Adoptive transfer of third-party donor CMV-specific T cells for refractory cytomegalovirus infection following umbilical cord blood transplantation.

Refractory cytomegalovirus (CMV) infection is a severe complication following umbilical cord blood transplantation (UCBT). Antiviral agents, the standard first-line therapy, are limited by toxicity and resistance without robust T-cell immunity. We evaluated third-party donor (TPD)-derived CMV-specific T cells (CMVSTs) as a treatment option.

Estimands for Early-Phase Dose Optimization Trials in Oncology.

Phase I dose escalation trials in oncology generally aim to find the maximum tolerated dose. However, with the advent of molecular-targeted therapies and antibody drug conjugates, dose-limiting toxicities are less frequently observed, giving rise to the concept of optimal biological dose (OBD), which considers both efficacy and toxicity. The estimand framework presented in the addendum of the ICH E9(R1) guidelines strengthens the dialogue between different stakeholders by bringing in greater clarity in the clinical trial objectives and by providing alignment between the targeted estimand under consideration and the statistical analysis methods.

Use of a novel zipper device for wound closure of cutaneous abscesses in pediatric outpatients: a case series.

Background: Current management of pediatric cutaneous abscesses involves either spontaneous healing by secondary intention or suturing through tertiary intention, which are often lengthy processes that cause discomfort and distress among children. As it is noninvasive and simple, a novel zipper device is widely used for the primary wound closure of surgical incisions.

Objective: To describe the effectiveness of novel zipper device use for pediatric cutaneous abscess wound closure in an outpatient context.

The trial design of the concurrent optical and magnetic stimulation (COMS) therapy study for refractory diabetic foot ulcers (MAVERICKS): a multicenter, randomized, sham-controlled, double-blind investigational device exemption clinical study.

Background: Diabetic foot ulcers (DFUs) are a major clinical challenge, particularly among patients with refractory ulcers, that often lead to severe complications such as infection, amputation, and high mortality. Innovations supported by strong clinical evidence have the potential to improve healing outcomes, enhance quality of life, and reduce the economic burden on individuals and health care systems.

Objective: To describe the design of the concurrent optical and magnetic stimulation (COMS) therapy Investigational Device Exemption (IDE) study for refractory DFUs (MAVERICKS) trial.

Preoperatively predicting failure to achieve the minimum clinically important difference and substantial clinical benefit for total knee arthroplasty patients using machine learning.

Background: A clear understanding of minimal clinically important difference (MCID) and substantial clinical benefit (SCB) is essential for effectively implementing patient-reported outcome measurements (PROMs) as a performance measure for total knee arthroplasty (TKA). Since not achieving MCID and SCB may reflect suboptimal surgical benefit, the primary aim of this study was to use machine learning to predict patients who may not achieve the threshold-based outcomes (i.e.

Use of the i-PARIHS framework in nutrition and dietetics research and practice: A citation analysis of the literature and case studies.

Theoretical approaches can help to plan, guide, and evaluate implementation projects that target real-world practice problems. This paper provides an overview of the integrated Promoting Action on Research Implementation in Health Services (i-PARIHS) framework and summarizes its use in nutrition and dietetics research and practice. A narrative summary of its use was compiled from the published literature based on citations from two key reference sources of the i-PARIHS framework.

Implications of Changing the Diagnostic Criteria for Gestational Diabetes Mellitus (CDC4G): A Healthcare Cost Analysis Alongside a Stepped Wedge Cluster Randomised Trial.

Objective: To estimate the effect on healthcare resource use after introducing the World Health Organization diagnostic criteria (WHO-2013) for gestational diabetes mellitus (GDM) compared to former criteria in Sweden (SWE-GDM).

Design: A cost-analysis alongside the Changing Diagnostic Criteria for Gestational Diabetes (CDC4G) randomised controlled trial.

Setting: Sweden, with risk-factor based screening for GDM.

Bridging the knowledge gap: a mixed-methods study on general practitioners' information needs for mHealth apps in hypertension treatment in Germany.

Background: Hypertension remains a critical public health issue in Germany, affecting millions of individuals. Mobile health applications (mHealth apps) offer promising solutions for improving patient outcomes and adherence in hypertension management. Despite their advantages in healthcare, the adoption of mHealth apps by general practitioners (GPs) in Germany remains limited to date.

Professional experiences with journal club implementation in postgraduate nursing: a qualitative study.

Background: Postgraduate education is embracing journal clubs (JCs), which provide a platform for members to critically evaluate research articles and extract evidence-based nursing practice. The implementation of JCs by postgraduate nurses, especially in varied educational contexts such as Egypt, remains underexplored. This study aimed to explore and gain valuable insights into the professional experiences of implementing JCs among postgraduate nursing students in Egypt.

A Sensitive and Comprehensive LC-MS/MS Method for the Analysis of Hallucinogens, Synthetic Cathinones, and Synthetic Cannabinoids in Urine Samples.

The laboratory analysis of new psychoactive substances and related drugs is crucial for accurate clinical and forensic diagnosis of poisonings. Given this, a new LC-MS/MS method for analyzing hallucinogens, synthetic cathinones, and synthetic cannabinoids in urine was developed. Urine samples were extracted using a liquid-liquid extraction protocol optimized via a multivariate experimental design.

P2X7 receptor antagonism suppresses epileptiform-like activity in an inflammation-primed human iPSC-derived neuron model of drug-resistant epilepsy.

Background And Purpose: Neuroinflammation is increasingly recognised to contribute to drug-resistant epilepsy. Activation of ATP-gated P2X7 receptors has emerged as an important upstream mechanism, and increased P2X7 receptor expression is present in the seizure focus in rodent models and patients. Pharmacological antagonists of P2X7 receptors attenuate seizures in rodents, but this has not been explored in human neural networks.

