Reducing allergenicity of Trachinotus ovatus parvalbumin: Insights into digestibility and IgE-binding ability after dense phase CO₂ treatment.

Parvalbumin (PV), a thermostable and digestion-resistant fish allergen, has been shown to retain its allergenic potential following traditional treatments, thus posing a persistent allergic risk. The study investigated the digestive kinetics and IgE immunoreactivity of Trachinotus ovatus PV, a major fish allergen, under different treatments (untreated; DPCD treatment-15 MPa, 30 min, 50 °C; heat treatment), to evaluate its allergenic potential alterations. The analysis was conducted using a combination of techniques to assess the proteolytic stability and IgE-binding capacity of PV, including Tris-Tricine-SDS-PAGE, Western blot (WB), indirect enzyme-linked immunosorbent assay (ELISA), and free amino group quantification.

Bi-allelic deleterious variants in SNAPIN, which encodes a retrograde dynein adaptor, cause a prenatal-onset neurodevelopmental disorder.

Fetal brain anomalies identified by prenatal ultrasound and/or magnetic resonance imaging represent a considerable healthcare burden with ∼1-2/1,000 live births. To identify the underlying etiology, trio prenatal exome sequencing or genome sequencing (ES/GS) has emerged as a comprehensive diagnostic paradigm with a reported diagnostic rate up to ∼32%. Here, we report five unrelated families with six affected individuals that presented neuroanatomical, craniofacial, and skeletal anomalies, all harboring rare, bi-allelic deleterious variants in SNAPIN, which encodes SNARE-associated protein.

Bacterial infections and antimicrobial resistance patterns: a comprehensive analysis of health dynamics across regions in Pakistan (2013-2023).

Antimicrobial resistance (AMR) is a significant public health concern globally, and Pakistan is no exception. The misuse and overuse of antibiotics, inadequate regulation of their sale, and a lack of awareness contribute to the rising levels of AMR in the country. study presents a detailed analysis of blood and urine samples collected in Pakistan over various periods, focusing on pathogen prevalence, gender distribution, and age-wise patterns.

Evidence for Similar Collectivity of High Transverse-Momentum Particles in p-Pb and Pb-Pb Collisions.

Charged hadron elliptic anisotropies (v_{2}) are presented over a wide transverse momentum (p_{T}) range for proton-lead (pPb) and lead-lead (PbPb) collisions at nucleon-nucleon center-of-mass energies of 8.16 and 5.02 TeV, respectively.

Elemental analysis of molybdenum ore using charge coupled device (CCD) and intensified charge-coupled device (ICCD) based LIBS systems.

Time-resolved data acquisition is crucial for compositional analysis using Laser-Induced Breakdown Spectroscopy (LIBS). It can be managed by adjusting the delay time and gate width of the spectrometer. This study describes the compositional analysis of molybdenum (Mo) ore utilizing charge coupled device (CCD) and intensified charge-coupled device (ICCD) based LIBS systems.

Therapeutic Power of Date Fruit ( L.): A Nutrient-Rich Superfood for Holistic Health and Disease Prevention.

Date fruit ( L.) is a highly nutritious and therapeutic food with substantial potential to improve human health. This review emphasizes the nutritional and therapeutic traits of date fruit, focusing on its role as a functional food and dietary supplement.

Exploring the In Vitro Anti-Inflammatory Effect of Citrus Fruit Hesperidin Supplementation.

The aim of the present study was to assess the anti-inflammatory effect of hesperidin. The research was conducted by optimizing the hesperidin extraction process from citrus peel powder, followed by characterization and nutrition profiling of citrus peel hesperidin extract. Citrus peel was collected from the local market and dried in a hot air oven.

Effects of chicken manure-derived black soldier fly organic fertilizer on soil carbon and nitrogen cycling: insights from metagenomic and microbial network analysis.

Black soldier fly (BSF) organic fertilizer is known to enhance soil fertility and promote plant growth. However, its effects on soil carbon (C) and nitrogen (N) cycling remains unclear. In this study, we established a BSF chicken manure bioconversion system to produce BSF organic fertilizer and investigate its impacts on soil C and N cycling, as well as microbial ecological networks through metagenomic analysis.

