Understanding Health Information Systems Utilization Across Public Health Centers in Indonesia: Cross-Sectional Study.

Background: The primary health care service in Indonesia consists of 10,260 public health centers (Puskesmas), which play a major role in providing health care in the community, recording and reporting health data using digital health information systems (HIS) or manual reports. The utilization of HIS across Puskesmas is crucial to capture the dynamic evolution of health problems and monitor interventions, thus providing effective primary health care services for the community.

Objective: This paper provides a national-level baseline mapping of HIS utilization in Indonesian Puskesmas.

Procollagen IIA mediates positive feedback control of the mouse cardiogenic transcriptional network.

Cardiogenesis relies on the integrated interplay between cardiac transcription factors and signaling pathways. Here, we uncover a role for type IIA procollagen (IIA), an extracellular matrix (ECM) protein encoded by an alternatively spliced transcript, encoding a N-terminal cysteine-rich domain, as a critical regulator in a cardiac gene regulatory feedback loop. The cysteine-rich domain of IIA protein was previously reported to interact with bone morphogenetic proteins (BMPs) and transforming growth factors-beta (TGFβ) in in vitro binding assays and acts as a BMP antagonist in amphibian embryo assays.

Evaluation of whole blood CD64 for identifying infection in neonates receiving hospital care.

Introduction: Infection remains one of the most common causes of death in neonates. However, early detection of neonatal infections to inform treatment decisions remains clinically and technically challenging due to the non-specific nature of symptoms, and the lack of a sufficiently accurate diagnostic test. Neonatal infections and sepsis in adults have been associated with increased CD64 expression on neutrophils.

Functional Profiling Demonstrates That a Sulfide-Reducing Diet Achieves Microenvironmental Targets in Ulcerative Colitis.

Background: As a dietary approach to reducing inflammation in ulcerative colitis, the 4-SURE (4 Strategies to Sulfide Reduction) diet was designed to correct pathogenic alterations of excessive protein fermentation and hydrogen sulfide (H2S) production in the distal colon. We aimed to perform a deep functional analysis (microbial and metabolomic) of the feces of 28 adults with mild-moderately active ulcerative colitis who adhered to the 4-SURE diet over 8 weeks to explore whether the 4-SURE diet could modulate the intraluminal environment as intended.

Methods: Fecal samples were collected at week 0 and 8 of dietary intervention, processed and aliquoted.

'You've Made These Choices…These Are the Consequences'. General Nurses' Perspectives on Caring for Young People With Eating Disorders: A Qualitative Descriptive Study.

Eating disorders are a group of conditions characterised by an alteration in patterns of food intake and have a high risk of mortality. Eating disorders are particularly challenging to treat because they encompass both mental and physical domains of healthcare, requiring a complex multidisciplinary approach that often involves clinicians who may only have singular training: Medical or mental health. The aim of this study was to describe the experiences and perspectives of general paediatric nurses caring for young people with medically compromised eating disorders in a general paediatric setting.

Measuring Australian Primary School Children's Water Safety Knowledge: Alignment With the Victorian Water Safety Certificate.

Background: Water safety education is essential to prevent drowning. In Australia, while water safety education is included in the school curriculum, the focus is often on skill attainment rather than knowledge.

Methods: This study assessed water safety knowledge in Grade 5 and 6 students (aged 9-11 years).

Single-Cell Fluorescence Imaging Reveals Heterogeneity in Senescence Biomarkers and Identifies Rapamycin-Responsive Sub-Populations.

Cellular senescence is a state of irreversible cell cycle arrest accompanied by a distinctive inflammatory secretory profile known as the senescence-associated secretory phenotype (SASP). While various biomarkers, such as senescence-associated beta-galactosidase (SA-βgal), EdU incorporation, p21 and p16, are used to identify senescent cells, no single biomarker universally defines cellular senescence and current methods often fail to address heterogeneity in biomarker expression levels. This study leverages single-cell fluorescence imaging to assess multiple senescence markers including SA-βgal enzymatic activity, p21 and IL-6 expression and nuclear and cell area in chemotherapy-induced (mitomycin C) and oxidative stress-induced (D-galactose) senescence models in human fibroblasts.

Lead Astray? The Hidden Contaminants in Australian Anabolic-Androgenic Steroid Market and Their Potential Health Impact.

Introduction: Unregulated anabolic-androgenic steroid (AAS) markets are a global phenomenon with significant variability in product composition and purity. This study aimed to determine the chemical composition of AAS sold in Australia.

Methods: This study analysed anonymously donated AAS samples.

Do Child Gender Stereotypes Mediate Associations Between Human Fathers' Testosterone Levels and Paternal Involvement? Two Studies.

This preregistered study investigated whether human paternal testosterone levels predicted fathers' time spent with their child and their involvement in childcare and household tasks. Additionally, we examined whether associations were mediated by fathers' attitudes toward child gender stereotypes. Associations were tested in an exploratory sample (n = 70, M = 6.

