Beneficial Effects of the Mitochondria-Targeted Antioxidant MitoQ on Bull Semen Post Cryopreservation Quality Characteristics.

This experiment evaluated the effects of increasing MitoQ concentrations in semen extender on post-thaw quality of Holstein bull sperm, including motility, membrane integrity, antioxidant status and viability. Semen samples were collected, pooled and diluted with extender containing 0 (control), 5, 50, 500 and 1000 nM of MitoQ and frozen through the standard procedure. An increase in MitoQ supplementation positively influenced total and progressive motility, as well as average path velocity; however, these effects were not statistically significant until the concentration reached 50 nM.

An integrated approach to optimizing environmental impact and crop yields in the regenerative agriculture system of Central Europe.

Achieving sustainable agriculture requires balancing high productivity with minimal environmental impact, a challenge that is ongoing for both farmers and policymakers. This study proposes an innovative approach by integrating life cycle assessment (LCA), data envelopment analysis (DEA), causal loop diagrams (CLD), and the DPSIR (Driving forces, Pressures, State, Impacts, Responses) framework to evaluating regenerative agriculture systems (RAS) in Central Europe. Focusing on wheat and maize production in Slovakia, we assessed scenarios involving different herbicides containing glyphosate and pyridine compounds, as well as the use of cow manure.

Variations in conventional and non-conventional semen characteristics of selected rabbit breeds.

This article strove to characterize and compare biochemical, oxidative, bacteriological and immunological semen properties in Zemplin, Liptov Baldspotted and New Zealand rabbits. Besides, bacteria were characterized using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oxidative profile of semen was assessed using chemiluminescent and colorimetric protocols.

Evaluation of the Profile of Selected Bioactive Compounds and the Potential of Barley Wort Enriched with Malted and Unmalted Hemp Seeds for Brewing Applications.

The incorporation of Cannabis sativa L. seeds into barley wort was investigated to enhance the functional profile of beer. Hemp seeds (cv.

Proteomics coupled machine learning-innovative approach in geographical origin authentication of green Coffea arabica.

The geographical authentication of green specialty coffee is an economically sensitive analytical task that is not yet fully resolved. We used an innovative combination of proteomic profiling with linear discriminant analysis for the authentication of the geographical origin of green specialty coffee beans from well-known harvesting regions in Central America, South America, Africa, and Asia. Out of 1596 identified proteins, we selected the top 30 target markers ranked by ANOVA.

The therapeutic potential of cyanidin-3-O-glucoside relating to female reproductive health.

Cyanidin-3-O-glucoside (C3G), a dietary flavonoid found in berries, exhibits strong antioxidant, anti-inflammatory, and anticancer properties. It plays a role in female reproductive health by protecting ovarian cells from oxidative stress while inhibiting tumour growth and inducing apoptosis in ovarian and cervical cancer cells. C3G can modulate estrogen receptors, growth factors, and apoptosis- and angiogenesis-related pathways.

Generation of Novel Monoclonal Antibodies Recognizing Rabbit CD34 Antigen.

The rabbit is a widely used experimental model for human translational research and stem cell therapy. Many studies have focused on rabbit mesenchymal stem cells from different biological sources for their possible application in regenerative medicine. However, a minimal number of studies have been published aimed at rabbit hematopoietic stem/progenitor cells, mainly due to the lack of specific anti-rabbit CD34 antibodies.

Elemental uptake and accumulation by wood decay fungi in relation to their nutrition strategy, rot type and mineral profile of the colonized wood.

The uptake and accumulation of essential and toxic elements by wood decay fungi (WDF) are complex. We examined the mineral profile of 13 WDF colonizing coniferous and deciduous trees. To illustrate the role of nutritional strategies and rot type in selected elements uptake and accumulation, four fungi species differing in their nutrition strategy and the type of decay, colonizing only conifers were selected: two white rot: saprotrophic Pseudohydnum gelatinosum, and parasitic Phellinus pini and two brown rot, saprotrophic/parasitic Phaeolus schweinitzii and saprotrophic Tapinella atrotomentosa.