Recent Advances in Traceability, Authentication, and Bioactive Profiling of Fruit and Fruit-Based Products Using Analytical Techniques Combined With Chemometrics.

Fruit and fruit-based products are a valuable source of essential nutrients, critical for food security, and drive economic productivity with minimal inputs. The significant rise in global demand for high-quality imported fruit and fruit-based products reflects a shift in consumer awareness and interest in the products origin and potential health-promoting bioactive compounds. Analytical techniques such as liquid chromatography, gas chromatography, inductively coupled plasma techniques, isotope-ratio mass spectrometry (IRMS), near infrared (NIR) spectroscopy, visible near infrared (VIS-NIR) spectroscopy, hyperspectral imaging (HSI), mid-infrared (MIR) spectroscopy, Raman spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fluorescence spectroscopy, terahertz spectroscopy, dielectric spectroscopy, electronic nose (e-nose), and electronic tongue (e-tongue) coupled with supervised and unsupervised chemometrics can be employed for traceability, authentication, and bioactive profiling of fruit and fruit-based products.

Inter-modality feature prediction through multimodal fusion for 3D shape defect detection.

3D shape defect detection plays an important role in autonomous industrial inspection. However, accurate detection of anomalies remains challenging due to the complexity of multimodal sensor data, especially when both color and structural information are required. In this work, we propose a lightweight inter-modality feature prediction framework that effectively utilizes multimodal fused features from the inputs of RGB, depth and point clouds for efficient 3D shape defect detection.

variants and rheumatoid arthritis susceptibility in women from Central Mexico.

Rheumatoid arthritis (RA) is a chronic autoimmune disease in which dysregulated interferon regulatory factor 5 (IRF5) may amplify pro-inflammatory pathways; prior genetic studies of IRF5 single-nucleotide variants (SNVs) in RA are inconsistent across populations and have not included mestizo Mexicans or evaluated rs59110799 in RA. We aimed to test whether four IRF5 SNVs (rs2004640G/T, rs2070197T/C, rs10954213G/A, rs59110799G/T) confer susceptibility to RA in women from Central Mexico. In a case-control study of 239 women with RA and 231 female controls (all self-identified Mexican-Mestizos, ≥3 generations), genotyping was performed by real-time PCR with TaqMan® probes; 80% of samples were duplicated (100% concordance) and control genotypes conformed to Hardy-Weinberg equilibrium.

Transforming Waste to Innovation: Sustainable Piezoelectric Properties of Pb(Ti Zr )O with Recycled β‑PbO Massicot.

In this study, we present an indigenous approach to enhancing the properties of Pb-(ZrTi)-O by synthesizing it from β-PbO obtained from spent lead-acid batteries. Initially, β-PbO, orthorhombic massicot, was produced by two-step heating, and 99.9% lead powder was derived from recovered lead-acid batteries at 700 °C.

Morphology and multi-locus phylogeny reveal four new (Psathyrellaceae) species from Pakistan.

In this study, four new species of the genus are described from Pakistan. Based on morphological and multi-locus phylogenetic analyses, we confirmed the distinction of these taxa. The newly identified species are designated as , , , and .

Noninvasive multiclass milk contaminants detection using hyperspectral imaging and hybrid ensemble learning.

Food contamination remains a serious global concern due to its health risks, with milk being one of the most commonly adulterated foods in developing countries such as Pakistan, India, and Bangladesh. Accurate detection of milk contamination is essential for ensuring consumer safety and maintaining food industry standards. This study explores both invasive and noninvasive approaches for contamination analysis.

Metal-Organic Framework (MOF)-Based Catalysts for Sustainable Energy Technologies: A Review.

The demand for sustainable energy technologies is high due to the depletion and risks linked to fossil fuel usage. Diverse energy technologies, such as regenerative fuel cells, zinc-air batteries, and comprehensive water-splitting devices, possess significant potential for the advancement of green energy. MOFs hold a prominent position among the various kinds of materials utilized in renewable energy technologies.

AI in conjunctivitis research: assessing ChatGPT and DeepSeek for etiology, intervention, and citation integrity via hallucination rate analysis.

