Clinicopathological and prognosis analysis of intraductal papillary neoplasm of the bile duct: A dual-center retrospective study.

Background: The precise clinical features and prognosis of IPNB remain challenging to define. More research is needed to enhance understanding of the disease.

Methods: The study retrospectively analyzes the clinical data of 123 patients with IPNB from two medical centers during January 2013 and May 2024.

Nanopore-Based Electroporation Enables High-Efficiency, Rapid RNA-Mediated Reprogramming of Primary Fibroblasts into Human iPSCs.

Induced pluripotent stem cells (iPSCs) have revolutionized regenerative medicine by overcoming the ethical and immunological challenges posed by embryonic stem cells, while nonviral reprogramming of fibroblasts to iPSCs often faces challenges in efficiency and complexity. We present a nanopore electroporation (NanoEP) system for efficient delivery of circular RNA (circRNA) encoding six reprogramming factors (OCT4, SOX2, KLF4, c-MYC, LIN28A, and NANOG) into human fibroblasts. NanoEP, employing engineered polycarbonate membranes for localized permeabilization, achieves >90% transfection efficiency at low voltages, enhancing cell viability and minimizing proinflammatory cytokine release (IL-6 and IL-18) compared to other methods.

T1-Hyperintense Cystic Renal Masses: MR Subtraction Imaging May Improve Interobserver Agreement and Diagnostic Performance in the Bosniak Classification.

Background: T1-hyperintensity in cystic renal masses (CRMs) complicates Bosniak classification assessment due to inherent signal interference from hemorrhage and proteinaceous content, potentially obscuring enhancement visibility.

Purpose: To investigate whether MR subtraction imaging improves interobserver agreement and diagnostic performance in the Bosniak classification of T1-hyperintense CRMs.

Study Type: Retrospective.

Papillary Renal Neoplasm With Reverse Polarity: CT and MR Imaging Characteristics in 26 Patients.

Rationale And Objectives: To improve the diagnostic recognition of papillary renal neoplasm with reverse polarity (PRNRP) through comprehensive analysis of computed tomography (CT) and magnetic resonance imaging (MRI) findings.

Materials And Methods: A retrospective multi-center study was conducted on patients with pathologically confirmed PRNRPs from 2019 to 2024, encompassing six institutions. Clinical and pathological data were meticulously documented.

Constructing ultra-stable, high-energy, and flexible aqueous zinc-ion batteries using environment-friendly organic cathodes.

Due to their sustainability, environmental friendliness, high specific capacity, and rapid reaction kinetics, quinone cathodes have broad application prospects in aqueous zinc-ion batteries (AZIBs). However, conventional small-molecule quinone cathodes usually suffer from unavoidable dissolution, resulting in terrible cycling stability. Herein, based on a strategy of molecular structure optimization, calix[8]quinone (C8Q) is for the first time used as a cathode in AZIBs.

Cross-modality integration framework with prediction, perception and discrimination for video anomaly detection.

Video anomaly detection is an important task for public security in the multimedia field. It aims to distinguish events that deviate from normal patterns. As important semantic representation, the textual information can effectively perceive different contents for anomaly detection.

Improving the Thickness Uniformity of Micro Gear by Multi-Step, Self-Aligned Lithography and Electroforming.

The thickness nonuniformity of an electroformed layer is a bottleneck problem for electroformed micro metal devices. In this paper, a new fabrication method is proposed to improve the thickness uniformity of micro gear, which is the key element of various microdevices. The effect of the thickness of the photoresist on the uniformity was studied by simulation analysis, which showed that as the thickness of the photoresist increased, the thickness nonuniformity of the electroformed gear should decrease due to the reduced edge effect of the current density.

Discovery of semisynthetic celastrol derivatives exhibiting potent anti-ovarian cancer stem cell activity and STAT3 inhibition.

The hallmark of ovarian cancer is its high mortality rate attributed to the existence of cancer stem cells (CSCs) subpopulations which result in therapy recurrence and metastasis. A series of C-29-substituted and/or different A/B ring of celastrol derivatives were synthesized and displayed potential inhibition against ovarian cancer cells SKOV3, A2780 and OVCAR3. Among them, compound 6c exhibited the most potent anti-proliferative activity and selectivity, gave superior anti-CSC effects through inhibition of the sphere formation and downregulation of the percentage of CD44CD24 and ALDH cells.

Facile preparation of hierarchical micro-nano FeF·0.33HO by a one-pot method with dual surfactants.

To prepare a hierarchical micro-nano structure FeF·0.33HO simply and economically, a one-pot method with dual surfactants was used. Scanning electron microscopy and a Fourier transformation infrared spectrometer revealed that polyvinyl pyrrolidone (PVP) regulates the morphology of the material, while cetyltrimethylammonium bromide (CTAB) can reshape FF@PVP, it can not only remove PVP at room temperature, but also obtain a hierarchical micro-nano structure.

Ultrabroadband Photodetectors up to 10.6 µm Based on 2D Fe O Nanosheets.

The ultrabroadband spectrum detection from ultraviolet (UV) to long-wavelength infrared (LWIR) is promising for diversified optoelectronic applications of imaging, sensing, and communication. However, the current LWIR-detecting devices suffer from low photoresponsivity, high cost, and cryogenic environment. Herein, a high-performance ultrabroadband photodetector is demonstrated with detecting range from UV to LWIR based on air-stable nonlayered ultrathin Fe O nanosheets synthesized via a space-confined chemical vapor deposition (CVD) method.