Screening for perinatal depression in Australia: Validation of the Edinburgh Postnatal Depression Scale in pregnancy and the postpartum.

Background: Universal screening of all perinatal women using the Edinburgh Postnatal Depression Scale is currently recommended in Australian National Guidelines, yet Australian validation studies of this measure are limited and with mixed findings. This study aims to address a current gap using the largest Australian sample to include both antenatal and postpartum periods to evaluate the performance screening for Major Depression.

Method: Data from 887 women is drawn from the Mercy Pregnancy and Emotional Wellbeing Study, a prospective cohort, in Melbourne, Perth and regional and rural Western Australia.

Recombinant human hyaluronidase-facilitated subcutaneous immunoglobulin (hf-SCIg) for inflammatory myositis: a multicenter retrospective real-world observational study.

Background: Subcutaneous immunoglobulin (SCIg) is a promising alternative to intravenous Ig (IVIg) for the treatment of idiopathic inflammatory myositis (IIM), thanks to its more favorable safety profile, reduced costs, and lower impact on patients' quality of life. We assessed the short- and long-term effectiveness and safety of recombinant human hyaluronidase-facilitated SCIg (hf-SCIg) in patients with IIM treated at different referral centers in Italy.

Methods: A multicenter, retrospective, real-life cohort study was conducted on consecutive adult patients diagnosed with IIM according to the EULAR/ACR criteria, treated with hf-SCIg for remission induction or maintenance.

Social prescribing for informal carers: a pre-post study.

Background: Informal carers often experience compromised mental and physical wellbeing due to their caring responsibilities. While social prescribing shows promise in supporting various populations, evidence for its effectiveness in supporting carers is limited. This study assessed the impact of a social prescribing program on health-related quality of life and other wellbeing measures among Australian adult carers.

Genome-wide CRISPR screen identifies Menin and SUZ12 as regulators of human developmental timing.

Embryonic development follows a conserved sequence of events across species, yet the pace of development is highly variable and particularly slow in humans. Species-specific developmental timing is largely recapitulated in stem cell models, suggesting a cell-intrinsic clock. Here we use directed differentiation of human embryonic stem cells into neuroectoderm to perform a whole-genome CRISPR-Cas9 knockout screen and show that the epigenetic factors Menin and SUZ12 modulate the speed of PAX6 expression during neural differentiation.

Projecting Global Trends and Inequalities in Adult Overweight and Obesity, 2023-2040: Findings From the NCD-RisC Database.

Objective: This study quantifies the global burden of overweight and obesity, projects future trends, and examines associated health inequalities.

Methods: Overweight and obesity burden data were obtained from the NCD-RisC database. Trends from 1990 to 2022 were analyzed, and a Bayesian model was used to project changes for 2023-2040.

Paternal prepubertal passive smoke exposure is related to impaired lung function trajectories from childhood to middle age in their offspring.

Introduction: Paternal prepubertal passive smoke exposure may increase the risk of childhood asthma. However, its association with impaired lung function trajectories at risk of chronic obstructive pulmonary disease in offspring was not investigated. We assessed the association between paternal prepubertal passive smoke exposure and lung function from childhood to middle age in their offspring.

ROS-induced modifications of fibrin clots connect immune responses to atherothrombosis in systemic lupus erythematosus.

Objective: Cardiovascular events are major determinants of morbidity and mortality in systemic lupus erythematosus (SLE), particularly in patients with renal involvement. While oxidative stress has been implicated in driving vascular and renal damage in SLE, the specific mechanisms remain unclear. This study investigated the potential role of oxidative stress-induced alterations in fibrinogen structure and function in the pathogenesis of atherothrombosis in SLE.

Variation in temperature but not diet determines the stability of latitudinal clines in tolerance traits and their plasticity.

Latitudinal clines are routinely used as evidence of adaptation across broad climatic gradients. However, if environmental variation influences the strength of latitudinal clines, then clinal patterns will be unstable, and using patterns of adaptation to predict population responses to global change will be difficult. To test whether environmental variation influences latitudinal clines, we sampled five populations of spanning 3000 km of east coast Australia, and measured stress tolerance (heat, cold and desiccation) and body size on flies that developed in six combinations of temperature (13°C, 25°C and 29°C) and diet (standard and low-calorie) treatments.

Code to complex: AI-driven de novo binder design.

The application of artificial intelligence to structural biology has transformed protein design from a conceptual challenge into a practical approach for creating new-to-nature proteins. By leveraging machine learning, researchers can now computationally design proteins with tailored architectures and binding specificities. This has enabled the rapid in silico generation of high-affinity binders to diverse and previously intractable targets.

Comparative venom proteomes of Malaysian snake species from regions in Southeast Asia obtained by shotgun proteomics: A systematic review.

Snakebite envenoming (SBE) is a critical yet neglected public health issue in ASEAN countries, including Malaysia. Antivenom immunotherapy is the primary treatment for SBE, functioning by neutralising toxic venom components through antibodies raised from immunised animals. Current antivenoms come with potential reduced efficacy due to regional venom variability.