Oryzalin-Induced polyploidy enhances natural metabolite accumulation and functional bioactivities in novel Callisia fragrans genotype.

The reorientation of Callisia fragrans (Lindl.) Woodson, from therapeutic to ornamental use, exemplifies a broader domestication trend, favoring aesthetics over medicinal properties. Renewed phytomedicinal interest and the rise of plant extract markets drive demand for organic, sustainable consumer products, heightening the competition for superior cultivars.

The Effect of Freeze-Dried Cherry Pomace and Red Potato Pulp on the Content of Bioactive Substances in Pasta.

Pasta, due to its convenience, follows bread as the most common cereal product in the human diet. Typical wheat pasta is a high-energy product, since it contains a large amount of starch; at the same time, it is characterized by a low content of health-promoting ingredients, such as dietary fiber, minerals, vitamins, and polyphenols. Food industry by-products, or even waste, can be applied as a source of many bioactive substances, thus enriching pasta with bioactive ingredients.

Predictive Modelling and Optimisation of Rubber Blend Mixing Using a General Regression Neural Network.

This paper presents an intelligent predictive system designed to support real-time decision making in the control of rubber blend mixing processes. The core of the system is a General Regression Neural Network (GRNN), which accurately predicts key process parameters, such as viscosity (expressed as torque), temperature, and energy consumption across varying masses of the processed material. The model can evaluate the mixing progress based on the initial 10% of input data, allowing early intervention and process optimisation.

Effectiveness of a Remotely Delivered Whole Food Plant-Based Diet Intervention With Online Community Support: Pilot Study.

Community-based interventions using an Whole Food Plant-Based Diet (WFPB) effectively promote weight loss and reduce cardiovascular risk factors. Although in-person delivery fosters valuable social interaction, it also increases costs and limits scalability. This pilot study evaluated a 5-week, remotely delivered WFPB intervention with online community support among 18 women with overweight or obesity, 16 of who completed outcome measures.

Somatic cell count and presence of microbial pathogens in milk of goats in Slovakia.

The objectives of the study were to determine somatic cell count (SCC) and evaluate the presence of pathogens (IMI - intramammary infection) in late lactation (LL), followed by the start (colostrum, CL) and approximate peak (established lactation, EL) of the next lactation, as well as to assess the possible transmission of IMI from lactation to lactation. The study was performed on a dairy farm in northern Slovakia. A total of 489 half udder milk samples (242, 80 and 167 in LL, CL and EL, respectively) were collected.

Polyphenolic spectrum of cornelian cherry fruits and their health-promoting effect.

Although cornelian cherry is an underutilized fruit species, its fruits have a high biological value due to valuable biologically active substances, especially polyphenols. The total content of polyphenols accounts for 37% of all the bioactive substances examined. Flavonols, anthocyanins, flavan-3-ols, and phenolic acids represent the main groups of phenolic compounds present and thanks to these compounds, cornelian cherry fruits possess mainly antioxidant, anti-inflammatory, antimicrobial, metabolic, antidiabetic, cardioprotective, and anticancer effects.

Age-related changes in semen characteristics of selected Slovak geese breeds.

This paper strove to characterize and compare ejaculates collected from two geese breeds over the period of two years in the context of selected traditional, biochemical, oxidative, bacteriological, and immunological properties. Slovak white and Suchovy ganders were selected for sperm quality evaluation, while bacteria were characterized using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oxidative profile of semen was assessed using chemiluminescent and colorimetric protocols.

Can Bisphenols Alter the Inflammation Process?

This review's main purpose is to draw attention to the possible influence of widely used bisphenols on the inflammatory process. Bisphenols are endocrine-disrupting chemicals that are produced worldwide in great quantities. From this point of view, it is very important to clarify their influence on innate immune reactions, which protect the integrity of the body against the action of various pathogens on a daily basis.

