Influence of Age on Fracture Healing in Young and Middle-Aged Mice in a Proximal Femur Fracture Model.

Osteoporotic hip fractures are a considerable cause of pain and disability particularly among the elderly. Osteoporosis causes loss of bone stability, which in turn leads to an increased risk of fractures especially in metaphyseal bone. Moreover, the body's capacity for healing is diminished, resulting in prolonged recovery times following these fractures.

L-Carnitine Modulates Oocyte Development and Cumulus Expansion in Tropical Ruminants: Experimental and Bioinformatics Insights.

This study investigates the effects of L-carnitine on nuclear maturation and fertilization in cattle and goat oocytes. Ovaries were collected from females with poor reproductive efficiency in the tropical climate, and cumulus-oocyte complexes (COCs) were retrieved from large antral follicles. COCs were cultured with varying concentrations of L-carnitine (0, 0.

Accelerating patient transitions from hospital to home in the United Kingdom with single-use negative pressure wound therapy: a discharge pathway.

Background: Initially limited to inpatient use, negative pressure wound therapy (NPWT) is now frequently used in community settings. However, complexities in wound management step-down strategies in the United Kingdom, including regional variations in referral processes, lack of consensus on funding criteria, and limited availability of NPWT units, have led to extended hospital length of stay (LOS) for patients ready for discharge but still needing NPWT. Single-use NPWT (sNPWT) can serve as a bridge between hospital and community NPWT.

Efficacy of cellular and/or tissue-based product applications on all non-pressure injury chronic wound types in a Medicare private practice model.

Background: This retrospective analysis is a derivative cohort study based on a prior retrospective investigation by this author group.

Objective: To assess the effect of the number of cellular and/or tissue-based product (CTP) applications on healing outcomes and wound area reduction (WAR) rates in patients with chronic wounds of multiple etiologies.

Methods: Data from a multicenter private wound care practice electronic health record database were analyzed for Medicare patients receiving CTPs from January 2018 through December 2023.

The trial design of the concurrent optical and magnetic stimulation (COMS) therapy study for refractory diabetic foot ulcers (MAVERICKS): a multicenter, randomized, sham-controlled, double-blind investigational device exemption clinical study.

Background: Diabetic foot ulcers (DFUs) are a major clinical challenge, particularly among patients with refractory ulcers, that often lead to severe complications such as infection, amputation, and high mortality. Innovations supported by strong clinical evidence have the potential to improve healing outcomes, enhance quality of life, and reduce the economic burden on individuals and health care systems.

Objective: To describe the design of the concurrent optical and magnetic stimulation (COMS) therapy Investigational Device Exemption (IDE) study for refractory DFUs (MAVERICKS) trial.

Preoperatively predicting failure to achieve the minimum clinically important difference and substantial clinical benefit for total knee arthroplasty patients using machine learning.

Background: A clear understanding of minimal clinically important difference (MCID) and substantial clinical benefit (SCB) is essential for effectively implementing patient-reported outcome measurements (PROMs) as a performance measure for total knee arthroplasty (TKA). Since not achieving MCID and SCB may reflect suboptimal surgical benefit, the primary aim of this study was to use machine learning to predict patients who may not achieve the threshold-based outcomes (i.e.

Reimagining Outcomes: A Perspective Review of Advances in Remote Monitoring Technologies in Post-Arthroplasty Patient Care.

Arthroplasty surgery is a common and successful end-stage intervention for advanced osteoarthritis. Yet, postoperative outcomes vary significantly among patients, leading to a plethora of measures and associated measurement approaches to monitor patient outcomes. Traditional approaches rely heavily on patient-reported outcome measures (PROMs), which are widely used, but often lack sensitivity to detect function changes (e.

TCA cycle-derived oncometabolites in cancer and the immune microenvironment.

Oncometabolites are aberrant metabolic byproducts that arise from mutations in enzymes of the tricarboxylic acid (TCA) cycle or related metabolic pathways and play central roles in tumor progression and immune evasion. Among these, 2-hydroxyglutarate (2-HG), succinate, and fumarate are the most well-characterized, acting as competitive inhibitors of α-ketoglutarate-dependent dioxygenases to alter DNA and histone methylation, cellular differentiation, and hypoxia signaling. More recently, itaconate, an immunometabolite predominantly produced by activated macrophages, has been recognized for its dual roles in modulating inflammation and tumor immunity.

High-resolution time-series transcriptomic and metabolomic profiling reveals the regulatory mechanism underlying salt tolerance in maize.

Background: Soil salinization represents a critical global challenge to agricultural productivity, profoundly impacting crop yields and threatening food security. Plant salt-responsive is complex and dynamic, making it challenging to fully elucidate salt tolerance mechanism and leading to gaps in our understanding of how plants adapt to and mitigate salt stress.

Results: Here, we conduct high-resolution time-series transcriptomic and metabolomic profiling of the extremely salt-tolerant maize inbred line, HLZY, and the salt-sensitive elite line, JI853.

Tutorial: Saturation Transfer Difference NMR for Studying Small Molecules Interacting With Nanoparticles.

Saturation transfer difference (STD) NMR is a robust, versatile technique for detecting small molecules binding to large receptors. In addition to identifying binding molecules in the presence of nonbinding molecules, the STD-NMR technique can be used to determine epitope maps and binding constants. In recent years, this technique has been applied to small molecules interacting with nanoparticles.

Monitoring Gait Recovery After Total Knee Arthroplasty Using Wearable Sensors: Responsiveness of Gait Accelerations.

