TDEase: An Open-Source Data Visualization Software Framework for Targeted Proteoform Characterization by Top-Down Proteomics.

Top-down proteomics (TDP) is a powerful approach for characterizing intact protein molecules and their diverse proteoforms. Despite recent advances, current TDP software tools often suffer from fragmented workflows, steep learning curves for non-experts, or limited interactive visualization capabilities. To address these challenges, we introduce TDEase, an integrated analytical framework designed to streamline and enhance TDP data interpretation, with a current focus on integration with the TopPIC suite package for targeted proteoform characterization.

A pattern and lock strategy integrating acoustic patterning and hydrogel crosslinking for stable cell architectures.

Cell patterning technology faces critical limitations in dynamic control, biocompatibility, and structural stability for reconstructing native tissues. Here, we establish an acoustic-hydrogel integration strategy that overcomes these challenges through synergistic physical-biological programming. Experimental validation using particle/red blood cells-patterned hydrogels demonstrated exceptional structural stability under physiological conditions.

A Bioinspired Piezoelectric Stress Buffer Layer for SiO-Based Electrodes Toward High-Energy Lithium Batteries.

High-specific-capacity silicon suboxide (SiO, 0 < x < 2) anodes have long faced the problems of huge volume expansion, fast capacity decay and unsatisfied rate performance. To overcome these bottlenecks, the volume expansion resistance and electrogenic Na transport functions of common rain frog (Breviceps adspersus) epidermis are introduced into the design philosophy of stress buffers for SiO electrodes. Thereupon, a mechanically robust, piezoelectric (MP) stress buffer layer comprised of ferroelectric tetragonal BaTiO nanoparticles and a novel homopolymer (PCM) binder of cyanoethyl carbamate-containing methacrylate is developed.

Transarterial chemoembolization combined with tyrosine kinase inhibitors and/or immune checkpoint inhibitors induced hypothyroidism is associated with improved overall survival in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) treatments, including transarterial chemoembolization (TACE) and systemic therapies (tyrosine kinase inhibitors [TKIs]/immune checkpoint inhibitors [ICIs]) are linked to hypothyroidism. This study aims to elucidate the clinical significance of treatment-induced hypothyroidism within a real-world cohort. We enrolled 130 HCC patients with baseline thyroid function measurements, and stratified into two cohorts: TACE monotherapy (n = 50) or TACE combined with TKIs/ICIs (n = 80).

A Mechanically Robust In-Situ Solidified Polymer Electrolyte for SiO-Based Anodes Toward High-Energy Lithium Batteries.

Silicon suboxide (SiO, 0 < x < 2) is an appealing anode material to replace traditional graphite owing to its much higher theoretical specific capacity enabling higher-energy-density lithium batteries. Nevertheless, the huge volume change and rapid capacity decay of SiO electrodes during cycling pose huge challenges to their large-scale practical applications. To eliminate this bottleneck, a dragonfly wing microstructure-inspired polymer electrolyte (denoted as PPM-PE) is developed based on in-situ polymerization of bicyclic phosphate ester- and urethane motif-containing monomer and methyl methacrylate in traditional liquid electrolyte.

Therapeutic potential of Sweroside in postmenopausal osteoporosis: Inhibition of osteoclast differentiation and promotion of osteoclast apoptosis via NF-κB and MAPK pathways.

Postmenopausal osteoporosis (PMOP) is a common metabolic bone disorder resulting from disrupted bone remodeling due to estrogen deficiency, leading to an increased risk of fractures in postmenopausal women. Sweroside, a natural compound derived from lonicerae japonicae flos, has demonstrated neuroprotective and antioxidant properties, yet its effects on PMOP remain underexplored. In vitro studies indicated that Sweroside inhibited osteoclast differentiation and bone resorption in a concentration-dependent manner.

Smart gel polymer electrolytes enlightening high safety and long life sodium ion batteries.