Large-Scale Ultrathin 2D Wide-Bandgap BiOBr Nanoflakes for Gate-Controlled Deep-Ultraviolet Phototransistors.

Ternary two-dimensional (2D) semiconductors with controllable wide bandgap, high ultraviolet (UV) absorption coefficient, and critical tuning freedom degree of stoichiometry variation have a great application prospect for UV detection. However, as-reported ternary 2D semiconductors often possess a bandgap below 3.0 eV, which must be further enlarged to achieve comprehensively improved UV, especially deep-UV (DUV), detection capacity.

Self-Confined Growth of Ultrathin 2D Nonlayered Wide-Bandgap Semiconductor CuBr Flakes.

2D planar structures of nonlayered wide-bandgap semiconductors enable distinguished electronic properties, desirable short wavelength emission, and facile construction of 2D heterojunction without lattice match. However, the growth of ultrathin 2D nonlayered materials is limited by their strong covalent bonded nature. Herein, the synthesis of ultrathin 2D nonlayered CuBr nanosheets with a thickness of about 0.

A Microfabricated 96-Well 3D Assay Enabling High-Throughput Quantification of Cellular Invasion Capabilities.

This paper presents a 96-well microfabricated assay to study three-dimensional (3D) invasion of tumor cells. A 3D cluster of tumor cells was first generated within each well by seeding cells onto a micro-patterned surface consisting of a central fibronectin-coated area that promotes cellular attachment, surrounded by a poly ethylene glycol (PEG) coated area that is resistant to cellular attachment. Following the formation of the 3D cell clusters, a 3D collagen extracellular matrix was formed in each well by thermal-triggered gelation.

Optimization of extracting stachyose from Stachys floridana Schuttl. ex Benth by response surface methodology.

Stachys floridana schuttl. E x benth, also named yinmiao, is a special and traditional Chinese vegetable that is usually used by some diabetes patients as a pharmaceutical plant for its high content of stachyose. Due to the lower cost and higher economic reward, Stachys floridana schuttl.

Clinical values of urinary IL-6 in asymptomatic renal hematuria and renal hematuria with proteins.

Renal hematuria is caused by glomerular disease. Under pathological conditions, the distribution of interleukin-6 (IL-6) in kidney tissue is abnormal and urinary IL-6 levels are increased. Abnormal IL-6 secretion promotes the hyperplasia of mesangial cells and matrix and, thus, affects the permeability of the glomerular filtration membrane.

[Isolation and characterisation of an aflatoxin B1-degrading bacterium].

Objective: Aflatoxins are toxic and carcinogenic fungal metabolites. The aim of this research was to isolate aflatoxin B1 (AFB1)-degrading bacteria.

Methods: Samples from various sources were screened by using coumarin as the sole carbon source.

High-precision thickness regulation of graphene layers with low energy helium plasma implantation.

In this paper we present a novel method of regulation to obtain graphene layers with homogeneous thickness by means of helium plasma implantation. The obtained graphene layers show neither large deep pits nor loss of lateral dimension. The etching rate can be precisely controlled (one to six atomic layers min(-1) or higher) and it remains consistent regardless of the thickness of the multilayer graphene.

[Analysis of differential expression proteins and functions in Lactobacillus brevis NCL912 under acid stress].

Object: We investigated the proteomic profile of Lactobacillus brevis NCL912 under optimum pH and acidic pH in the media without the addition of sodium L- glutamate to characterize the differential expression proteins and function by two-dimensional gel electrophoresis.

Methods: The differential expression proteins were separated and analyzed by two-dimensional gel electrophoresis, mass spectrum and bioinformatics.

Results: The results showed that the two-dimensional gel electrophoresis profiles of L.

Proteomic analysis of differentially expressed proteins in Lactobacillus brevis NCL912 under acid stress.

Protein expression of Lactobacillus brevis NCL912 under acid stress was analysed by two-dimensional gel electrophoresis and MS. Twenty-five proteins were differentially expressed under acid stress. Among them, eight protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight MS, of which seven were upregulated and one was downregulated.

Number of blastomeres and distribution of microvilli in cloned mouse embryos during compaction.

The events resulting in compaction have an important influence on the processes related to blastocyst formation. To analyse the quality of the embryos obtained by somatic cell nuclear transfer (SCNT) in aspects different from previous studies, not only the number of blastomeres of cloned embryos during the initiation of compaction, but also the distribution of microvilli in cloned, normal, parthenogenetic, and tetraploid embryos before and after compaction was preliminarily investigated in mouse. Our results showed that during compaction the number of blastomeres in SCNT embryos was fewer than that in intracytoplasmic sperm injection (ICSI) embryos and, before compaction, there was a uniform distribution of microvilli over the blastomere surface, but microvilli became restricted to an apical region after compaction in the four types of embryos.

Uncooled infrared imaging device based on optimized optomechanical micro-cantilever array.

It is a major issue to improve the thermo-mechanical sensitivity of uncooled optomechanical focal plane arrays (FPAs) for infrared imaging. This work presents an optimized multi-fold interval metallized leg (IML) configuration to increase the thermo-mechanical sensitivity of an uncooled optomechanical bi-material micro-cantilever array. The inclination angle changes of the cantilever elements are measured in the IR imaging system using an optical readout with a knife-edge filtering operation in the spectrum plane.

Uncooled IR imaging using optomechanical detectors.

In this study, we present an uncooled infrared imaging detector using knife-edge filter optical readout method. The tilt angle change of each cantilever in a focal plane array (FPA) can be simultaneously detected with a resolution of 10(-5) degrees. A deformation magnifying substrate-free microcantilever unit is specially designed.