Transformation of a covalent organic framework from dual pores to single pores and their different properties.

We have achieved a controllable transformation of covalent organic framework (COF) isomers from BATD-Dma-COF-K (dual pores) to BATD-Dma-COF-R (single pores). The fluorescence of BATD-Dma-COF-K was enhanced by clinafloxacin (CFN). After transformation from dual pores to single pores, BATD-Dma-COF-R can effectively adsorb CFN, but cannot fluoresce to detect CFN.

Gut microbiota-mediated causal relations between sleep disorders and stroke: a Mendelian randomization study.

Background: Sleep disorders are closely linked to stroke, while the role of gut microbiota in human health and disease is increasingly recognized. However, the causal relationships between sleep disorders, gut microbiota, and stroke remain unclear.

Objective: This study aimed to investigate the causal relationships between sleep disorders, gut microbiota, and stroke (including its subtypes), as well as the potential mediating role of gut microbiota in the association between sleep disorders and stroke.

AIMP1 exerts hearing protection role in age related hearing loss mice by regulating SIRT1 expression.

Background: Age related hearing loss (ARHL) is a sensorineural hearing disease caused by multiple factors and its pathogenesis is still unclear. This work aims to investigate the precise role of AIMP1 in ARHL.

Methods: HEI-OC1 cells were treated with 1 mM HO for 2 h to induce cell damage.

Effect of crop type shift on soil C:N:P stoichiometry in a typical Yellow River irrigated district.

The Hetao irrigation area is one of the largest irrigation areas in the Yellow River Basin and is a typical salinized agricultural area. Crop type shifts can affect soil stoichiometric ratio characteristics, indicating soil nitrogen and phosphorus availability and the soil degradation process. However, few studies have been conducted in this area.

The new SCCmec type methicillin-resistant Staphylococcus aureus carried CRISPR-cas system isolated from a pig in China.

Methicillin-resistant Staphylococcus aureus (MRSA) lineages circulate globally in healthcare, community, and livestock-associated (LA) settings. Nine MRSA isolates were recovered from swine in China, all exhibiting resistance to ampicillin and multidrug resistance phenotypes. Among eight ST9-t899 isolates, SCCmec type XII(9C2) predominated.

Effect of Crop Type Shift on Soil Phosphorus Morphology and Microbial Functional Diversity in a Typical Yellow River Irrigation Area.

The Hetao irrigation area is one of the largest irrigation areas in the Yellow River Basin and a typical salinized agricultural area. Crop type shifts in this area can alter soil phosphorus (P) morphology and microbial functional diversity, thereby influencing soil P losses. However, few studies have elucidated the underlying mechanisms.

Association between arousal index during REM sleep and the incidence of atrial fibrillation in patients with obstructive sleep apnea.

Background: Accumulating studies have linked obstructive sleep apnea (OSA) to the occurrence of atrial fibrillation (AF). However, data on the relationship between sleep architecture parameters in OSA patients and the occurrence of AF are limited.

Objective: This exploratory study examined the association between 14 objectively collected sleep architecture parameters and the incidence of AF in OSA patients.

Machine learning-based predictive model for enteral nutrition-associated diarrhea in ICU patients and its nursing applications.

Background: Enteral Nutrition-Associated Diarrhea (ENAD) is a common complication in critically ill patients, significantly impacting clinical outcomes. Accurately predicting the risk of ENAD is crucial for early intervention and improving patient care.

Objective: This study aims to develop and validate a machine learning (ML)-based risk prediction model for Enteral Nutrition-Associated Diarrhea (ENAD) in ICU patients, and explore its application in nursing practice.

Comparative Study of Chemical Compositions and Antioxidant Capacities of Oils Obtained from Sixteen Oat Cultivars in China.

The global oat harvest area occupied by China has been increasing annually. In this study, the fatty acid and triacylglycerol compositions, lipid concomitants, and antioxidant capacities of 16 oat oil cultivars in China were compared. All oat oils were found to be rich in unsaturated fatty acids (UFA), particularly oleic acid and linoleic acid.

Genomic epidemiology and characterization of isolates from raw milk in Jilin, China: evidence of contamination with -positive, -positive, and -positive strains and human-livestock transmission.

