Draft genome sequence of the obligate bacterial endosymbiont of the aphid .

The genome of the obligate bacterial endosymbiont from the aphid was sequenced and assembled using Illumina paired-end sequencing. Genomic functional analysis revealed that can assist its host in counteracting oxidative stress induced by plant-derived sesquiterpene lactones.

Tailored therapy guided by genotypic resistance from gastric mucosa samples in the first-line treatment of infection: a systematic review and meta-analysis.

Background: Tailored therapy is crucial for reducing the irrational use of antibiotics and enhancing () eradication rates. Detection of antibiotic resistance gene mutations offers a rapid, accurate, and simple approach for tailored therapy, facilitating its widespread clinical implementation. However, the efficacy, safety, adherence, and cost-effectiveness of tailored therapy guided by genotypic resistance from gastric mucosa samples for first-line treatment remain unclear.

Complete genome sequence of the obligate endosymbiont of the poplar bark aphid .

We report the complete genome sequence of from the aphid using Illumina NovaSeq X Plus platform. The assembled genome is 588,150 bp with a guanine-cytosine content of 24%.

The Systemic Impact of Helicobacter pylori Infection on the Microbiome of Whole Digestive Tract Based on Mucosal, Gastric Juice, and Fecal Specimens.

Background: Recent studies have found that in addition to directly impacting the gastric microbiome, Helicobacter pylori (H. pylori) infection may cause intestinal microbial dysbiosis. However, most existing studies on the influence of H.

Comparative analysis of the detection of antibiotic genotypic resistance with gastric mucosa, gastric fluid, and fecal samples in patients with infection.

Unlabelled: Genotypic methods for detecting antibiotic resistance in infection offer a rapid, convenient, and accurate approach for tailored therapy. However, existing studies predominantly examine single sample types and lack comparative analyses across different samples. This study comprehensively detects and compares genotypic resistance to clarithromycin and levofloxacin in gastric mucosa, gastric fluid, and fecal samples from the same patients.

The complete mitochondrial genome of (Takahashi, 1919) (Hemiptera: Aphididae: Chaitophorinae).

We sequenced the complete mitochondrial genome of the aphid It is 16,828 bp long and includes 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosome RNA genes (rRNAs), a control region, and a repeat region. The control region contains a 273-bp repeat motif repeated 2.3 times.

Effect of neoadjuvant chemoradiotherapy with or without PD-1 antibody sintilimab in pMMR locally advanced rectal cancer: A randomized clinical trial.

Neoadjuvant chemoradiotherapy (NACRT) was the standard treatment for patients with locally advanced rectal cancer (LARC) with proficient mismatch repair (pMMR) proteins. In this randomized phase 2 trial (ClinicalTrial.gov: NCT04304209), 134 pMMR LARC patients were randomly (1:1) assigned to receive NACRT or NACRT and the programmed cell death protein 1 (PD-1) antibody sintilimab.

Characteristics and Comparative Analysis of Mitochondrial Genomes of the Aphid Genus Koch (Hemiptera: Aphididae: Aphidinae).

Using Illumina sequencing technology, we generated complete mitochondrial genomes (mitogenomes) of three constituent species of the aphid genus Koch, (Blanchard), Ghulamullah, and (Geoffroy). The sizes of the mitogenomes range from 15,306 to 15,410 bp, primarily due to variations in the length of non-coding regions. The mitogenomes consist of 37 coding genes arranged in the order of the ancestral insect mitogenome, a control region, and a repeat region between and According to the COI-based analysis, one previously reported mitogenome of should be assigned to .

Minocycline in the eradication of infection: A systematic review and meta-analysis.

Background: Difficulty in obtaining tetracycline, increased adverse reactions, and relatively complicated medication methods have limited the clinical application of the classic bismuth quadruple therapy. Therefore, the search for new alternative drugs has become one of the research hotspots. In recent years, minocycline, as a semisynthetic tetracycline, has demonstrated good potential for eradicating () infection, but the systematic evaluation of its role remains lacking.

Toluidine blue O photosensitizer combined with caffeic acid improves antibacterial performance by increasing the permeability of cell membrane.

Photodynamic therapy has always been an antibacterial tool for solving multi-drug resistant bacteria problem, but the side effects and the low efficiency due to the high aggregation and low solubility of photosensitizers limit its application. Due to the anti-inflammatory effect of caffeic acid, two novel photosensitizers (CA-1-TBO, CA-TBO) were synthesized by conjugating caffeic acid with toluidine blue O (TBO). The structures have been characterized by HNMR and high-resolution mass spectrometry.

GDF15 Interference Regulates Proliferation, Inflammation, and Autophagy of Lipopolysaccharide-induced Mesangial Cells by Inhibiting PI3K/ AKT/mTOR Signaling.

Background: Chronic glomerulonephritis (CGN) is a primary glomerular disease. As a circulating protein, growth and differentiation factor 15 (GDF15) participates in a variety of biological processes.

Objective: We aimed to investigate the role of GDF15 in CGN.

Sophorolipid-toluidine blue conjugates for improved antibacterial photodynamic therapy through high accumulation.

Anti-bacterial photodynamic therapy is the most promising treatment protocol for bacterial infection, but low accumulation of photosensitizers has seriously hindered their development in clinical application. Here, with inherent outstanding affinity to bacterial cell envelope, sophorolipid produced from has been conjugated to toluidine blue (SL-TB) through amidation reaction. The structure of SL-TB conjugates was identified by H-NMR, FT-IR and ESI-HRMS.

