Preliminary Study on Rapid and Simultaneous Detection of Viable O157:H7, s, and by PMA-mPCR in Food.

O157:H7, , and are major foodborne pathogens that are widespread in nature and responsible for several outbreaks of food safety accidents. Thus, a rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable O157:H7, , and in pure culture and in a food matrix. To eliminate false positive results, propidium monoazide (PMA) was applied to selectively suppress the DNA amplification of dead cells.

Research on three-state reliability evaluation method of high reliability system based on multi-source prior information.

A high reliability system has the characteristics of complexity, modularization, high cost and small sample size. Throughout the entire lifecycle of system development, storage and use, the high reliability requirements and the risk analysis form a direct contradiction with the testing expenses. In order to ensure the system, module or component maintains good reliability status and effectively reduces the cost of sampling tests, it is necessary to make full use of multi-source prior information to evaluate its reliability.

Chinese Few-Shot Named Entity Recognition and Knowledge Graph Construction in Managed Pressure Drilling Domain.

Managed pressure drilling (MPD) is the most effective means to ensure drilling safety, and MPD is able to avoid further deterioration of complex working conditions through precise control of the wellhead back pressure. The key to the success of MPD is the well control strategy, which currently relies heavily on manual experience, hindering the automation and intelligence process of well control. In response to this issue, an MPD knowledge graph is constructed in this paper that extracts knowledge from published papers and drilling reports to guide well control.

Underlying mechanisms and molecular targets of genistein in the management of type 2 diabetes mellitus and related complications.

Diabetes mellitus (DM) is a chronic metabolic disease caused by a complex interaction of genetic and environmental factors and is characterized by persistent hyperglycemia. Long-term hyperglycemia can cause macrovascular and microvascular damage, and compromise the heart, brain, kidney, peripheral nerves, eyes and other organs, leading to serious complications. Genistein, a phytoestrogen derived from soybean, is known for its various biological activities and therapeutic properties.

Screening eco-friendliness tire antioxidants alternatives: functional 2,2,4-trimethyl-1,2-dihydroquinoline derivatives design and toxicity evaluation.

Antioxidants which are indispensable functional additives used in rubber tires, are released into aquatic habitats from tire wear particles (TWP), collected in water bodies, and threaten the aquatic ecosystem. This study aimed to design eco-friendly derivatives of 2,2,4-trimethyl-1,2-dihydroquinoline (TMQ) with increased antioxidant activity to use as tire antioxidants. Initially, seventy highly efficient derivatives of TMQ were designed by hydroxylation modifications at multiple sites.

Right, but not left, posterior superior temporal gyrus is causally involved in vocal feedback control.

The posterior superior temporal gyrus (pSTG) has been implicated in the integration of auditory feedback and motor system for controlling vocal production. However, the question as to whether and how the pSTG is causally involved in vocal feedback control is currently unclear. To this end, the present study selectively stimulated the left or right pSTG with continuous theta burst stimulation (c-TBS) in healthy participants, then used event-related potentials to investigate neurobehavioral changes in response to altered auditory feedback during vocal pitch regulation.

Identification of Robust Antibiotic Subgroups by Integrating Multi-Species Drug-Drug Interactions.

Previous studies have shown that antibiotics can be divided into groups, and drug-drug interactions (DDI) depend on their groups. However, these studies focused on a specific bacteria strain (i.e.

Cervical cell nuclei segmentation based on GC-UNet.

Cervical cancer diagnosis hinges significantly on precise nuclei segmentation at early stages, which however, remains largely elusive due to challenges such as overlapping cells and blurred nuclei boundaries. This paper presents a novel deep neural network (DNN), the Global Context UNet (GC-UNet), designed to adeptly handle intricate environments and deliver accurate cell segmentation. At the core of GC-UNet is DenseNet, which serves as the backbone, encoding cell images and capitalizing on pre-existing knowledge.

Detection of Single Non-small Cell Lung Cancer Cell Multidrug Resistance with Single-Cell Bioanalyzer.

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death in the world. Despite the development of various lung cancer treatment methods, including surgery, radiation therapy, endocrine therapy, immunotherapy, and gene therapy, chemotherapy remains the most common approach for treating cancer. The risk of tumors acquiring resistance to chemotherapy remains a significant hurdle to the use of this approach for the successful treatment of various types of cancer.

Detection of Single Cancer Cell Multidrug Resistance With Single Cell Bioanalyzer.

Despite the development of various cancer treatment methods, chemotherapy remains the most common approach for treating cancer. The risk of tumors acquiring resistance to chemotherapy remains a significant hurdle to the successful treatment of various types of cancer. Therefore, overcoming or predicting multidrug resistance in clinical treatment is essential.

Optimizing the Production of Antioxidant and Antibacterial Alkaloids from Macleaya cordata by Subcritical Water Extraction Technology.

In this work, the subcritical water extraction technology was used to extract alkaloids from Macleaya cordata, and the effects of extraction temperature and time on its yield were investigated to find the best extraction conditions. Moreover, the antioxidant capacity and antibacterial activity of Macleaya cordata extract were studied. Furthermore, through the single-factor method, it was found that properly increasing the extraction temperature and prolonging the extraction time was conducive to increasing alkaloid yield.

CdO decorated CdS nanorod for enhanced photocatalytic reduction of CO to CO.

Solar-driven CO reduction into fuels and sustainable energy has attracted increasing attention around the world. However, the photoreduction efficiency remains low due to the low efficiency of separation of electron-hole pairs and high thermal stability of CO. In this work, we prepared a CdO decorated CdS nanorod for visible light driven CO reduction.