LONP1 Variants Are Associated With Clinically Diverse Phenotypes.

LONP1 encodes a mitochondrial protease essential for protein quality control and metabolism. Variants in LONP1 are associated with a diverse and expanding spectrum of disorders, including Cerebral, Ocular, Dental, Auricular, and Skeletal anomalies syndrome (CODAS), congenital diaphragmatic hernia (CDH), and neurodevelopmental disorders (NDD), with some individuals exhibiting features of mitochondrial encephalopathy. We report 16 novel LONP1 variants identified in 16 individuals (11 with NDD, 5 with CDH), further expanding the clinical spectrum.

Cardiovascular Safety of COVID-19 Treatments: A Disproportionality Analysis of Adverse Event Reports from the WHO VigiBase.

Introduction: The high mortality of Coronavirus Disease 2019 (COVID-19) highlights the need for safe and effective antiviral treatment. Small molecular antivirals (remdesivir, molnupiravir, nirmatrelvir/ritonavir) and immunomodulators (baricitinib, tocilizumab) have been developed or repurposed to suppress viral replication and ameliorate cytokine storms, respectively. Despite U.

Green synthesis of silver nanoparticles using Ocimum sanctum for efficient Congo red dye removal: a response surface methodology approach.

Synthetic dyes, such as Congo red (CR), pose serious threats to human health and aquatic ecosystems because of their carcinogenicity and resistance to degradation, necessitating the development of efficient and eco-friendly remediation strategies. In this study, silver nanoparticles (AgNPs) were synthesized via a green method using Ocimum sanctum (holy basil) leaf extract and applied for CR dye removal from aqueous solutions. The adsorption process was optimized using response surface methodology (RSM) based on Box-Behnken design (BBD), evaluating the influence of key parameters including pH, AgNP dosage, initial dye concentration, contact time, and temperature.

ASO Practice Guidelines Series: Soft Tissue Sarcoma of the Extremities and Superficial Trunk.

Soft tissue sarcomas (STS) are a heterogeneous group of rare malignant tumors arising from mesenchymal tissues, with extremity and superficial trunk STS (eSTS) comprising the majority of cases. The management of localized eSTS requires a multidisciplinary approach to optimize oncologic and functional outcomes. This review outlines the natural history, diagnostic workup, and treatment principles for localized eSTS, emphasizing the role of histology-specific considerations in guiding management strategies.

The impact of melatonin-enriched media on epigenetic and perinatal changes induced by embryo culture in a mouse model.

Purpose: To determine if melatonin-enriched culture media could offset loss of imprinting in mouse concepti.

Methods: Zygotes were cultured to blastocyst stage under optimized conditions in melatonin-supplemented media at either 10 M (MT 10) or 10 M (MT 10), or without supplementation (Culture + embryo transfer, or ET, positive control). Blastocysts were also developed in vivo (ET negative control).

A simplified clinical frailty scale predicts mortality in emergency department patients with acute dyspnea.

To evaluate a simplified version of the Clinical Frailty Scale (SCFS) among older adults presenting to the emergency department (ED) with acute dyspnea. In this retrospective single-center cohort study, we included patients from the Acute Dyspnea Study (ADYS) cohort. Severity of illness was assessed using the Medical Emergency Triage and Treatment System (METTS).

Phenotypic age acceleration as a novel predictor of benign prostatic hyperplasia: a prospective cohort study.

This study aims to investigate the predictive value of combined phenotypic age and phenotypic age acceleration (PhenoAgeAccel) for benign prostatic hyperplasia (BPH) and develop a machine learning-based risk prediction model to inform precision prevention and clinical management strategies. The study analyzed data from 784 male participants in the US National Health and Nutrition Examination Survey (NHANES, 2001-2008). Phenotypic age was derived from chronological age and nine serum biomarkers.

Evaluation of the efficiency of cyclodextrin polymers as sustainable sampling material for catching palytoxin-like compounds in seawater.

Palytoxin-like compounds, including ovatoxins, are potent emerging toxins responsible for human respiratory poisonings following inhalation of contaminated marine aerosols. Periodic massive proliferations of the ovatoxin-producing organism (Ostreopsis cf. ovata) worldwide, particularly in the Mediterranean, have caused severe toxic outbreaks, drawing the attention of health authorities.

A comprehensive guide to digital neurology. An update on current development on digital neurology derived from the Digital Neuro Hub project.

The rapid evolution of digital tools in recent years after COVID-19 pandemic has transformed diagnostic and therapeutic practice in neurology. This shift has highlighted the urgent need to integrate digital competencies into the training of future specialists. Key innovations such as telemedicine, artificial intelligence, and wearable health technologies have become central to improving healthcare delivery and accessibility.

"Dozing off" in the car and excessive daytime sleepiness (EDS) in Parkinson's disease: a survey of 125 patients.

Parkinson's disease patients are at increased risk of road traffic and car accidents and those with excessive daytime sleepiness are specially susceptible. Abnormal scores on the Epworth Sleepiness Scale predicts risk for driving-related somnolence which may cause road traffic accidents in driving patients as many such patients declare dozing of while in a car. Our study estimates that over 40% of patients with daytime somnolence have risks of dozing off in a car.

Harnessing CoO/AgMoO/CeO ternary nanocomposites for solar light-induced degradation of anthropogenic dye contaminants.

A CoO/AgMoO/CeOternary nanocomposites photocatalyst was successfully synthesized through a straightforward ethanol-assisted chemical method. Comprehensive characterization of its structural and optical properties was conducted using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-Vis diffuse reflectance spectroscopy (UV-DRS), and photoluminescence (PL) analysis. XRD analysis confirmed the presence of CoO, AgMoO and CeO in the ternary composite sample.