Plasma lipidomic analysis reveals distinct lipid alterations in patients with pulmonary tuberculosis.

Objective: This study aimed to characterize the plasma lipidomic profile of patients with pulmonary tuberculosis (PTB), identify lipid species with potential diagnostic utility, and explore their associations with clinical parameters to inform future biomarker development and mechanistic understanding.

Methods: In a case-control study, 50 newly diagnosed PTB patients and 50 age- and sex-matched healthy controls (HC) were enrolled between April and June 2021. Plasma samples were analyzed using LC-MS/MS-based lipidomics.

Association of sdLDL-C/LDL-C ratio with atherosclerotic cardiovascular disease: a case-control study.

Background: Low-density lipoprotein cholesterol (LDL-C) and small dense low-density lipoprotein cholesterol (sdLDL-C) are key drivers of atherosclerotic cardiovascular disease (ASCVD) risk. This study aims to investigate the correlation between sdLDL-C/LDL-C ratio and ASCVD to provide new ideas for accurate prediction of ASCVD and identifying high-risk groups.

Methods: The data were derived from patients who visited the Affiliated Hospital of Inner Mongolia Medical University from January 2019 to July 2023.

Association between changes in high-density lipoprotein cholesterol and risk of cardiovascular disease.

Background: The present study was performed to determine the association between changes in the high-density lipoprotein cholesterol (HDL-C) concentration and incident cardiovascular disease (CVD).

Methods: Time-dependent Cox regression models were used to evaluate the association between changes in the HDL-C concentration and the risk of incident CVD. Participants were followed up from 2015 to 2021.

Risk analysis of blood pressure changes on cardiovascular disease in Chinese population.

The study aimed to assess the impact of changes in blood pressure on cardiovascular events in the Chinese population. It enrolled 33 179 Chinese participants aged ≥35 years (57.1% women) without CVD at baseline.

High-efficiency electrochemical nitrate reduction to ammonia via boron-doped hydroxyl oxide cobalt induced electron delocalization.

Electrochemical nitrate reduction to ammonia is a promising alternative strategy for producing valuable ammonia. This prospective route, however, is subject to a slow electrocatalytic rate, which resulted from the weak adsorption and activation of intermediate species, and the low density electron cloud of active centers. To address this issue, we developed a novel approach by doping boron into metal hydroxyl oxides to adjust the electronic structure of active centers, and consequently, led a significant improvement in the Faraday efficiency upto approaching 100 %, as well as an impressive ammonia yield upto approximately 23 mg/h mgcat at -0.

Meta-analysis of the association between interleukin-17 and ischemic cardiovascular disease.

Background: Interleukin-17 (IL-17) has been hypothesized to be involved in ischemic cardiovascular disease (ICVD). However, the association of IL-17 with ICVD remained unclear. The aim of this study was to systematically analyze the available evidence regarding the association between IL-17 and ICVD.

Effectiveness and safety of REBACIN as a non-invasive intervention for persistent high-risk human papillomavirus infection: A real-world prospective multicenter cohort study.

Background: We previously reported that REBACIN effectively eliminates persistent high-risk human papillomavirus (hrHPV) infection. Here, we conducted a prospective multicenter cohort study to evaluate the safety and effectiveness of REBACIN, taking into account factors such as specific hrHPV subtype and patient's age.

Methods: According to inclusion/exclusion criteria and participant willingness, 3252 patients were divided into REBACIN group while 249 patients into control group.

REBACIN inhibits E6/E7 oncogenes in clearance of human papillomavirus infection.

Unlabelled: Previous studies have demonstrated that REBACIN intervention eliminates persistent high-risk human papillomavirus (hrHPV) infection. The initial establishment and subsequent progression of cervical cancer mainly depends on two major oncogenes, E6/E7, and previous studies have proposed E6/E7 oncogenes as a target for therapeutic drug development. The aim of this study was to investigate and whether REBACIN inhibits E6/E7 oncogenes for elucidating the mechanism of REBACIN in the clearance of persistent hrHPV infection.

Case Report: Noninvasive Clinical Intervention of REBACIN® on Histologic Regression of High Grade Cervical Intraepithelial Neoplasia.

High-risk human papillomavirus (hrHPV) persistent infection is the major cause of cervical cancer. Clinical intervention of hrHPV-associated high-grade squamous intraepithelial lesion (HSIL) is critical to prevent cervical cancer, and current treatment is surgery (an invasive therapy). However, some patients refuse to do so for an afraid of potential adverse effects on future fertility or other concerns which creates a critical need for development of non-invasive therapeutic strategies.

REBACIN® as a noninvasive clinical intervention for high-risk human papillomavirus persistent infection.

The development of highly sensitive HPV-genotyping tests has opened the possibility of treating HPV-infected women before high-grade lesions appear. The lack of efficient intervention for persistent high-risk HPV infection necessitates the need for development of novel therapeutic strategy. Here we demonstrate that REBACIN®, a proprietary antiviral biologics, has shown potent efficacy in the clearance of persistent HPV infections.

Extraction of high-quality RNA from rubber tree leaves.

A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact.

Chromogranin A deficiency in transgenic mice leads to aberrant chromaffin granule biogenesis.

The biogenesis of dense-core secretory granules (DCGs), organelles responsible for the storage and secretion of neurotransmitters and neuropeptides in chromaffin cells, is poorly understood. Chromogranin A (CgA), which binds catecholamines for storage in the lumen of chromaffin granules, has been shown to be involved in DCG biogenesis in neuroendocrine PC12 cells. Here, we report that downregulation of CgA expression in vivo by expressing antisense RNA against CgA in transgenic mice led to a significant reduction in DCG formation in adrenal chromaffin cells.

Disruption of a receptor-mediated mechanism for intracellular sorting of proinsulin in familial hyperproinsulinemia.

In familial hyperproinsulinemia, specific mutations in the proinsulin gene are linked with a profound increase in circulating plasma proinsulin levels. However, the molecular and cellular basis for this disease remains uncharacterized. Here we investigated how these mutations may disrupt the sorting signal required to target proinsulin to the secretory granules of the regulated secretory pathway, resulting in the unregulated release of proinsulin.

Mechanism of sorting proopiomelanocortin and proenkephalin to the regulated secretory pathway of neuroendocrine cells.

Proopiomelanocortin (POMC) and proenkephalin (PE) are synthesized at the endoplasmic reticulum and transported to the trans-Golgi network (TGN) where they are sorted and packaged into dense-core granules of the regulated secretory pathway (RSP). The mechanism of sorting POMC and PE to the RSP in neuroendocrine cells was investigated. Consensus sorting signals comprising two acidic residues and two hydrophobic residues exposed on the surface of N-POMC(1-26) and N-PE(1-32) were identified and shown to be sufficient and necessary for targeting POMC and PE to the RSP in PC12, Neuro2a, and AtT-20 cells.

Sorting of carboxypeptidase E to the regulated secretory pathway requires interaction of its transmembrane domain with lipid rafts.

Carboxypeptidase E (CPE) functions as a regulated secretory pathway sorting receptor for several prohormones, including pro-opiomelanocortin (POMC), proenkephalin and proinsulin. The association of CPE with lipid rafts in the trans -Golgi network and secretory granule membranes is necessary for its sorting receptor function. We now provide evidence that a domain within the C-terminal 25 residues of CPE functions as a signal for both raft association and the sorting of CPE to the regulated secretory pathway.