Toxic elements and fatty acid composition in the freshwater fish family Cyprinidae (Rafinesque 1815): balancing nutritional benefits and health risks.

The aim of this study was to assess the toxicity of heavy metals/metalloids, including arsenic, cadmium, lead, and mercury accumulated in the muscle of commonly consumed fish from the Cyprinidae. We discussed the importance of fatty acids in the human diet and investigated their profile in the muscle of different fish species. Additionally, our goal was to evaluate the benefits of fish consumption in relation to its risks, not only by considering the advantages of fatty acids and the drawbacks of heavy metal toxicity but also by examining how these pollutants may alter the fatty acid profile in fish muscle, potentially reducing the quality of their nutritional benefits.

Post-Curing Effects on the Tensile Properties of Hybrid Fiber-Reinforced Polymers: Experimental and Numerical Insights.

This study investigates the effects of post-curing temperatures on the tensile properties of hybrid basalt-jute-glass-carbon fiber-reinforced polymers (FRPs). Composite specimens were post-cured at 60 °C and 100 °C for 60 min, and their tensile behavior was assessed using a servo-hydraulic testing machine. Numerical simulations using the Abaqus software V6.

Gender-based differences in eco-efficient farming.

This study investigates gender-based differences in eco-efficiency among Hungarian field crop farms, using data from the Farm Accountancy Data Network between 2015 and 2020. Applying Data Envelopment Analysis and Blinder-Oaxaca decompositions, we reveal a consistent eco-efficiency advantage for women-headed farms, particularly at mid-quantiles of eco-efficiency distribution. This advantage is largely attributed to women's ability to optimize resource use effectively, though unexplained factors also contribute, suggesting potential differences in management practices.

Repurposing major metabolites of lamiaceae family as potential inhibitors of -synuclein aggregation to alleviate neurodegenerative diseases: an approach.

Neurodegenerative disorders (NDs) are typically characterized by progressive loss of neuronal function and the deposition of misfolded proteins in the brain and peripheral organs. They are molecularly classified based on the specific proteins involved, underscoring the critical role of protein-processing systems in their pathogenesis. Alpha-synuclein (α-syn) is a neural protein that is crucial in initiating and progressing various NDs by directly or indirectly regulating other ND-associated proteins.

Effects of Feeding Poultry Slaughterhouse Byproduct Meal on the Growth Performance, Digestibility, Antioxidant Status and Histological Parameters of Broilers.

A total of 3000 1-day-old chicks were randomly assigned to 5 groups with 6 replicates (n = 100). The control group was fed standard diets, whereas the other groups were fed diets containing poultry slaughterhouse meal (PSM) at levels of 50, 75, 100 or 125 kg/t. The results indicated that the group fed 75 kg/t of PSM exhibited greater body weight (BW) and body weight gain (BWG) than the other groups.

The effect of metformin and sea buckthorn (Hippophae rhamnoides L.) in alleviating the symptoms of type 2 diabetes mellitus in the liver of zucker diabetic fatty rats.

Sea buckthorn (Hippophae rhamnoides L.) has many beneficial health properties. In our previous study we showed suppression of hyperglycaemia, water intake, decreased sorbitol levels in the lens of the eyes after sea buckthorn m(Hippophae rhamnoides L.

Changes in the Intracellular Composition of Macro and Microminerals After Cryopreservation of the Rabbit Stem/Progenitor Cells.

Cryopreservation is a widely used method for the long-term preservation of reproductive or somatic cells. It is known that this storage method may negatively affect cell viability, proliferation, differentiation, etc. However, there is a lack of information about whether cryostorage can alter the content of intracellular minerals.

Phytochemical Screening and Monitoring of Intercellular Changes in Murine Leydig Cells After the Treatment of Trigonella foenum-graecum L. Microgreens In Vitro.

The objective of our in vitro study was to quantify the biochemical profile where the total polyphenol, flavonoid and phenolic acid content was determined. The antioxidant potential of microgreen extract from Trigonella foenum-graecum L., was measured molybdenum reducing power assay.