The overall performance of sodium-ion batteries, particularly regarding safety and cycle life, remains below expectations due to severe degradation of electrode materials and the electrode/electrolyte interphase. Herein, we develop a smart gel polymer electrolyte for hard carbon||NaNiFeMnO batteries through the in situ radical polymerization of a cyanoethylurea-containing methacrylate monomer and an isocyanate-based methacrylate monomer in conventional NaPF-carbonate-based electrolytes. We demonstrate that the smart gel polymer electrolyte facilitates the formation of robust electrode/electrolyte interphase layers, thus improving the thermal and chem-electrochemical stability of the electrodes.

An Electrode-Crosstalk-Suppressing Smart Polymer Electrolyte for High Safety Lithium-Ion Batteries.

Electrode crosstalk between anode and cathode at elevated temperatures is identified as a real culprit triggering the thermal runaway of lithium-ion batteries. Herein, to address this challenge, a novel smart polymer electrolyte is prepared through in situ polymerization of methyl methacrylate and acrylic anhydride monomers within a succinonitrile-based dual-anion deep eutectic solvent. Owing to the abundant active unsaturated double bonds on the as-obtained polymer matrix end, this smart polymer electrolyte can spontaneously form a dense crosslinked polymer network under elevated temperatures, effectively slowing down the crosstalk diffusion kinetics of lithium ions and active gases.

Effect of sludge humic acid-derived nano-biochars on anaerobic degradation of sulfamethoxazole by Shewanella oneidensis MR-1.

Some nano-biochars (nano-BCs) as electron mediators could enter into cells to directly promote intracellular electron transfer and cell activities. However, little information was available on the effect of nano-BCs on SMX degradation. In this study, nano-BCs were prepared using sludge-derived humic acid (SHA) and their effects on SMX degradation by Shewanella oneidensis MR-1 were investigated.

Covalently Cross-Linked Chemistry of a Three-Dimensional Network Binder at Limited Dosage Enables Practical Si/C Composite Electrode Applications.

Currently, Si (or SiO, 1 < < 2) and graphite composite (Si/C) electrodes (e.g., Si/C450 and Si/C600 with specific capacities of 450 and 600 mAh g at 0.

Banking sectors and carbon neutrality goals: mediating concern of financial inclusion.

Since industrialization, GHGs have steadily grown, and climate change threatens human civilization. The Chinese government actively engages in the administration of the global environment and has suggested that carbon neutrality be attained by 2060. Regional communities must understand their current carbon neutrality status and objectively design a course to attain carbon neutrality due to significant regional development disparities.

Rigid-tough Coupling of the Solid Electrolyte Interphase Towards Long-Life Lithium Metal Batteries.

Lithium metal batteries are highly pursued for energy storage applications due to superior energy densities. However, fast battery decay accompanied by lithium dendrite growth occurs mainly owing to solid electrolyte interphase (SEI) failure. To address this, a novel functional quasi-solid-state polymer electrolyte is developed through in situ copolymerization of a cyclic carbonate-containing acrylate and a urea-based acrylate monomer in commercial available electrolyte.

Object Detection Based on Roadside LiDAR for Cooperative Driving Automation: A Review.

Light Detection and Ranging (LiDAR) technology has the advantages of high detection accuracy, a wide range of perception, and not being affected by light. The 3D LiDAR is placed at the commanding height of the traffic scene, the overall situation can be grasped from the perspective of top view, and the trajectory of each object in the traffic scene can be accurately perceived in real time, and then the object information can be distributed to the surrounding vehicles or other roadside LiDAR through advanced wireless communication equipment, which can significantly improve the local perception ability of an autonomous vehicle. This paper first describes the characteristics of roadside LiDAR and the challenges of object detection and then reviews in detail the current methods of object detection based on a single roadside LiDAR and multi-LiDAR cooperatives.

Circular RNA circTTBK2 facilitates non-small-cell lung cancer malignancy through the miR-873-5p/TEAD1/DERL1 axis.