Jilin province is the primary region for dairy cow and milk production in China. However, there are few reports on the genetic characteristics, antibiotic resistance, and prevalence of () in raw milk. Between 2021 and 2022, researchers identified 214 strains, with a prevalence rate of 42.

Molecular insights into the unique activation and allosteric modulation mechanisms of the human mas-related G-protein-coupled receptor X1.

MRGPRX1 plays dual roles in mediating nociception and pruritus, making it a promising target for alleviating itch and inhibiting pain. However, the mechanisms underlying MRGPRX1 activation and allosteric modulation remain poorly understood, posing significant challenges for structure-based drug discovery. Here, we employ Gaussian accelerated molecular dynamics (GaMD) for enhanced sampling of MRGPRX1, successfully obtaining a stable inactive conformation of the unbound receptor for the first time.

Porcine Epidemic Diarrhea Virus Is Inhibited by GS-441524 During an In Vitro Infection.

Porcine epidemic diarrhea virus (PEDV), the etiology of porcine epidemic diarrhea (PED), continues to impose severe economic burdens on pig farms in China. The continuous emergence of new variant strains makes it difficult for vaccinated sows to provide protective immunity to piglets. Hence, there is an urgent need for efficacious therapeutic drugs in clinical practice.

One-pot synthesized multifunctional Zn-MOF/HOF heterostructure sensor array assisted by machine learning for efficient capture, target discrimination and optosmart sensing of doxycycline analogs.

The ideal multifunctional platform that combines the capabilities of effective capture, sensitive detection, and accurate identification of doxycycline analogs (DCs) remains a serious challenge for ensuring the environment and food security. This work constructs heterostructure Zn-MOF/HOF asynchronous response fluorescence sensor using a multicomponent one-pot method for high-efficiency capturing and sensitive detecting DCs. Metal nodes and functional groups in Zn-MOF/HOF provide sites for specifically recognizing and sensitizing DCs that induce asynchronous response with blue/green fluorescence emission.

An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum.

PfAgo, a thermophilic Argonaute nuclease from Pyrococcus furiosus, is widely used in various fields due to its high DNA-guided DNA cleavage activity. However, its high-temperature-dependent cleavage activity largely restricts its applications in moderate-temperature scenarios. In this study, PfAgo is engineered for cold adaptation based on its ternary complex structure and the attributes of cold-adapted enzymes, yielding a series of variants with better performance at moderate temperatures.

Behavior recognition technology based on deep learning used in pediatric behavioral audiometry.

This study aims to explore the feasibility and accuracy of deep learning-based pediatric behavioral audiometry. The research provides a dedicated pediatric posture detection dataset, which contains a large number of video clips from children's behavioral hearing tests, encompassing various typical hearing test actions. A detection platform based on this dataset is also constructed, named intelligent diagnostic model of pediatric hearing based on optimized transformer (DoT); further, an estimation model of patient skeletal keypoints based on optimized transformer (POTR) was proposed to estimate human skeleton points.

Multifunction hydrogen-bonded organic framework aerogel platform for detection and removal of heavy metal ions in pear juice.

Heavy metal ions (HMIs) in food pose significant health risks due to their bioaccumulation and toxicity, necessitating effective detection and remediation methods. This study explores a multifunctional hydrogen-bonded organic framework (HOF-16) wrapped in sodium alginate (SA) to form a stable HOF-16/SA aerogel for detecting and removing HMIs in pear juice. The HOF-16 fluorescence probe exhibits excellent sensitivity with limits of detection of 0.

Air pollution and adult hospital admissions for ischemic stroke: a time-series analysis in Inner Mongolia, China.

Background: Previous studies have demonstrated that short-term exposure to ambient particulate matter elevates the risk of ischemic stroke in major urban areas of various countries. However, there is a notable gap in research focusing on remote areas inhabited by ethnic minorities and the cumulative effects of air pollutants. Our study conducted in the area aims to explore the potential association between ischemic stroke and air pollutants and contribute to improving health outcomes among the community.

Multistage characterization of label-derived oleamide: chromatographic purification with NMR structural elucidation and UPLC/Q-TOF-MS quantitation for pharmaceutical packaging compatibility assessment.