Functional recovery after total knee arthroplasty (TKA) varies widely among individuals, and traditional assessments often fail to detect subtle changes in real-world walking ability. Wearable sensors offer continuous and objective tracking of gait outside of clinical settings. In this prospective, longitudinal study, thirty-one patients undergoing unilateral TKA wore thigh-mounted accelerometers continuously from 2 weeks before surgery through 90 days postoperatively.

LONP1 Variants Are Associated With Clinically Diverse Phenotypes.

LONP1 encodes a mitochondrial protease essential for protein quality control and metabolism. Variants in LONP1 are associated with a diverse and expanding spectrum of disorders, including Cerebral, Ocular, Dental, Auricular, and Skeletal anomalies syndrome (CODAS), congenital diaphragmatic hernia (CDH), and neurodevelopmental disorders (NDD), with some individuals exhibiting features of mitochondrial encephalopathy. We report 16 novel LONP1 variants identified in 16 individuals (11 with NDD, 5 with CDH), further expanding the clinical spectrum.

Characterizing Fidelity Monitoring Practices in Community Behavioral Health Care.

Evidence-based practices (EBPs) are most effective when they are delivered with a high degree of fidelity, or as they are intended to be delivered. Because clinicians often deviate from fidelity, it is important to monitor EBP fidelity over time to guide corrective actions. However, little is known about current fidelity monitoring practices in community behavioral health care.

Prism Model: Factors that Influence Teaming in Behavioral Health from the Perspectives of Interprofessional Clinicians.

This qualitative study explores what factors influence teaming in behavioral health settings, from the perspective of behavioral health providers. Twenty-four participants from a range of behavioral health professions engaged in semi-structured interviews. Using a grounded theory approach, data were analyzed, and a "prism" model was developed to capture the complexities of behavioral health providers' perceptions of factors influencing teaming in various mental health and/or substance use disorder treatment programs.

Impact of Flow Restrictors on Aerosol Delivery of the Respimat® Soft Mist Inhaler.

Introduction: The modification of an inhaler's air flow resistance influences a patient's inhalation flow profile, thereby affecting the exit velocity of an aerosol leaving the Respimat® mouthpiece. A slower inhalation maneuver results in reduced plume velocity and thus a decreased oropharyngeal deposition due to reduced impaction. This could not only lead to fewer unwanted side effects associated with inhaled therapies, but also enhance lung deposition.

A Promising Frontier in Cancer Therapy - Combining Endothelial Colony Forming Cells, Nanotechnology, and Hyperthermia for Precision Oncology.

Endothelial Colony-Forming Cells (ECFCs) are recognized as key vasculogenic progenitors in humans and serve as valuable liquid biopsies for diagnosing and studying vascular disorders. In a groundbreaking study, Anceschi et al. present a novel, integrative strategy that combines ECFCs loaded with gold nanorods (AuNRs) to enhance tumor radiosensitization through localized hyperthermia.

ASO Practice Guidelines Series: Soft Tissue Sarcoma of the Extremities and Superficial Trunk.

Soft tissue sarcomas (STS) are a heterogeneous group of rare malignant tumors arising from mesenchymal tissues, with extremity and superficial trunk STS (eSTS) comprising the majority of cases. The management of localized eSTS requires a multidisciplinary approach to optimize oncologic and functional outcomes. This review outlines the natural history, diagnostic workup, and treatment principles for localized eSTS, emphasizing the role of histology-specific considerations in guiding management strategies.

Evaluation of efficacy and safety of sequential antibody drug conjugates (ADCs) in human epidermal growth factor 2 (HER2)-negative metastatic breast cancer.

Purpose: Limited data is available assessing sequencing of antibody drug conjugates (ADCs) in patients with hormone receptor-positive (HR +), human epidermal growth factor 2 (HER2)-negative, HER2-low, and triple-negative metastatic breast cancer (MBC), including patients with brain metastases (BrM) or leptomeningeal disease (LMD). This study assesses the efficacy and safety of sequential sacituzumab govitecan (SG) and trastuzumab deruxtecan (T-DXd) in MBC and impact on chemotherapy (CTX).

Methods: This is a single-center, retrospective, cohort study in adult patients with HR + , HER2-negative, or low MBC who received T-DXd and/or SG.

A novel fluorescent aptasensor using magnetic silica nanospheres and Ag@MOF nanocomposites for extraction and detection of Salmonella Typhimurium in eggs.

Salmonella Typhimurium (S. Typhimurium) is one of the most common food-borne diseases, highlighted as the top food-borne bacterial pathogen in the world with a low infectious dose (1 CFU) and high mortality rate. It is commonly associated with numerous foods such as dairy products, protein sources (multiple types of meat, poultry, and eggs), and bakery products.

The impact of melatonin-enriched media on epigenetic and perinatal changes induced by embryo culture in a mouse model.

Purpose: To determine if melatonin-enriched culture media could offset loss of imprinting in mouse concepti.

Methods: Zygotes were cultured to blastocyst stage under optimized conditions in melatonin-supplemented media at either 10 M (MT 10) or 10 M (MT 10), or without supplementation (Culture + embryo transfer, or ET, positive control). Blastocysts were also developed in vivo (ET negative control).

The value of optical genomic mapping in a patient with azoospermia and a complex chromosomal rearrangement.

Introduction: Complex chromosomal rearrangements (CCRs) are frequently associated with infertility and have been described in the literature. Chromoanagenesis corresponds to a group of CCRs with a high number of chromosome breakpoints. These CCRs involving small structural variations can only be identified by using high-resolution genomic techniques.