The underlying mechanisms by which circular RNAs (circRNAs) regulate non-small-cell lung cancer (NSCLC) progression remain elusive. This study investigated the role of circRNA circTTBK2 in NSCLC tumorigenesis. Quantitative reverse transcriptase polymerase chain reaction analysis of circTTBK2 in NSCLC tissues and cell lines was performed.

Direct Access to Bowl-Like Nanostructures with Block Copolymer Anisotropic Truncated Microspheres.

Bowl-like nanostructures have attracted significant scientific and technological interest due to their favorable characteristics, such as high specific surface area, interconnected porous channels, and conductivity. However, tailored synthesis of bowl-like nanostructures with well-defined and uniform morphology is still a challenge. Herein, we report a versatile microemulsion assembly approach to prepare bowl-like nanostructures of three different materials: polymer, carbon, and platinum.

A genome-wide single nucleotide polymorphism scan reveals genetic markers associated with fertility rate in Chinese Jing Hong chicken.

The function of the sperm storage tubules is directly correlated with the fertility of laying hens. However, little is known about the molecular mechanisms regulating the fertility traits in chicken. To identify genetic markers associated with reproductive traits, we calculated fertility rate at 61 to 69 wk (51 D) of Jing Hong chickens parent generation as the phenotype and the genotype were detected by the chicken 600K Affymetrix Axiom High Density single nucleotide polymorphisms (SNP)-array.

Association of single nucleotide polymorphism at long non-coding RNA 8138.1 with duration of fertility in egg-laying hens.

A previous genome-wide transcriptional analysis identified long non-coding RNA 8138.1 () as a candidate gene related to hen duration of the fertility (DF) trait. gene response to growth factor and reproductive system development suggests it has a vital role in reproduction.

Novel Polymorphisms in Gene Associated with Egg-Laying Rate in Chinese Jing Hong Chicken using Genome-Wide SNP Scan.

The improvement of egg production is of vital importance in the chicken industry to maintain optimum output throughout the laying period. Because of the elongation of the egg-laying cycle, a drop in egg-laying rates in the late laying period has provoked great concern in the poultry industry. In this study, we calculated the egg-laying rate at weeks 61-69 (60 days) of Jing Hong chickens parent generation as the phenotype, and the genotype were detected by the chicken 600K Affymetrix Axiom High Density (HD) Single Nucleotide Polymorphisms (SNP)-array.

Using Fluorescence Quenching Titration to Determine the Orientation of a Model Transmembrane Protein in Mimic Membranes.

After synthesis of transmembrane proteins (TMPs), they are transferred and inserted into plasma membranes to play biological functions. Crucially, orientation of TMPs in membranes determines whether they have biological activities. In cellular environments, a number of cofactors, such as translocon, can assist TMPs to be inserted into membranes in defined orientations.

Identifying artificial selection signals in the chicken genome.

Identifying the signals of artificial selection can contribute to further shaping economically important traits. Here, a chicken 600k SNP-array was employed to detect the signals of artificial selection using 331 individuals from 9 breeds, including Jingfen (JF), Jinghong (JH), Araucanas (AR), White Leghorn (WL), Pekin-Bantam (PB), Shamo (SH), Gallus-Gallus-Spadiceus (GA), Rheinlander (RH) and Vorwerkhuhn (VO). Per the population genetic structure, 9 breeds were combined into 5 breed-pools, and a 'two-step' strategy was used to reveal the signals of artificial selection.

Novel copy number variation of the TGFβ3 gene is associated with TGFβ3 gene expression and duration of fertility traits in hens.

Improvements in the duration of fertility (DF) could increase the interval between successive artificial inseminations, thereby decreasing the cost associated with production of hatching eggs. The molecular mechanisms involved in DF in hens remains under-explored. In this study, expression levels of the transforming growth factor-β genes (TGFβs: TGFβ1, TGFβ2, TGFβ3) were investigated in utero-vaginal junctions (UVJs) of hens with long DF (Group L, n = 10) and short DF (Group S, n = 10).