Pharmaceutical labeling is widely used for drug information identification, though compatibility risks persist despite no direct drug contact. This migration risk arises from the semi-permeable nature of primary packaging materials, allowing label additives to permeate into drug formulations. A substantial number of emerging additives, either novel therapeutic agents or structural mimics of prohibited substances, hinder unambiguous liquid chromatography mass spectrometry (LC-MS) identification.

The Effects of Varying Concentrations of Didecyl Methylpropyl Ammonium Iodide (DMPAI) on the Structure and Function of Soil Bacterial Communities in the Lake-Terrestrial Ecotone.

To address freshwater lake blooms resulting from eutrophication, the application of quaternary ammonium compounds as algaecides serves as an effective emergency remediation strategy. Didecyl methylpropyl ammonium iodide (DMPAI) is a novel quaternary ammonium algaecide; however, its bacteriostatic properties may significantly disrupt the microbial activity in lakes, particularly within the lake-terrestrial ecotone. To investigate the degradation process of DMPAI in the lake-terrestrial ecotone and its impact on the microbial community, experiments were conducted using a large-scale indoor simulation device to analyze DMPAI concentrations, the composition of the lake microbial community, and associated gene functions.

Expression of SLC2A1 is upregulated in a mouse model of age-related hearing loss.

Background: Age-related hearing loss (ARHL) is a common issue associated with aging. One of the typical causes of hearing loss is the irreversible death of hair cells. In addition, oxidative stress contributes to ARHL.

Expression of Cancer-Testis Antigens MAGE-A1, MAGE-A4, NY-ESO-1 and PRAME in Bone and Soft Tissue Sarcomas: The Experience From a Single Center in China.

Objective: Sarcomas are a heterogeneous group of malignancies, low disease-control levels and the limited durability of responses have prompted the exploration of various novel immunotherapeutic approaches. To preliminarily explore the feasibility of cancer vaccines based on cancer testis antigen in the immunotherapy of sarcomas, we investigate the expression of Cancer/Testis Antigens (CTA) MAGE-A4, PRAME, MAGE-A1, KK-LC-1, and NY-ESO-1 in bone and soft tissue sarcomas, with the aim of assessing their potential for use in sarcoma immunotherapy and determining their expression levels in different subtypes.

Methods And Results: We employed immunohistochemistry and multiplex immunostaining microarrays (MI chips) to assess the expression of MAGE-A4, PRAME, MAGE-A1, KK-LC-1, and NY-ESO-1 in 21 cases of undifferentiated pleomorphic sarcoma (UPS), 26 cases of smooth muscle sarcoma, 28 cases of liposarcoma, 40 cases of osteosarcoma (OS), and 13 cases of chondrosarcoma.

An injectable gambogic acid loaded nanocomposite hydrogel enhances antitumor effect by reshaping immunosuppressive tumor microenvironment.

Gambogic acid(GA)is a natural compound that exhibits strong antitumor activity against a variety of tumors. However, its poor water solubility, low specificity, and high toxicity lead to inevitable systemic adverse effects. To minimize side effects, combining gambogic acid (GA) with delivery systems such as nanohydrogels to develop an in situ vaccine system (ISV) shows great promise.

The interactive application and impacts of iron/nitrogen biogeochemical cycling in distributed ponds for non-point source pollution control in a watershed.

The linkages of distributed ponds are utilized in conjunction with one another to remediate non-point source (NPS) pollution in a water-scarce basin. This study provides an overview of a state-of-the-art thorough evaluation of ponds, which offers insight into the majority of topics covered by the ongoing scientific studies, including their various functions and factors affecting their functioning on the hydrological, physicochemical, and biological processes, such as environmental climate factors and basin-specific landscape configuration parameters, as well as process parameters for design, operation and management aspects. The linkages of ponds provide a variety of sustainable services (6R functions), such as resources, restoration, reduction, reuse, recycling, and recovery.

Enzymatically responsive nanocarriers targeting PD-1 and TGF-β pathways reverse immunotherapeutic resistance and elicit robust therapeutic efficacy.

Immune checkpoint inhibitors (ICIs) have revolutionized lung cancer treatment, yet resistance remains a challenge. Co-inhibition of PD-1/PD-L1 and TGF-β shows promise but faces limited efficacy and systemic toxicity. We developed gelatinase-responsive nanoparticles (GPNPs) delivering anti-PD-1 antibody (αPD-1) and TGF-β receptor I inhibitor galunisertib (Gal).