Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids SCBG Huihuang90.

This study successfully established an efficient in vitro propagation system for SCBG Huihuang90 via protocorm-like body (PLB) formation from seed-derived calluses, PLB proliferation and differentiation, root induction and greenhouse acclimatization. Furthermore, 1/2 Murashige and Skoog (MS) + 0.025 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) was suitable for the proliferation of PLBs, and 1/2MS + 10% coconut water (CW, /) + 0.

First report of Duverger, 1994 (Coleoptera, Coccinellidae, Diomini) predating on papaya mealybug from China.

Background: Duverger, 1994, an adventive species, is recorded from Guangzhou (Guangdong Province), China for the first time. Larvae of were collected in association with an invasive mealybug, Williams & Granara de Willink, 1992, infesting papayas, cassava and several ornamental plants. However, little has been known about the biology of , especially the morphology of their larvae since their original descriptions.

Gibberellic Acid Inhibits - Symbiosis by Regulating the Expression of Cell Wall Metabolism Genes.

Orchid seeds lack endosperms and depend on mycorrhizal fungi for germination and nutrition acquisition under natural conditions. is a mycorrhizal fungus that promotes seed germination and seedling development in epiphytic orchids, such as . To understand the impact of on seed germination, we examined endogenous hormone levels by using liquid chromatography-mass spectrometry.

Insights into the reduction of methylmercury accumulation in rice grains through biochar application: Hg transformation, isotope fractionation, and transcriptomic analysis.

Methylmercury (MeHg), a potent neurotoxin, easily moves from the soil into rice plants and subsequently accumulates within the grains. Although biochar can reduce MeHg accumulation in rice grains, the precise mechanism underlying biochar-mediated responses to mercury (Hg) stress, specifically regarding MeHg accumulation in rice, remains poorly understood. In the current study, we employed a 4% biochar amendment to remediate Hg-contaminated paddy soil, elucidate the impacts of biochar on MeHg accumulation through a comprehensive analysis involving Hg isotopic fractionation and transcriptomic analyses.

Comparative Analyses of Chloroplast Genome Provide Effective Molecular Markers for Species and Cultivar Identification in .

is popular in ornamental horticulture for its colorful bracts and excellent adaptability, but the complex genetic relationship among this genus is fuzzy due to limited genomic data. To reveal more genomic resources of , we sequenced and assembled the complete chloroplast (cp) genome sequences of 'Splendens'. The cp genome size was 154,869 bp in length, containing 86 protein-coding genes, 38 tRNAs, and eight rRNAs.

 (Lythraceae), a new species from China.

, a new species from southeastern Shaanxi Province and northwestern Hubei Province of China is described. Morphologically, resembles , but it differs in having 4-angular, subalate branchlets, elliptic-lanceolate, or narrowly elliptic leaves, and relatively larger flowers.

Long non-coding RNA-mediated competing endogenous RNA regulatory network during flower development and color formation in .

, an evergreen shrubby flower known for its superior adaptation ability in South China, has gained increased attention in garden applications. However, scant attention has been paid to its flower development and color formation process at the non-coding RNA level. To fill this gap, we conducted a comprehensive analysis based on long non-coding RNA sequencing (lncRNA-seq), RNA-seq, small RNA sequencing (sRNA-seq), and widely targeted metabolome detection of three different flower developmental stages of .

Genome-wide identification and expression profiling of B3 transcription factor genes in .

B3-domain containing transcription factors (TFs) are well known to play important roles in various developmental processes, including embryogenesis, seed germination, etc. Characterizations and functional studies of the B3 TF superfamily in poplar are still limited, especially on their roles in wood formation. In this study, we conducted comprehensive bioinformatics and expression analysis of B3 TF genes in .

Precipitation regulates the responses of xylem phenology of two dominant tree species to temperature in arid and semi-arid forest of the southern Altai Mountains.

Arid and semi-arid forests are important carbon sinks, with implications for the global carbon balance. However, the impacts of climate warming on the growth of arid and semi-arid forest tree species and ecosystem carbon sink dynamics remain uncertain because the effects of the complex interactions between precipitation and temperature on xylem phenology are not clearly understood. Here, we monitored xylem formation over two years in two dominant tree species (Siberian larch, Larix sibirica Ledeb.

The transcriptome of Icerya aegyptiaca (Hemiptera: Monophlebidae) and comparison with neococcoids reveal genetic clues of evolution in the scale insects.

Background: Scale insects are worldwide sap-sucking parasites, which can be distinguished into neococcoids and non-neococcoids. Neococcoids are monophyletic with a peculiar reproductive system, paternal genome elimination (PGE). Different with neococcoids, Iceryini, a tribe in non-neococcoids including several damaging pests, has abdominal spiracles, compound eyes in males, relatively abundant wax, unique hermaphrodite system, and specific symbionts.

T-DNA insertion mutagenesis in results in identification of an enolase gene mutant impaired in secretion of organic acids and phosphate solubilization.

strain P6 is a phosphate-solubilizing fungus isolated from farmland in Guangdong Province, China. To gain better insights into the phosphate solubilization mechanisms of strain P6, a T-DNA insertion population containing approximately 4500 transformants was generated by -mediated transformation. The transformation procedure was optimized by using a Hybond N membrane for co-cultivation of and .

A review of the genus Sasajiscymnus Vandenberg, 2004 from China (Coleoptera, Coccinellidae).

In the present study, the taxonomy of the genus Sasajiscymnus Vandenberg, 2004 from China is revised. The number of Chinese species of Sasajiscymnus was increased from thirty-five to sixty-four. Twenty-seven species are newly described: S.