Targeted teaching method combined with stepped assessment in public tennis lessons.

In order to put students in the spotlight and make it simpler for them to understand and master the teaching topics, public tennis classes in colleges and universities used a combination of targeted teaching techniques and a staged evaluation method. A random sampling was used to select 200 students from public physical education classes at Zhuhai University of Science and Technology as the study population. They were divided into a control group and an experimental group of 100 students each (50 male and 50 female).

Potential thyroid hormone disorder risks of tire antioxidants to aquatic food chain organisms after absorbing free radicals in marine and freshwater environments.

Tire antioxidants are essential functional chemical additives in tire rubber production. Because of the characteristic easy precipitation in the water environment, the environmental pollution problem caused by tire antioxidants is concerning. To reveal the mechanism by which tire antioxidants reduce common oxidative factors (free radicals) in the environment and to control the potential biological thyroid hormone disorder risk of tire antioxidant derivatives, eight commonly used antioxidants in tire production were selected for analysis.

Energy differences as descriptors for the correlation between and in nonfullerene organic photovoltaics.

ITIC-series nonfullerene organic photovoltaics (NF OPVs) have realized the simultaneous increases of the short-circuit current density () and open-circuit voltage (), called the positive correlation between and , which could improve the power conversion efficiency (PCE). However, it is complicated to predict the formation of positive correlation in devices through simple calculations of single molecules due to their dimensional differences. Here, a series of symmetrical NF acceptors blended with the PBDB-T donor were chosen to establish an association framework between the molecular modification strategy and positive correlation.

LFDNN: A Novel Hybrid Recommendation Model Based on DeepFM and LightGBM.

Hybrid recommendation algorithms perform well in improving the accuracy of recommendation systems. However, in specific applications, they still cannot reach the requirements of the recommendation target due to the gap between the design of the algorithms and data characteristics. In this paper, in order to learn higher-order feature interactions more efficiently and to distinguish the importance of different feature interactions better on the prediction results of recommendation algorithms, we propose a light and FM deep neural network (LFDNN), a hybrid recommendation model including four modules.

Work as a Frontline Volunteer During the COVID-19 Outbreak in Hubei, China: A Qualitative Inquiry of Male Nurses.

Background: During the coronavirus (COVID-19) outbreak in 2019, an increased large number of male nurses volunteered for frontline assignment. Their excellent performance suggests that male nurses have several advantages over female nurses. However, research into the activities of Chinese male nurses engaged in frontline work during the COVID-19 pandemic remains limited.

Experiences and perspectives of healthcare professionals implementing advance care planning for people suffering from life-limiting illness: a systematic review and meta-synthesis of qualitative studies.

Background: Life-limited patients may lose decision-making abilities during disease progression. Advance care planning can be used as a discussion method for healthcare professionals to understand patients' future care preferences. However, due to many difficulties, the participation rate of healthcare professionals in advance care planning is not high.

Brain-targeted ginkgolide B-modified carbonized polymer dots for alleviating cerebral ischemia reperfusion injury.

Ischemic stroke (IS) is a leading cause of death in the world, and there is still a lack of effective treatments. Ginkgolide B (GB) can antagonize the platelet activating factor receptor and has shown a significant curative effect on cerebral ischemia. However, GB and other drugs for IS have shown poor clinical efficacy due to their inability to cross the blood-brain barrier (BBB).

Exploring the Potential Hormonal Effects of Tire Polymers (TPs) on Different Species Based on a Theoretical Computational Approach.

Tire polymers (TPs) are the most prevalent type of microplastics and are of great concern due to their potential environmental risks. This study aims to determine the toxicity of TPs with the help of molecular-dynamics simulations of their interactions with receptors and to highlight the differences in the toxicity characteristics of TPs in different environmental media (marine environment, freshwater environment, soil environment). For this purpose, five TPs-natural rubber, styrene-butadiene rubber (SBR), butadiene rubber, nitrile-butadiene rubber, and isobutylene-isoprene rubber-were analyzed.

A challenge of deep-learning-based object detection for hair follicle dataset.

Background: Deep-learning object detection has been applied in various industries, including healthcare, to address hair loss.

Methods: In this paper, YOLOv5 object detection algorithm was used to detect hair follicles in a small and specific image dataset collected using a specialized camera on the scalp of individuals with different ages, regions, and genders. The performance of YOLOv5 was compared with other popular object detection models.

Development of a food preservative from sea buckthorn together with chitosan: Application in and characterization of fresh-cut lettuce storage.

The purpose was to create a novel composite food preservative for fresh-cut lettuce using flavonoids and chitosan from sea buckthorn leaves (SBL). Sea buckthorn leaves were extracted with ethanol as the extraction solvent and ultrasonic-assisted extraction to obtain flavonoid from sea buckthorn leaf crude (FSL), and then the FSL was secondarily purified with AB-8 resin and polyamide resin to obtain flavonoid from sea buckthorn leaf purified (FSL-1). Different concentrations of FSL-1 and chitosan were made into a composite preservative (FCCP) by magnetic stirring and other methods, containing 1% chitosan preservative (CP) alone, 0.

Dietary supplementation of extract alleviates motor deficits in MPTP-induced Parkinson's disease mice and its underlying mechanism.

extract (ASE), a dietary supplement with antifatigue, neuroprotective, and immunomodulatory properties, has been widely used due to its high polyphenol content. Our previous study showed that ASE could be used to treat Parkinson's disease (PD) as it contains multiple monoamine oxidase B inhibitors prescribed in early PD. However, its mechanism remains ambiguous.