Introduction: The advent of large language models and their applications have gained significant attention due to their strengths in natural language processing.

Methods: In this study, ChatGPT and DeepSeek are utilized as AI models to assist in diagnosis based on the responses generated to clinical questions. Furthermore, ChatGPT, Claude, and DeepSeek are used to analyze images to assess their potential diagnostic capabilities, applying the various sensitivity analyses described.

Cultural narratives, social norms, and psychological stigma: a study of mental health help-seeking behavior in Peshawar, Pakistan.

Introduction: Mental health stigma remains a major barrier to accessing psychiatric care worldwide, with pronounced effects in culturally traditional societies such as Peshawar, Pakistan. In the Pashtun cultural context, the code of Pashtunwali-an honor-based system-shapes social attitudes and behaviors, potentially influencing mental health help-seeking patterns. This study examines how cultural narratives, social norms, and stigma interact to affect help-seeking behavior in this sociocultural setting.

Excellent hydrogen storage capabilities and optoelectronic attributes of XClH (Li, Na, and K) perovskite hydrides for green energy technologies.

The high hydrogen storage capacity, stability, and reversibility of perovskite hydrides make them promising materials for the energy industry. They play a vital role in sustainable energy technologies, including fuel cells and hydrogen storage systems. This work offers a comprehensive understanding of the physical attributes of XClH (X = Li, Na, and K), utilizing the DFT-based Wien2K code.

Platinum/nitrogen-co-doped TiO as photocatalyst and light-free catalytic adsorbent for gaseous formaldehyde.

Platinum and nitrogen co-doped titanium dioxide (Pt/N-TiO, with 1 wt% Pt and an N/Ti molar ratio of 1) has been synthesized. This Pt/N co-doping strategy creates Schottky junctions, reduces the bandgap energy (3.25 to 2.

Heavy metals toxicity in plants: understanding mechanisms and developing coping strategies for remediation: a review.

Heavy metal (HM) contamination is an increasing environmental and agricultural concern due to the persistence, toxicity, and bioaccumulative nature of metals such as cadmium (Cd), lead (Pb), mercury (Hg), and arsenic (As). These pollutants are primarily introduced through industrial effluents, mining, and agrochemicals, negatively impacting soil health, crop productivity, and food safety, ultimately posing serious risks to both ecosystems and human health. Conventional remediation methods can be costly, labor-intensive, and environmentally disruptive.

Exome sequencing in a Pakistani male infertility cohort: perspective on molecular diagnosis, genetic heterogeneity, and diagnostic yield.

Male infertility affects a significant number of couples worldwide, yet the precise causes and genetic mechanisms underlying this condition remain largely unknown. To investigate the monogenic causes of primary male infertility, we performed exome sequencing (ES) in a cohort of 100 unrelated Pakistani male patients with non-obstructive, non-syndromic primary infertility. ES identified potential causal variants in established infertility-associated genes in 17/100 patients, resulting in a diagnostic yield of 17%.

Hopscotching perovskites: DFT leveraged tight-binding insights into inorganic lead halide perovskites.

Delocalization insights into inorganic lead halide perovskites of the form CsPbBrI ( = 0, 1, 2, 3), obtained a DFT based tight-binding method, are presented. Compared to first principles studies like DFT (physically accurate and computationally expensive), the tight-binding approach allows the disentanglement of the region of interest, namely, the Fermi level. Further adjustment of the hopping norm and maximum distance leads to a simplified, highly interpretable but chemically grounded, reduced model which regenerates the broad features of the band structure with a fraction of the parameters.

Facile preparation of cobalt-oxide nanostructures with enriched cobalt(ii) ion surface using for energy application.

The increasing demand for efficient energy conversion and storage systems necessitates the development of high-performance, cost-effective electrode materials. To address this challenge, we employed rotten (eggplant) juice as a precursor for the fabrication of low-cost, earth-abundant, and active electrode materials based on cobalt oxide (CoO) nanostructures. Different volumes of rotten juice (5 mL, 10 mL, 15 mL, and 20 mL) were utilized during the precipitation process to synthesize CoO nanostructures.