Functional characterization of three TERMINAL FLOWER 1-like genes from Platanus acerifolia.

TFL1-like genes of the basal eudicot Platanus acerifolia have conserved roles in maintaining vegetative growth and inhibiting flowering, but may act through distinct regulatory mechanism. Three TERMINAL FLOWER 1 (TFL1)-like genes were isolated and characterized from London plane tree (Platanus acerifolia). All genes have conserved genomic organization and characteristic of the phosphatidylethanolamine-binding protein (PEBP) family.

Ecological network construction and optimization in Guangzhou from the perspective of biodiversity conservation.

Rapid urbanization has resulted in fragmentation and decreased connectivity in urban landscapes, endangering regional biodiversity conservation and high-quality, sustainable development. networks (ENs) construction and optimization is a critical technique to enhance landscape connectivity effectively, promote species interaction and dispersal, and thereby hence urban biodiversity., most existing studies on ENs construction have been undertaken from the perspective of ecosystem service functions or the value of landscape structure, with regional species still being overlooked.

Effect of Climate Change on the Potentially Suitable Distribution Pattern of Miq. in China.

Climate warming poses a great threat to ecosystems worldwide, which significantly affects the geographical distribution and suitable growth area of species. Taking Miq. as the research object, the potentially suitable cultivation regions under present and future climatic emission scenarios in China were predicted based on the MaxEnt model with 360 effective individual distributions and eight environmental variables.

Chromosomal-level genome assembly of provides insights into trichome evolution.

, consisting of ~100 species diversified in tropical Asia and Oceania in the past 1-2 million years, represents an excellent example of rapid speciation in flowering plants. Trichomes on hypanthia, twigs and leaves vary markedly among species of this genus and are the most important diagnostic traits for species identification. These traits also play critical roles in contributing to differential adaptation of these species to their own habitats.

Genome-wide identification of members of the family in almond (), cloning and expression characterization of .

Unlabelled: (S-phase kinase-associated protein 1) is the core gene of SCF ubiquitin ligase, which mediates protein degradation, thereby regulating biological processes such as cell cycle progression, transcriptional regulation, and signal transduction. A variety of plant gene family studies have been reported. However, the almond gene family has not yet been studied.

Identification and validation of reference genes for qRT-PCR analyses under different experimental conditions in Allium wallichii.

Himalayan onion (Allium wallichii) is a perennial bulbous herb with high ornamental value and has long been used as traditional medicines in Nepal and China because of the anti-cancer and anti-microbial activities. Wild Allium wallichii features different flower colors, including purple, pink, deep purple and white. However, little is known about the molecular mechanisms of color formation during A.

Genome-Wide Identification and Characterization of Gene Family Members in .

It has been confirmed that the plant-specific Teosinte-branched 1/Cycloidea/Proliferating (TCP) gene family plays a pivotal role during plant growth and development. M. candidum is a native ornamental species and has a wide range of pharmacodynamic effects.

A Taxonomic Review of the Genus Casey (Coleoptera, Coccinellidae) from China, with Descriptions of Eight New Species.

Twenty species of the genus Telsimia from China are reviewed herein. Among them, eight species are described as new to science: Telsimia chayuensis, T. forcipata, T.

Genome-Wide Analysis of the Almond Superfamily and Its Functional Prediction during Dormancy in Response to Freezing Stress.

The AP2/ERF transcription factor family is one of the largest transcription factor families in plants and plays an important role in regulating plant growth and development and the response to biotic and abiotic stresses. However, there is no report on the AP2/ERF gene family in almond (). In this study, a total of 136 genes were identified from the almond genome, and their protein physicochemical properties were analyzed.

Characterization of the gene family reveals its contribution to the adaptability of almond ().

Background: WRKY (WRKY DNA-binding domain) transcription factors an important gene family that widely regulates plant resistance to biological and abiotic stresses, such as drought, salt and ion stresses. However, research on the family in almond has not yet been reported. Almond is an economically important fruit tree in Xinjiang that have strong resistance to various stresses.

Chromosomal-level genome assembly of the orchid tree Bauhinia variegata (Leguminosae; Cercidoideae) supports the allotetraploid origin hypothesis of Bauhinia.

Cercidoideae, one of the six subfamilies of Leguminosae, contains one genus Cercis with its chromosome number 2n = 14 and all other genera with 2n = 28. An allotetraploid origin hypothesis for the common ancestor of non-Cercis genera in this subfamily has been proposed; however, no chromosome-level genomes from Cercidoideae have been available to test this hypothesis. Here, we conducted a chromosome-level genome assembly of Bauhinia variegata to test this hypothesis.

Comparative Analysis of the Complete Chloroplast Genomes of Nine Species.

is known as "lady's or Venus" slipper orchids due to its prominent shoe-shaped labellum, with high ornamental value. Phylogenetic relationships among some species in genus cannot be effectively determined by morphological features alone or through the analysis of nuclear or chloroplast DNA fragments. In order to provide aid in understanding the evolutionary and phylogenetic relationship in at chloroplast (cp) genome-scale level, the complete cp genomes of six species were newly sequenced in this study, and three other published cp genome sequences of were included in the comparative analyses.

GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite Shiraki Digesting the Weed Kunth.

Effective approaches to exploiting the biomass of the abundant invasive weed Kunth are limited. Termites have been a focus of significant attention as mediators of biomass-processing owing to their ability to digest lignocellulose. Here, the GC/TOF-MS approach was employed to assess the effects of a diet composed of leaves on workers, with the growth performance of these workers also being assessed.