Bismuth, esomeprazole, metronidazole, and minocycline or tetracycline as a first-line regimen for Helicobacter pylori eradication: A randomized controlled trial.

Background: Given the general unavailability, common adverse effects, and complicated administration of tetracycline, the clinical application of classic bismuth quadruple therapy (BQT) is greatly limited. Whether minocycline can replace tetracycline for Helicobacter pylori ( H . pylori ) eradication is unknown.

Individualized diagnosis and eradication therapy for Helicobacter pylori infection based on gene detection of clarithromycin resistance in stool specimens: A systematic review and meta-analysis.

Background: Empiric therapy for Helicobacter pylori infection results in significantly increased antibiotic resistance and decreased eradication efficacy. The genotypic testing of clarithromycin resistance from stool specimens is a promising method for individualized diagnosis and treatment. This study aimed to determine the status of research and application on this method through a systematic review and meta-analysis.

New regimens as first-line eradication therapy for Helicobacter pylori infection in patients allergic to penicillin: A randomized controlled trial.

Background: Helicobacter pylori eradication in penicillin-allergic patients is challenging. The effective regimen is lacking in areas with high antibiotic resistance and tetracycline unavailable. Minocycline, cefuroxime, and full-dose metronidazole are promising drugs.

Outer membrane protein of OmpF contributes to swimming motility, biofilm formation, osmotic response as well as the transcription of maltose metabolic genes in Citrobacter werkmanii.

Bacterial outer membrane proteins (Omps) are essential for environmental sensing, stress responses, and substance transport. Our previous study discovered that OmpA contributes to planktonic growth, biocide resistance, biofilm formation, and swimming motility in Citrobacter werkmanii, whereas the molecular functions of OmpF in this strain are largely unknown. Thus, in this study, the ompF gene was firstly knocked out from the genome of C.

Bismuth, esomeprazole, metronidazole and amoxicillin or tetracycline as a first-line regimen for Helicobacter pylori eradication: A randomized controlled trial.

Background: Due to general unavailability and common side effects of tetracycline, the clinical application of bismuth quadruple therapy (BQT) is greatly limited. Whether amoxicillin can replace tetracycline in BQT remains unknown. This study aimed to compare the eradication rate, safety and compliance between amoxicillin-containing and tetracycline-containing BQT as a first-line regimen for Helicobacter pylori eradication.

ompX contribute to biofilm formation, osmotic response and swimming motility in Citrobacter werkmanii.

Citrobacter werkmanii, an aerobe and mesophilic Proteobacterium, is universal in industrial putrefaction, coastal water, and human blood. Our previous studies have discovered that outer membrane protein X (OmpX) of C. werkmanii is involved in calcium response, but the underlying mechanisms and its molecular characteristics remain elusive.

The complete mitochondrial genome of Zhang, 1982 (Hemiptera: Aphididae: Chaitophorinae).

We assembled and annotated the complete mitochondrial genome of , which is the first complete mitogenome in subfamily Chaitophorinae. The circular mitogenome of is 16,148 bp long with an A + T content of 84.1%.

CD147 deficiency is associated with impairedsperm motility/acrosome reaction and offersa therapeutic target for asthenozoospermia.

Patients with asthenozoospermia often present multiple defects in sperm functions apart from a decrease in sperm motility. However, the etiological factors underlying these multifaceted defects remain mostly unexplored, which may lead to unnecessary treatment and unsatisfactory assisted reproductive technologies (ART) outcome. Here, we show that the protein levels of CD147 were lowered in sperm obtained from asthenozoospermic infertile patients exhibiting defects in both sperm motility and the acrosome reaction.

The complete mitochondrial genome of van der Goot, 1917 (Hemiptera: Aphididae: Hormaphidinae).

In this study, the complete mitochondrial genome of was sequenced and annotated. The mitogenome is 14,990 bp in length with an A + T content of 82.6%, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and a control region.

PPI-amoxicillin dual therapy four times daily is superior to guidelines recommended regimens in the Helicobacter pylori eradication therapy within Asia: A systematic review and meta-analysis.

Background: Systematic reviews suggested that the eradication efficacy of PPI-amoxicillin dual therapy is similar to that of other commonly used regimens. However, it might be affected by the medication frequency. Basic and clinical studies have shown that dual therapy administered four-times daily has a reliable pathophysiological basis and could achieve satisfactory efficacy.

A series of carboxymethyl cellulose-based antimicrobial peptide mimics were synthesized for antimicrobial applications.

Inspired by antimicrobial peptides (AMP) which could alleviate drug resistance pressure, antimicrobial peptide mimics (AMPMs) were designed timely. Here, carboxymethyl cellulose (CMC) -based AMPMs were constructed by introducing different diamines on CMC effectively. Firstly, CMC was degraded to be oligomers with different molecular weights, followed by amination reactions with different diamines respectively.

Complete mitochondrial genome of (Okajima, 1908) (Hemiptera: Aphididae: Greenideinae).

Here we report the complete mitochondrial genome of the aphid species . The mitogenome is 15,727 bp in length and comprising 37 genes typically present in insect mitogenomes, a control region, and a unique repeat region. All protein-coding genes (PCGs) terminate with TAA or TAG except for , which is terminated with T-.

The complete mitochondrial genome of (Okajima, 1908) (Hemiptera: Aphididae: Greenideinae).

In this study, we sequenced the complete mitochondrial genome of through Illumina platform. The circular mitogenome is 16,500 bp in length and composed of 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), a large control region and a special repeat region. The nucleotide composition of whole mitogenome is strongly AT-biased (85.