastitis in dairy cows is defined by inflammation of mammary tissue, and represents a significant challenge in the dairy industry. The microRNA miR-214 is recognized as a key endogenous regulatory molecule with a critical role in inflammatory diseases. However, its involvement in the regulation of mastitis remains unclear.

The SEPALLATA-like gene in regulates flower development by interacting with other MADS-box subfamily genes.

MADS-box genes are classified into five categories: ABCDE, including , , , , and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. In addition, subcellular localization experiments showed that the protein was localized in the nucleus.

Alternative Splicing and Alternative Polyadenylation-Regulated Cold Stress Response of .

Temperature is a pivotal ecological factor in the regulation of insect survival and reproduction [...